Nanoparticle - Fundamentals and applications, formulation, preparation methods and evaluation.

1. NANOPARTICLES

2. Nanoparticles are solid, colloidal particles ranging 10-
1000nm, consisting of macromolecular materials in which
active ingredient is dissolved/ entrapped/ encapsulated/
absorbed/ attached.

3. Formulation of nanoparticles
Polymers:
1) Natural, 2) Semi-Synthetic, 3) Synthetic

4. Semisynthetic polymers:
• Pseudolatexes/ artificial latexes obtained form
dispersion of preformed polymers.
Ex: pseudo latexes of ethylcellulose, CAP etc.,
• These are used in preparation of nanocapsules.

31. (Acetone)
Salting out agent(MgCl, NaCl)
To collect separated acetone

33. To remove organic solvents, electrolytes, surfactants, stabilizers
& large polymer aggregates.

38. Evaluation of nanoparticles
1. Nanoparticle recovery & drug incorporation efficiency
2. Size & morphology
3. Specific surface
4. Surface charge & eletrophoretic mobility
5. Surface hydrophobicity
6. Density
7. Molecular weight
8. Chemical analysis
9. Protein adsorption
10.Biodegradation
11.In-vitro drug release.

39. Nanoparticle recovery & drug incorporation efficiency

40. Size & Morphology

41. Specific surface Sarptometer
Surface charge &
eletrophoretic
mobility
Laser Doppler Anemometry & Velocimetry
Surface
hydrophobicity
Hydrophobic interaction chromatography, 2-
phase partition, adsorption of hydrophobic
radiolabelled or fluorescent probes & conact
angle measurement.
Density Helium or air using a gas Pycnometer.
Molecular weight Gel permeation chromatography using refractive
index detector.
Chemical analysis X-ray photo electron spectroscopy
Protein adsorption 2D- PAGE (Polyacrylamide Gel Electrophoresis)
Biodegradation Nephelometer

42. In-vitro drug release:
1. side-by-side diffusion cells with artificial/ biological
membrane
2. Dialysis bag diffusion technique
3. Reverse dialysis sac technique
4. Ultracentrifugation
5. Centrifugal ultra filtration technique.

45. Thank
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