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Application of analytical techniques to preserve the biodiversity and ecological services of wetland

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Presented by Dr. Tran Thi Nhu Trang, Faculty of Environmental and Food Engineering, NTTU, on 10 November 2020 at "International workshop: Enhancing wetland management and sustainable development"

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Application of analytical techniques to preserve the biodiversity and ecological services of wetland

  1. 1. THE INTERNATIONAL WORKSHOP ON “ENHANCING WETLAND MANAGEMENT AND SUSTAINABLE DEVELOPMENT” Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam November 10th, 2020 Application of analytical techniques to preserve the biodiversity and ecological services of wetland Ứng dụng kỹ thuật phân tích để bảo tồn đa dạng sinh học và các dịch vụ sinh thái của đất ngập nước Ms. Tran Thi Nhu Trang, Ph.D. Faculty of Environmental and Food Engineering Nguyen Tat Thanh University, Vietnam Email: ttntrang@ntt.edu.vn
  2. 2. UNIVERSITY ACADEMICS HEALTH SCIENCES ECONOMICS & MANAGEMENT ENGINEERING - TECHNOLOGY SOCIAL SCIENCES & HUMANITIES ARTS – FINE ARTS FACULTY OF ENVIRONMENTAL AND FOOD ENGINEERING
  3. 3. ACADEMIC TEACHING – FACULTY OF ENVIRONMENTAL AND FOOD ENGINEERING (NTTU) CHEMICAL ENGINEERING FOOD TECHNOLOGY ENVIRONMENTAL MANAGEMENT AND ENGINEERING • General chemistry • Thermochemistry • Processing and equipment • Natural compounds • Analytical chemistry • Process fluid mechanics • Chemical process and control • Fundamentals of heat and mass transfer • Chemical process design • Chemistry of surfactants • Food chemistry • Food packaging technology • Food additives • Food processing technology • Food analysis • Experimental design and data analysis • Computer application in food science and technology • Food microbiology • Environmental analysis • Environmental technology • Soil pollution and treatment • Ecotoxicology • Basic environmental engineering • Water pollution and treatment • Sustainable energy • Climate Change • Environmental protection in urban area • Environmental management system
  4. 4. Quality Analysis and Authenticity of Food Figure. Major technologies for the detection of food fraud, as reported in the literature from 2005 to 2015 Hong et al., J Sci Food Agric, 97, 3877–3896 (2017)
  5. 5. Origins of REIMS  Rapid Evaporative Ionisation Mass Spectrometry (REIMS) is an emerging technique that allows rapid characterisation of biological tissues with no need for sample preparation  The inventor, Professor Zoltan Takats of Imperial College, London, created the iKnife, by connecting an electrosurgical knife to a mass spectrometer – Electrosurgical knives use an electrical current to rapidly heat tissue, cutting through it while minimising blood loss – In doing so, they vaporise the tissue, creating smoke that is normally sucked away by extraction systems but is actually a rich source of biological information that can provide the surgeon a real-time decision-making tool
  6. 6. + - - + - -+ - - Gas phase ions - - + Rapid Evaporative Ionisation Mass Spectrometry Principle iknifeRapid diathermic process - gas phase ions formed at the tissue surface Declustering on impact with heated electrode surface Bipolar forceps
  7. 7. REIMS for food research workflow chemometric profiling iKnife and Xevo G2-XS QTof Model Generation Multivariate statistical analysis Different tissues Different species Different production Biomarker ID & verification
  8. 8. Isotopic analysis Element Isotope Mass Relative natural abondance (%) Hydrogen major 1H 1.007825 99.984 minor 2H or D 2.014102 0.0156 Carbone major 12C 12.00000 98.891 minor 13C 13.00335 1.108 Nitrogen major 14N 14.003074 99.635 minor 15N 15.000109 0.365 Oxygène Major 16O 15.994915 99.759 Minor 17O 16.999131 0.037 18O 17.999160 0.204 Sulfur Major 32S 31.972072 95.02 Minor 33S 32.971459 0.75 34S 33.967868 4.21 36S 35.967079 0.02
  9. 9. Element Isotope Mass Relative natural abondance (%) Hydrogen major 1H 1.007825 99.984 minor 2H or D 2.014102 0.0156 Carbone major 12C 12.00000 98.891 minor 13C 13.00335 1.108 VERY LOW NATURAL QUANTITIES Analytical technique must be very precise International Standard Internal Standards of Laboratory Isotopic analysis
  10. 10. (13C/12C)samp - (13C/12C)PDB (13C/12C)PDB PDB : Pee Dee Belemnite (13C/12C)PDB = 0.0112372 d13Csamp/PDB (‰) = x 1000 (2H/1H)samp - (2H/1H)V-SMOW (2H/1H)V-SMOW V-SMOW : Vienna standard mean ocean water (2H/1H)V-SMOW = 155.76  10-6 d2Hsamp/V-SMOW (‰) = x 1000 Isotopic analysis (15N/14N)samp - (15N/14N)air (15N/14N)air d15Nsamp/air (‰) = x 1000
  11. 11. source of carbon for terrestrial plants 3 groups of photosynthesis Calvin Cycle C3 Hatch and Slack Cycle C4 Crassulacean Acid Metabolisme CAM source of hydrogen for plants H2O Isotopic analysis
  12. 12. Distribution of d13CPDB values in nature (Meier-Augenstein, 1999) C4 -9 to -17‰C3 -24 to -34‰ CAM -10 to -25‰ < -22‰  Isotopic analysis
  13. 13. Distribution of d2HV-SMOW values according to the latitude Relation with the geographical parameters  latitude  distance to the sea  altitude  temperature  moisture  sunning time  quantity of precipitation  Isotopic analysis
  14. 14. Isotopic analysis Fig. a) δ13C and b) δ15N of SPM expressed as a function of salinity in the Can Gio mangrove estuary. Letters in bubbles correspond to the sites where samples were collected. Fig. 1. Map of the Can Gio mangrove estuary (Southern Vietnam). A, B, C and D indicate the sampling stations along the estuary. F. David, C. Marchandb,c, N. Thineya, T.T. Nhu-Trang, T. Meziane, Continental Shelf Research, 178, 1, 59-67 (2019)
  15. 15. PROJECT IAEA/RCA RAS7037 ENHANCING WETLAND MANAGEMENT AND SUSTAINABLE CONSERVATION PLANNING (2020 - 2024)
  16. 16. Project team and background • A project team has been established with  Dr. Tran-Thi Nhu-Trang of Nguyen Tat Thanh University (NTTU) as NPC  NTTU as the lead institute  Institute for Nuclear Science and Technology (INST) - VINATOM will also participate in the project  List of national team members: • Dr. Nguyen Thanh-Nho, NTTU • Dr. Truong Van Vinh, NLU • Dr. Nguyen Thi Kim Cuc, TLU • Dr. Nguyen Thi Hong Thinh, INST-VINATOM • Dr. Trinh Anh Duc, VINATOM
  17. 17. Stable isotope analysis (δ13C and δ15N) has been used:  To identify organic carbon sources in surface sediments  To study food sources of a fish assemblage  To determine the contribution of mangrove materials and other organic carbon sources to the invertebrate community  To calculate dating of sediment from estuarine dominated mangrove  To assess the quality change of mangrove leaf litter during its decomposition Country situation analysis
  18. 18. • What are our goals? 1.To enhance the technical capacity in the interpretation of isotopic data for wetland management and conservation. 2.To enhance the country’s intermediate and advanced knowledge of science and technology. 3.To establish regional partnerships to better coordinate scientific research in wetland. 4.To assess the role of wetland ecosystems (i.e. mangrove forest) in the ability to accumulate and reduce the content of pollutants (metalloids) in the river water flowing through the forest system. 5.To fill knowledge gaps and provide data analysis to MARD for the revision and implementation of PFES at provincial and national levels. 6.To build a database of wild-fish stable isotope signatures that would assist in seafood provenance studies throughout the region. Country situation analysis
  19. 19. THANK YOU Ms. Tran Thi Nhu Trang, Ph.D. +84 909 008 162 ttntrang@ntt.edu.vn www.ntt.edu.vn FACULTY OF ENVIRONMENTAL AND FOOD ENGINEERING (ENFO-NTTU)

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