Citrex610 m-en-citrex carne

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CITREX

MICROORGANISMS INVESTIGATED ,[object Object],[object Object],[object Object],[object Object],[object Object]

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Nutritional factors influencing value of forages used as substrates for silage

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Bacteriocin based strategy for enhaced food safety

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IOSR Journals

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CITREX

Fermentation of Penicillin Antibiotic Penicillin is an antibiotic produced by microorganisms. These antibiotics inhibit the growth and development of another micro-organism. Generally, the penicillin antibiotic is produced by some actinomycetes and some filamentous fungi. The antibiotics produced by these micro-organisms can be used medicine field, veterinary as well as agricultural field. Penicillin antibiotic was the first antibiotic used in large amount during world war second for treatment of soldiers. Penicillin is a antibiotic used against Gram positive bacteria as well as high dosage can be used against Gram negative bacteria. Penicillin is not harmful to plants, animals or human beings except in some cases of allergies

Fermentation of penicillin antibiotic

VivaMariyaVarghese

In the present investigation partially purified alkaline protease from Aspergillus sp. As#6 and As#7 strains were entrapped in calcium alginate beads and characterized using casein as a substrate. Temperature and pH maxima of protease from As#6 strain showed no changes before and after immobilization and remained stable at 450C and pH 9, respectively. However km value was slightly shifted from 4.5mg/ml to 5 mg/ml. Proteases from As#7 strain showed shifting in pH optima to a more alkaline range (10.0) as compared with free enzyme (9.0). Optimum temperature for protease from As#7 strain showed changes after immobilization and shifted from 650C to 850C. However there was no significant effect on Km value but Vmax of immobilized protease from As#7 strain was also shifted from 200U/ml to 370U/ml. Immobilized protease from As#6 strain was reused for 3 cycles with 22% loss in its activity whereas immobilize protease from As#7 strain was reused for 3 cycles with 17% loss in its activity. Protease from As#7 strain has a higher affinity for the substrate and higher proteolysis activity than protease from As#6 strain. The present work concludes that Aspergillus As#7 strain may be a good source of industrial protease

Kinetic study of free and immobilized protease from Aspergillus sp.

IOSR Journals

Lactic acid bacteria whole genome sequencing

Diwas Pradhan

Dhanalakshmi

International Center for Research & Development

The present study is about the anti-microbial activity of the bacteriocin producing lactobacilli and optimization of bacteriocin production. Bacteriocin was extracted by solvent extraction with chloroform and the antimicrobial activity was tested against 5 different pathogens by agar spotting method. Optimization of bacteriocin production was done for 4 different parameters such as pH, Temperature, Carbon source and Nitrogen source and the anti-microbial activity was tested against the following 5 different pathogens and the results were observed and diameter of the zone of inhibition was measured and tabulated. From the results of the study it was found that bacteriocin produced from lactobacilli has good antimicrobial activity.The present study is about the anti-microbial activity of the bacteriocin producing lactobacilli and optimization of bacteriocin production. Bacteriocin was extracted by solvent extraction with chloroform and the antimicrobial activity was tested against 5 different pathogens by agar spotting method. Optimization of bacteriocin production was done for 4 different parameters such as pH, Temperature, Carbon source and Nitrogen source and the anti-microbial activity was tested against the following 5 different pathogens and the results were observed and diameter of the zone of inhibition was measured and tabulated. From the results of the study it was found that bacteriocin produced from lactobacilli has good antimicrobial activity.

Anti-Microbial Activity of probiotic Lactobacilli and Optimization of Bacteri...

ijsrd.com

The current studies were aimed at to investigate role of pH, dissolved oxygen for production protease in bioreactor by alkalophilic bacterium and application of saw dust for its purification. The production of proteolytic enzyme by Bacillus subtilis IC-5 started as pH of medium falls to 9 and reached to maximum at pH 7 i.e., 4400 Uml-1 . Likewise dissolved oxygen decreased in the medium as the protease production progresses. Saw dust was successively utilized for partial purification of protease. The partial purification of protease increased the specific activity to5.3 fold. The optimum pH and temperature for purified activity was 11 and 700C, respectively. The purified enzyme was stable up to pH 12 and 80oC.

PRODUCTION OF PROTEASE BY ALKALOPHILIC BACILLUSSUBTILIS IN BIOREACTOR AND ITS...

AM Publications

Biopreservatives

Punjabi university

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IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) covers studies of the chemical processes in living organisms, structure and function of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules, chemical properties of important biological molecules, like proteins, in particular the chemistry of enzyme-catalyzed reactions, genetic code (DNA, RNA), protein synthesis, cell membrane transport, and signal transduction. IOSR-JBB is privileged to focus on a wide range of biotechnology as well as high quality articles on genetic engineering, cell and tissue culture technologies, genetics, microbiology, molecular biology, biochemistry, embryology, cell biology, chemical engineering, bioprocess engineering, information technology, biorobotics.

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Nutritional factors influencing value of forages used as substrates for silage

Bacteriocin based strategy for enhaced food safety

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Isolation of Bacteriocin Producing Lactobacillus SPP from fermented food like...

Citrex camaron eng

Fermentation of penicillin antibiotic

Kinetic study of free and immobilized protease from Aspergillus sp.

Lactic acid bacteria whole genome sequencing

Dhanalakshmi

Anti-Microbial Activity of probiotic Lactobacilli and Optimization of Bacteri...

PRODUCTION OF PROTEASE BY ALKALOPHILIC BACILLUSSUBTILIS IN BIOREACTOR AND ITS...

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Isolation , characterization and comparative study of lactobacillus sp. using...

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Preservative potentials of crude bacteriocins produced by Lactobacillus tucce...

Production of antibiotics

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Isolation of protease producing microorganism

Safety assessment of probiotics for human use

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Microbial enzymes have shown tremendous potential for different applications. Over the years due to their remarkable features enzymes have occupied the centre stage of all the biochemical and industrial processes. Pectinases are a group of enzymes responsible for the hydrolysis of pectic materials found in plants and are important industrial enzymes. In the present study, pectinase is produced from Bacillus sp. that was isolated from vegetable waste dump soil samples. A total of five isolates showed pectinase production and designated as PPB1 to PPB5. The screened isolates were used as a source of pectinase production using cassava waste as a substrate. Isolate PPB5 showed maximum enzyme activity of 0.641 IU/ml. Pectinase activity was optimized for various parameters like incubation time, temperature, pH, different carbon and nitrogen sources. Enzyme activity was observed maximum at 96 hr of incubation, 35°C temperature and at pH 6. The best carbon was found to be glucose. Among organic and inorganic nitrogen sources yeast extract and ammonium nitrate was founded to be better than other nitrogen sources. Among the five isolates, the isolate PPB5 showed maximum activity at all optimum conditions. This isolate is best producer and can be used in future for further pectinase production.

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This study was conducted to study the effects of supplementation alfalfa silage with orange pulp and difference of Lactobacillus buchneri on in vitro dry matter digestibility and gas production. wilted alfalfa with no additive (control), wilted Alfalfa and orange pulp (1750 g wilted Alfalfa mixed with 750 g fresh orange pulp) treated with LAB for final application rates of 0, 2.5, 5 and 7.5 g LAB inoculant/ton of wilted alfalfa and orange pulp (LAB0, LAB1, LAB2, LAB3, respectively). Alfalfa hay harvested at flowering stage and after 24 hours wilted and mixed orange pomace with ratio of 2100 g and 760 g, respectively, and was ensiled for 90 days. The data were analyzed in a completely randomized design with three replications. After 24 h incubation, treatments AO (alfalfa + orange pulp) and CON (without additive) had the highest and lowest in vitro gas production (p<0.05) and adding orange pulp and molasses increased gas production. Adding inoculant decreased in vitro DM digestibility. Results showed that ensiling alfalfa with orange pulp and molasses can improved silage quality and increased gas production and in vitro DM digestibility.

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Caesalpinia sappan L (Sappanwood) contains antibacterial compounds and antioxidants that inhibit the growth of microbes. This study aimed to investigatethe microbiological and physicochemical qualities of pasteurized milk supplemented with 0, 2, 4, 6 and 8% (w/v) sappan wood extract. Data were analyzed using a completely randomized design factorial followed by the Duncan’s new multiple range test. Preliminary analysis showed that sappan wood extract contained 44.66 ± 0.09 mg/100g phenols, 0.18 ± 0.01 mg/100mg flavonoids, 46.42 ± 0.23 mg/100g tannins, and antioxidant activity at 85.82 ± 0.25%. The addition of sappan wood extract significantly increased the antioxidant activity (P<0.05) of pasteurized milk during storage. Pasteurized milk supplemented with sappan wood extract had a lower total bacterial count (P<0.05) than that of unsupplemented pasteurized milk, and supplemented milk showed strong antibacterial activities against Escherichia coli, Shigella flexneri, Salmonella thypimurium, Staphylococcus aureus, and Listeria monocytogenes.The addition of sappan wood slightly increased the protein content but did not affect pH, and viscosity. It is concluded that the addition of sappan wood extract increased the microbiological quality and maintained the physicochemical quality of pasteurized milk, thus extending the product’s shelf-life.

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