Chromatography Products Catalog 2014

Chromatography and Laboratory Products 2014

Phone: 866-SORBTEC (767-2832)
Fax: 770-936-0326
Email: sales@sorbtech.com
Online: www.sorbtech.com
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• Sorbent Technologies, Inc. warrants its products to be free from known defects and shall conform to the description and specifications of
such products as specified on the label, and in the catalog, data sheets, or literature at the time of the sale. Specifications are subject to change
without notice.
• Sorbent Technologies, Inc.’s warranty is strictly limited to the value of the product sold.
• This warranty is exclusive, and Sorbent Technologies, Inc. makes no other warranty, express or implied, including without limitation any implied
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Copyright ©2014 Sorbent Technologies, Inc. All rights reserved.
Ordering - Sales, Terms, & Conditions
Mail: Sorbent Technologies, Inc.
5955 Peachtree Corners East
Suite A
Norcross, GA 30071

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www.sorbtech.comSorbent Technologies, Inc.
Providing Purity and Performance, Service and Selection
www.sorbtech.com
Visit our website for our complete product selection
and to search our application database.
S O R B T E C HS O R B E N T T E C H N O L O G I E S
Page
About Sorbent Technologies Inc.......................................................................4
Silica Gel ......................................................................................................................5
Granular ..................................................................................................................6
Silica Gel for ASTM D1319-01 & ISO 3837 ..................................................9
Spherical ...............................................................................................................10
Bonded Phases...................................................................................................13
Dry Column Chromatography ........................................................................ 16
Alumina .................................................................................................................... 18
Alumina Solvent Purification ........................................................................21
Specialty Adsorbents ......................................................................................... 24
Polymeric Resins ................................................................................................... 26
SPE Cartridges ....................................................................................................... 33
CHROMABOND® ................................................................................................33
QuEChERS.............................................................................................................43
Vacuum Manifold...............................................................................................44
Jordi .......................................................................................................................46
TLC Plates ................................................................................................................ 48
Flash Cartridges .................................................................................................... 57
EZ Flash System..................................................................................................... 61
EZ Mini Pump......................................................................................................61
EZ Fraction Collectors.......................................................................................61
EZ Flash Detectors.............................................................................................62
FPLC Columns......................................................................................................... 66
SNAP®.....................................................................................................................66
Econoline® and Econoline® LP.......................................................................68
Upscale & IsoKrom.............................................................................. 69
Syringe Filters......................................................................................................... 70
HPLC Columns........................................................................................................ 72
Sorbtech Purity...................................................................................................72
Macherey-Nagel.................................................................................................73
MCI GEL®...............................................................................................................80
Jordi........................................................................................................................83
HPLC Column Packing System......................................................................... 85
GC Columns............................................................................................................. 86
Laboratory Equipment.................................................................96
Centrifan PE - Personal Evaporator..............................................................96
Variable Speed Rocker and Orbital/Linear Shaker.................................98
Hotplate Stirrers.................................................................................................99
Pipettors and Serilogical Pipets ................................................................100
Pipet Tips............................................................................................................101
Bottle Dispensers/Weigh Dishes/ Cuvettes...........................................102
PCR Products....................................................................................................103
Tubes...................................................................................................................104
Racks and Gloves.............................................................................................105
Microscopes......................................................................................................106

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www.sorbtech.com Sorbent Technologies, Inc.
About Sorbent Technologies, Inc.
Your Success is Our Goal!
When chromatography is the means to the success of weeks or even years of hard work, industry leaders and researchers rely on Sorbent
Technologies’superior products, information and consultation. That is because we are committed to the success of your work and we partner with
our customers to provide the most effective solutions for your projects’unique challenges.
Chromatography is our expertise. Our trained product specialists will assist you with method development and optimization from analytical
methods to full scale process. Furthermore, we provide an extensive applications database to describe methods for LC, HPLC, SPE, TLC and GC
which includes both mobile and stationary phases for your target compound. We understand the importance of your work and the necessity for
high-quality information and products.
This catalog displays a sampling of our product offerings and product information. To find out more about our full line of chromatography
products, visit www.sorbtech.com or contact one of our product specialists at 866-SORBTECH.
Highest Quality
Since 2000 Sorbtech has been providing the highest-quality adsorbents for chromatography and purification from laboratory to pilot to full-scale
production. Manufactured to the highest standards, all of our adsorbents offer:
• The highest purity
• Standardized activities
• Narrow particle and pore distributions
• Consistent particle shape and surface symmetry
• Excellent mechanical and structural properties for pressure stability
• Exceptional bonding techniques

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Silica Gel
Selection Guides
Silica Gel is our specialty. We offer the largest selection of silica gels with pore diameters ranging from 60 Å to 1000 Å, particle sizes from 2 μm to
500 μm, and bonded phases from C18 to SAX (HPLC, MPLC, Flash & Gravity Grades). Sorbent Technologies offers the best selection of granular
silica gels for chromatography including TLC, MPLC, Flash, and Gravity grades. Use the Silica Guides below to help you find the right silica for your
application.
Broadest Selection
Sorbent Technologies offers a full range of standardized granular silica gels in premium and standard grades. Our complete selection of silica
gel products designed to perform simple to complex analytical and preparative chromatographic techniques for laboratory, pilot, and industrial
process applications.
• Granular and Spherical
• Particle sizes for analytical to preparative to process applications from 2.0 μm to 3 mm
• Pore sizes for small to large molecule separations (proteins & peptides) from 60 Å to 1000 Å
• C18, C8, C4, C2, Amino, Cyano, Phenyl, Diol, and Custom Bonded Phases
• Modified aluminas with three pH levels and specific Brockmann activities
• Ion exchange resins with superior physical and chemical properties
With all of these adsorbents available at Sorbtech, finding appropriate adsorbents is easy and convenient. Allow a Sorbtech product specialist to
help you develop or optimize your application or process.
Compound
to be
Purified
Functionality
Chemistry
Solubility
Molecular
Weight
Separation
Difficulty
α < 1.2 1.2 < α < 1.5 1.5 < α < 2.0 α < 2.0
75-200 μm40-75 μm
3 & 5 μm
10 μm
15 μm
20 μm
40 μm
20 - 45 μm
Non Aqueous
Non to Low
Polarity
Silica C4 & C8 C18
60 Å
70 Å
100 Å
200 Å
300 Å
500 Å
800 Å
1000 Å
Low to
Medium
Polarity
Medium to
High Polarity
Aqueous MW<1,000D
Organic
Molecules
MW<10,000D
Peptides
MW<50,000D
Polypeptides
Bio-polymers
Capacity Selectivity
SilicaGel

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Granular Silica Gel - TLC, MPLC, Flash, & Gravity LC Phases
Premium Rf™ silica gel is the finest granular silica gel on the market. This high purity product provides narrow particle and pore distribution and is
designed to increase yield, making it ideal for every-day chromatography and complicated separations. With Premium Rf™ your lab can enjoy bulk
quantities for large-scale applications, no scale-up limitation, and secured lots offered for process use.
Granular or irregular-shaped silica gel is commonly used for the following applications: Medium Pressure Liquid Chromatography (MPLC), Flash
Chromatography, and Classic Column Chromatography. Silica gels used for these applications are normally 60 Å or sometimes 100 Å. For larger
porosities please visit our Spherical Silica Gel pages later on in this catalog and also available online at www.sorbtech.com. For granular bonded
phase silica gel, see the Bonded Phase Section.
Recommended Applications: • Aflatoxins • Chloramphenicol • Pesticides • Steroids • Vitamins
Advantages of Premium Rf™ Flash Silica Gel
1. Narrow particle distribution: 40-75 μm +/- 5% versus leading brands +/- 10-12%.
• Better and easier packing.
• No channeling in the bed, leading to tighter bands, thus better separation.
• Lower pressure drop (fewer fine particles), leading to faster flow, thus faster run time.
2. Small pore volume: Higher bulk density.
• More silica gel per column volume, leading to greater loadability.
• Lower dead volume, leading to less solvent use.
3. Narrow pore size distribution: Minimum small pores.
• Maximum targeted surface area available, leading to greater loadability.
• Minimum risk of product trapped in pores, leading to increased yield and longer life time of the
silica gel.
4. Purity: 99.95% SiO2
• Our high purity (low metal impurities) ensures maximum resolution as compared to the competition.
5. Side by side comparison tests of every major silica gel show our Premium Rf to give the best results.
• Easier to pack • More fractions • Faster run time • Better resolution •Increased yield
Silica Gel - Granular Catalog
No.
Particle
Dist
Porosity pH
Value
LOI Bulk
Density
Surface Area Water Soluble
Matter
Premium Rf 52200 15 - 40 μm 60 Å ~ 7 5% 0.5 g/ml 450-550 m2
/g -
/g -
/g -
/g -
Premium Grade 40930M 40 - 63 μm 60 Å ~ 7 2 - 4% 0.5 g/ml 460-550 m2
/g -
Standard Grade 11610 5 - 15 μm 60 Å ~ 7 - 0.4 g/ml 500-600 m2
/g 0.2%
/g 0.2%
/g 0.2%
/g 0.2%
Standard Grade 30930M 40 - 63 μm 60 Å ~ 7 < 9% 0.5 g/ml 450-550 m2
/g -
Standard Grade 40930 40 - 63 μm 60 Å ~ 7 < 7% 0.5 g/ml 450-550 m2
/g -
/g 0.2%
/g -
/g -
/g 0.2%
/g 0.2%
Standard Grade, Active 10800 18 - 32 μm 60 Å ~ 7 < 5% 0.4 g/ml 500-600 m2
/g 0.2%
/g 0.2%
/g 0.2%
/g 0.2%
/g 0.2%
/g 0.2%
Standard Grade, Active 10860 200 - 500 μm 60 Å ~ 7 < 5% 0.5 g/ml 500-600 m2/g 0.2%
SilicaGel

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Cat. # Product Part. Dist. Size
10910-05 Silica Gel for MPLC, Standard Grade, 60 Å 12-26 μm 500 g
10910-1 Silica Gel for MPLC, Standard Grade, 60 Å 12-26 μm 1 kg
10910-2.5 Silica Gel for MPLC, Standard Grade, 60 Å 12-26 μm 2.5 kg
10920-05 Silica Gel for MPLC, Standard Grade, 60 Å 18-32 μm 500 g
10920-2.5 Silica Gel for MPLC, Standard Grade, 60 Å 18-32 μm 2.5 kg
52300-05 Silica Gel for MPLC, Premium Rf™, 60 Å 20-45 μm 500 g
52300-1 Silica Gel for MPLC, Premium Rf™, 60 Å 20-45 μm 1 kg
52300-2.5 Silica Gel for MPLC, Premium Rf™, 60 Å 20-45 μm 2.5 kg
For more selections and sizes, visit our website or contact your personal Product Specialist.
11610-05 Silica Gel for TLC, Standard Grade, 60 Å 5-15 μm 500 g
11610-25 Silica Gel for TLC, Standard Grade, 60 Å 5-15 μm 25 kg
11620-05 Silica Gel for TLC, Standard Grade, 60 Å, w/UV254 5-15 μm 500 g
11620-25 Silica Gel for TLC, Standard Grade, 60 Å, w/UV254 5-15 μm 25 kg
11630-05 Silica Gel for TLC, Standard Grade, 60 Å, w/ Gypsum 5-15 μm 500 g
11630-25 Silica Gel for TLC, Standard Grade, 60 Å, w/ Gypsum 5-15 μm 25 kg
11640-05 Silica Gel for TLC, Standard Grade, 60 Å, w/ Gypsum and UV254 5-15 μm 500 g
11640-25 Silica Gel for TLC, Standard Grade, 60 Å, w/ Gypsum and UV254 5-15 μm 25 kg
52200-1 Silica Gel for Prep. TLC, Premium Rf™, 60 Å 15-40 μm 1 kg
52200-25 Silica Gel for Prep. TLC, Premium Rf™, 60 Å 15-40 μm 25 kg
11650-1 Silica Gel for Prep. TLC, 60 Å, w/UV254 5-50 μm 1 kg
11650-5 Silica Gel for Prep. TLC, 60 Å, w/UV254 5-50 μm 5 kg
11660-1 Silica Gel for Prep. TLC, 60 Å, w/Gypsum and UV254 5-50 μm 1 kg
11660-5 Silica Gel for Prep. TLC, 60 Å, w/Gypsum and UV254 5-50 μm 5 kg
MPLC Silica Gels - Granular
Improve Your Resolution with MPLC
When working with difficult separations that give very low Rf-values on initial screenings by TLC and require larger volumes of flash grade silica,
you need the improved resolution power that Sorbtech MPLC grades provide. By using a smaller particle size, a higher number of theoretical plates
are achieved using the same volume of silica which in turn increases the resolution.
TLC Silica Gels - Granular
Sorbent Technologies offers an assortment of silica gels for the making of analytical and preparative TLC plates. Our Silica Gel for TLC (Cat. #11610)
and Silica Gel for Prep. TLC (Cat. #52200) contain neither an inorganic nor an organic binder. They can also be used in a packed column format for
extra high resolution.
TLC Slurry preparation for soft layer coating:
1. Silica Gel and small amount of calcium sulfate (gypsum) is mixed with water.
2. This mixture is spread as a thick slurry on a clean glass plate.
3. The resultant plate is dried and activated by heating in an oven for thirty minutes at 110 °C.
The thickness of the absorbent layer is typically around 0.1 – 0.25 mm for analytical purposes and around 0.5 – 2.0 mm for preparative TLC.
SilicaGel

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Flash Silica Gels - Granular
Sorbtech offers high-quality flash and granular silica gels. Our extensive range of silicas have been selected for high performance and optimal
reproducibility while offering a wide range of particle sizes and pore diameters.
* For Reversed-Phased Silica Gels, see Bonded Phase Silica Gels section.
Gravity Silica Gels - Granular
Silica gels for normal phase, classical column chromatography.
Cat. # Product Grade Part. Dist. Size
52500-05 Silica Gel, Premium Rf™, 60 Å Flash 40-75 μm (200x400 mesh) 500 g
52500-1 Silica Gel, Premium Rf™, 60 Å Flash 40-75 μm (200x400 mesh) 1 kg
52500-2.5 Silica Gel, Premium Rf™, 60 Å Flash 40-75 μm (200x400 mesh) 2.5 kg
40930M-05 Silica Gel, Premium, 60 Å Flash 40-63 μm (230x400 mesh) 500 g
40930M-1 Silica Gel, Premium, 60 Å Flash 40-63 μm (230x400 mesh) 1 kg
40930M-2.5 Silica Gel, Premium, 60 Å Flash 40-63 μm (230x400 mesh) 2.5 kg
30930M-05 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 500 g
30930M-1 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 1 kg
30930M-2.5 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 2.5 kg
40930-05 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 500 g
40930-1 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 1 kg
40930-2.5 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 2.5 kg
Cat. # Product Grade Part. Dist. Size
52700-05 Silica Gel, Premium Rf™, 60 Å Gravity 75-200 μm (70x200 mesh) 500 g
52700-1 Silica Gel, Premium Rf™, 60 Å Gravity 75-200 μm (70x200 mesh) 1 kg
52700-2.5 Silica Gel, Premium Rf™, 60 Å Gravity 75-200 μm (70x200 mesh) 2.5 kg
30940-05 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 500 g
30940-1 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 1 kg
30940-2.5 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 2.5 kg
40940-05 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 500 g
40940-2.5 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 2.5 kg
10840-05 Silica Gel, Active, 60 Å Gravity 63-200 μm (70 x 230 mesh) 500 g
SilicaGel

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Item Unit ASTM D1319-02a Results
Year 2002 -
Surface Area m2
/g 430-530 515
pH 5.5-7.0 5.99
Loss of Ignition mass % 4.5-10.0 4.87
Fe2
O3
Mass ppm 50 max -
Particle Size Distribution
on 250 μm 0 0
on 180 μm 1.2 max 0
on 150 μm 5 max 0
75 to 150 μm 1.2 max 94.4
Thru 75 μm 5.6
Pore Volume ml/g 15 max 0.39
H2
O % - 0.66
Laser APS μm - 141.23
SiO2
% - 99.95
Na ppm - 29
Mg ppm - 0.8
Al ppm - 5
Ca ppm - 5.1
Fe ppm - 3.8
Zr ppm - 15
Ti ppm - 102
Cr ppm - 0.18
Mn ppm - 0.02
Co ppm - 0.17
Ni ppm - 0.06
Zn ppm - 0.2
Cd ppm - 0.02
Pb ppm - 0.13
Silica Gel for ASTM D1319-02a and ISO 3037
Quality Silica Gel
Our Silica Gel #51600 is a high purity silica and is manufactured to conform to the specifications of ASTM D1319-02a Test Method for Hydrocarbon
Types in Liquid Petroleum Products by Fluorescent Indicator Adsorption & ISO 3037 Liquid Petroleum Products-Determination of Hydrocarbon
Types-Fluorescent Indicator Adsorption Method. Sorbtech Silica Gel #51600 can be used in place of W.R. Grace’s product (Code 923.)
See table below.
ASTM D1319-02a
This method covers the determination of hydrocarbon types over the concentration ranges from 5 to 99 volume % aromatics, 0.3 to 55 volume %
olefins, and 1 to 95 volume % saturates in petroleum fractions that distill below 315° C.This test method may apply to concentrations outside these
ranges, but the precision has not been determined. Samples containing dark-colored components that interfere in reading the chromatographic
bands cannot be analyzed.
ISO 3037
The determination of the total volume percent [% (V/V)] of saturates, olefins, and aromatics in petroleum fractions is important in characterizing
the quality of petroleum fractions as gasoline blending components and as feeds to catalytic reforming processes, and in characterizing petroleum
fractions and products from catalytic reforming and from thermal and catalytic cracking as blending components for motor and aviation fuels.This
information is also important as a measure of the quality of aviation turbine fuels.
Cat #51600, Silica Gel, Premium Rf, 30 Å, 75-150 μm - Typical Product Specifications
SilicaGel

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Spherical Silica Gel - HPLC, MPLC, Flash & Gravity LC Phases
Spherical vs. Granular
1. Physical Properties
• Spherical has lower pore-volume and high bulk density
Higher loading in the same column volume
• Spherical has high specific resistance.
Low impurity content
• Spherical has high transparency.
Low content of fine particles
2. Particle Size Distribution
• Spherical has lower mean particle size and sharper distribution
Higher efficiency
Lower pressure drop and easier flow rate
• Spherical Shape
Stable chromatography bed
No fine generations
3. Chemical Properties / Impurities
• Spherical has lower impurity levels, especially for calcium
No undesirable tailing of polar compounds
4. Flash Chromatography Test
• Flash chromatography conditions:
Column: 20 x 500 mm glass column
Sample weight: 25 g
1. Benzene 2. Dimethyl phthalate
Mobile phase: n-hexane / ethyl acetate (90-10) w%
Flow rate: 5 cm/min (15.7 ml /min)
Detection: UV 254 nm
Spherical Silica shows higher resolution. The reasons are:
• Smaller particle size.
• Homogeneous packing due to the spherical particle shape.
5a. Adsorption Curve
• A long break through time (point A) indicates higher capacity
• A short mass transfer time (A-B) indicates higher equilibrium
Both silica gels were packed
in 4.6 x 250 mm stainless
steel columns by dry
packing.
Capacity of dimethyl
phthalate (DMP):
Flow rate 2 ml/min
d (solvent) 0.65 g/ml or
1.3 g/min
DMP (1%) 13 mg/min
Capacity (mg) 13 x A
5b. Elution / Desorption Curve
• Short mass transfer time (Short equilibrium) (D-E) indicates lower
solvent consumption
6. Conclusion
When using spherical silica gel you will have
• Increased loading - up to 30% more
• Greater reproducibility of process
• Sharper peaks and cost savings (less solvent consumption & labor)
• Longer bed lifetime (decrease cost of labor and silica consumption)
• Better chromatographic results without switching to costly HPLC
equipment.
• Increased yield with tighter pore symmetry
Spherical Silica Cat. #63700 100 Å, 75-200 μm
Granular Silica Competitor A 100 Å, 60-200 μm
Items Unit Spherical Granular
Surface area (m2
/g) 272 281
Pore volume (ml/g) 0.81 1.14
Bulk density (g/ml) 0.51 0.40
Loss on drying (%) 6.43 1.65
pH (5% suspension) - 7.8 7.5
Specific resistance (Ωcm) 57,100 15,700
Transparency (%) 76 33
Particle Size
d50 (Average) µm 103 143
d10 µm 141 228
d90 µm 80 9
d10/d90 - 1.8 2.5
Chemical Impurities
Na μg/g 298 530
Ca μg/g 90 1100
Fe μg/g 18 60
Column height (mm) 172 235
Retention time (DMP) min 10.61 11.44
k’(DMP) 2.48 3.50
N (DMP) 241 118
Symmetry (DMP) 1.6 2.1
Adsorption Times Unit Spherical Granular
Point A (min) 24.0 19.3
Point B (min) 25.3 21.5
Segment A-B (min) 1.3 2.2
Column Capacity (mg) 312 251
Silica capacity (mg/g) 146 155
Elution Times
Point C (min) 2.3 1.9
Point D (min) 5.8 4.1
Point E (min) 10.5 10.8
Segment C-E (min) 8.2 8.9
Segment D-E (min) 4.7 6.7
C-E: Elution time
D-E: Concentration change time (Mass transfer zone)
SilicaGel

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Catalog No. Particle
Dist.
Porosity pH
Value
Loss on
Drying
Bulk
Density
Surface
Area
Pore
Volume
Compound
Size
72200 5 μm 70 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 500 m2
/g 0.80 ml/g <1,000 Daltons
73200 5 μm 100 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 270 m2
/g 0.80 ml/g <10,000 Daltons
74200 5 μm 150 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 175 m2
/g 0.80 ml/g <10,000 Daltons
75200 5 μm 200 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 130 m2
/g 0.80 ml/g <10,000 Daltons
76200 5 μm 300 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 90 m2
/g 0.80 ml/g <10,000 Daltons
77200 5 μm 500 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 60 m2
/g 0.80 ml/g <50,000 Daltons
78200 5 μm 800 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 45 m2
/g 0.80 ml/g <50,000 Daltons
79200 5 μm 1000 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 30 m2
/g 0.80 ml/g <50,000 Daltons
72300 10 μm 70 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 500 m2
73300 10 μm 100 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 270 m2
/g 0.80 ml/g <10,000 Daltons
74300 10 μm 150 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 175 m2
/g 0.80 ml/g <10,000 Daltons
75300 10 μm 200 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 130 m2
/g 0.80 ml/g <10,000 Daltons
76300 10 μm 300 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 90 m2
/g 0.80 ml/g <10,000 Daltons
77300 10 μm 500 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 60 m2
/g 0.80 ml/g <50,000 Daltons
78300 10 μm 800 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 45 m2
/g 0.80 ml/g <50,000 Daltons
79300 10 μm 1000 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 30 m2
/g 0.80 ml/g <50,000 Daltons
72400 15 μm 70 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 500 m2
73400 15 μm 100 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 270 m2
/g 0.80 ml/g <10,000 Daltons
74400 15 μm 150 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 175 m2
/g 0.80 ml/g <10,000 Daltons
75400 15 μm 200 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 130 m2
/g 0.80 ml/g <10,000 Daltons
76400 15 μm 300 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 90 m2
/g 0.80 ml/g <10,000 Daltons
77400 15 μm 500 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 60 m2
/g 0.80 ml/g <50,000 Daltons
78400 15 μm 800 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 45 m2
/g 0.80 ml/g <50,000 Daltons
79400 15 μm 1000 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 30 m2
/g 0.80 ml/g <50,000 Daltons
72600 20-45 μm 70 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 500 m2
73600 20-45 μm 100 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 270 m2
/g 0.80 ml/g <10,000 Daltons
74600 20-45 μm 150 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 175 m2
/g 0.80 ml/g <10,000 Daltons
75600 20-45 μm 200 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 130 m2
/g 0.80 ml/g <10,000 Daltons
76600 20-45 μm 300 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 90 m2
/g 0.80 ml/g <10,000 Daltons
77600 20-45 μm 500 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 60 m2
/g 0.80 ml/g <50,000 Daltons
78600 20-45 μm 800 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 45 m2
/g 0.80 ml/g <50,000 Daltons
79600 20-45 μm 1000 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 30 m2
/g 0.80 ml/g <50,000 Daltons
62500 40-75 μm 70 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 500 m2
63500 40-75 μm 100 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 270 m2
/g 0.80 ml/g <10,000 Daltons
66500 40-75 μm 300 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 90 m2
/g 0.80 ml/g <10,000 Daltons
67500 40-75 μm 500 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 60 m2
/g 0.80 ml/g <50,000 Daltons
68500 40-75 μm 800 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 45 m2
/g 0.80 ml/g <50,000 Daltons
69500 40-75 μm 1000 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 30 m2
/g 0.80 ml/g <50,000 Daltons
62700 75-200 μm 70 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 500 m2
63700 75-200 μm 100 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 270 m2
/g 0.80 ml/g <10,000 Daltons
66700 75-200 μm 300 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 90 m2
/g 0.80 ml/g <10,000 Daltons
67700 75-200 μm 500 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 60 m2
/g 0.80 ml/g <50,000 Daltons
68700 75-200 μm 800 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 45 m2
/g 0.80 ml/g <50,000 Daltons
69700 75-200 μm 1000 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 30 m2
/g 0.80 ml/g <50,000 Daltons
63900 200-500 μm 100 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 270 m2
/g 0.80 ml/g <10,000 Daltons
64900 200-500 μm 150 Å 6.0 - 8.0 3.0 - 7.0% 0.5 g/ml 175 m2
/g 0.80 ml/g <10,000 Daltons
Spherical Silica Gel - HPLC, MPLC, Flash & Gravity LC Phases
High purity silica gel for normal phase column chromatography.
SilicaGel

12
Flash & Gravity Silica Gel- Spherical
Our flash and gravity grade silicas are ideal for flash chromatography, industrial scale LC, and batch adsorption applications. Sorbtech Flash and
Gravity silicas function through hydrophilic interactions with more polar compounds generally retained longer, making them ideally suited for
the purification of synthetic intermediates, oils, fats, and natural products (vitamins, flavor, fragrances, etc.) Available in 70 Å, 100 Å, 150 Å, 200 Å,
300 Å, 500 Å, 800 Å, and 1000 Å
Flash & Gravity Chromatography Grades - Spherical
HPLC, MPLC Silica Gel- Spherical
Sorbtech HPLC and MPLC Silica Gels are high purity silica gels characterized by low metal ion content and low salt concentrations. Various
particle diameters and pore sizes allow for easy transition from analytical to preparative. These silica gels can be used as the base materials for
functionalization (eg. C18, C8, Amino, etc.) Particle sizes include: 2 μm, 3 μm, 5 μm, 10 μm, 20-45 μm, 40-75 μm, and 75-200 μm with porosities of:
70 Å, 100 Å, 150 Å, 200 Å, 300 Å, 500 Å, 800 Å, and 1000 Å.
HPLC, Prep HPLC, and MPLC Chromatography Grades - Spherical
Tip:
To standardize silica gel from chemist to chemist, lot to lot, run to run, place the amount
of silica gel you are using into a vacuum oven and heat for 30-45 min. at 130° C. Cool
to room temperature, then pack your column. This will eliminate any residual moisture.
Cat. # Product Porosity Particle Dist. Size
10301500-001 Spherical Silica Gel, Premium Pure 100 Å 2 μm 10 g
72200-001 Spherical Silica Gel, Premium Rf™ 70 Å 5 μm 10 g
72600-05 Spherical Silica Gel, Premium Rf™ 70 Å 20-45 μm 500 g
72600-1 Spherical Silica Gel, Premium Rf™ 70 Å 20-45 μm 1 kg
72600-2.5 Spherical Silica Gel, Premium Rf™ 70 Å 20-45 μm 2.5 kg
73600-01 Spherical Silica Gel, Premium Rf™ 100 Å 20-45 μm 100 g
Cat. # Product Porosity Part. Dist. Size
62500-01 Spherical Silica Gel, Premium Rf™ 70 Å 40-75 μm (200 x 400 mesh) 100 g
62500-1 Spherical Silica Gel, Premium Rf™ 70 Å 40-75 μm (200 x 400 mesh) 1 kg
SilicaGel

13
Bonded Phase Silica Gels - Spherical
Sorbtech bonded phases start with Premium Rf™ silica gel as the base material to ensure the greatest purity and performance for all your
laboratory needs. This highest grade silica ensures optimum resolution for even the most difficult separations. Manufactured to strict standards,
Premium Rf™ bonded phases provide uniform porosity and pore volume for high loadability and yield. The exceptional surface coverage of the
alkylsilanes and endcapping techniques provide a surface with the lowest silanol activity. Bonded phases are available in HPLC, MPLC, Flash, and
Gravity grades. (e.g. 2 μm, 3 μm, 5 μm, 10 μm, 20-45 μm, 40-75 μm, and 75-200 μm. Porosities of 70 Å, 100 Å, 150 Å, 200 Å, 300 Å, 500 Å, 800 Å,
and 1000 Å) Packaging sizes available in 10 g, 100 g, 500 g, 1 kg, 2.5 kg, 5 kg and 20 kg.
Phases and their functions
C18 - Octadecyl
Most popular and versatile reversed phase. Very non-polar, hydrophobic interactions with a wide variety of organic compounds.
We offer the following C18 options:
C18 – Standard Bonding with Endcapping. Excellent for routine analysis.
C18AQ - Stable in 100% aqueous Mobile phase systems.
C18AQ2 – Offers 100% aqueous stability designed to match the retention times with leading aqueous C18 brands.
C18DE – Dual Encapping allows for enhanced pH stability 1 – 12.
C8 - Octyl
Similar to C18, but the shorter alkyl chains cause more polar secondary interactions with polar compounds. Less retentive than C18.
We offer two C8 options:
C8 – Standard bonding with endcapping
C8DE – Dual Encapping allows for enhanced pH stability 1 – 12.
Cat. # Product Porosity Part. Dist.
10302026 C8 Premium Pure Spherical Silica Gel, End-capped 100 Å 3 μm
73326 C8 Spherical Silica Gel, Premium Rf, End-capped, Carbon Load: 6-9% 100 Å 10 μm
52526 C8 Silica Gel, Premium Rf™, End-capped, Carbon Load: 8-12% 60 Å 40-75 μm
62526 C8 Spherical Silica Gel, Premium Rf, End-capped, Carbon Load: 8-12% 70 Å 40-75 μm
63527 C8DE Spherical Silica Gel, Premium Rf, Dual End-capped, Carbon Load: 6-9% 100 Å 40-75 μm
72246 C18 Spherical Silica Gel, Premium Rf, End-capped, Carbon Load: 18-22% 70 Å 5 μm
52546M C18 Silica Gel, Premium Rf™, End-capped, Carbon Load: 18-22% 60 Å 40-63 μm
63547 C18DE Spherical Silica Gel, Premium Rf, Dual End-capped Carbon Load: 15-19% 100 Å 40-75 μm
63548 C18 Aqueous, Spherical Silica Gel, Carbon Load: 9.0 - 13.3% 100 Å 40-75 μm
63549 C18 Aqueous II, Spherical Silica Gel, Carbon Load: 9.0 - 13.3% 100 Å 40-75 μm
10353049 C18 Aqueous II, Premium Pure Spherical Silica Gel, End-capped 150 Å 5 μm
66546 C18 Spherical Silica Gel, Premium Rf, End-capped, Carbon Load: 4.5-8.5% 300 Å 40-75 μm
SilicaGel

14
Phenyl
Specific reversed phase for medium size / polar molecules and aromatics, shows a high selectivity for π acceptor / donor compounds.
Cyano
Can be used in both normal and reversed phase. Popular for purification of natural products.
Amino
Will separate a variety of sugars with acetonitrile / water mobile phase.With hexane / ethyl acetate mobile phase, it will function as an alternative to
bare silica during normal phase chromatography and offer a different retention time. In addition, since the surface contains primary amino groups,
this phase can be used as a base for coupling chiral ligands. Also can be used as a weak anion exchanger.
10302055 NH2
Premium Pure Spherical Silica Gel 100 Å 3 μm
10303055 NH2
63554 NH2
Spherical Silica Gel, Carbon Load: 3-5% 100 Å 40-75 μm
10502055 NH2
66555 NH2
Spherical Silica Gel, Premium Rf, Carbon Load: 1-2% 300 Å 40-75 μm
73265 Phenyl Spherical Silica Gel, Premium Rf, End-capped, Carbon Load: 10-14% 100 Å 5 μm
63566 Phenyl Spherical Silica Gel, Premium Rf, End-capped, Carbon Load: 10-14% 100 Å 40-75 μm
63765 Phenyl Spherical Silica Gel, Premium Rf, End-capped, Carbon Load: 10-14% 100 Å 75-200 μm
62560 CN Spherical Silica Gel, Premium Rf, End-capped, Carbon Load: 5-7% 70 Å 40-75 μm
73260 CN Spherical Silica Gel, Premium Rf, End-capped, Carbon Load: 3-5% 100 Å 5 μm
63760 CN Spherical Silica Gel, Premium Rf, End-capped, Carbon Load: 3-5% 100 Å 75-200 μm
C4 - Butyl
More polar than C18 and C8 because it has short alkyl chains and the silica surface is not completely shielded. Used for compounds that are highly
retained on C18 or C8.
C2 - Ethyl
Weakly non-polar. Plasma, urine, and aqueous samples.
52516 C4 Silica Gel, Premium Rf™, End-capped, Carbon Load: 5-7% 60 Å 40-75 μm
SilicaGel

15
HILIC B
Diamine-carboxylic acid type, ideal for nucleic acids, organic acids, etc.
HILIC A
Butyl-amine-carboxylic acid type, ideal for amino acids & small peptides, etc.
SH (Thiol)
Scavenger of electrophiles (eg. alkyl, benzyl, and allyl halides, acid chlorides, isocyanates). Metal scavenger (eg. Pd, Pt, Cu, Hg, Ag, Pb).
SCX
Propyl Sulfonic Acid (HSO3
), strong cation exchanger.
WCX
Carboxylic Acid (COOH), weak cation exchanger.
Diol
Particularly used for aqueous GPC applications. Diol can also be used for normal phase. DIOL can function as an alternative stationary phase during
normal phase chromatography and offer a different retention time than either bare silica gel or amino bonded silica gel due to its neutral charge.
63590 HILIC A Spherical Silica Gel, Premium Rf 100 Å 40-75 μm
63591 HILIC B Spherical Silica Gel, Premium Rf 100 Å 40-75 μm
63785 Thiol (Scavenger) Spherical Silica Gel, Premium Rf 100 Å 75-200 μm
63580 SCX (strong cation exchanger) Spherical Silica Gel, Premium Rf 100 Å 40-75 μm
63577 WCX (weak cation exchanger) Spherical Silica Gel, Premium Rf 100 Å 40-75 μm
10302070 Diol Premium Pure Spherical Silica Gel 100 Å 3 μm
10303070 Diol Premium Pure Spherical Silica Gel 100 Å 5 μm
63570 Diol Spherical Silica Gel, Premium Rf 100 Å 40-75 μm
63770 Diol Spherical Silica Gel, Premium Rf 100 Å 75-200 μm
SilicaGel

16
Dry Column Chromatography - Go Green!
DryColumChromatography(DCC)isafast,easy,andefficientmethodforseparating
and/or purifying industrial quantities of compounds. DCC links analytical thin
layer chromatography (TLC) with preparative column chromatography (CC). Like
TLC, DCC is a “non-elution” method utilizing Nylon Foil Tubing as a support and is
available with UV-254 indicator.
In TLC solvent flow is by capillary action going against gravity whereas in DCC,
solvent flow is by gravity. Columns of any size may be filled in DCC to separate or
clean up very large quantities of materials.
Both silica and alumina sorbents are available for DCC. Nylon Foil Tubing is solvent
resistant and available in 1”- 6”diameters.
Go Green with less solvent use.
DCC Experiment
DCC is a“non-elution”method of column chromatography, meaning, the separated substances remain in the column at the end of
the chromatographic process. The use of nylon foil tubing allows for the removal of these separated substances by simply cutting
the tubing into segments.
Preparation of the column
The nylon foil tubing comes in a roll of various widths in lengths of 100 ft. See table on next page.
Cut the tubing to the desired length. Allow an extra 10 cm for sealing the end. Close one end of
the foil by sealing in the flame of a gas lighter, folding and stapling, tying up, or any other suitable
method. To remove the folds of the foil, fill one side of the tubing with very hot water, rinse with
acetone, and dry with hot air. Insert a pad of cotton or glass wool into the closed end of the tubing
and pierce several times with a needle to allow air to escape while the solvent migrates through the
column.
Filling the column
Carefully fill the tubing, adding small quantities at a time, with Silica DCC, Alumina DCC, or sorbent
that has been conditioned with the solvent (mixture). After each small quantity filled, the column
should be gently tapped on a hard surface or compacted with a vibrator. Upon filling completion,
the column will be so rigid that it will hold itself upright and may be fixed with a simple laboratory
clamp to a stand.
Preparation of the sample
A few milliliters of a sample solution are added to about 1 g of the sorbent. (A larger amount of
sorbent may be added to a larger sample solution with the practical ratio of the mixture to be
separated (without solvent) to sorbent is normally about 1:5 (w/w)). The solvent is then evaporated
using a rotation evaporator until sample and sorbent form a free flowing powder.
Product pH Activity* Fluorescent Indicator Bulk Density approx. Surface Area approx.
Silica for DCC 7 III 0.5% 0.5 g/ml 500-600 m2
/g
Alumina for DCC 7 III 0.5% 0.8 g/ml 200 m2
/g
*Activity according to Brockmann
DCC

17
14400-05 Silica for DCC, w/UV254, 60 Å 63-200 μm (70 x 230 mesh) 500 g
14400-3 Silica for DCC, w/UV254, 60 Å 63-200 μm (70 x 230 mesh) 3 kg
14400-25 Silica for DCC, w/UV254, 60 Å 63-200 μm (70 x 230 mesh) 25 kg
19500-05 Alumina for DCC, w/UV254 50-200 μm (70 x 270 mesh) 500 g
19500-5 Alumina for DCC, w/UV254 50-200 μm (70 x 270 mesh) 5 kg
19500-50 Alumina for DCC, w/UV254 50-200 μm (70 x 270 mesh) 50 kg
NT-1 Nylon Tubing for DCC - 1”x 100’
NT-1.5 Nylon Tubing for DCC - 1.5”x 100’
NT-2.5 Nylon Tubing for DCC - 2.5”x 100’
Charging the column
The adsorbate of the sample is applied as a level layer on the top of
the column. This layer is covered with about 2 cm of pure sorbent. If a
concentrated sample solution is to be applied, care should be taken to
achieve a starting zone, as narrow as possible. Applying liquid sample
causes more uneven zones than applying the sample as an adsorbate.
After penetration of the solution into the top of the column filling,
about 2 cm of pure sorbent will be applied.
Development of the column
The solvent (mixture) that formed the best results in the preliminary
TLC experiments is carefully added to the top of the column by means
of a separatory funnel. The solvent should migrate slowly and steadily
through the column. A constant liquid head of about 1 - 2 cm gives
the best results. A constant liquid head is attained by placing the end
of the stem of the topside closed separatory funnel about 1 cm over
the top of the column. Depending on the length of the column, the
viscosity of the solvent (mixture), and the height of the liquid head,
the development of the column should be finished within 15 to 30
minutes. As with TLC, the components of the initial sample mixture
should be separated from each other on the column. The solvent
volume applied to the top of the column shouldn’t exceed the amount
which is necessary to fill the void fraction of the adsorbent bed.
Recovery of the separated compounds
After completing development, the column is immediately placed flat
on an appropriate cutting surface. The separated zones are marked on
the column, using a UV light if necessary. The column is then cut into
sections with a sharp knife. The column may be cut into small slices
without sorbent crumbling. The cut slices are placed into Büchner
funnels and extracted and processed by appropriate methods.
DCC

18
Alumina
Alumina
Sorbent Technologies’ aluminas for column chromatography are the best
available. A stringent manufacturing process yields standardized aluminas
which are measured according to Brockmann methods.This ensures precise
specifications and lot to lot reproducibility.
Our aluminas are ideal for natural product extraction, purification & de-
colorization, heavy metal removal, cleanup, and more.
We offer the broadest selection of standardized aluminas with defined pH
values (acid, base, neutral), specific activity levels (Super I, Activity I, II, III, IV,
V) and numerous particle distributions (MPLC, Flash, Gravity).
These aluminas can be adjusted to lower activity by the addition of polar
media, preferably water. See Deactivation Procedure on next page.
Typical Applications for Aluminas
Acidic
Super I
Food dyestuffs
Determination of morphine
Determination of biphenyl
Standard Activity I
Plant extraction
Fatty acids
Plant waxes
Azobenzene
Neutral
Super I
Ketosteroids
Glycosides
Vitamins
Removal of pyrogens
Dehydration of organic solvents
Alkaloids
Standard Activity I
Antibiotics
Essential oils
Plant extraction
Enzymes
Glycosides
Hormones
Diesel exhaust
Pharmaceutical compounds
Fly agaric
Basic
Super I
Purification of organic solvents
Synthetic bases
Dehydration of ethers
Removal of pyrogens
Natural and synthetic dyestuffs
Hydrocarbons
Standard Activity I
Alkaloids
Essential oils
Plant extraction
Purification of organic solvents
Insecticides
Clarification of fatty oils
Removal of alcohol from chloroform
Alumina

19
Deactivation of Alumina
Deactivation allows the chromatographer the flexibility and freedom to deactivate alumina to the precise activity level required for the specific
application. Alumina may be deactivated by the addition of polar media, preferably water, but also alcohols such as glycol or glycerol can be used.
Sorbent Technologies’ standardized aluminas are based on a deactivation scale. Alumina Super I is standardized to such a high degree that all
three surface types of Aluminas, (acid, basic, neutral) show an identical initial activity, and when deactivated with equal amounts of deactivator
(e.g. water), they reach the same lower activity status. The percent of water addition is based upon weight/weight relationships. Activity level is
according to Brockmann.
Deactivation Procedure:
1. Weigh a known quantity of alumina and place into a tightly sealed vessel
2. Add the required amount of water (by weight or volume)
3. Shake the mixture in the closed vessel until all lumps disappear
4. Allow the mixture to cool and equilibrate (preferably overnight)
5. The container should remain tightly closed to retain activity level and can be stored for later use
Activity Super I I II III IV V Activity Level According to Brockmann
Super I 0 1 4 7 10 19 % water to add
Std. Act. I N/A 0 3 6 10 15 % water to add
The amount of water needed for deactivation can be read off the graph below:
Alumina pH
approx.
Activity LOI
approx.
Particle
Dist.
Water Sol.
Matter
approx
Bulk
Density
approx.
Surface Area
approx.
Super I - Acidic 4.5 Super I 1.3% 50-200 μm 0.1% 0.8 g/ml 200 m2
/g
Super I - Neutral 7.5 Super I 1% 50-200 μm 0.1% 0.8 g/ml 200 m2
/g
Super I - Basic 10 Super I 1% 50-200 μm 0.1% 0.8 g/ml 200 m2
/g
Standard Act. I - Acidic 4.5 Act. I 1.7% 50-200 μm 0.1% 0.8 g/ml 150 m2
/g
Standard Act. I - Neutral 7.5 Act. I 1.7% 50-200 μm 0.1% 0.8 g/ml 150 m2
/g
Standard Act. I - Basic 10 Act. I 1.7% 50-200 μm 0.1% 0.8 g/ml 150 m2
/g
Act. II-III 10 Act. II-III 50-200 μm 0.8 g/ml
Alumina N for Flash Chromatography 7.4 32-63 μm 0.2% 0.8 g/ml 200 m2
/g
Alumina B - Super I for Dioxin Analysis 10 0.7% 50-200 μm 0.1% 0.8 g/ml 200 m2
/g
Alumina R 4.3 50-200 μm 0.8 g/ml
SG Sudan
Alumina

20
15100-1 Alumina, Super I - Acidic 50-200 μm (70 x 270 mesh) 1 kg
15100-5 Alumina, Super I - Acidic 50-200 μm (70 x 270 mesh) 5 kg
15130-1 Alumina, Super I - Neutral 50-200 μm (70 x 270 mesh) 1 kg
15130-5 Alumina, Super I - Neutral 50-200 μm (70 x 270 mesh) 5 kg
15160-1 Alumina, Super I - Basic 50-200 μm (70 x 270 mesh) 1 kg
15160-5 Alumina, Super I - Basic 50-200 μm (70 x 270 mesh) 5 kg
Alumina, Super I
Alumina Activity Super I is designed for the separation of polar samples in non-solvent systems and the purification and dehydration of solvents for
HPLC and other analytical techniques. In a non-polar environment, Alumina Activity Super I shows sorption capacity approximately twice as high
as that of Alumina Standard Activity I. Its initial activity (<1% moisture) is kept in a very narrow range and its deactivation is extremely constant.
15600-1 Alumina, Standard Act. I - Acidic 50-200 μm (70 x 270 mesh) 1 kg
15740-1 Alumina, Standard Act. I - Neutral 50-200 μm (70 x 270 mesh) 1 kg
15640-1 Alumina, Standard Act. I - Basic 50-200 μm (70 x 270 mesh) 1 kg
Alumina, Standard Act. I
Alumina, Standard Activity I is similar to the Alumina, Activity Super I in that it is also standardized. The starting activity level is about half as
high and can be deactivated according to the deactivation table on the previous page. Standardized manufacturing processes provide a specific
Brockmann dryness level for lot to lot reproducibility and precise deactivation possibilities. Between 1 - 3% moisture.
15300-1 Alumina, Activity II-III 50-200 μm (70 x 270 mesh) 1 kg
Alumina, Activity II-III
Alumina, Act. II-III is an economical adsorbent of medium activity. Alumina, Act. II-III is often used when activated charcoal cannot be used due to
its organic nature or where the cation exchange properties of basic alumina are desired. It is suitable for the development of separation methods
and technical separations where a custom-made adsorbent is not available. Between 3 - 6% moisture.
16050-1 Alumina, Neutral for“Flash”Chromatography 32-63 μm (230 x 450 mesh) 1 kg
19600-05 Alumina B - Super I for Dioxin Analysis 50-200 μm (70 x 270 mesh) 500 g
19650-05 Alumina R for the Technique of Isotopes 50-200 μm (70 x 270 mesh) 500 g
Other Aluminas
Alumina, Neutral for “Flash” Chromatography has a finer particle distribution than the Super I and Act. I aluminas for sharper separations.
Other finer cuts are also available.
Alumina B – Super I for Dioxin Analysis was specially developed to meet the high recovery level required for the analysis of polychlorinated
dibenzodioxins and dibenzofurans according to VDI-Regulation 3499, part I.
Alumina R is an acidic alumina developed for use in isotope chemistry. It is used to produce technetium generators or technetium cows. Another
example of its application is the determination of catecholamines.
Alumina

21
Solvent
Alumina,
Acid,
Act. I
Cat.# 15600
Alumina,
Neutral,
Act. I
Cat.# 15740
Alumina,
Basic,
Act. I
Cat.# 15640
Silica Gel,
Active, 60 Å,
63-200 μm
Cat.# 10840
Solvent
Yield
(ml)
50% Trans.
at nm
(4 cm
cuvette)
n-Pentane 40 g 250 217
40 g 55 217
n-Hexane 40 g 250 218
40 g 50
Cyclohexane 40 g 700 233
40 g 80 234
n-Heptane 40 g 500 224
40 g 70 232
Isooctane 40 g 40 g 250 226
40 g 1600 224
40 g 255 255
40 g 255 255
Carbon
tetrachloride
40 g 80 280
40 g 4000 276
Chloroform DAB6 40 g 340
40 g 100
Nitromethane 40 g 40 402
Dimethy lsulfoxide 40 g 170 326
Pyridine 40 g 30 321
40 g 35 332
40 g 30
20 g 20 g 25 316
Alumina - Solvent Purification
Solvent Purification Methods:
UV transparent solvents are essential for spectroscopy below 300 nm. Classical methods of purifying solvents for UV spectroscopy are very time-
consuming and complex. This is not the case when using adsorption filtration. UV transparent solvents can be quickly and easily prepared from
solvents of ordinary analytical purity. Moreover, they are less sensitive to air and thus more stable.
Purification of Hydrocarbon Solvents
• A sample of 300 ml analytical grade n-hexane or pre-purified (by shaking with sulfuric acid to remove alkenes
or olefines) technical grade cyclohexane is simply filtered without suction through a column (25 mm I.D. and 250
mm height) filled with 150 g Alumina Basic, Act. I (Cat.# 15640). The filtrate is anhydrous and can immediately be
used.
• Technical grade cyclohexane, that is not pre-purified, can easily be purified by filtering through a combined Silica
Gel, Active, 60 Å, 63-200 μm / Alumina Basic, Act. I column. A column (25 mm I.D. and 400 mm height) is first filled
with 100 g Alumina Basic, Act. I (Cat.# 15640) and on top of this,100 g of Silica Gel, Active, 60 Å, 63-200 μm (Cat.#
10840) is added. Then the crude cyclohexane is dripped through. Purified cyclohexane is anhydrous.
Purification of Organic Solvents
Depending on the kind of impurities, which depends on the origin and the nature of the solvent, the solvent can
be filtered through a column filled with Silica Gel, Active, 60 Å, 63-200 μm, Alumina (Acid, Neutral or Basic), Act. I,
or a combination of the two with the alumina section of the column above the silica gel section of the column.The
following table gives recommendations on the amount and type of adsorbent to be used as well as the solvent
yield and obtained purity.
Silica Gel,
Active,
60 Å 63-200 μm
Alumina,
Basic,
Act. I, 50-200 μm
Silica Gel,
Active,
60 Å, 63-200 μm
Alumina, (Acid,
Neutral or Basic)
Act. I, 50-200 μm
Silica Gel,
Active,
60 Å, 63-200 μm
Alumina, (Acid,
Neutral or Basic)
Act. I, 50-200 μm
+
G. Hesse and H. Schildknecht, Angew. Chem. 67, 737 (1955) G. Hesse, B. P. Engelbrecht, H. Engelbrecht and S. Nitsch, Z. Anal. Chem. 241, 91 (1968)
Alumina

22
Activated Alumina for Large Scale Purification
Alumina for Large Scale Purification is a granular activated alumina that has a high surface area and a large pore volume. Because of these physical
characteristics it has a wide variety of applications. Powders can be manufactured to meet specific customer needs and packaged in bulk super-
sack sizes.
Alumina for Large Scale Purification can adsorb a great number of pollutants from liquids and vapors. Flocculation adsorption can also occur
depending on the pH-value. The degree of adsorption depends on the polarity of the substance to be adsorbed; polar substances are bound
stronger than non-polar ones. In mixtures of substances with differing polarity, a chromatographic separation will take place.
Typical Applications include:
• Drying and purification of organic liquids and gases
• Elimination of pollutants from circulating reaction mixtures
• Elimination of system-damaging substances in circulation loops
• Selective purification of raw materials
• Concentration of highly diluted product or by-product solutions for recycling
• Adsorption of heavy metals (soluble compounds) in underground water and drinking water
- Fluorides - Phosphates - Arsenic acids - Humic acids
• Purification of processing liquids, industrial water, and waste water
- Fluorides - Phosphates - Arsenic acids
- Acidic phenols - Amines - Ionic detergents
- Lignosulfonic acids - Chlorine lignine - Vapor condensates
- Organic acids - Acidic / basic dyes - Dyestuffs waste water
- Bleach waste water (pulp industry)
• Purification and drying of exhaust air and gases
- Fluorine - Hydrogen fluoride - Odors (in testing phase)
Procedure:
Reduction of waste levels with Alumina for Large Scale Purification is possible in every kind of adsorption reactor. Sufficient contact time between
the adsorbent and the medium to be purified must be ensured. Process conditions must be defined individually.
When using common fixed-bed reactors, approximate contact times of 0.5 - 0.75 hours and filtration rates of 1-2 m/hr can be used in the installation
design. Optimal adsorption levels of by-products and waste products can thus be attained.
Regeneration:
In most application areas, regeneration of saturated alumina is not only desirable, but also possible. When designing a continuous adsorption
plant, the reaction design should reflect the cycling of the saturated adsorbent in sequence with the regeneration phase. The total adsorption
capacity should be calculated to assure optimal adsorption. The design of the regeneration procedure depends on both the nature of the adsorbed
substances and on their recyclability.
Thermal treatment (up to a maximum of 600° C) can remove organic adsorbed impurities during regeneration without losing alumina activity. By
adjusting the pH, inorganic adsorbed impurities, such as phosphates or fluorides, can be eluted chemically.
After recycling by one of the regeneration processes, the alumina is ready for use again. Based on previous experience, each regenerating cycle
results in a 1-3% loss of alumina due to abrasion. This loss must be compensated for when restarting each individual reactor.
Typical Technical Data
Specific surface area (BET): 230-300 m2
/g
Loss on ignition: ~ 9%
Pore volume: ~ 0.35 cm3
/g
Chemical analysis: Al2
O3 ~ 90%
Na2
O total 0.3 - 0.5%
CaO 0.01 - 0.03%
Fe2
O3
0.01 - 0.03%
SiO2
0.01 - 0.03%
TiO2
~ 0.005%
85800-100 Activated Alumina for Water/Process Liquids Clean-up < 0.5 mm 100 kg
85801-100 Activated Alumina for Water/Process Liquids Clean-up 0.3 - 1 mm 100 kg
85813-100 Activated Alumina for Purification 1 - 3 mm 100 kg
85825-100 Activated Alumina for Purification 2 - 5 mm 100 kg
For more selections and sizes, visit our website or contact your personal Product Specialist
Alumina

23
Gases
Air
Ammonia
CO2
Chlorine
Ethane
Ethylene
Furnace gas
Helium
Hydrogen
Hydrogen
chloride
Hydrogen sulfide
Methane
Natural gas
Nitrogen
Oxygen
Propane
Propylene
Refrigerants
Sulfur dioxide
Liquids
Benzene
Butane
Butene
Butyl acetate
Carbon tetrachloride
Chlorobenzene
Cyclohexane
Ethyl acetate
Gasolines
Heptane
Hexane
Hydraulic oils
Jet fuel
Kerosene
Lubricating oils
Naptha
Nitrobenzene
Pentane
Pipeline products
Propane
Propylene
Refrigerants
Styrene
Toluene
Transformer oil
Vegetable oil
Xylene
Alumina Spheres
Sorbtech’s activated alumina spheres are very porous. They have a high surface area, which allows adsorption of gases and liquids, and do not
swell. They will not soften or disintegrate when immersed in liquids. When used to adsorb water, they act as an efficient desiccant. Molecules
with the highest polarity are preferentially adsorbed. In the absence of irreversible flowing, they may be regenerated to their original adsorption
efficiency by heating to a temperature between 350-600° F (177-316° C). For the most efficient subsequent adsorption, high temperature, low
pressure and dry inert gas regeneration is recommended.
Benefits:
1. Uniform ball size
• Especially important in high pressure gas
dehydration for reduction of pressure drop
• Minimizes channeling
• Yields higher efficiencies
2. High Crush Strength
• Allows rapid pneumatic loading
• Enables use of tall towers
• Effective for the dehydration of acid
containing gases and liquids, such as CO2
• Longer operating life
3. Low Abrasion
• Ensures less dusting during transport,
loading, and service life
• Reduces downstream valve and filter
plugging
4. High Adsorptive Capacity
• High surface area
• Tailored pore distribution
• Achieve an ultra low H2
O effluent
Available in 1 kg, 5 kg, 25 kg, 50 kg, 100 kg and Bulk Super Sack packaging
Adsorption Isotherm
for Alumina Spheres @25° C
% Relative Humidity
Wt.%H20Adsorbed
Product Applications:
Process stream purification
Removal of highly polar compounds such as TBC, alcohols, ethers, etc.
Acid removal
Our alumina spheres can remove the acids formed from the degradation of transformer oils, lubricating oils, and refrigerants. Also, they remove
the residual halides and water during the manufacture of chlorinated and/or fluorinated hydrocarbons, yielding a non-corrosive product.
Drying
Nearly all gases and liquids can be dried with alumina. Water removal is often necessary for efficient processing, storage, transportation or usage
of fluids. For example, water can form hydrates with many compounds including hydrocarbons, simulating ice that may plug equipment.
Substances dried with alumina spheres:
Cat. #
Sphere
Size
(English)
Sphere
Size
(Metric)
Surface
Area
Total pore
volume
Packed bulk
density
Crush
strength
Abrasion
loss wt%
Static
sorption at
11% RH
Static
sorption at
58% RH
Static
sorption at
97% RH
92000 7 x 14
Tyler
Mesh
2.0 mm 360 m2
/g 0.5 cc/g 48 lb/ft3
(769 kg/m3
)
11 lb
(5 kg)
0.1 8 22 42
92010 1/8” 3.2 mm 355 m2
/g 0.5 cc/g 48 lb/ft3
(769 kg/m3
)
30 lb
(14 kg)
0.1 8 22 42
92020 3/16” 4.8 mm 340 m2
/g 0.5 cc/g 48 lb/ft3
(769 kg/m3
)
55 lb
(25 kg)
0.1 7 21 40
92030 1/4” 6.4 mm 325 m2
/g 0.5 cc/g 48 lb/ft3
(769 kg/m3
)
70 lb
(32 kg)
0.1 7 19 38
Alumina

24
26500-025 Polyamide for TLC 2-20 μm 250 g
26500-1 Polyamide for TLC 2-20 μm 1 kg
26520-025 Polyamide for Flash Chromatography <70 μm 250 g
26520-1 Polyamide for Flash Chromatography <70 μm 1 kg
26530-025 Polyamide for Classic Column Chromatography 50-160 μm 250 g
26530-1 Polyamide For Classic Column Chromatography 50-160 μm 1 kg
26530-5 Polyamide For Classic Column Chromatography 50-160 μm 5 kg
26540-1 Polyamide for Process 100-300 μm 1 kg
Specialty Adsorbents
Polyamide
Polyamide is well suited for the separation of natural substances with phenolic and
polyphenolic groups.
The chromatography of phenolic compounds on polyamide has been frequently applied for
the isolation and structure determination of different natural products. Carboxylic acids are
also bonded to polyamide via hydrogen bonds. The affinity for an aliphatic monocarboxylic
acid is low. Dicarboxylic acids and aromatic acids are retained stronger, especially if the
aromatic part of the molecule is larger.
Strong affinity for polyamide is found with numerous aromatic nitro compounds. The
interaction between the nitro compound and polyamide corresponds to a reaction of a Lewis
acid with a base. This is why separations of aromatic nitro compounds on polyamide can be
considered as ion exchange. For this reason elution in sharp bands requires an eluent with
good buffering properties. This principle has been successfully applied for the separation of
dinitrophenylamino acids.
Quinones are irreversibly bonded to the polyamide due to the adsorbent.
Polyamides exhibit constant selectivities towards:
• Phenols, aromatic nitro and amino compounds • Chalcones, quinones, flavones
• Carbonic acids and their amides • Anthraquinones
• DNP-amino acids • Sulfonic acids and their amides
09410-05 Florisil® - A Grade, Activated at 650° C 16 x 30 mesh 500 g
09410-20 Florisil® - A Grade, Activated at 650° C 16 x 30 mesh 20 kg
09435-05 Florisil® - A Grade, Activated at 650° C -200 mesh 500 g
09435-20 Florisil® - A Grade, Activated at 650° C -200 mesh 20 kg
09500-05 Florisil® - Pesticide Residue Grade 60 x 100 mesh 500 g
09500-20 Florisil® - Pesticide Residue Grade 60 x 100 mesh 20 kg
Bulk sizes are available
Florisil®
Florisil® is a very hard granular magnesia silica gel.
MgO 15.5 ± 0.5% SiO2
84.0 ± 0.5% Na2
SO4
≤ 1.0%
Applications include clean-up of pesticide residues, separation of chlorinated pesticides, isolation of steroids, isolation of sex hormones, isolation
of antibiotics, and separation of lipids.
SpecialtyAdsorbents

25
Alumina Ceramic Beads
• Composition: Alkali alumino silicate ceramic
• Shape: Solid spheres
• Color: White
• High strength: Crush strength > 4,200 kg/cm2
(> 60,000 psi)
Glass Beads
For use in column affinity chromatography for isolation and study of glycoprotein factors promoting attachment and spreading of cells in culture.
• Composition: Soda-lime-borosilicate glass
• Shape: Hollow spheres with thin walls
• Color: White, powdery
• Free flowing
• High strength
Silica Desiccants
Sorbtech Silica Desiccants are non-corrosive, non-toxic, and chemically inert. It is a highly porous form of silica,
with an extremely large internal surface area and an affinity towards water and other polar compounds. Will not
change its size or shape. Even when the silica gel is water saturated, it remains free-flowing.
Silica desiccants are available with blue (cobalt chloride) or yellow (phenol phthalein) indicator. Blue indicator
spheres turn pink upon reaching moisture capacity and yellow spheres turn blue-green. The yellow indicator
spheres are non-hazardous, making them perfect for pharmaceutical and food products.
SpecialtyAdsorbents
Cat # Description Size Qty.
22000-1 Silica Gel Desiccant Spheres 1-3 mm 1 kg
22102-1 Silica Gel Desiccant Spheres w/ 20% Blue Indicator 1-3 mm 1 kg
23210-1 Silica Gel Desiccant Spheres w/100% Yellow Indicator 2-5 mm 1 kg
Cat # Description Density Qty.
93015-01 Glass Beads, 9-24 μm (15 μm mean) 0.60 g/cc 100 g
Cat # Description Surface Area Density Qty.
93903-01 Alumina Ceramic Beads, 1-12 μm (3 μm mean) 5 m2
/g 1.5 g/cc 100 g
/g 1.5 g/cc 100 g
/g 1.5 g/cc 100 g

26
Polymeric Resins
Mitsubishi Polymer Resins - Ion Exchange, Purification
We offer an amazing selection of Polymer Resins from Mitsubishi® (PSDVB). For over 60 years
Mitsubishihasbeenmanufacturingionexchangeresinswithexcellentperformance,superiorphysical
and chemical properties, and lot-to-lot reproducibility for lab and industrial separation, extraction
and purification applications. Polymer resins are a series of products based on ion-exchange resin
manufacturing technology and are specifically designed as a solid extractant. Synthetic adsorbents
have large surface areas and fine pore structures inside the particle similar to activated carbon.
Because of this characteristic, these resins can effectively adsorb organic compounds from aqueous
solutions. Polymer resin adsorbents are stable in either acidic or alkaline solutions as well as organic
solvents. These resins can also be easily regenerated for repeat use, increasing product life. Extraction
processes with synthetic adsorbents reduce solvent usage and provide safer operations compared to
conventional solvent extraction techniques. These resins are pH stable from 1-14, available from 10
μm to 1000 μm particle sizes, wide array of pore sizes, and have a long lifetime.
Packaging sizes from 10 g to 200 L.
Synthetic Adsorbents
Aromatic Type: Diaion® - HP20, HP20SS, SP20SS, Sepabeads® - SP825
Food Grade Aromatic Type: Sepabeads® - SP70, SP700, SP710
Modified Aromatic Type: Sepabeads® - SP207, SP207SS
Methacrylic Type: Diaion® HP2MG, SP2MGS
HP20 synthetic adsorbent resin is a macroporous styrenic polymeric bead type resin designed for adsorption/desorption process scale applications.
Its matrix provides an aromatic non-polar surface with excellent selectivity for hydrophobic areas of molecules, including biomolecules like
antibiotics via low energy Van der Waal’s interactions. It is remarkable for its wide pore polymeric structure which provides excellent broad
spectrum adsorption characteristics.
HP20SS and SP20SS are smaller size versions of HP20.The wide pore polymer matrix provides excellent kinetics and capacity for small biomolecules
of both preparative and process scale. They offer a nice balance of pressure flow characteristics and true chromatographic fractionation and have
also been successfully applied in simulated moving bed (SMB) applications for a variety of small biomolecules. They often compete with bonded
silica supports for preparative and industrial applications offering a longer lifetime.
The Sepabeads® SP825 resin provides a smaller more uniform pore size distribution and higher surface area as compared to Diaion® HP20. The
increase in surface area is approximately two times that of traditional adsorbents, and nearly matches the surface area of activated carbon. In
many applications, this means twice the working capacity for the same volume of resin. They are recommended for industrial chemical process
applications, bio/pharmaceutical applications such as desalting and extraction, and waste treatment.
SP70, SP700, and SP710 are premium grade resins designed for the debittering of juices and related food products. These high surface area resins
offer excellent kinetics and high capacity for naringin (and other bittering agents). They meet the compositional requirements for Secondary
Direct Food Additives (21 CFR Section 173.65). SP70 and S710 are provided pre-conditioned to meet the extractive requirements as of the date
of manufacture.
SP207 resin is a macroporous chemically modified brominated styrenic polymeric type resin. The bromination of the aromatic ring provides
increased hydrophobicity, and consequently, increased selectivity for hydrophobic molecules versus conventional styrene/DVB synthetic
adsorbents like Diaion® HP20 resin. In addition, the bromination increases the resin density which allows for settling in fermentation broths, and
for usage in upflow fluidized beds.
SP207SS is a small size version of modified aromatic type Sepabeads® SP207. SP207SS is used for applied to reversed phase chromatography.
The brominated polymeric matrix provides unique selectivity, full pH operating range and long operating life versus conventional bonded silica
packing materials used in preparative and industrial applications.
HP2MG is a macroporous methacrylate polymeric resin. It has no aromatic character. HP2MG grades have different selectivity than the classic
Diaion® HP20. HP20 is recommended for broad spectrum adsorption and desorption of small and large molecules, long chain aliphatic molecules,
de-colorization and desalting applications, and should be evaluated as an alternate resin matrix to the styrenic based adsorbents.
SP2MGS is a small and uniform particle size version of the methacrylate type Diaion® HP2MG. SP2MGS gives higher dynamic capacity than HP2MG
and shows strong retention and unique selectivity in normal phase chromatography.
PolymericResins

27
Product Matrix Ceph C Capacity* Water Retention Shipping Density Particle Size Dist.
HP20 Highly Porous (styrene, DVB) ~38 g/L 55%-65% ~680 g/L On 250 μm: 90% min
SP825L Highly Porous (styrene, DVB) ~80 g/L 52%-62% ~685 g/L On 250 μm: 90% min
SP70 Highly Porous (styrene, DVB) - 55%-65% ~685 g/L On 250 μm: 90% min
SP207 (Brominated styrene-DVB) ~120 g/L 43%-53% ~790 g/L On 250 μm: 90% min
HP2MG (Methacrylate) <10 g/L 55%-65% ~720 g/L On 300 μm: 90% min
HP20SS Highly Porous (styrene, DVB) - 55%-67% ~670 g/L >150 μm: 15% max
63-50 μm: 70% min
<63 μm: 20% max
SP20SS Highly Porous (styrene, DVB) - 55%-65% ~660 g/L >75 μm: 30% max
63-75 μm: 55% min
<63 μm: 15% max
SP207SS (Brominated styrene-DVB) - 43%-53% ~780 g/L >150 μm: 15% max
63-150 μm: 70% min
<63 μm: 20% max
SP2MGS (Methacrylate) - 61%-69% ~720 g/L >220 μm: 1% max
120-160 μm: 85% min
<90 μm: 1% max
Product Effective
Size
Surface Area Pore Volume Pore
Radius
Specific
Gravity
Swelling Water
to Toluene
Operation
Temperatures
HP20 0.25 mm min ~590 m²/dry-g ~1.3 ml/gram 290 Å 1.01 30% 130° C max
SP825L 0.25 mm min ~930 m²/dry-g ~1.4 ml/gram 70 Å 1.01 20% 130° C max
SP70 0.25 mm min ~870 m²/dry-g ~1.5 ml/gram 70 Å 1.01 24% 130° C max
SP700 0.25 mm min ~1200 m²/dry-g ~2.3 ml/gram 90 Å 1.01 7 130° C max
SP710 0.25 mm min ~900 m²/dry-g - 90 Å 1.01 - 130° C max
SP207 0.25 mm min ~600 m²/dry-g ~1.0 ml/gram 110 Å 1.19 20 130° C max
HP2MG 0.30 mm min ~570 m²/dry-g ~1.3 ml/gram 240 Å 1.09 5 130° C max
HP20SS - ~560 m²/dry-g ~1.2 ml/gram ~290 Å - - 130° C max
SP20SS - ~560 m²/dry-g ~1.2 ml/gram ~290 Å - - 130° C max
SP207SS - ~590 m²/dry-g ~1.0 ml/gram ~110 Å - - 130° C max
SP2MGS - ~520 m²/dry-g ~1.3 ml/gram ~230 Å - - -
Adsorption conditions
Influent Solution: 4300 ppm C.P.
SV: 4 BTP: 2150 ppm
Rinsing Volume: 2BV/HP20
Elution Condition: SV 4
Adsorbent: 20 ml (Diaion® HP20)
30% Aqueous Isopropanol Solution
Purification of Antibiotics
Non-ionic synthetic adsorbents are able to hydrophobically
adsorb antibiotics which do not have dissociating groups.
In addition, they are also able to adsorb those with carboxyl
groups, because under the weakly acid pH conditions
in which antibiotics are stable, the carboxyl group is
hardly dissociated. Moreover, their adsorption is usually
strongest under weakly acid conditions and, therefore these
adsorbents find wide application.
The figure to the right shows the elution behavior of
cephalosporin C. While operating conditions will not be
exactly identical, there are many cases where a substance
which can be separated by C18 Silica Gel may also be
separated by the use of a synthetic adsorbent, such as
Diaion® HP20 in this case.
PolymericResins

28
Bioseparation: Wide Pore Hydrophilic Anion Exchange Resins
QA Type: MCI GEL® CQA35S, CQA35P
DEAE Type: MCI GEL® CQA31S, CQA31P
Diethyl Amine Type: Sepabeads® FP-DA13
The CQ grade resins are based on a highly crosslinked polymethacrylic matrix, which is further hydroxylated for added hydrophilicity. These
matrices are functionalized with either a quaternary amine (trimethyl amine) or a diethyl-aminoethyl to form strong basic and weak basic anion
exchangers respectively with a chloride counterion. By varying the bead size of these resins, the chromatographer can control the efficiency and
the resolution in the separation of anionic proteins, peptides, and polynucleotides (separation ran at a pH > PI of the target molecule).
The FP grade resin is based on a highly crosslinked polymethacrylic matrix, which is hydroxylated for added hydrophilicity. This matrix is
functionalized with a diethyl-amino to form weak basic anion exchanger. This resin is used in the large scale separation of anionic proteins,
peptides, and polynucleotides (separation ran at a pH > PI of the target molecule) as well as enzyme immobilization supports. This methacrylic
matrix has excellent mechanical strength with wide range pH stability (1 to 13) and resistance to high temperatures (up to 60° C operating
temperature). Unique all purpose ion-exchange resins. The diethylamine (DEA) functionality is a secondary amine base group which provides
increased capacity towards target molecules.
Product Mean
Size
Particle
Range
Funct.
Group
Counter
Ion
Functional
Degree
Exclusion
Limit
Capacity
BSA
Pore
Diamtr.
Column
Dim.
Packing
Sizes
CQA35S 10 μm 9-11 μm QA Cl- 0.3-0.5 meq/g Up to 106 - 600 Å 75 x 75 mm 10, 25, 50 g
CQA35P 30 μm 25-35 μm QA Cl- 0.3-0.5 meq/g Up to 106 - 600 Å - 25, 50, 100,
1000 ml
CQA31S 10 μm 9-11 μm DEAE Free Base 0.5-0.7 meq/g Up to 106 - 600 Å 75 x 75 mm 10, 25, 50 g
CQA31P 30 μm 25-35 μm DEAE Free Base 0.5-0.7 meq/g Up to 106 - 600 Å - 25, 50, 100,
1000 ml
FP-DA13 120 μm 100-150 μm Diethyl
amine
Free Base 1.9 meq/ml Up to 106 15-20 mg/ml 600 Å - 500 ml, 40 L
Product Matrix Mean Size Particle Range Pore Diameter Column Dimension Packing Sizes
CHP20P/P20 Styrene-DVB 20 μm 18-22 μm 450 Å - 25, 100, 1000 ml
CHP20P/P30 Styrene-DVB 30 μm 20-40 μm 450 Å - 25, 100, 1000 ml
CHP20/P50 dry Styrene-DVB 50 μm 37-75 μm 450 Å - 100 g
CHP07/P10 Bromintated
Styrene-DVB
10 μm 9-11 μm 250 Å 4.6 x 150 mm
10 x 250 mm
20 x 150 mm
20 x 250 mm
500 ml, 40 L
CMG20/P10 Methacrylate 10 μm 9-11 μm 250 Å 4.6 x 150 mm
10 x 250 mm
20 x 150 mm
20 x 250 mm
10 g, 1000 g
CMG20/P30 Methacrylate 30 μm 25-33 μm 250 Å - 25, 100, 1000 ml
Styrenic & Methacrylic Reversed Phase Resins for Preparative Chromatography
Styrenic Type: MCI GEL® CHP20P/P20, CHP20/P30, CHP20/P50 dry
Brominated Type: MCI GEL® CHP07/P10
Methacrylate Type: MCI GEL® CMG20/P10, CMG20/P30
The CHP grade resins are based on a highly crosslinked polystyrene matrix with no functionality. These resins offer varying pore size, surface area,
and bead size to allow the chromatographer to control the efficiency and the resolution in the separation of pharmaceuticals (e.g. heterocyclic
compounds), steroids, small peptides and proteins, oligonucleotides, amphoteric molecules (e.g. sulfonamides, antibiotics, nucleic bases), basic
drugs (e.g. anticonvulsants), simple amines (e.g. catecholamine), and antihistamines, etc. These styrenic matrices have excellent mechanical
strength with wide range pH stability (1 to 14) and resistance to high temperatures (up to 120° C operating temperature). CHP20 grades are the
preparative size for HP20. CHP07 grades are the preparative size for SP207.
The CMG grade resins are based on highly crosslinked polymethacrylic matrix with no functionality. Polymethacrylate resins have no aromatic
character, offering medium hydrophobicity (less hydrophobic than styrenic resins or octadecyl silane). They are mainly used in the separation of
pharmaceuticals (e.g. polyaromatic and polyaliphatic compounds), water soluble vitamins, small peptides and proteins, oligonucleotides, simple
amines (e.g. catecholamine), and agricultural chemicals, etc. These methacrylic matrices have excellent mechanical strength and wide range pH
stability (1 to 13), and resistance to high temperatures (up to 120° C operating temperature). CMG grades are the preparative size for HP2MG.
PolymericResins

29
Bioseparation: Wide Pore Hydrophilic Cation Exchange Resins
SP (Sulfopropyl) Type: MCI GEL® CQK30S, CQK30P
CM (Carboxymethyl)Type: MCI GEL® CQK31S, CQK31P
The CQ grade resins are based on highly crosslinked polymethacrylic matrix, which is further hydroxylated for added hydrophilicity.These matrices
are functionalized with either sulfonic acid or carboxymethyl to form strong acid and weak acid cation exchangers respectively with a sodium
counterion. By varying the bead size of these resins, the chromatographer can control the efficiency and the resolution in the separation of cationic
proteins, peptides, and polynucleotides (separation at a pH < PI of the target molecule). These methacrylic polymer matrices have excellent
mechanical strength with wide range pH stability (1 to 13) and resistance to high temperatures (can be autoclaved at 121° C for 20 minutes).
Product Mean
Size
Particle
Range
Funct.
Group
Counter
Ion
Functional
Degree
Exclusion
Limit
Pore
Diam.
Column
Dimension
Packing Sizes *
CQK30S 10 μm 9-11 μm SP Na+ 0.3-0.5 meq/g Up to 106 600 Å 75 x75 mm 10, 25, 50 g
CQK30P 30 μm 25-35 μm SP Na+ 0.3-0.5 meq/g Up to 106 600 Å - 100 ml
CQK31S 10 μm 9-11 μm CM Na+ 0.2-0.4 meq/g Up to 106 600 Å 75 x 75 mm 10, 25, 50 g
CQK31P 30 μm 25-35 μm CM Na+ 0.2-0.4 meq/g Up to 106 600 Å - 100 ml
Strong Acidic Cation Exchange Resins
Gel Type: Diaion® SK1B, UBK10
Porous Type: Diaion® PK228, RCP160M
Strong Acidic Cation Exchange Resins are cross-linked polystyrene resins having sulfonic acid groups. Among the Diaion® Product line, there are
SK grades of gel type, PK grades of porous type, RCP160M of highly porous type. The standard shipping is sodium salt form. In some cases, the
hydrogen form is available for catalysis applications such as esterification, alkylation, hydrolysis, hydration, etc.
SK1B in Na+ and H+ form resin is our “flagship” premium grade, gel-type, strong acid cation resin. It has excellent properties for industrial
applications and is recommended for industrial scale softening and de-mineralization applications. Featuring standard crosslinkage as is used in
situations where the raw water contains oxidizing substances.
The PK resin grades are based on a porous styrene DVB polymer matrix. Their porous structure provides excellent durability against osmotic
shock by swelling and shrinkage. PK228 is recommended for condensate de-mineralization (L grade is recommended for best pressure flow
performance). PK grades area also recommended for special applications such as de-ashing, de-colorization, and as a catalyst (as they have higher
reaction rate in organic solvents than gel type resins).
Unlike other ion-exchange resins, the UBK ion-exchange resin featuring good uniformity in particle size, reducing the reagent costs and spent
waste waters useful where high purity water is needed for manufacturing processes. UBK ion-exchange resins are also used in many other fields
because they have few large particles which can be easily broken by shrinkage and swelling.
Diaion® RCP160M is based on a macroporous crosslinked polystyrene matrix with sulfonic acid functional groups. This matrix is more open and
porous than conventional grade resin with increased polymer surface available for intimate contact with solutes. In catalysis, the reaction rates are
faster with RCP160M than standard grade resins, resulting in a higher degree of conversion during catalytic applications.
Product Matrix Counter Ion Total Capacity
(meq/ml-r)
Water
Retention (%)
Shipping
Density (g/L)
SK1B* Gel (styrene, DVB):
R-SO3-Na+
Na+ for standard form, H+ for catalyst
applications and de-mineralization
>2.0 Na form
>1.7 H form
43–50 Na form
50-60 H form
~ 825 Na form
~ 780 H form
PK228* Porous (styrene, DVB):
R-SO3- Na+
>2.05 Na form
>1.9 H form
37-43 Na form
39-49 H form
~ 805 Na form
~ 770 H form
UBK10 Gel (styrene, DVB):
R-SO3- Na+
> 2.2 Na form 35-45 Na form ~ 850 Na form
RCP160M Highly Porous styrene,
DVB R-SO3- H+
>1.5 H form 45-55 H form -
Product Particle Size
Distribution
Effective
Size (mm)
Uniformity
Coefficient
Whole Bead
Count
Operating Temp. Crosslinked
SK1B* On 1180 µm: 5% max
Thru 300 µm: 1% max
0.40 min 1.6 max 90% min 120° C (H form, Na form) max 8%
PK228* On 1180 µm: 5% max
0.40 min 1.6 max 95% min 120° C (H form, Na form) max 14%
UBK10 580 ± 20 µm - 1.2 max 90% min 120° C (H form, Na form) max 10%
RCP160M On 710 µm: 25% max
- 1.6 max 95% min 120° C (H form, Na form) max High
PolymericResins

30
Product Matrix Counter Ion Total Capacity
(Cl- form)
Water Retention
(Cl- form)
Shipping Density
(Cl- form)
SA10A Gel (styrene, DVB):
R-CH2- N(CH3)3+
Cl- for standard form, OH- for catalyst
> 1.3 meq/ml-R 43% - 47% ~ 670 g/L
PA308 Porous (styrene, DVB):
R-CH2- N(CH3)3+
Cl- for standard form, OH- for catalyst
> 1.0 meq/ml-R 57% - 67% ~ 710 g/L
HPA25 Highly Porous Na+ for standard form, H+ for catalyst
> 0.5 meq/ml-R 58% - 68% ~ 680 g/L
SA20A Gel (styrene, DVB) - >1.3 meq/ml-R 45% - 52% ~ 715 g/L
PA418 Porous (styrene, DVB) - >1.3 meq/ml-R 38% - 44% ~ 685 g/L
Product Matrix Total Capacity Water Retention Shipping Density Effective pH Range
WK100 Porous - Methacrylic >2.8 meq/ml-R 45% - 55% ~ 660 g/L 5 - 14
WK60L Acrylic 4.4 meq/ml-R (min.) 44% - 52% ~ 800 g/L 4-14
Strong Basic Anion Exchange Resins
Gel Type: Diaion® SA10 (Type I), SA20A (Type II)
Porous Type: Diaion® PA308 (Type I), PA418 (Type II), HPA25 L (Type I)
Diaion® Strong base anion exchange resins are based on a cross-linked polystyrene matrix having quaternary ammonium groups (NR3+). Among
the Diaion® product line, there are SA-grades of gel type, PA-grades of porous type, and HPA-grades of highly porous-type. The standard shipping
is Cl- form (in some cases OH- form is available). Type I resins have trimethyl ammonium groups which impart higher basicity. In the co-current
regeneration mode, the treated water will have the lowest silica leakage. Type I resins also have higher chemical stability and can be applied at
higher temperature than Type II or acrylic based resins. SA10A is a Type I resin with standard crosslinkage.
Porous-type ion exchange resins of a porous polymer matrix have good resistance against swelling and shrinking, though their exchange capacity
is lower than gel-type ion exchange resins of the same degree of crosslinkage. These are effective when highly purified effluents are needed,
e.g. removal of silica to very low concentration. They are suitable for treatments of waste waters with organic compounds. PA308 is used for de-
mineralization and de-colorization of sugar liquors.
Product Functionality Particle Size
Distribution
Effective
Size
Uniformity
Coefficient
Whole
Bead Count
OperatingTemp. Cross-
linked
SA10A - On 1180 µm: 5% max
0.40 mm
min
1.6 max 90% min 60° C (OH form)
80° C (Cl form) max
-
PA308 - On 1180 µm: 5% max
0.40 mm
min
4%
HPA25 - On 250 µm: 9v5% min 0.25 mm
min
High
SA20A R-CH2- N(CH3)2(CH3CH2OH)
+ Cl- for standard form
On 1180 µm: 5% max
0.40 mm
min
-
PA418 R-CH2- N(CH3)2(CH3CH2OH)
+ Cl- for standard form
On 1180 µm: 5% max
0.40 mm
min
9%
Product Functionality Particle Size Distribution Effective Size Uniformity
Coefficient
Whole Bead Count Operating
Temp.
WK100 Carboxylic
Acid
On 1180 µm: 5% max
0.40 mm min 1.6 max 95% min 150° C max
WK60L Carboxylic
Acid
On 1180 µm 10% (max)
Thru 425 µm 3%
0.40 mm min 1.6 max 95% min 120° C (max)
(Na and H form)
Weakly Acidic Cation Exchange Resins
Methacrylic Type: Diaion®WK100
Acrylic Type: Diaion® WK60L
WK100 (methacrylic type) is a weak acid cation exchange resins having carboxylic acid functionalities. It has a pK value of approximately 6. Used
mainly used for the purification of pharmaceuticals and foods.
WK60L (acrylic type) is a weak acid cation exchange resin with carboxylic acid functionality based on porous acrylic polymer matrix. They have
a pK value of approximately 5.3. The acrylic type resins have higher total capacity than methacrylic type resins. They are mainly used for water
treatment applications such as the removal of hardness ions in the presence of bicarbonate alkalinity.
PolymericResins

Chromatography Products Catalog 2014

Chromatography Products Catalog 2014

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