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Dr. Mussarat
BLOOD TEST
Blood test
• Laboratory analysis performed on a blood sample
that is taken from vein
• To check glucose level, hemoglobin level, white blood
cells etc.
Methods of blood Tests
• Mainly two methods
• Extraction through
injection for large amount
of blood
• Finger prick for small
amount of blood
Different types of blood test
1. Troponin
2. Blood glucose
3. Full blood count
4. Cholesterol
5. Liver blood test
6. Group & save test
7. Urea & electrolyte test
8. Brain Natriuretic Peptide
9. Blood gas test
10. International Normalised Ratio
11. Blood group determination test
Troponin
• If you have chest pain, a blood
test will often be taken to check
your level of troponin,
• troponin, a protein which the
heart releases into your
bloodstream when the heart
muscle is being damaged.
• It can help to diagnose a heart
attack, but a low level doesn’t
prove you haven’t had a heart
attack.
Troponin
• Women tend to produce less troponin than
men, so a heart attack diagnosis may be
missed. The BHF has funded research
looking at ways of measuring the same
protein but at much lower levels, known as
high-sensitivity testing. Troponin is always
tested in a hospital setting. The results are
usually available quickly which allows
prompt diagnosis and treatment.
Troponin
• Troponin test is also known as T-test
• Troponin T test
• Troponin L test
• Normal range: below 0.04 ng/ml
• Probable heart attack: above 0.40 ng/ml
• Having a result between 0.04 and 0.39 ng/ml
often indicates a problem with the heart.
Blood glucose
Also known as HbA1c
This checks your blood glucose level.
 It helps give an accurate diagnosis of diabetes and guide your
doctor to the best treatment for you.
 If the levels are slightly higher than normal but
don’t meet the criteria for diabetes, your doctor
might say you have pre-diabetes or ‘borderline’
diabetes. This means you are still at risk
Blood glucose
Normal fasting 70-100mg/dl
 random 125mg/dl
Pre-diabetic fasting 100-125 mg/dl
 random 140-199 mg/dl
 Diabetic fasting 126mg/dl
 random 200mg/dl
Cholesterol Test
• Know as lipid profile test
• Amount of cholesterol or
fat or lipid protein can be
calculated
• Two types
• Good cholesterol
• Bad cholesterol
Good cholesterol
• Known as low density lipoprotein
• Also known as LDL
• Main source of blockage arteries
• Less than 100mg/dl optimal
• 100-129mg/dl near optimal
• 130-159mg/dl borderline
• 160-189mg/dl high
• 190mg/dl or above very high
Bad cholesterol
• Known as high density lipoprotein
• Also known as HDL
• Helps to get rid of bad cholesterol
• 60mg/dl and high considered protective
against heart disease
• 40-59mg/dl high, better
• Less than 40mg/dl major risk of heart attack
• It is also a good way to see if your
medication is being effective in reducing
your bad cholesterol levels, and in
particular to see if your dose needs to be
increased. Many people only need this
checking once a year, but your doctor
may want to keep a closer eye on it if it
remains high despite treatment, or refer
you to a doctor who specialises in lipid
management.
Liver blood tests
• To diagnose liver disease
• Also known as liver function test
• LBT
• LFT
• Done to make sure that liver is working properly
Liver blood tests
• The test looks at several different
enzymes and proteins, which are
known to be higher or lower if
the liver is damaged. An
abnormal test result doesn’t
necessarily mean that your liver
isn’t working properly.
Full blood count
• To check different
components of blood like
RBC, WBC, hemoglobin
etc.
• Also known as total blood
count
• FBC
• TBC
Full blood count
• RBC 4.32-5.72 million cells/ml men
• RBC 3.90-5.03 million cells/ml women
• Platelets 150,000-450,000/mcl
• WBC 3500-10,500/mcl
• Hemoglobin 13.5-17.5g/ml men
• Hemoglobin 12.0-15.5.5g/ml women
Full blood count
• Detect iron deficiency
• Infection
• Heart condition
• Cancer
• Anemia
Group and save test
• Also known as group and screen test
• This is done if you might need a blood transfusion later, for example
if you are having surgery or have severe anemia.
• It finds out your blood group and checks for antibodies that can
affect a transfusion. It is done in the hospital at your pre-
assessment clinic, or if you are admitted as an emergency, after an
accident for example. It needs be done less than 72 hours before
the potential transfusion, so will be repeated if necessary.
Urea and electrolytes
• These are important biological tests
• Electrolytes Maintain the pH of blood
• Sodium 135-145mmol/L
• Potassium 5.1-6.0mmol/L
• Magnesium
• Ammonia 15-50mmol/L
• Urea 15-40mg/dl
• Blood urea nitrogen 2.5-6.4mmol/L
Urea and electrolytes
• If the levels are too high or too low, this can cause
abnormal heart rhythms, so it is important to adjust
them if the levels aren’t right.
• The body makes a protein called urea which is normally
broken down in the kidneys. If there is a higher level in
the blood than normal, this could indicate problems
with kidney function, which can affect your heart too.
• Some heart medications, such as ACE
inhibitors, can affect your kidneys, so this
test will help your doctor decide if it is
safe or appropriate to increase your dose,
depending on your condition and the
results of the test.
Brain Natriuretic Peptide
• This can diagnose or show the
severity of heart failure.
• If you are showing symptoms of heart
failure, such as swollen ankles or
feeling short of breath, having this
blood test
• Peptide is a hormone that is released
in response to volume expansion and
increase wall stress of the cardiac
myocytes.
BNP Continued……
• BNP level is high when heart can not
pump the way it should.
• Above 100pg/ml is abnormal and
dangerous
Blood gas test
• Blood is sometimes taken from an artery
in the wrist to check for levels of oxygen
and carbon dioxide, and its chemical
balance. This would only be done in places
like A&E and intensive care units. The test
is used if you have trouble breathing or
have lung problems.
• Also known as arterial blood gas
• ABG
Blood gas test
• Determine the pH of blood
• pH 7.35-7.45
• Pressure of carbon dioxide 33-45 mmHg
• Pressure of oxygen 80-100 mmHg
• Oxygen saturation 95%
• Bicarbonates 22-26mEq/L
• Check lung disease
International Normalised Ratio
• If you take warfarin (a blood-thinning
medicine), it is very important that your
blood clotting levels are kept within a level
requested by your specialist.
• Measure how long to clot blood after injury
• Normal 1.1 or below
• 2.0-3.0 effective if taking blood thinning
medicine like warfarin
• 4.9 critical
Why Should I Know About Blood Conditions?
• When something is wrong with your blood, it can affect
your total health.
• That is why it is important for you to know about some
of the common blood disorders that may affect you.
• People may be affected by many different types of
blood conditions and blood cancers.
• Common blood disorders include anemia, bleeding
disorders such as hemophilia, blood clots, and blood
cancers such as leukemia and lymphoma.
Other Blood Tests
• Calcium blood test
• Calcium is important because it gives strength to
your bones. Your muscles, nerves and heart need
calcium to work properly, while calcium also helps
your blood to clot.
• 8.6-10.2mg/dl
• Vary lab to lab
Vitamin D test
• Vitamin D is important for strong and
healthy bones, and can help prevent a
number of diseases. Vitamin D levels in
your body can be checked with a blood
test.
• 20-50ng/mL
• Less than 12ng/mL indicate deficiency
Vitamin B12 test
• You need vitamin B12 in your blood so you can make
blood cells and have your nerves function well.
Vitamin B12 is also known as cobalamin.
• 200-900pg/mL
• 200-300pg/mL is boarderline
Iron studies
• Iron studies are blood tests that look at
how much iron is in your blood and in
other cells. Having either too little or too
much iron can cause serious problems.
• 60-170mcg/dL
• Low conc. cause anemia
• High conc. Cause iron overload disease
Kidney function tests
• Your kidneys are important because they filter waste
from your body and regulate the salts in your blood.
There are many reasons your kidneys may not work
effectively. Kidney function tests can help your doctor
check your kidney function and to monitor it over time.
• Creatinine range 0.6–1.1 mg/dL in women and 0.7–1.3
mg/dL in men.
• Urea, ureic acid, phosphate can also b checked
REFERENCES
1. https://www.healthline.com/health/blood-tests#important-blood-tests
2. Laboratory Manual and Review on Clinical Pathology, Dr. Jelalu Kemal,
3. https://www.healthdirect.gov.au/blood-tests-a-z

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Blood tests

  • 2. Blood test • Laboratory analysis performed on a blood sample that is taken from vein • To check glucose level, hemoglobin level, white blood cells etc.
  • 3. Methods of blood Tests • Mainly two methods • Extraction through injection for large amount of blood • Finger prick for small amount of blood
  • 4. Different types of blood test 1. Troponin 2. Blood glucose 3. Full blood count 4. Cholesterol 5. Liver blood test 6. Group & save test 7. Urea & electrolyte test 8. Brain Natriuretic Peptide 9. Blood gas test 10. International Normalised Ratio 11. Blood group determination test
  • 5. Troponin • If you have chest pain, a blood test will often be taken to check your level of troponin, • troponin, a protein which the heart releases into your bloodstream when the heart muscle is being damaged. • It can help to diagnose a heart attack, but a low level doesn’t prove you haven’t had a heart attack.
  • 6. Troponin • Women tend to produce less troponin than men, so a heart attack diagnosis may be missed. The BHF has funded research looking at ways of measuring the same protein but at much lower levels, known as high-sensitivity testing. Troponin is always tested in a hospital setting. The results are usually available quickly which allows prompt diagnosis and treatment.
  • 7. Troponin • Troponin test is also known as T-test • Troponin T test • Troponin L test • Normal range: below 0.04 ng/ml • Probable heart attack: above 0.40 ng/ml • Having a result between 0.04 and 0.39 ng/ml often indicates a problem with the heart.
  • 8. Blood glucose Also known as HbA1c This checks your blood glucose level.  It helps give an accurate diagnosis of diabetes and guide your doctor to the best treatment for you.  If the levels are slightly higher than normal but don’t meet the criteria for diabetes, your doctor might say you have pre-diabetes or ‘borderline’ diabetes. This means you are still at risk
  • 9. Blood glucose Normal fasting 70-100mg/dl  random 125mg/dl Pre-diabetic fasting 100-125 mg/dl  random 140-199 mg/dl  Diabetic fasting 126mg/dl  random 200mg/dl
  • 10. Cholesterol Test • Know as lipid profile test • Amount of cholesterol or fat or lipid protein can be calculated • Two types • Good cholesterol • Bad cholesterol
  • 11. Good cholesterol • Known as low density lipoprotein • Also known as LDL • Main source of blockage arteries • Less than 100mg/dl optimal • 100-129mg/dl near optimal • 130-159mg/dl borderline • 160-189mg/dl high • 190mg/dl or above very high
  • 12. Bad cholesterol • Known as high density lipoprotein • Also known as HDL • Helps to get rid of bad cholesterol • 60mg/dl and high considered protective against heart disease • 40-59mg/dl high, better • Less than 40mg/dl major risk of heart attack
  • 13. • It is also a good way to see if your medication is being effective in reducing your bad cholesterol levels, and in particular to see if your dose needs to be increased. Many people only need this checking once a year, but your doctor may want to keep a closer eye on it if it remains high despite treatment, or refer you to a doctor who specialises in lipid management.
  • 14. Liver blood tests • To diagnose liver disease • Also known as liver function test • LBT • LFT • Done to make sure that liver is working properly
  • 15. Liver blood tests • The test looks at several different enzymes and proteins, which are known to be higher or lower if the liver is damaged. An abnormal test result doesn’t necessarily mean that your liver isn’t working properly.
  • 16. Full blood count • To check different components of blood like RBC, WBC, hemoglobin etc. • Also known as total blood count • FBC • TBC
  • 17. Full blood count • RBC 4.32-5.72 million cells/ml men • RBC 3.90-5.03 million cells/ml women • Platelets 150,000-450,000/mcl • WBC 3500-10,500/mcl • Hemoglobin 13.5-17.5g/ml men • Hemoglobin 12.0-15.5.5g/ml women
  • 18. Full blood count • Detect iron deficiency • Infection • Heart condition • Cancer • Anemia
  • 19. Group and save test • Also known as group and screen test • This is done if you might need a blood transfusion later, for example if you are having surgery or have severe anemia. • It finds out your blood group and checks for antibodies that can affect a transfusion. It is done in the hospital at your pre- assessment clinic, or if you are admitted as an emergency, after an accident for example. It needs be done less than 72 hours before the potential transfusion, so will be repeated if necessary.
  • 20. Urea and electrolytes • These are important biological tests • Electrolytes Maintain the pH of blood • Sodium 135-145mmol/L • Potassium 5.1-6.0mmol/L • Magnesium • Ammonia 15-50mmol/L • Urea 15-40mg/dl • Blood urea nitrogen 2.5-6.4mmol/L
  • 21. Urea and electrolytes • If the levels are too high or too low, this can cause abnormal heart rhythms, so it is important to adjust them if the levels aren’t right. • The body makes a protein called urea which is normally broken down in the kidneys. If there is a higher level in the blood than normal, this could indicate problems with kidney function, which can affect your heart too.
  • 22. • Some heart medications, such as ACE inhibitors, can affect your kidneys, so this test will help your doctor decide if it is safe or appropriate to increase your dose, depending on your condition and the results of the test.
  • 23. Brain Natriuretic Peptide • This can diagnose or show the severity of heart failure. • If you are showing symptoms of heart failure, such as swollen ankles or feeling short of breath, having this blood test • Peptide is a hormone that is released in response to volume expansion and increase wall stress of the cardiac myocytes.
  • 24. BNP Continued…… • BNP level is high when heart can not pump the way it should. • Above 100pg/ml is abnormal and dangerous
  • 25. Blood gas test • Blood is sometimes taken from an artery in the wrist to check for levels of oxygen and carbon dioxide, and its chemical balance. This would only be done in places like A&E and intensive care units. The test is used if you have trouble breathing or have lung problems. • Also known as arterial blood gas • ABG
  • 26. Blood gas test • Determine the pH of blood • pH 7.35-7.45 • Pressure of carbon dioxide 33-45 mmHg • Pressure of oxygen 80-100 mmHg • Oxygen saturation 95% • Bicarbonates 22-26mEq/L • Check lung disease
  • 27. International Normalised Ratio • If you take warfarin (a blood-thinning medicine), it is very important that your blood clotting levels are kept within a level requested by your specialist. • Measure how long to clot blood after injury • Normal 1.1 or below • 2.0-3.0 effective if taking blood thinning medicine like warfarin • 4.9 critical
  • 28. Why Should I Know About Blood Conditions? • When something is wrong with your blood, it can affect your total health. • That is why it is important for you to know about some of the common blood disorders that may affect you. • People may be affected by many different types of blood conditions and blood cancers. • Common blood disorders include anemia, bleeding disorders such as hemophilia, blood clots, and blood cancers such as leukemia and lymphoma.
  • 29. Other Blood Tests • Calcium blood test • Calcium is important because it gives strength to your bones. Your muscles, nerves and heart need calcium to work properly, while calcium also helps your blood to clot. • 8.6-10.2mg/dl • Vary lab to lab
  • 30. Vitamin D test • Vitamin D is important for strong and healthy bones, and can help prevent a number of diseases. Vitamin D levels in your body can be checked with a blood test. • 20-50ng/mL • Less than 12ng/mL indicate deficiency
  • 31. Vitamin B12 test • You need vitamin B12 in your blood so you can make blood cells and have your nerves function well. Vitamin B12 is also known as cobalamin. • 200-900pg/mL • 200-300pg/mL is boarderline
  • 32. Iron studies • Iron studies are blood tests that look at how much iron is in your blood and in other cells. Having either too little or too much iron can cause serious problems. • 60-170mcg/dL • Low conc. cause anemia • High conc. Cause iron overload disease
  • 33. Kidney function tests • Your kidneys are important because they filter waste from your body and regulate the salts in your blood. There are many reasons your kidneys may not work effectively. Kidney function tests can help your doctor check your kidney function and to monitor it over time. • Creatinine range 0.6–1.1 mg/dL in women and 0.7–1.3 mg/dL in men. • Urea, ureic acid, phosphate can also b checked
  • 34. REFERENCES 1. https://www.healthline.com/health/blood-tests#important-blood-tests 2. Laboratory Manual and Review on Clinical Pathology, Dr. Jelalu Kemal, 3. https://www.healthdirect.gov.au/blood-tests-a-z