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Molisch’s test
Molisch’s test is a general test for the identification of all carbohydrates (monosaccharide, disaccharide,
and polysaccharide) and glycoprotein. In this test conc. sulphuric acid added to the solution which
hydrolyzes the all glycosidic linkage in the sugar molecules (disaccharide, and polysaccharide) to yield
monosaccharide, which in the presence of an acid get dehydrated to form furfural and its derivatives.
This is very reactive, and condenses with α-naphthol to give a purple or violet colored product.
Reagents:
 Molisch reagent (1 gm of α-nephthol in 10 ml of 95% ethyl alcohol and made final volume
up to 100 ml with distilled water)
 Concentrated H2SO4
Procedure:
 Take 3 ml of given sample in test tube.
 Add 2 -3 drops molisch reagent.
 Mix it and add 3 ml of conc. H2SO4 carefully along the wall of test.
 Gently rotate the test tube.
Observation: A purple-violet ring forms. Observation: no color change
Inference: carbohydrates present Inference: carbohydrates not present
Principle:
Carbohydrates are hydrolyzed by Concentrated H2SO4 into their derivatives which will condense with
α-nephthol to give purple color ring.

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Molischs test

  • 1. Molisch’s test Molisch’s test is a general test for the identification of all carbohydrates (monosaccharide, disaccharide, and polysaccharide) and glycoprotein. In this test conc. sulphuric acid added to the solution which hydrolyzes the all glycosidic linkage in the sugar molecules (disaccharide, and polysaccharide) to yield monosaccharide, which in the presence of an acid get dehydrated to form furfural and its derivatives. This is very reactive, and condenses with α-naphthol to give a purple or violet colored product. Reagents:  Molisch reagent (1 gm of α-nephthol in 10 ml of 95% ethyl alcohol and made final volume up to 100 ml with distilled water)  Concentrated H2SO4 Procedure:  Take 3 ml of given sample in test tube.  Add 2 -3 drops molisch reagent.  Mix it and add 3 ml of conc. H2SO4 carefully along the wall of test.  Gently rotate the test tube. Observation: A purple-violet ring forms. Observation: no color change Inference: carbohydrates present Inference: carbohydrates not present Principle: Carbohydrates are hydrolyzed by Concentrated H2SO4 into their derivatives which will condense with α-nephthol to give purple color ring.