This document discusses the use of nanoparticles in poultry nutrition. It begins with an introduction to nanotechnology and how it has been applied in various agricultural sectors like meat, eggs, fish, and dairy. It then discusses different types of nanoparticles like inorganic, organic, metal oxide, and carbon-based nanoparticles. The document outlines various ways nanoparticles can benefit poultry through improving feed efficiency, controlling nutrient absorption, and promoting growth. It also discusses challenges in the use of nanoparticles and concludes that nanoparticles show promising results in improving poultry performance, health, egg quality, and gut health.

1. YYU
Faculty of Agriculture
Animal Science Department
Using of Nanoparticles in
Poultry Nutrition
Prepared by :
Jotyar Hasan Younis
MSc ın poultry Nutrition

2. INTRODUCTION
Nanotechnology
Is the promising and emerging technology
that has many potential to
breakthrough livestock and agro-food sector.
(Siddiqi et al , 2016 ) AGRI-FOOD
• MEAT AND EGG
• FRUITS
• FISH
• OIL AND FACTS
• DAIRY PRODUCT

3. INTRODUCTION
Nanotechnology
(He et al., 2019)
Nanotechnology
Food
Processing
Food
Preservation
Crop
Productivity
Feeding
Animal
Environmental
Monitoring
It has been employed in animal
feeding, food processing and
preservation, crop productivity,
and environmental monitoring .

4. INTRODUCTION
Nanoparticles
 Refer to a particle size of 1–100 nm, in which the physical,
chemical and biological properties of materials in fact are differ
from their bulk form )Sampathkumar et al ,. 2020).
Used in animal production as a nutritional supplements , drugs
and vaccines (Jampilek et al ,.2019).

5. THE AIM OF USING OF ANOPARTICLES
IN POULTRY SECTOR
 Improving feed efficacy,
 Controlling nutrient absorption,
 Counteracting pathogens,
 Growth promoting,
 Modifying the contents of the egg like full
protein or cholesterol free eggs,
(Gallocchio et al ,. 2015).
NANO FEED
SILVER
SELENIUM
ZINK

6. CLASSIFICATIN OF NANOPARTICLES
ACCORDING TO ITS NATURE
Nanoparticles
Inorganic
Metal Oxide
Metal
Carbone
Based
 Nano diamonds
 Fullerens
 Carbone
 Nanotube
 Graphene
 Carbone nanofiber
 Zinc oxide
 Iron oxide
 Zinc
 Iron
 Copper
 Cobalt
 Silver
 Gold
...etc.
 Liposome
 Protiens
 Peptide based
Organic
(Marappan et al ,. 2017 )

7. Bottom up
• Super critical fluid synthesises
• Spinning
• Sol-gel process
• Laser pyrolysis
• Chemical vapour deposition
• Chemical reducing
Top down
• Mechanical milling
• Etching
• Laser ablation
• Sputtering
• Electro explotion
Nuclei and its growing
Molecular – Atomic level
Nano particles
Bulk material
APPROACHES OF NANOMATERIALS SYNTHESIS
( Baig et al ,. 2021)

8. MECHANISM OF NANOPARTICLES ACTION
The Action of Nano Particles :
 Increase the surface area of gut for better interaction ,
 Extending the residence time in gut of materials ,
(Chen et al.2006).
 Reducing the intestinal clearance mechanisms ,
 Increasing tissues penetration,
 Efficient cells uptake and
effective delivery to target sites

9. APPLICATION OF NANOPARTICLE IN POULTRY NUTRITION
Nano
particles
Animal
Species
Effect References
Silver
5 g/kg
Feed supliment
Broiler
chicken
 Increased phago cytosis and leukocyte
metabolic activity
 Antioxidant activity was enhanced with
decreasing level of haemoglobin
 Increased lipid peroxidation
(Ognik et
al,.2016)
Silver
5 mg/L
Drinking Water
Broiler
chicken
 Decreased villus height to crypt depth
ratio in the jejunum
 Stimulated and activated immune with
enhanced oxidative stress system
(Kulak et al
,. 2018 )
Silver
50 mg/L
Drinking Water
Broiler
chicken
 Reducing the rate of mortality
 Increase in growth of chicken
(Kumar et al
,. 2020)

10. Nano
particles
Animal
Species
Effect References
Zn
20, 40, 60, 80 and
100
mg /kg
Feed supplement
Layer
chicken
 Eggshell thickness and Eggshell Ca and
P contents were significantly improved .
 Excreta Zn content decreased linearly
when hens were fed nano Zn .
(Cufadar et
al ,. 2020 )
Zn
45, 90, 135 and
180 mg/kg
Feed supliment
Broiler
chicken
 Positive effect on performance and
Antioxidant activity
(Hidayat et
al. (2021)
Zn
30, 50, 70 and 90
mg/kg
Feed supplement
Broiler
chicken
 Improvement of performance and
immunity parameters
(Eskandani
et al. 2021)
APPLICATION OF NANOPARTICLE IN POULTRY NUTRITION

11. Nano
particles
Animal
Species
Effect References
CU
50 g /kg
Feed supplement
Broiler
chicken
 Improved meat quality by increased
antioxidant levels and reduction of lipid
peroxidation.
 There were significantly lower
Total Saturated Fatty Acid Levels
(Galli et al. ,
2020)
CuNPs
10 mg/L
Drinking Water
Broiler
chicken
 Improved immunity and growth
performance
(El-Kazaz
and Hafez
2020)
CuNPs
0.3 mg/L
In ovo injection
Broiler
chicken
 Positive effect on performance
 Decrease of FCR and mortality
(Mroczek-
Sosnowska
et al. 2015)
APPLICATION OF NANOPARTICLE IN POULTRY NUTRITION

12. Nano-Se
0.3, 0.9 and 1.5
mg/kg
Feed supplement
Broiler
chicken
 Improve performance
 Improved gut health by increasing the
population of beneficial bacteria.
(Gangadoo
et al ,. 2018)
Nano-Se
0.1, 0.2, 0.3, 0.4
and 0.5 mg/kg
Feed supliment
Broiler
chicken
 Improved growth performance and
immunity parameters .
(Ahmadi et
al. (2018
Nano-Se
0.4 g/kg
Feed supliment
Broiler
chicken
 Decreased total bacterial count, total
yeast and molds count
 Increased lactic acid bacteria
(Alagawany
et al. 2021)
Nano
particles
Animal
Species
Effect References
APPLICATION OF NANOPARTICLE IN POULTRY NUTRITION

13. Nano
particles
Animal
Species
Effect References
Iron
4 Mg/Kg
Feed supplement
Broiler
Chicken
 Significant iincrease of Body Weight )P ≤
0.05).
 Increase In the Level of Erythrocytes (P
≤ 0.01),
(Abdullah
et.al. 2022 )
Nano-Gold
5 and 15 mg/L
Drinking water
Broiler
Chicken
 Lower NPs dose had a positive effect on
growth performance (average body
weight and the feed conversion ratio)
(Hassanen et
al. 2020)
Platinum NPs
1, 5, 10, 15 and 20
mg/ml –
In ovo injection
Broiler
Chicken
 Weight of liver decreased (p < 0.05) (Prasek et al.,
2013 )
APPLICATION OF NANOPARTICLE IN POULTRY NUTRITION

14. APPLICATION OF NANOPARTICLE IN POULTRY NUTRITION
Nano
particles
Animal
Species
Effect References
Ca carbonate
nanoparticles
1g/L
Drinking water
Layer
Chicken
 Produced strong eggshell. (Wang et al.
2017).
Ca carbonate
nanoparticles
100 and 200 ppm
CCN /egg
in ovo injection
Broiler
Chicken
 Body weight and spleen weight of
hatched chickens improved (P<0.05).
 Increased plasma alkaline phosphatase
(ALP) activity and bone Cu
concentration of hatched chickens
(P<0.05).
(Salary et al
,. 2017 )

15. CHALLENGES FACING USING NANOPARTICLES IN
PULTRY NUTRITION
 Potential toxicity of nanomaterials is not fully studied and
risk assessments including hazard identification, hazard
characterization and exposure assessment are essential
(Oberdörster et al., 2005)
 The meat of animals fed on these particles showed poor
quality due to low resistance to micro-organisms growth, poor
water retention and poor carcass traits (Fang et al., 2015).
 There is a lack of knowledge on the fate of ingested
nanoparticles in human body and it is essential to investigate
routes of exposure and also it is important to accumulate basic
knowledge on their absorption, distribution, metabolism,
and excretion (Bai et al., 2018).

16. COUNCLISION
 Nanoparticles it’s a new revolutionize livestock and agri-food,
 Its deal with particles that the dimension of it be between 0.1 to
100 nanometers,
 It has been used animal feed as a nutritional supplements
 Encouraging results aprove that using nanoparticles in poultry
promising on the performance, health,Improve Eggs quality in
layer,Improving gut health by increasing the population of
beneficial bacteria.

17. REFERENCES
• Siddiqi and A. J. N. Husen, “Fabrication of metal nanoparticles from fungi and metal salts: scope and application,”
Nanoscale Research Letters, vol. 11, no. 1, pp. 1–15, 2016
• He, X., Deng, H., & Hwang, H. M. (2019). The current application of nanotechnology in food and agriculture. Journal
of food and Drug Analysis, 27(1), 1-21.
• Sampathkumar, K., Tan, K. X., & Loo, S. C. J. (2020). Developing nano-delivery systems for agriculture and food
applications with nature-derived polymers. Iscience, 23(5), 101055.
• Jampilek J, Kos J and Kralova K (2019) Potential of nanomaterial applications in dietary supplements and foods for
special medical purposes. Nanomaterials, 9(2): 296.
• Gallocchio F, Belluco S, Ricci A (2015) Nanotechnology and Food: Brief overview of the current Scenario. Proc Food
Sci 5: 85-88ç
• Marappan Gopi, Beulah Pearlin, Ramasamy Dhinesh Kumar, Muthuvel Shanmathy and Govindasamy Prabakar, 2017.
Role of Nanoparticles in Animal and Poultry Nutrition: Modes of Action and Applications in Formulating Feed
Additives and Food Processing. International Journal of Pharmacology, 13: 724-731.
• Baig, Nadeem & Kammakakam, Irshad & Falath, W.S.. (2021). Nanomaterials: A review of synthesis, properties,
recent progress, and challenges. Materials Advances. 2. 10.1039/D0MA00807A.
• Chen H, Weiss J, Shahidi F (2006) Nanotechnology in nutraceuticals and functional foods. Food Technol 3: 30-36.
• K. Ognik, E. Cholewińska, A. Czech et al., “Effect of silver nanoparticles on the immune, redox, and lipid status of
chicken blood,” Czech Journal of Animal Science, vol. 61, no. 10, pp. 450–461, 2016.
• E. Kulak, K. Ognik, A. Stępniowska, and I. Sembratowicz, “The effect of administration of silver nanoparticles on
silver accumulation in tissues and the immune and antioxidant status of chickens,” Journal of Animal and Feed
Sciences, vol. 27, no. 1, pp. 44–54, 2018.
• Kumar, I.; Bhattacharya, J.; Das, B.K.; Lahiri, P. Growth, serum biochemical, and histopathological responses of
broilers administered with silver nanoparticles as a drinking water disinfectant. 3 Biotech 2020, 10, 1–12.

18. REFERENCES
• Cufadar, Y., Göçmen, R., Kanbur, G. et al. Effects of Dietary Different Levels of Nano, Organic and Inorganic Zinc
Sources on Performance, Eggshell Quality, Bone Mechanical Parameters and Mineral Contents of the Tibia, Liver,
Serum and Excreta in Laying Hens. Biol Trace Elem Res 193, 241–251 (2020).
• Eskandani M, Janmohammadi H, Mirghelenj SA, Ebrahimi M, Kalanaky S. 2021. Effects of zinc nanoparticles on
growth performance, carcass characteristics, immunity, and meat quality of broiler chickens. Iran J Appl Anim Sci.
11(1):135–146.
• Hidayat C, Sumiati S, Jayanegara A, Wina E. 2021. Supplementation of dietary nano Zn-phytogenic on performance,
antioxidant activity, and population of intestinal pathogenic bacteria in broiler chicken. Trop Anim Sci J. 44(1):90–99
• Galli, G.M.; Gerbet, R.R.; Griss, L.G.; Fortuoso, B.F.; Petrolli, T.G.; Boiago, M.M.; Souza, C.F.; Matheus, D.; Baldissera,
M.D.; Mesadri, J.; et al. Combination of herbal components (curcumin, carvacrol, thymol, cinnamaldehyde) in broiler
chicken feed: Impacts on response parameters, performance, fatty acid profiles, meat quality and control of coccidia
and bacteria. Microb. Pathol. 2020, 139, 103916.
• Kazaz S, Hafez M. 2020. Evaluation of copper nanoparticles and copper sulfate effect on immune status, behavior,
and productive performance of broilers. J Adv Vet Anim Res. 7(1):16–25.
• Mroczek-Sosnowska N, Łukasiewicz M, Wnuk A, Sawosz E, Niemiec J, Skot A, Jaworski S, Chwalibog A. 2015. In ovo
administration of copper nanoparticles and copper sulfate positively influences chicken performance: effect of Cu
on chicken performance. J Sci Food Agric. 96(9): 3058–3062
• gangadoo S, Dinev I, Chapman J, Hughes RJ, Van TTH, Moore RJ, Stanley D. 2018. Selenium nanoparticles in poultry
feed modify gut microbiota and increase abundance of Faecalibacterium prausnitzii. Appl Microbiol Biotechnol.
102(3):1455–1466
• Ahmadi M, Ahmadian A, Seidavi AR. 2018. Effect of different levels of nano-selenium on performance, blood
parameters, immunity and carcass characteristics of broiler chickens. Poult Sci J. 6(1):99–108ç

19. • Alagawany M, Abd El-Hack ME. 2021. Natural feed additives used in the poultry industry. Singapore: Bentham
Science Publishers Pte. Ltd.
• Abdullah SS, Masood S, Zaneb H, Rabbani I, Akbar J, Kuthu ZH, Masood A, Vargas-Bello-Pérez E. Effects of copper
nanoparticles on performance, muscle and bone characteristics and serum metabolites in broilers. Braz J Biol. 2022
Jun 17;84:e261578. doi: 10.1590/1519-6984.261578. PMID: 35730812.
• hassanen EI, Morsy EA, Hussien AM, Ibrahim MA, Farroh KY. 2020. The effect of different concentrations of gold
nanoparticles on growth performance, toxicopathological and immunological parameters of broiler chickens. Biosci
Rep. 40:BSR20194296
• prasek, M., Sawosz, E., Jaworski, S., Grodzik, M., Ostaszewska, T., Kamaszewski, M., et al. (2013). Influence of
nanoparticles of platinum on chicken embryo development and brain morphology. Nanoscale Research Letters,
8(1), 251.
• wang MJ, Chen Huang JT Huang Jw, Chen SE. 2017. Evaluation of the toxicity of nano-calcium carbonates and their
effects on antiheat stress in laying hens. J Chin Soc Anim Sci. 46:223–234
• Salary, J., Hemati Matin, H.R., Ghafari, K. and Hajati, H., 2017. Effect of in ovo injection of calcium carbonate
nanoparticles on bone post hatched characteristics and broiler chicken performance. Iranian Journal of Applied
Animal Science, 7(4), pp.663-667.
• Oberdörster G, Oberdörster E, Oberdörster, J (2005) Nanotoxicology: An emerging discipline evolving from studies
of ultrafine particles. Environ Health Perspect. 113: 823-839.
• Bai DP, Lin XY, Huang YF, Zhang XF (2018) Theranostics aspects of various nanoparticles in Veterinary Medicine. Int J
Mol Sci 19:1-32.
• Fang Z, Zhao, Y, Zhang M (2015) Current Practice and Innovations in Meat Packaging. Australian Meat Processing
Corporation, 1-97.
REFERENCES

20. Jotyar Hasan Younis
DOB: 18th NOV 1992
Correspondence Address:
İpekyolu/Van, Turkey
Phone number :(+964) 750-450-8478
(+90) 546-859-1208
E-mail: jotyarbady@gmail.com
Education:
• Bachelor at Animal production DEP from Faculty
of Agriculture in Duhok university
• Master student at zootecnical DEP in faculty of
Agriculture in Van Yuzuncu yil University