DV Biologics is a global supplier of human biological materials including a library of disease-specific and normal cells, media, and genomics products. They offer custom cell procurement and characterization services, and their quality-controlled products are used in drug discovery and development by biopharmaceutical companies and research institutes. DV Biologics provides a wide range of cellular systems, normal and diseased primary cells, proteomic and genomic tools, and optimized cell culture media to support research and drug development.

2. OVERVIEW
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3. ABOUT US
DV Biologics is a trusted global source
of human biological tools for drug
discovery and development
 Library of disease-specific and normal cells,
media and genomics products
 Custom cell procurement and characterization
services
Subsidiary of DaVinci Biosciences
 Discovers & developments cell-based
therapeutics for degenerative disease
 Holds multiple patents in the field of
stem cells and cell culture
 Well published scientific staff
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4. QUALITY & SELECTION
ISO 9001:2008 certified Quality Management System
Follow Good Laboratory Practices (GLP)
100% replacement guarantee when SOPs are
Largest selection of cells, RNA, cDNA, and Lysate in
industry
Largest selection of disease-specific products
Decades of expertise in cell culture and molecular
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5. OUR QUALITY & GLOBAL REACH
Product are available worldwide
13 global distributors including Cambridge Biosciences
(UK), Fisher Scientific (USA), and Cosmo Bio(JPN)
Strive to build long term relationships with our customers
Products are trusted by:
 Global biopharma (Amgen, Genzyme, Novo Nordisk)
 Top research institutes (Mass General, National Institutes of Health, Stanford)
 Start-up drug discovery companies (RaNA Therapeutics, aTyr Pharma, Organovo
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6. Our library of cells, tissues, and
proteomic/genomic products
 20,000+ units in stock to fill orders
quickly
 600+ product types
 Dozens of disease-specific products
All products are thoroughly tested
for quality, infectious disease, and
sterility assurance
Cells are frozen at a low passage
number
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7. Normal, non-diseased primary and
cultured cells
Prenatal and adult derived
Donor information available
Cell types across 13 organ systems
Stem Cells and Progenitor Cells
Matching fluids, sera, & plasma available
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•A2B5+ Cells
•Human Fibroblasts
•Human Renal Fibroblasts
•Human Cardiomyocytes
•Human Neural & Neural Progenitor Cells
•Human Endothelial Cells
•Human Liver Cells
•Human Chondrocytes
•CD34+ Cells
CELLULAR SYSTEMS

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DV Biologics RA fibroblasts are easily expandable to
20+ population doublings
Industry’s first commercially available biological tools
Gold Standard for disease-specific Cellular/Genomic/Proteomic Samples
Custom PREDIGREE PACKAGES: offer a unique cell panel, along with
corresponding genomic and proteomic products from an entire family

9. Proteomic and genomic tools derived
from whole tissue or culture cells
 Total RNA
 cDNA
 Lysates
 Matching FFPE and OCT blocks
We offer difficult to isolate products
from bone, brain, and cartilage
Used by scientists everywhere for:
– Gene expression studies (Microarrays)
– Disease state analysis (target
identification)
– Growth and development analysis
Cartilage Total RNA
 RNA directly from tissue
 Validation using gel and RT-PCR
 Genes expressed: aggrecan, link, MMP-13 and Col X
 Used to better understand cartilage degeneration and repair.
Figure 1: Real Time PCR was performed
using cartilage tissue cDNA, amplified using
specific primers for Collagen type X, and Aggrecan;
GAPDH was used as internal
control. Results show that Collagen type X and
Aggrecan are expressed in cartilage.
Figure 2: cDNA synthesized from cartilage
RNA (PM012-Rby reverse transcription
with random primers, and amplified by
PCR using primers specific. Markers were
expressed cartilage.
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10. Optimized maintenance, culture and
differentiation media
13 cell culture media optimized for specific
cell types
Vessel Coating and Cell Dissolution Products
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100% REPLACEMNT
GUARNATEE
(when SOPs are followed)
U-GRO HUVEC Media
•Tested and optimized
using HUVEC
•Guaranteed optimal
growth when using
AC005-F (HUVEC) Cells
•Tested for proper pH and
sterility

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PopulationDoublings
Days in Culture
Stromal Cells Growth Curve
H-Gro Leading Competitor
Out performed Industry-leader in head-to-head comparison studies
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12. Contract cellular research and custom
tissue procurement
Team of well published scientists and
state of the art scientific equipment
Tissue procurement specialists work
with a variety of hospitals and clinics
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13. Growth and maintenance of specific
cell
Analysis of gene expression patterns
Custom Cell growth optimization
Creation of genetically modified cells
for functional studies
Cell viability and purity studies
Product validation
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14. We can obtain specific and custom cells and tissues
Customer can specify cell type, donor age, or by disease
All tissue is obtained under strict IRB approval
Example: Leading Biopharma researching autoimmune
autoimmune diseases
 Donors—patients with RA, OA, and Lupus Erythematosus
 Custom collection—synovial tissue OCT blocks & FFPE blocks,
synovial cells, synovial fluid, PBMCs, serum and plasma
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15. PARTNERSHIP & COMPANY OVERVIEW

16. Company profile I
Innoprot is a spin-off of the University of the Basque Country
founded in 2007.
Develops and commercializes reagents and cell lines for high
throughput (HTS) and high content screening (HCS).
Located in Bizkaia Technology Park (Spain)
Facilities & Screening Platform:
Biosafety L2 Laboratory + Screening Laboratory
BD Pathway 855 High Content Bioimaging
Hamilton Robotics “Liquid Handling WorkStation”
Multi-Mode Microplate Reader “Synergy 2”
Technology Platform
Fluorescent Proteins (License Agreement with Evrogen)
Nomad® Biosensors Technology (Patented by Innoprot)
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17. OUR OFFER – PRIMARY CELLS
PHENOTYPIC SCREENING – PRIMARY CELLS
Single
Assays
ELISA
DEVELOPMENT
CO-CULTURE
ASSAYS
CELL
DIFERENTIATION
ASSAYS
EXPRESSION
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Phenotypic Screening
Looks at the effects that compounds induce in
cells, tissues or whole organisms
Migration, proliferation, invasion,
angiogenesis, apoptosis, vesicularization,
neurite outgrowth, etc, ….
Target-based Screening
Measures the effect of compounds on a
“specific” target protein via in vitro assays.
GPCRs, Ion channels, enzymes, kinases, etc, ..
Binding, activity, ion flux determinations,
etc…

18. OUR OFFER
TARGET-BASED SCREENING
Cell Lines for High-Throughput Screening (Label-Free)
HiTSeeker: label-free GPCRs Expressing Cell Lines
Cell Lines for Hig-Content Screening (FP tagged)
Fluorescent GPCRs Internalization Assays
NOMAD® Cell Lines (Label-Free GPCRs & Nomad Biosensor)
Fluorescent Nuclear Hormone Receptors Translocation Assays
Cell Lines for Apoptosis Assays (Oncology)
Cell Lines for targets associated to CNS Diseases
3 in vitro models for Alzheimer’s Disease (APP, tau, γ-sec.)
2 in vitro models for ALS (TDP-43 & FUS/ALS)
1 in vitro model for Parkinson’s Disease (Parkin recruit.)
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19. IN VITRO MODELS
IN VITRO DISEASE MODELS – PRIMARY CELLS
Alzheimer’s Disease Models
Neuronal Intoxication with β-amyloid peptide (1-40 or 1-42)
tau phosphorylation induced by okadaic acid
Parkinson Disease Model
DA Neurons intoxication with MPP++
Amyotrophic Lateral Sclerosis
Neural Excitotoxicity Assay induced by glutamate
Stroke Model
Neuronal Oxigen-Glucose Deprivation (OGD) Assay
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20. Alzheimer’s Disease Models
APP Processing Assay
γ-secretase activity assay
Tau Phosphorilation Assay
Parkinson’s Disease Model
Parkin Mitochondrial Recruitment Assay
α-Synuclein aggregation Assay (only service)
Amyotrophic Lateral Sclerosis (ALS)
TDP-43 Stress Granules Formation Assay
FUS/TLS Stress Granules Formation Assay
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IN VITRO MODELS
IN VITRO DISEASE MODELS I – RECOMBINANT MODELS

21. New Technology Platform: NOMAD® HCS BIOSENSORS
Currently there is no commercial platform available that
has these characteristics!
The technology measures directly the effect
of potential drugs by determining the
concentrations of the intracellular second
messengers liberated after the target
activation in living cells (cAMP, Ca++, DAG)
by fluorescent Nomad® biosensor
redistribution.
The approach does not involve the genetic
modification of any of the component of
the receptor pathway in the cell.
Robust and reproducible and could be
combined with internalization studies.
Cell lines expressing label-free GPCRs &
Nomad® Biosensor
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22. OUR OFFER – RECOMBINANT CELL LINES
NEW GENERATION OF BIOSENSORS FOR HCS - Advantages
New Fluorescent Biosensor
Adaptable to multiple HCS/HTS platforms
Image analysis (quantification of fluorescent
vesicles)
Versions for fluorescence intensity
High sensitivity in robust assays
Economic assay – no additional reagents required
Receptors are cloned without any label
Assays in living cells
Available with different colours
Different assay times (3 hours – 8 hours – 24 hours)
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23. INTRODUCTION
NOMADTM BIOSENSORS – Examples
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24. Primary Cell-Based Assays
Sampling of Available Primary Cells
Neurons
Astrocytes
Brain Microvascular Endothelial Cells
Brain Vascular Smooth Muscle Cells
Neural Precursor Cells
Dopaminergic Neuronal Precursor Cells
Schwann Cells
Brain Vascular Pericytes
A2B5+ Glial Precursor Cells
PSA-NCAM+ Neuronal Progenitor Cells
Primary Immortalized Human Microglia

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Justin Gaines
Territory Sales Manager │DV Biologics
✆ 617.347.0573
☏ 949.722.1950 x 852
✉ jgaines@dvbiologics.com