skin dermatology diagnosis investigation treatment therapy approach immunofluorescence biopsy patch test woods lamp koh dark ground hematoxylin and eosin periodic acid schiff fite farco tzanck giemsa ziehl neelsen gram staining microscopy procedure laboratory management clinical neonates examination history mechanobullous pathway pathology aetiology emmolient topical itch pruritus immunobullous disorder patient vesicobullous
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