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Universiti Brunei Darussalam 
Discovery year Internship Programme 
Presentation 
Presented by: 
Noorlela Bt Md Yassin (12B3011) 
Held in: 
Borneo Marine Research Institute 
Universiti Malaysia Sabah
Content 
• Fish Hatchery 
– Introduction 
– Organization structure 
– Areas 
• B Area 
– Introduction 
– Species 
– Hybrid species 
– Routine 
– Treatments 
– Artificial production 
• Conclusion
Fish Hatchery 
Supervisor: Dr. Faihanna Ching Abdullah
Introduction 
• Location 
Photo source: Google map
• Brief history: 
– Established in 2002 
– Designed by Prof. Dr. Shigeharu Senoo
• What? 
Place for artificial breeding, hatching and 
rearing through the early life of finfishes or shell 
fish for commercial purposes. 
• BMRI’s Fish Hatchery: 
Foundation of aquaculture program
• Hatchery Management steps: 
– Broodstock management. 
– Spawning 
– Fertilization 
– Larval and juvenile rearing 
– Sales and distribution
Organization Structure 
Prof. Dr. Shigeharu 
Senoo 
(Manager) 
Dr. Faihanna Ching 
Abdullah 
(Head of 
Aquaculture 
programme) 
Liew Chun Fong @ 
Vivian 
(Science Officer) 
Norazmi Osman 
(Senior Laboratory 
Assistant)
Areas
• Divided into different areas: 
A area
B area
L area
CD area 
Rotifer culture
CD area 
Fresh Water culture
CD area 
Aquaponic
B Area
Introduction 
• Broodstock management and juvenile rearing 
of marine fish species. 
• Consist of 6 tanks.
• Recirculating Aquaculture System (RAS). 
Culture tank Filter tank
Mechanisms of Recirculating 
Aquaculture System (RAS) 
Trash 
net 
Filter 
Filter tank Culture tank
Recirculating Aquaculture System 
(RAS) 
• Water treatment by two processes: 
– Mechanical filtration 
• By Trash net
Recirculating Aquaculture System 
(RAS) 
• Water treatment by two processes: 
– Biological filtration 
• By coral rocks
Species
Epinephelus lanceolatus (Giant grouper) 
Epinephelus fuscoguttatus (Tiger grouper)
Epinephelus coioides (Orange spotted grouper) 
Lates calcarifer (Seabass)
Cheilinus Undulatus (Napoleon Wrasse)
Hybrid Species 
• The first-in-the-world TGGG Hybrid 
– cross between Tiger grouper (E. fuscoguttatus) and 
Giant grouper (E. lanceolatus) 
• Suitable for commercial-scale production 
• Not been released in the wild. 
• Effective controls must be done to prevent their 
release into the natural environment.
Tiger grouper x Giant grouper 
(TGGG hybrid) 
Orang spotted grouper x Giant grouper 
(OGGG hybrid)
Mouse grouper x Tiger grouper 
(MGTG hybrid)
Routine 
• Cleaning trash net.
Routine 
• Feeding: 
– 3 times a week
• Diet: 
– Trash Fish 
– Fish Pellet 
– Squid 
– UMS formulated feed (Kintaro)
Routine 
• Maintenance
Treatments
• Parasite treatment: 
• Fish are badly 
infected by parasite. 
• They will under go 
fresh water 
treatment for few 
minutes.
• Buoyancy issue treatment: 
• Fish found floating upside down on surface of 
water. 
• Remove the excess air.
• Enforced feeding treatment: 
• Fish are critically ill and lose their appetite to eat 
or to weak to eat themselves 
• Use modified syringe and inject food directly into 
the fish’s stomach
Artificial production 
• Broodstock Selection: 
– Selection of male and female adult fish. 
– Criteria that they looking for: 
• Soft distended abdomen for both male and female 
• Reddish genital papillae for female 
• Pointy genital papillae for male
• Hormone treatment via injection: 
– Injected to the female fish. 
– HCG (human chorionic gonadotropin hormone) 
– Injected to either Dorsal or pectoral fins 
• Dose 500iU/Kg 
– To enhance the egg maturation.
• Stripping process: 
– Collection of egg and sperm (artificial spawning) 
Egg collection Sperm collection
• Fertilization 
– Mixing the collected eggs and sperms to allow 
fertilization. 
– Once fertilized the eggs will be transferred to L 
area to monitor the development.
Conclusion 
• Hands on experience in hatchery management 
in aquaculture industry 
– Managing fish culture 
– Handling fish 
– Learn about fish behaviour 
– Artificial production

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Fish Hatchery the B-Area

Editor's Notes

  1. Assalamualaikum and good afternoon everyone my name is noorlela and I will proceed this presentation on..
  2. BMRI’s fish hatchery the ( ) and I will present further on one of the area that have been assigned to us which is the B area ( ) and I will end it with conclusion
  3. This the fish hatchery
  4. Located near the BMRI building in UMS
  5. Just a brief history. It is established in 2002 And designed by
  6. Generally what is fish hatchery? Place for artificial breeding, hatching and rearing through the early life of finfishes or shellfish for commercial purposes. BMRI’s Fish Hatchery: Foundation of aquaculture program It provide practical site for the aquaculture students where they can obtain the necessary skills in the aquaculture industry. It only focus on fin fishes.
  7. The students are able to see and experience all the management steps that are carried out in the hatchery including: 1. Broodstock management 2. Spawning- process of releasing the eggs or sperm Natural spawning- is when the fish releases the eggs or sperm on their own. Artificial spawning- Hormone injection and let them releases their eggs or sperm on their own. Fully artificial fertilization- Hormone injection and stripping eggs or sperm 3. Fertilization 4. Larval and juvenile rearing 5. Sales and distribution
  8. The fish hatchery is run by… (nama sensei) along with
  9. Moving on to the areas in fish hatchery
  10. The fish hatchery is divided into different areas, First is the…. A Area- Culture fresh water spp B Area- Culture Marine spp L Area- for egg, larval and juvenile developmental area F Area- culture fresh water spp
  11. A Area- Culture fresh water spp B Area- Culture Marine spp L Area- for egg, larval and juvenile developmental area F Area- culture fresh water spp
  12. A Area- Culture fresh water spp B Area- Culture Marine spp L Area- for egg, larval and juvenile developmental area F Area- culture fresh water spp
  13. A Area- Culture fresh water spp B Area- Culture Marine spp L Area- for egg, larval and juvenile developmental area The CD area which include….
  14. A Area- Culture fresh water spp B Area- Culture Marine spp L Area- for egg, larval and juvenile developmental area F Area- culture fresh water spp CD area consist of rotifer culture, F. water & aquaponic
  15. A Area- Culture fresh water spp B Area- Culture Marine spp L Area- for egg, larval and juvenile developmental area F Area- culture fresh water spp CD area consist of rotifer culture, F. water & aquaponic
  16. I will discuss further on what we have done in the B Area. And the rest of the areas will be discussed by my two other colleagues.
  17. For introduction B area: B Area is the place for broodstock management and juvenile rearing of marine fish species. It consist 6 tanks (they called it the “B Tank”) Each with 4 meter wide and 3 meter deep holding 150 tonnes capacity.
  18. It applied the Recirculation Aquaculture system which consist of culture tanks and filter tanks. The filter tanks have layers of coral rocks up to 1.5m depth
  19. What is Ras? Explain on how it works: It operates by filtering water from the culture tanks using the filter tank so it can be reused within the tank. And why are they using this, is because This system reduces the amount of water and space required. The water from the culture tank will be transferred to the filter tanks. Using the “airlift” mechanisms they insert aeration inlet (here) thus the air helps to push the water up to the filter tanks. Then the filtered water will transferred back to the culture tank using the same mechanisms.
  20. Using RAS the water may be treated by two processes First is mechanical filtration by the trash net where it collects solid wastes such as faecal matter, uneaten feeds, and etc Indicate presence of eggs
  21. Second, biological filtration by coral rocks which remove dissolved toxic wastes such as ammonia.
  22. The major species that they have in B Area
  23. the hatchery has successfully produced The first-in-the-world ‘TGGG hybrid’ The hybrids have not been released in the wild. They are suitable for commercial-scale production if effective controls are in place to prevent their release into the natural environment.
  24. These are the Few example of hybrid remaining in UMS H.
  25. We also doing routine work such as.
  26. Why? Control diet Prevent obese To reduce diseases (trash fish carrier) Trash fish (Ikan baja) Low value fish (dulu) Senang di dapati
  27. And their diet are consist of Refer again on the kintaro feed
  28. Maintenance work such as making fish cages and houses and also I helped in sewing new trash net.
  29. In addition to routine work occasionally we also involve in fish treatment
  30. Such as….. Each tanks, the fish are monitored. If the fish are badly infected with parasite It will show Some of the symptoms such as Skin lesion Stress (behavior & colour) they will undergo fresh water treatment, where we will put the fish in fresh water tanks for few minutes. Once exposed to fresh water the high possibility parasite detach themselves or die. Note on the fish (high priority)
  31. The fish might have some excess air in its body cavity. It needs to be remove as it might affect the fish’s swimming pattern So we remove it by puncturing the affected part using needle.
  32. For some of the critically ill fish. Who have lose their appetite to eat or to weak to eat themselves. We will apply enforce feeding where we blend the fish pallet together with cod liver oil. T hen we will use modified syringe and inject the food directly into the fish’s stomach. This is to ensure that the sick fish is still well nourished.
  33. We are also involve in the artificial production in fish hatchery Where the process starts with the Brood stock selection soft distended abdomen for both Reddish genital papillae for female pointed
  34. Once the brood stock is chosen they will under go hormone treatment via injection
  35. After hormone treatment the fish are monitored to see the maturation of the eggs or sperm. Once matured they will under go stripping process They will press the fish abdomen to release the eggs and sperm and collect it.
  36. Once collected we will fertilized them by mixing
  37. As conclusion, working in fish hatchery give us the hands … Despite the hard work and challenging working environment, it was an enjoyable and educational experience for us. I would like to say thank you to the hatchery family for your guidance and sharing your knowledge to us.