4. Aspergillus Species in tissue
Dichotomous = branching
into two pieces
Higher magnification
4
5. Aspergillus compared to Mucormycete fungi (Mucor)
Broad hyphae rare/no septations
Broader hyphae than Aspergillus
Branching more irregular/some
at 90* angle
Thinner hyphae than Mucormycete
Regular septations, 45* angle
branching referred to as dichotomous
branching
5
19. Histoplasma capsulatum Bone marrow
lung
Yeast are usually
intracellular within
macrophages,
2-4 um in size
19
20. Histoplasma
capsulatum
In fixed tissue, P. brasiliensis forms multiple
buds on the primary yeast cell, appears similar
to macroconidia of Histoplasma
H. capsulatum
adhesive tape prep
Tuberuculate macroconidia
microconidia
**Do not confuse Histoplasma
mold phase with the yeast of
Paracoccidioides brasiliensis
complex in fixed tissue
Growth in culture
30* C for 2 weeks
Yeast of P. brasiliensis in
fixed tissue
20
21. Sporothrix schenckii
Dermis
inflammation
Yeast cells
variable in
shaper some
Cigar shaped
Asteroid body commonly
the only structure observed
Progression up lymphatics
Adhesive tape preparation /spores
form daisy head arrangement
Culture at 30* C
21
22. Penicillium (Talaromyces)
marneffei
Skin – yeast like cells
Bone
marrow
30*C culture/ note red
diffusible pigment
surrounding green colony
Adhesive prep
of mold
Skin lesions
22
27. Mycetoma
Sulfur
granule
Actinomycotic Mycetoma Eumycotic Mycetoma
Nocardia species Black (dematiacious) molds
Sulfur granule formed in tissue Sulfur granule formed in tissue
with thin filamentous bacteria with thick dark hyphae
Swollen extremity
27