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Types of Restriction Endonuclease enzymes

These are the types in the cutting of DNA fragments into pieces.

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Types of Restriction Endonuclease enzymes

  1. 1. RESTRICTION ENZYMES • Produces by bacterial cells to protect themselves against the foreign in bided DNA • There are three major activities of RE’s: 1. Recognition 2. Digestion (or) 3. Modify (Methylase) There are two types of methylase: 1. dAM (A residue) 2. dCM (C residue)
  2. 2. TYPES OF RE • Depending upon the source of isolation & the biochemical activity, RE’s are of THREE groups. [The first enzyme discovered which recognizes a given sequence is known as the prototype.] 1. Isoschizomer 2. Neoschizomer 3. Isocaudomer
  3. 3. Isoschizomer • Pairs of restriction enzymes isolated from two different sources having exactly the same recognition site and also exactly the same digestion/cleavage site. • SphI (CGTAC/G) and BbuI (CGTAC/G) are isoschizomers S1 S2
  4. 4. Neoschizomer • Pairs of restriction enzymes isolated from two different sources having exactly the same recognition site but the site of digestion/cleavage of S1 and S2 varies. • Smal(CCC/GGG) and XmaI (C/CCGGG) are neoschizomers. S1 S2
  5. 5. Isocaudomer • Pairs of restriction enzymes isolated from two different sources having different recognition site but the digestion site for S1 & S2 is exactly the same. • Produces compatible ends (having similar type of ends) • Mbo I and BamH I are isocaudomers. S1 S2

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