Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

More Related Content

You Might Also Like

Nanoerthyrosomes As Novel drug carriers

  1. 1. Srinivas Dinakar’s presentation
  2. 2. NANOERTHYROSOMES as NOVEL DRUG CARRIERS <ul><li>CO-ORDINATOR : Mrs.Pavani ,M.Pharm </li></ul><ul><li>CO-AUTHOR : Nikhil Reddy .Y </li></ul>
  3. 3. <ul><li>DEFINITION </li></ul><ul><li>Nanoerythrosomes are erythrocytes based new drug carrier which is prepared by extrusion of erythrocyte ghosts to produce small vesicles having an average diameter of 100nm </li></ul><ul><li>RESEALED ERYTHROCYTES </li></ul><ul><li>When erythrocytes are suspended in a hypnotic medium they swell about one and half times their original size and membrane ruptures resulting in the formation of pores with diameter of 200-500Å 0 </li></ul><ul><li>If the ionic strength of the medium is </li></ul><ul><li>adjusted to isotonicity and the cells are </li></ul><ul><li>incubated at 37˚and causes </li></ul><ul><li>the erythrocytes to reseal. </li></ul>
  4. 4. <ul><li>ISOLATION OF ERYTHROCYTES </li></ul><ul><li>Blood is collected in to heparinized tubes by vein puncture . </li></ul><ul><li>Blood is with drawn from cardiac or splenic puncture </li></ul><ul><li>(small animals ) and through veins (large animals) in a syringe </li></ul><ul><li>containing a drop of anti coagulant . </li></ul><ul><li>The whole blood is centrifuged at 2500rpm for 5 min at 3-5˚c </li></ul><ul><li>in refrigerated centrifuge. </li></ul><ul><li>Serum and buffy coats are carefully removed and packed cells </li></ul><ul><li>are washed with PBS. </li></ul><ul><li>The washed erythrocytes are diluted with PBS and stored at 4˚c </li></ul><ul><li>until used. </li></ul>
  5. 5. <ul><li>ERYTHROCYTES GHOST </li></ul><ul><li>RBC’s on haemolysis and washing with large volume </li></ul><ul><li>of hypnotic medium, loose nearly all their hemoglobin </li></ul><ul><li>and on releasing the resultant cells appear as pale or </li></ul><ul><li>transparent in appearance and are referred as erythrocytes </li></ul><ul><li>ghost </li></ul><ul><li>Preparation and loading of nanoerythrosomes : </li></ul><ul><li>Methods employed are: </li></ul><ul><li>Extrusion </li></ul><ul><li>Sonication </li></ul><ul><li>Electrical break down method </li></ul>
  6. 6. <ul><li>Normal erythrocytes </li></ul><ul><li>Preparation of erythrocyte ghosts </li></ul><ul><li>extrusion/ Sonication /electrical break down </li></ul><ul><li>method </li></ul><ul><li>Nano vesicles </li></ul><ul><li>staining with 0.1% uranyl acetate and </li></ul><ul><li>microscopical examination </li></ul><ul><li>Nanoerythrosomes </li></ul>
  7. 7. <ul><li>EXTRUSION </li></ul><ul><li>Erythrocytes ghosts suspension was </li></ul><ul><li>extruded through polycarbonate membrane filter </li></ul><ul><li>which was connected to an adapter </li></ul><ul><li>Nanoerythrocytes were obtained by </li></ul><ul><li>8 consecutive extrusions </li></ul><ul><li>Extrusion was done using nitrogen </li></ul><ul><li>pressure </li></ul>
  8. 8. <ul><li>SONICATION </li></ul><ul><li>Erythrocytes ghosts suspension was </li></ul><ul><li>sonicated at an energy level of 50W </li></ul><ul><li>for 5 min using dismembrator. </li></ul><ul><li>ELECTRIC BREAK DOWN </li></ul><ul><li>Erythrocyte ghost suspension was subjected to </li></ul><ul><li>variable electric voltage for 100 µseconds. </li></ul><ul><li>Temperature maintained at 37˚c </li></ul><ul><li>In extrusion and Sonication the final </li></ul><ul><li>preparation was stored at 3-5 ˚c in refrigerator </li></ul>
  9. 9. <ul><li>DRUG NANOERYTHROSOME CONJUGATION </li></ul><ul><li>Methotrexate was conjugated to nanoerythrosomes membrane using glyceraldehydes as cross linker. </li></ul><ul><li>The complex was washed with PBS and stored at 3-5˚c </li></ul><ul><li>Entrapment efficiency </li></ul><ul><li>Amount of drug loaded x 100 </li></ul><ul><li>Amount of drug added </li></ul>
  10. 10. IN VITRO CHARACTERIZATION <ul><li>SCANNING ELECTRON MICROSCOPY OF nEs </li></ul><ul><li>EG’s (2parts) + saline buffer (25 parts) </li></ul><ul><li>Formulation is dehydrated and dried to critical point </li></ul><ul><li>One drop of the diluted mixture is placed on a silver </li></ul><ul><li>coated copper grid ,then coated with gold </li></ul>
  11. 11. TRANSMISSION ELECTRON MICROSCOPY OF nEs <ul><li>EG’s (2parts) + saline buffer(25parts) </li></ul><ul><li>One drop of the diluted mixture is placed on a coated copper </li></ul><ul><li>grid and negatively stained with 1% uranyl acetate </li></ul><ul><li>Then the drop of mixture is seen through a transmission </li></ul><ul><li>electron microscope. </li></ul>
  12. 12. <ul><li>ROUTE OF ADMINISTRATION </li></ul><ul><li>They can be administered intravenously or intra arterially. </li></ul><ul><li>APPLICATIONS </li></ul><ul><li>They can target the drugs with in reticulo endothelial system. </li></ul><ul><li>Carriers for drugs , proteins , enzymes ,macromolecules. </li></ul><ul><li>Treatment of liver tumors, parasitic diseases, enzyme deficiency. </li></ul><ul><li>FUTURE PERSPECTIVES </li></ul><ul><li>A large amount of valuable work is needed so as to utilize the potentials of nanoerythrosomes </li></ul><ul><li>Disease like cancer could surely find its cure with the help of nanoerythrosomes. </li></ul><ul><li>Genetic engineering aspects can be coupled to give newer dimension to the existing drug carrier concept. </li></ul>
  13. 13. <ul><li>CONCLUSION </li></ul><ul><li>‘ GOLDEN EGGS IN NOVEL DRUG DELIVERY SYSTEM’ considering </li></ul><ul><li>their tremendous potential </li></ul><ul><li>REFERENCES </li></ul><ul><li>Advances in controlled and novel drug delivery ,edited by N.K JAIN ,CBS publishers.pg.no 332-351 </li></ul><ul><li>Anatomy and Physiology </li></ul><ul><li>Ross and Wilson’s Human Anatomy and Physiology </li></ul>

×