Germplasm conservation is the most successful technique to conserve the genetic traits of endangered and commercially valuable species.

1. GERMPLASM
CONSERVATION
OF PLANTS
PRESENT BY:
MISS.VANITA BHAGAT
M.SC. PART II (SEM III)
(DEPARTMENT OF
BIOTECHNOLOGY)
ST.FRANCIS DE SALES COLLEGE,
SEMINARY HILLS, NAGPUR.
SUPERVISED BY:
MISS. PALLAVI G. SHIWANKAR

2. CONTENTS
• Introduction
• Need of germplasm conservation
• Strategy of Germplasm Conservation
• Biotechnology in Germplasm
Conservation
• Advantages
• Limitation
• References

3. INTRODUCTION OF GERMPLASM
• Collection of genetic resources.
•Germplasm is a living tissues from
which new plants can be grown. It
can be a new seed or another plant
part-a leaf, a piece of stem, pollen or
even just a few cells that can be
turned into the whole new plant.

4. NEED FOR GERMPLASM
CONSERVATION

5. Conserve the
genetic traits
Live information
source

6. STRATEGY OF GERMPLASM
CONSERVATION
• Conservation in the natural environment by
establishing biosphere reserves.
In situ
• The technique of conservation of all levels of
biological diversity outside their natural
habitats through different techniques like
botanical garden, and gene bank.
Ex situ

7. IN SITU CONSERVATION

8. Examples
Natural
reserves
Biosphere
reserves
National
parks
Marine
parks

9. EX SITU CONSERVATION

10. Seed gene
bank
Pollen
storage
DNA
storage
In vitro
storage Methods

11. Short term
storage or in
vitro method
Long term
storage or
cryopreservation
BIOTECHNOLOGY IN GERMPLASM
CONSERVATION

12. LONG TERM STORAGE OR
CRYOPRESERVATION
Cryopreservation (-196℃ )
obtained from liquid nitrogen
Liquid nitrogen, cell stay in a
complete inactive state

13. TECHNIQUES OF
CRYOPRESERVATION

14. Selection of
plant
materials
Pre freezing
treatment
Desiccation
Vitrification Cryoprotectants
Freezing
Storage
Thawing Viability
Regeneration

15. Several species can be cryopreserved.
Plant material maintained for several
year, and it can be used when needed.
Ideal method for long term conservation
of cell culture.
Diseases free plant material can be frozen
and propagated whenever required.
Plant material from endangered species
can be conserved.
A
D
V
A
N
T
A
G
E
S

16. Greater skill in handling and maintainance of
culture required.
Sophisticated facilities are required.
Cell/tissues get damage during cryopreservation.
Cost of maintenance of large collection is very
high.
L
I
M
I
T
A
T
I
O
N
L
I
M
I
T
A
T
I
O
N

17. REFERENCES
• Rajasekharan, PE & Sahijram, Leela. (2015). In Vitro Conservation of Plant Germplasm.
10.1007/978-81-322-2283.
• Engelmann, F. (1991). Tropical plant germplasm conservation.
• C. M. Govil. “Plant Biotechnology and Genetic Engineering.” 418-427.
• H.S.Chawala.(2002).“Introduction of Plant Biotechnology.”10.1201/97
81315275369.Third Edition.146-153.
• Taiz & Zeiger. “Plant Physiology.” ( Third Edition).
• https://biologyboom.com/germplasm-conservation/ (assessed on 6/9/2019).
• http://www.biologydiscussion.com/biotechnology/germplasm-conservation/germplasm-
conservation-and-cryopreservation-with-diagram/10757 (assessed on 5/9/2019).