3. Comparison of the Pulmonary & Systemic Circulation
PULMONARY
CIRCULATION
LOW PRESSURE
- because it only needs to pump blood to
the top of the lungs.
- if it is HI pressure, then following
Starling forces, the fluid would flood the
lungs.
LOW RESISTANCE
- only 1/10th of the resistance of the
systemic circ.
- arterioles have less smooth muscle, veins
are wider & shorter
& pulmonary vessel walls are thinner.
HIGH COMPLIANCE
- accommodates 5 L of blood (same as the
systemic circulation)
- Accommodates shifts of blood more
quickly e.g. when a person shifts from a
standing to a lying position
SYSTEMIC CIRCULATION
HIGH PRESSURE
- B/c it needs to send blood to
the brain even when standing & to
the tip of en elevated fingertip.
HIGH RESISTANCE
- because of increased
smooth muscle in the arterioles
& the arterial capillary
LOW COMPLIANCE
- because of resistance
offered by the arterioles and
the metarterioles.
4. Pulmonary Circulation
P. circulation contain 10% of total blood volume but can easily
altered as much as 50% which is seen in..
-ve pressure breathing
Supine pressure as compared with up right position
LVF
Any cause of Sys vasoconstriction
Over transfusion
5. PRESSURES IN THE PULMONARY
SYSTEM
Right Ventricle:
Systolic= 25 mmHg
Diastolic= 0-1 mmHg,
Pulmonary artery:
Systolic= 25 mmHg(20-30)
Diastolic= 8 mmHg(8-12)
Mean Pulmonary arterial pressure= 15 mmHg(12-15)
Pulmonary Vein:
Averages about 5 mmHg
Pulmonary capillaries:
7 mm Hg
Left atrium:
Mean left atrial pressure 8-15mmHg
6.
7. Measurement
of pulmonary
blood flow :
FOUR
METHOD
Radio active gases1.
Direct Fick method2.
Indicator –Dilution Method3.
Body Plethysmography(to measure pul
capillary flow)
4.
8. HYPOXIC pulmonary vasoconstriction (HPV) is a
reflex contraction of vascular smooth muscle small
pulmonary arteries in the pulmonary circulation in
response to low regional partial pressure of oxygen
(PO2).
Hypoxic pulmonary
vasoconstriction
( Euler-Liljestrand mechanism)
9. HPV:-
Most of response occur locally
Due to this reflex blood diverted in pulmonary circulation from
less perfuse or collapse area to well perfused area to maintain
arterial saturation
In normal person 50% of total shift occur with in 2 min and
complete after 7 min after occurrence of alveolar hypoxia.
Reflex accentuated by H+ ions concentration.
10. The mechanism of
hypoxic pulmonary
vasoconstriction.
Hypoxia causes closure
of voltage-gated
potassium channel,
leading to K+
accumulation
intracellularly. It leads
to depolarization of the
cells, opening of
voltage-gated calcium
channel and calcium-
mediated pulmonary
vasoconstriction
11. Effects of anesthetics
The general view is that the inhalational agents inhibit HPV and
intravenous agents do not.
The studies done on the same, none are with consistent results.
But it is evident from these studies that both inhalational and intravenous
agents inhibit, but inhibition is less in intravenous agents than in
inhalational.
Effect of Halothane on HPV is conflicting
12. Other factors
Inhibition:
Trauma, vasodialator drugs( nitroprusside, nitroglycerin, nitric oxide,
isoprenaline).
Vasoconstrictors: which constrict the pulmonary vasculature
(noradr, dopamine, histamine) reduces the effectiveness of HPV.
Indirect inhibitors: mitral stenosis, volume overload, hypothermia,
thromboembolism, large hypoxic lung segment.
14. Lungs supplied by:-
Two Circulatory channels:-
1. Pulmonary vessels(convey deO2 blood to alveolar wall and drain
O2 blood to left side of heart )
The Pulmonary artery (Intrasegmental)emerges from the Right
Ventricle, follows the bronchial tree, bifurcates with it and enters the lung.
When it reaches the alveoli, it forms a dense network of capillaries like a
flowing sheet surrounding the alveoli so that efficient oxygenation of the
blood can take place.
The veins that arise carry the oxygenated blood and form the Pulmonary
Veins (intersegmental)that take the blood to the Left Atrium.
Pul. Veins use as a landmark to resect a particular segment of lung
15. 2.Bronchial vessels-
Bronchial artery :-supplies the lung parenchyma, bronchial glands and
tissue.
Rt side :- 1 BA arise indirectly from DTA(aorta) either from 3rd
posterior intercostal artery(mostly)
Lf side :- 2 BA arise directly from DTA(aorta)
Bronchial veins:-Don’t drain all blood supplied by P. arteries some drain
into P. veins.
Superficial Bronchial Veins:-
RT side:- drain in Azygous Vein
LF side :- drain in superior intercostal or ass. Hemi azygous veins
Deep Bronchial Veins:-
Aries as intra pulmonary bronchial plexus and end in main P. vein or Rt
atrium
16. LUNG RECEIVES 2 BLOOD SUPPLIES
From the Left
Ventricle
BRONCHIAL
ARTERIES
Carry oxygenated blood
Blood supplied to the
conducting airways,
lung interstitium &
tissue.
From the Right
ventricle
PULMONARY
ARTERIES
Carry deoxygenated mixed -
venous blood
Blood circulates the
alveoli to get
oxygenated.
19. KEY
POINTS
ALVEOLAR VENTILATION–
(VA)
ALVEOLAR PERFUSION-
PULMONARY CIRCULATION
(Q)
VENTILATION – PERFUSION
RATIO (VA/Q)
VENTILATION PERFUSION
MISMATCH
SHUNT
DEAD SPACE
20. Normal alveolar ventilation(VA)=4lit/min
Normal total perfusion=5lit/min.
V/Q=0.8
At the base of lung .Blood Flow > Ventilation. V/Q=0.63 because
ventilation is proportionally low. S/O Shunted Blood
At the top of lung. Blood Flow <Ventilation V/Q=3.3 because here
perfusion is about nil. S/O Dead space ventilation
Ventilation and perfusion are both missing
S/O Silent Unit
REALTION BETWEEN VENTILATION
AND PERFUSION IN LUNG
21. Def:- Usually measured as a sum of all exhaled gas
volume in one min.(minute ventilation)
Minute Ventilation V = RR x VT
Volume of the inspired gas participating in alveolar gas exchange
/minute is called ALVEOLAR VENTILATION-VA
VA = RR x VT-VD
Not all inspired gas participating in alveolar gas exchange
DEAD SPACE – VD
Ventilation
22. Ventilation
Dead space ventilation - wasted ventilation
ventilation of unperfused alveoli
Dead space VD = 2ml/kg (Normally 150)
Dead space ratio VD/ VT = 33%
{BOHR EQUATION}
VD = PACO2 – PECO2
VT PACO2
VD = Dead space
VT = Tidal volume
PaCO2 = Arterial CO2
PETCO2 = End tidal CO2
USE FOR:-approximate alveolar concentration and Co2
tension in expired gases.
23. Ventilation is unevenly distributed in the lungs.
Rt lung more ventilated than Lt lung [53% & 47%]
Due to gravitational influence on intra plural pr [decreased
1cm/H2O per 3cm decrease in lung height] lower zones
better ventilated
25. Ventilation pattern - VA
•Pleural pressure [Ppl]
increased towards lower zone
•Constricted alveoli in lower
zones & distended alveoli in
upper zones
•More compliant alveoli towards
lower zone
•Ventilation: distributed more
towards lower zone
26. •Upper zone:
less pleural pressure,
distended more
& hence less compliant
•Lower zone:
more pleural pressure,
less distended,
& hence more compliant
Ventilation pattern - VA
27. Pulmonary Perfusion
Due to gravitational influence the lower – dependent areas
receive more blood
Pulmonary blood flow 5l/min
Total pulmonary blood volume -500ml to 1000ml
These volume going to be spreaded all along the alveolar
capillary membrane which has 50 to 100 m² surface area
28. ZONE-I: Only exist if Ppa very low in
hypovolemia / PA in PEEP
ZONE-II: Perfusion α Ppa-PA
arterial-alveolar gradient
ZONE-III: Perfusion α Ppa-Ppv
arterial-venous gradient
ZONE-IV: Perfusion α Ppa-Pist
arterial-interstitial gradient
Pulmonary circulation – Alveolar Perfusion Q
30. Respiratory Quotient
The relationship between the oxygen consumption and the carbon dioxide
production is the Respiratory Quotient (RQ).
Normal VCO2: 200 ml/min
Normal VO2: 250 ml/min
RQ is VCO2/VO2
(200 ml/min)/(250 ml/min) or 0.8
31.
32. Pathophysiology of the ZONES of
the Lungs
Effect of Exercise:
The blood flow in all parts of the lungs may increase during exercise. In the
top of the lungs, the increase may be 700-800% while in the lower parts
may be 200-300%. This is because during exercise pulmonary vascular
pressures rise enough during the exercise to convert the lung apices from
zone 2 to zone 3 pattern of flow.
Zone 1 Blood flow occurs only during Abnormal conditions.
Usually this is not seen unless there are 2 condition:
- if an upright person is breathing against a positive air pressure and
the intra-alveolar air pressure is higher than normal.
- in an upright person, pulmonary circulatory pressure is very low as
seen after severe blood loss.
33. MEANS – WASTED PERFUSION
SHUNT – 1. ABSOLUTE SHUNT : ANATOMICAL SHUNTS – V/Q = 0
2. RELATIVE SHUNT : UNDER VENTILATED LUNGS –V/Q ≤ 1
SHUNT ESTIMATED AS VENOUS ADMIXTURE
VENOUS ADMIXTURE EXPRESSED AS A FRACTION OF TOTAL
CARDIAC OUTPUT QS/QT
QS = CCO2-CAO2
QT CCO2-CVO2
NORMAL SHUNT- PHYSIOLOGIC SHUNT < 5%(NORMAL SHUNT: 3 TO
5% • SHUNTS ABOVE 15% ARE ASSOCIATED WITH SIGNIFICANT
HYPOXEMIA)
Q
V
V/Q<1
SHUNT
34. Anatomical shunt-true shunt
Pathological shunt
Physiological shunt
Atelectic shunt
TYPE OF SHUNT
35.
36.
37. • SHUNTS have different effects on arterial PCO2 (PaCO2 ) than on arterial PO2 (PaO2
).
• Blood passing through under ventilated alveoli tends to retain its CO2 and does not
take up enough O2.
• Blood traversing over ventilated alveoli gives off an excessive amount of CO2, but
cannot take up increased amount of O2 because of the shape of the oxygen-
hemoglobin (oxy-Hb) dissociation curve.
• Hence, a lung with uneven V̇ /q relationships can eliminate CO2 from the over
ventilated alveoli to compensate for the under ventilated alveoli.
• Thus, with Shunt, PACO2 -to-PaCO2 gradients are small, and PAO2 -to-PaO2
gradients are usually large.
SHUNT
40. QUANTIFICATION - SHUNT
1. SHUNT RATIO QS = CCO2-CAO2
QT CCO2-CVO2
• CAO2 = O2 CARRIED BY HB + DISSOLVED O2 IN PLASMA
= 1.34 X HB% X SAO2 + 0.003 X PAO2
•CcO2-Pulmonary end capillary O2
content
•CaO2-Arterial O2 content
•CvO2-Mixed venous O2 content
41. DEAD SPACE ( Wasted Ventilation)
Part of respiratory system which is not taking part in alveolar gas
exchange K/as DEAD SPACE (Vd)
TWO TYPE:-
1.Physiological dead space OR Function dead space
Some gas remains in the non respiratory airways
ANATOMIC DEAD SPACE
Some gas in the non per fused /low per fused alveoli
PHYSIOLOGIC DEAD SPACE
2. Apparatus Dead space
42. Physiological Dead space
Normal :- 2 ml/kg
In Intubated pt :- 1.25 ml/kg
Vd(ana)=Vd(phy) –Vd(alv)
Due to Exercise (increase CO) –decrease Vd(phy)-D/t decrease in
Vd(alv)
43. Factor affecting Vd(ana)
1. Age and Sex: at infancy 3.3ml/kg and Old age higher
male >female(can be up to 100)
avg 2.2ml/kg
2. Depression of lower jaw and flexion of Head : Decreasd by 30 ml
3. Protrusion of jaw and extension of Head: Increased by 40 ml
4. Intubation reduce : 70 ml and tracheostomy 90ml
5. Pneumaectomy ↓
6. Decreased TV
44. Factor affecting Vd(alv)
INCRESE BY
1. IPPV
2. Lateral posture in mechanically ventilated patient
3. Decrease pulmonary perfusion
4. Improper Ventilator Setting
Large TV
High Ventilatory Rate
Reduce inspiratory time
45. 2. Apparatus Dead Space
D/t Use of Anaesthetic mask and anaesthetic circuit added in Conduction
zone
Avg mask and circuit volume up to expiatory valve 125 ml .
So always add this dead space to achieve desired TV.
46. DEAD
SPACE
DEAD SPACE ESTIMATED AS RATIO VD/VT
1. (BOHR EQ.) VD = PACO2-PECO2
VT PACO2
NORMAL DEAD SPACE RATIO < 33%(20-40%)
DRING ANAESTHESIA WITH PASSIVE VENTILATION VD(PHY)
CAN BE ROUGHFLY CALCULATED BY COOPER’S FORMULA:-
VD/VT=33+AGE/3 %
Q
V
V/Q= ∞
48. General consideration
Ambient Air
O2 = 20.93% = ~ 159 mm Hg PO2
CO2 = 0.03% = ~ 0.23 mm Hg PCO2
N2 = 79.04% = ~ 600 mm Hg PN2
Tracheal Air
Water vapor reduces the PO2 in the trachea about 10 mm
Hg to 149 mm Hg.
Alveolar Air
Alveolar air is altered by entry of CO2.
Average alveolar PO2 = 103 mm Hg
49. Solubility
CO2 is about 25 times more soluble than O2.
CO2 and O2 are both more soluble than N2
Gas Exchange in the Lungs
PO2 in alveoli ~ 100 mm Hg
PO2 in pulmonary capillaries ~ 40 mm Hg
Result: O2 moves into pulmonary capillaries
PCO2 in pulmonary capillaries ~ 46 mm Hg
Average arterial blood gases equal
PO2 100 mm Hg
PCO2 40 mm Hg
50. *
Concentration and Partial Pressure of Respired
Gases
Partial pressure = Percentage of concentration of specific gas ×
Total pressure of a gas
Dalton’s law
Total pressure = Sum of partial pressure of all gases in a mixture
Movement of Gas in Air and Fluids
Henry’s law
Gases diffuse from high pressure to low pressure.
Diffusion rate depends upon
Pressure differential
Solubility of the gas in the fluid
51. Pressure Differential
The difference in the pressure of specific gases from the capillary blood to
the alveoli dictates the direction of diffusion.
52. *
Physical Principles of Gas Exchange
Diffusion of gases through the respiratory membrane
Depends on membrane’s thickness, the diffusion
coefficient of gas, surface areas of membrane, partial
pressure of gases in alveoli and blood
Relationship between ventilation and pulmonary capillary flow
Increased ventilation or increased pulmonary capillary
blood flow increases gas exchange
Physiologic shunt is deoxygenated blood returning
from lungs
53. Gas Exchange
External Respiration
The exchange of gases
Diffusion between the alveolar air and pulmonary capillary blood
Driven by
partial pressure (P) gradients for O2 and CO2
Solubility of gas which is affected by
Pressure gradient
Solubility coefficient for the particular gas
Temperature
Given the same pressure gradients and temp, O2 will reach equilibrium at
a lower dissolved content than will CO2…. Why?
54. The exchange membrane components and organization
Gas Exchange
External Respiration
58. Gas Exchange
What happens when alveolar PO2 drops?
Solubility rules indicate that
If PO2 drops, then the amount dissolved in blood also drops!
Creating a hypoxic condition
Factors that may cause low arterial PO2
1. Not enough O2 reaching alveoli
2. Exchange between alveoli and pulmonary capillaries has a problem
3. Not enough O2 transported in blood
59. Gas Exchange
Hypoxia classifications
Hypoxic
hypoxia
Low arterial PO2
altitude, hypoventilation, lung
diffusion capacity, altered
ventilation-perfusion ratio, asthma
Ischemic
hypoxia
Hypoxia from
reduction in blood
flow
heart failure (systemic anemia),
shock (peripheral hypoxia),
thrombosis (single organ hypoxia)
Anemic
hypoxia
Total O2 bound to
Hb
hemorrhage, low Hb, CO
poisoning, altered Hb binding
Histotoxic
hypoxia
cells being poisoned,
and can’t use O2
Cyanide, H2S, alcohol, narcotics
60. Gas Exchange
Hypoxia Problems
1. Not enough O2 in alveoli…
High elevation
What about top of Mt. Everest at 29,029 ft Atmospheric
pressure = 30kPa or 225 mm Hg
PO2 then must be 47.25 mm Hg
A nearly 71% decrease in available oxygen in the blood!
To compensate ventilations increase from 15 per minute
to between 80-90 ventilations per minute
61. Gas Exchange
Hypoxia Problems
2. Interference with alveolar capillary
exchange
Alveolar air is normal but the exchange
isn’t
Caused by
Less surface area for exchange (b)
Increased thickness of alveolar
membrane (c)
Increased distance between alveolar
membrane and capillary membrane (d)
63. 23-
63
Oxygen and Carbon Dioxide
Diffusion Gradients
Oxygen
Moves from alveoli into
blood. Blood is almost
completely saturated with
oxygen when it leaves
the capillary
P02 in blood decreases
because of mixing with
deoxygenated blood
Oxygen moves from
tissue capillaries into the
tissues
Carbon dioxide
Moves from tissues
into tissue capillaries
Moves from
pulmonary capillaries
into the alveoli
66. Gas Transport
General Process
Oxygen once in blood
will
A. remain as dissolved
oxygen
B. Bind to hemoglobin
(Hb) to make HbO2
67. Gas Transport
General Process
TOTAL blood O2 content = quantity dissolved in plasma +
amount bound to Hb (HbO2)
Why have hemoglobin?
To ensure enough systemic O2!
Dissolved oxygen content in blood volume
15 ml O2/min reaching the systemic tissues
O2 requirement at rest = ~250 ml O2/min
Oxygen bound to hemoglobin, allows
the total amount of oxygen in the blood
to exceed 250 ml O2/min
69. Gas Transport
General Process
O2 in blood quickly associates with hemoglobin (Hb),
forming oxyhemoglobin (HbO2)
allows for blood to carry an extra
985 ml of oxygen/min in an average
blood volume of 5L
Dissolved = 15ml O2/min transported
Associated with Hb = 985 ml O2/min
Total Oxygen carrying capacity =
1000ml/min or 1L/min
4x’s greater than “at rest” demand
70. OXYGEN DELIVERY IN CRITICAL
ILLNESS
• Tissue hypoxia is due to disordered regional
distribution of blood flow • often caused by
capillary microthrombosis after endothelial
damage and neutrophil activation rather than
by arterial hypoxaemia OXYGEN STORES
• o2 stores are limited to lung and blood. • The amount of O2
in the lung is dependent on the FRC and the alveolar
concentration of oxygen.
• Breathing 100% oxygen causes a large increase in the total
stores as the FRC fills with oxygen • This is the reason why
pre-oxygenation is so effective.
71. THE EFFECTS OF ANAESTHESIA
• The normal protective response to hypoxia is reduced by anaesthetic drugs and this effect extends into the post-
operative period.
Following induction of anaesthesia :
• FRC ↓
• V/Q mismatch is ↑ed
• Atelectasis develops rapidly
• This 'venous admixture' increases from N 1% to around 10% following induction of anaesthesia.
Volatile anaesthetic agents suppress hypoxic pulmonary vasoconstriction.
• Many anaesthetic agents depress CO and therefore ↓ O2 delivery.
• Anaesthesia causes a 15% ↓ in metabolic rate and therefore a reduction in oxygen requirements.
• Artificial ventilation causes a further 6% ↓ in oxygen requirements as the work of breathing is removed.
72. Gas Transport
Carbon Dioxide
Why be concerned with CO2 transport?
Transported three ways:
Dissolved in blood (~7%)
Converted to bicarbonate ions (~70%)
Attaches to Hb (~23%)
CO2 + Hb ↔ Hb·CO2(carbaminohemoglobin)
73. INTRODUCTION TO PHSYIOLOGY OF CO2
TRANSPORT
• end-product of aerobic metabolism. – production averages
200 ml/min in resting adult
– During exercise this amount may increase 6x
• Produced almost entirely in the mitochondria.
• Importance of co2 elimination lies in the fact that -Ventilatory
control system is more responsive to PaCO2 changes.
74. Dissolved carbon dioxide
• Carbon dioxide is 20 times more soluble than oxygen;
• obeys HENRY’S LAW, which states that the number of molecules in solution is
proportional to the partial pressure at the liquid surface.
PCO2 x S = CO2 conc in sol
S = Solubility Coefficient
Value dependant upon temp (inversely proportional) more temp lesser amount of CO2
dissolved.
• The carbon dioxide solubility coefficient is 0.69 ml/L/mm Hg at 37C.
• In absolute terms only 0.3 ml of CO2/dL transported in dissolved form
• During heavy exercise contribution of dissolved CO2 can 7 fold 1/3 of total
CO2 exchange
75. CO2 Transport as Bicarbonate
CO2 in solution combines with water to form carbonic acid.
Carbonic anhydrase
Zinc-containing enzyme within red blood cell
Carbonic acid ionizes into hydrogen ions and bicarbonate ions.
76. CO2 BOUND AS HCO3
•Dissolved CO2 in blood reacts with water to form
Carbonic Acid
•CO2 + H2O H2CO3
Carbonic acid dissociates into H+ & HCO3
When conc of these ions increase in RBCs, HCO3
diffuses out
but H+ can’t easily do this because cell
memb is relatively impermeable to cations.
Thus to maintain electrical neutrality, Cl ions move into
cell from plasma CHLORIDE SHIFT
77. CO2 Transport as Carbamino
Compounds
CO2 reacts directly with amino acid to form carbamino compounds.
Haldane Effect: Hb interaction with O2 reduces its ability to combine with
CO2.
This aids in releasing CO2 in the lungs.
78.
79.
80.
81.
82. Gas Transport
Carbon Dioxide
Important things to
consider!
1. The H+ created
during
bicarbonate ion
formation
2. The transport of
HCO3
- out of the
cell occurs with
the movement of
Cl-
into the cell
called the
chloride shift
Both must
reverse in the
lungs!
84. OXYGEN CARRIAGE BY BLOOD
In two ways
Dissolved in plasma.(5%)
Combination with Hb(95%)
85. Hemoglobin
Why is hemoglobin so effective?
Each subunit of the quaternary
structure has a binding site for
oxygen
The heme group of each subunit
contains a prophyrin ring with an iron
atom (Fe2+) a the center
This Fe2+ reversibly binds O2 in
accordance with the law of mass action
Typically PO2 drives this reaction
86. Gas Transport
Hemoglobin
Hb structure can vary
Adult Hb
The subunits are alpha, beta, gamma, delta
Most common arrangement is 2 alpha, and 2 beta units
(HbA) >95%
Also some where:
2 alpha & 2 delta subunits present (HbA2) ~2.5%
2 alpha & 2 gamma subunits present (HbF) rare
Fetal Hb (HbF)
Gamma chains in place of the beta chains.
Creates Hb molecules with a higher affinity for oxygen
Hydroxyurea treatment in adults with sickle cell anemia
stimulates development of more HbF than HbA
87. OXYHEMOGLOBIN
One Hb molecule with its 4 heme group is capable of binding 4
molecules of O2.
1gm of fully oxygenated Hb contains 1.34ml of O2 (vary depending
on Fe content)
At an arterial PO2 of 100mmHg,Hb is 98% saturated,thus 15gm of
Hb in 100ml blood will carry about 20ml of O2
=1.34ml x 15gm x 98/100=20
88. DISSOLVED OXYGEN
• Henry’s law :states that the concentration of any gas in a
solution is proportional to its partial pressure
• Gas concentration= x partial pressure
is the gas solubility coefficient
=0.003ml/dl(100ml of blood)/mmHg for O2
• Dissolved O2 in arterial blood is thus 0.3ml/dl (0.003ml/dl
x100mmHg).
89. Venous blood have an O2 partial pressure of 40mmHg and Hb is
75% saturated.thus it contains about 15ml of O2/100ml
1.34x15x75/100=15
Thus every 100ml of blood passing through the lungs will take up
5ml of O2
90. OXYGEN
CONTEN
T
Total O2 content of blood is the sum of O2 in the
solution & that carried by Hb.
O2 content
91. OXYGEN FLUX
Amount of O2 leaving the left ventricle per minute in the arterial blood .
O2 content of arterial blood X cardiac output
O2 content of arterial blood = (O2 bound to Hb + dissolved O2)
i.e 20ml+0.3ml=20.3ml/dl(20.3ml/100ml)
So O2 flux=20.3ml/100ml X 5000ml=1000ml
93. Relates saturation of Hemoglobin (Y axis) to
partial pressure of O2 (X axis)
It’s a sigmoid shaped curve with a steep lower
portion and flat upper portion
Describes the nonlinear tendency for O2 to bind
to Hb.
O2 DISSOCIATION CURVE(ODC)
94.
95. Ferrous iron in each heme binds with one O2
One Hb molecule can bind 4 molecules of O2
Deoxy Hb : globin units are tightly bound in a tense configuration
(T state)
As first molecule of O2 binds, it goes into a relaxed configuration
(R state) thus exposing more O2 binding sites 500 times
increase in 02 affinity characteristic sigmoid shape of ODC
97. SHAPE OF THE CURVE
Characteristic sigmoid shape which offers many physiological advantages
It reflects the physiological adaptation of Hb to take up O2 at higher partial
pressures (alveoli) and release oxygen at lower partial pressures (tissues )
98. The flat upper portion means that even if PO2 falls somewhat,
loading of O2 wont be affected much.
Even when red cells take up most of the O2 from alveoli , PO2 drop
is less compared to gain in saturation a large PO2 difference still
exists for diffusion of O2 to continue
The steep lower part of the curve means peripheral tissues can
withdraw large amounts of 02 for only a small drop in capillary PO2.
This maintenance of blood PO2 assists diffusion of 02 into tissue
cells
99. The characteristic points on the curve are:
1) The arterial point
PO2=100mmHg and SO2=97.5%
2) The mixed venous point
PO2=40mmHg and SO2=75%
3) The P50
PO2=27mmHg and SO2=50%
ANCHOR POINTS IN THE CURVE
100.
101. The oxygen saturation curve for fetal
hemoglobin (blue) appears left-shifted when
compared to adult hemoglobin (red) since fetal
hemoglobin has a greater affinity for oxygen
102. It is the partial pressure at which 50% of Hb is saturated.
At a pH of 7.4 , temp 37C , the PO2 at which the Hb is 50%
saturated (P50) is 27mmHg
When affinity of Hb for 02 is increased , P50 decreases : shift to left
in ODC
When affinity is reduced , P50 increases : shift to right in ODC
P50
105. Right shift - High P50 (>27mmHg)
Hb has decreased affinity for O2
O2 delivery facilitated at tissue level
Causes:
Increase in H+
Increase in temperature
Increase in 2,3 DPG
Increase in PCO2
Exercise
Anaemia
Drugs : propranalol , digoxin etc
106. Left shift - Low P50 (<27mmHg)
Hb has ↑ed affinity for O2
O2 delivery at tissues is decreased
Causes:
Low H+
Low temperature
Low 2,3 DPG
Low PCO2
Variants of normal Hb (fetal Hb, carboxy Hb, met Hb)
107. Temperature
Increase in temperature decreases Hb-O2 affinity and curve is
shifted to right
Decrease in temperature increases affinity and curve shifted to left
decreased release of O2
But this wont cause hypoxia because in hypothermia body O2
demand is also less
FACTORS AFFECTING ODC
108. Hydrogen ions
Acidosis decreases Hb-O2 affinity and curve is shifted to right
Deoxy Hb binds with H+ more actively than does oxy Hb
H+
+ HbO2 H.Hb +O2
Advantageous at tissue level
109. Carbon dioxide
Effects attributed to changes in pH
CO2 + H2O H2CO3 H + HCO3
Increase in CO2 shifts curve to right causing more release of O2
BOHR EFFECT
110.
111. 2,3 DPG
Produced in red cells by Embden meyerhof shunt pathway of
glycolysis
Normal concentration : 4mmol/l
Binds to deoxyHb and reduces its affinity for O2
ODC is shifted to right
Fetal erythrocytes have lower concn of 2,3 DPG and hence HbF has
a higher affinity for O2
113. FACTORS DECREASING 2,3 DPG
Polycythemia
Hyperoxia
Chronic alkalosis
Hypothyroidism
Blood storage
NB: blood stored with ACD anticoagulant loses 2,3 DPG faster (6-7
days) than CPD blood. Effect starts immediately after transfusion
and may last for 2-3 days
114. Physiological situations
(1) Exercise
ODC for skeletal muscles shifted to right
This ensures max O2 delivery for exercising muscles
Factors : Increased CO2 production
Increased Temperature
Presence of myoglobin
(higher O2 affnity)
Other conditions affecting ODC
115. (2) High Altitude
A s distance from sea level increases , partial pressure of gases in
atmosphere decreases
But, volume remains constant eg: 21% for O2
Leads to a progressive reduction in ambient O2 Hypoxia
Compensatory mechanisms net effect is right shift of ODC
116. Increased alveolar ventilation
Increased Hb production
Increase in 2,3 DPG
Increase in diffusing capacity of lungs
Increase in vascularity of tissues
Increase cellullar use of 02
117. Congenital Abnormalities
Hemoglobinopathies: ODC shifted to right or left depending on affinity of
abnormal Hb to O2
Deficiency of red cell metabolism
Pyruvate kinase deficiency : shift to right
d/t elevated 2,3 DPG levels
118. Carbon Monoxide Poisoning
Hb has 200 times higher affinity for CO than O2 50% saturated at
0.4mmhg
Displaces O2 from Hb
Increases O2 affinity of those hemoglobin unbound to CO
Together it produces a shift to left in ODC and over all decrease in 02
delivery
119. A/c MI: right shift with an elevated P50
Hypophosphataemia as occurs in starvation, vomiting, malabsorption etc
causes increased Hb-O2 affinity and shift ODC to left
120. Bohr Effect
• By Christian Bohr in 1904
• The effect of CO2 on the OHDC is known as the
Bohr Effect
• High PCO2 levels and low pH decrease affinity of
hemoglobin for oxygen (a right-ward shift). • This
occurs at the tissues where a high level of PCO2 and
acidemia contribute to the unloading of oxygen.
121. Occurs at feto-maternal interface.
CO2 & other metabolic products from
the fetal blood diffuses into maternal
blood making maternal blood more
acidic & fetal blood more alkaline.
DOUBLE
BOHR
EFFECT
122. DOUBLE BOHR EFFECT
• Reciprocal changes in acid - base balance that occur in maternal & fetal blood in
transit through the placenta
FETAL BLOOD MATERNAL BLOOD
Loss of CO2 Gain of CO2
Rise in pH Fall in pH
Leftward shift of ODC Rightward shift of ODC
125. ODC helps us to relate PO2 and Hb saturation
A left shift gives a warning that tissue oxygen delivery may be
compromised even when there is not much drop in PO2
All inhalational agents including N2O causes shift to right
Intravenous agents have no demonstrable effect on ODC
126. Among other drugs : propranalol , steroids have been found to be
associated with shift to right and improved tissue oxygenation
Blood transfusion : whenever possible, ACD anticoagulated fresh
blood (<5-7 days old) should be used and avoid massive
transfusions.
Just as the heart has 2 blood supplies (one of the blood that needs to be oxygenated and pumped to the rest of the body as the cardiac output! And the other that supplies the cardiac muscle itself with nutrition), so does the lung. One consists of the blood from the right ventricle that needs to be oxygenated and send back to the left ventricle to be pumped to the rest of the body and the other is the blood that provides the nutrition to the lung conducting airways and tissue itself.