Bacterial Blotch Disease

Objective Analysis of Bacterial Blotch Disease Symptom Development on Agaricus bisporus Mushroom Tissue Plant Pathology Department June 22, 2007 by Aaron D. Brown

Presentation Outline: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Mushroom Industry: Overview ,[object Object],[object Object],[object Object],[object Object]

Bacterial Blotch Disease: Significance ,[object Object],[object Object],[object Object],[object Object],[object Object]

Bacterial Blotch Disease: Pathogen ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Bacterial Blotch Disease: Symptoms ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Research Objectives: Objective 1 : Determine minimum inhibitory concentration for several chemicals available for use on mushroom farms. Objective 2 : Develop objective method for the analysis of disease symptom severity on Agaricus bisporus mushroom tissue. Objective 3 : Determine influence of sodium hypochlorite and isolate 66R on disease symptom severity on A. bisporus tissue.

Pseudomonas tolaasii : Phenotype Switching ,[object Object],[object Object],[object Object],[object Object]

Phenotype Characteristics: Smooth (66S) Rough (66R) Opaque Mucoid Non-Fluorescent Pathogenic Tolaasin (+) Translucent Non-Mucoid Fluorescent Non-Pathogenic Tolaasin (-)

Phenotype Isolation: 66R and 66S

White Line Test: ,[object Object],[object Object],66R 66S 66W

Mushroom Tissue Block Test: 0 hr 24 hr SDW 66S 66R 66W ,[object Object],[object Object]

Objective 1 : Determine minimum inhibitory concentration for several chemicals available for use on mushroom farms. Methodology: ,[object Object],[object Object],[object Object],[object Object],[object Object]

Trade Names of Chemicals Tested: Bleach 5.25% sodium hypochlorite stock solution OxiDate  27% hydrogen dioxide stock solution MDF-500  3.2% ammonium chlorides 7.95% hydrogen peroxide stock solution Calcium chloride 96% calcium chloride anhydrous powder

Agar Well Diffusion Assay: Calcium chloride Hydrogen dioxide

Zone of Inhibition: Chemical Treatments

Minimum Inhibitory Concentrations: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],0.03% N/A 0.10% 5.0% Oxidate  CaCl 2 Bleach MDF-500 

Objective 2 : Develop objective method for the analysis of disease symptom severity on Agaricus bisporus tissue. Methodology: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Disease Symptoms: Color Variance P. reactans P. gingeri P. tolaasii ,[object Object],[object Object],[object Object]

Disease Symptom Severity: Isolates 0 hr 24 hr 66W 66R SDW 66S ,[object Object]

Analysis of Symptom Severity: Example 140.32 68.03 66W 66W SDW SDW 0 hr 5 hr 10 hr 24 hr

Symptoms Severity of Isolates: Results

Objective 3 : Determine influence of sodium hypochlorite and isolate 66R on disease symptom severity on A. bisporus tissue. Methodology: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Chemical and Bacterial Treatments: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Analysis of Chemical Treatment: Example 66W 66W SDW SDW 0 hr 1 hr 24 hr 168.58 95.31 0.1% 0.1% SDW SDW

Pre-Treatment: Post-Treatment: ,[object Object],[object Object],[object Object],[object Object]

Symptom Severity: 66R Pre-treatment 0 hr 24 hr ,[object Object],[object Object]

66R Pre-Treatment: Results ,[object Object]

Overview Research Results: ,[object Object],[object Object],[object Object],[object Object],[object Object]

Concluding Remarks: ,[object Object],[object Object],[object Object],[object Object]

Special Acknowledgements: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],The Pennsylvania State University

Are there any questions? Thank You for your attention!

Bacterial Blotch Disease

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