PROCESS OF INDUCED BREEDING OF INDIAN MAJOR CARP (IMC)

1. PINAKI BANERJEE
M.SC COASTAL AQUACULTURE
AND MARINE BIOTECHNOLOGY
3rd sem.
INDUCED BREEDING OF INDIAN
MAJOR CARP
INTERNAL GUIDANCE – DR.I.R.SIRISHA
EXTERNAL GUIDANCE – DR.MONJIT PAUL

2. *WHAT IS INDUCED BREEDING?
*WHY FISH DOES NOT BREED IN CAPTIVITY?
*WHY INDUCED BREEDING IS NECESSARY FOR FISH CULTURE?
*BROODERS SELECTION FOR INDUCED BREEDING.
*INDUCED BREEDING TECHNIQUE BY PITUITARY EXTRACT
STEPS ( *Removal of Gland , *Preservation of Gland,
*Preparation of Gland Extract , *Injection to the Brooders,
*Doses , *Spawning )
*FACTORS INFLUENCING THE SPAWNING OF FISH.
*SUBSTITUTES OF FISH PITUITARY GLAND. ( HCG , Ovaprim )
*RISK OF INDUCED BREEDING
*CONCLUSIONS

3. *Induced breeding is a technique by which
the economically important fish (which
generally do not breed in captive
condition) are breed through artificial
stimulation.

4. *Many cultural farm fishes like IMC do not breed
in captivity. The reason may be environmental
and consequently hormonal.
* Certain environmental parameters like
photoperiods, rain, temperature, current of
water influence the hormonal activity from
pituitary and gonads.

5. *
*It gives pure spawn of certain species of
fishes under cultivation.
*It can fulfil any quantity of demand in
any time.
* The technique is very simple and does
not need too much technical assistance

6. * The brooders must be healthy enough and
ripe.
* 2 – 4 years of age is generally selected
* 1 – 5 kg body weight is preferable
* Brooders should be disease free.

7. *Removal of Gland
*Preservation of Gland
*Preparation of Gland Extract
*Injection to the Brooders
*Doses
*Spawning

8. *Removal through foramen magnum – the
foramen magnum was first exposed by
removing vertebral parts of skull.
* Fat is removed first by means of forceps
and then cotton piece.
* A pair of forceps then inserted into
foramen magnum dorsally to the brain and
anterior part of the brain now detached
and remaining is carefully lifted out
through the foramen magnum.
* The gland is then located and removed.

10. *Glands can be
preserved in 100%
ethyl alcohol
*Acetone can be used
for preservation
*Glycerin is also used
as preservation
media

11. * Gland is dried in air by using blotting
paper
* Gland is taken in tissue homogenizer with
little amount of distilled water
*The pituitary extract is then centrifuged
[2000-2500 RPM(Revolutions per minute)
@15-30 mints.] and only the supernatant
solution is used for injection

12. * The pituitary extract is administered
into the body of breeders by means of
hypodermic syringe either Intra
muscular (the needle is inserted either
in the caudal peduncle or in the
shoulder) or Intra peritoneal (the
injections are given in the bases of
paired pelvic fins and bases of pectoral
fin) with 45 degree angle.

13. The needle is inserted in the
shoulder/Dorsal base (intra
muscular injection)
The needle is inserted in the caudal
peduncle (intra muscular injection)

14. The needle is inserted in the
base of pair of pelvic fins (intra
peritoneal)
The needle is inserted in the base of
pectoral fin (intra peritoneal )

15. *Usually the female is given two dose
*1st dose of female- 2-3mg/kg of body wt.
* 2nd dose of female - After an interval of
time about 12hrs a second dose of 5 – 8mg
are given per kg of body wt. of female.
*The male was given the first and last dose
of injection with 2ND dose of female @ 2-
3mg/kg of body wt.

16. *After injection to the brooders a set of brooders are
released into breeding hapa 1:2 (Female:Male) ratio.
* The hapa measures the range of 3m × 1.5m × 1m for
breeders weighing to 3 to 5kgs.
* Closed meshed mosquito netting is preferred for
that purpose, as its meshes will allow a good
circulation of water and will also not let the laid
eggs and milt escape through the meshes.
* The fertilized eggs are transparent, pearl like
*unfertilized eggs are opaque or whitish.

18. *Climate - 24°C to 31°C with cloudy days
and rainy periods. Light drizzling following
heavy rains is ideal. In absence of rain
artificial showers are used.
*Water – Flowing water is preferred.
*DO - >5mg/lit

19. *HCG (Human Chorionic Gonadotropin )
*Ovaprim

20.  successful spawning could be
achieved by injecting HCG alone @
630-660 IU and also with HCG 240 IU
+ 12 mg carp pituitary per kg body
weight.

21. *The rates of fertilization and hatching were
generally higher in Ovaprim treatment when
compared to pituitary
*The spawning response time was almost equal
in both Ovaprim and pituitary treatments.

22. * Based on the observations of the present
study, the dosage of Ovaprim required for
brood fish of various species is as follows:
* Catla 0.40 to 0.50 ml/kg
* Rohu 0.30 to 0.40 ml/kg
* Mrigal 0.25 to 0.30 ml/kg
* Silver carp 0.50 to 0.70 ml/kg
* Grass carp 0.50 to 0.70 ml/kg
* Big head carp 0.50 ml/kg
* Bata 0.50 mI/kg
* Fringe-lipped carp 0.50 ml/kg

23. *At the time of giving injection if
there done some mistake like high
dose or other injection giving
position mistake then fish may die.
*If water quality and factors which
influence the spawning of fish is not
proper rate then organisms may not
breed.

24. *Fish hatchery operators should be trained
on better broodfish management, hatchery
management and nursery management to
produce quality fish seed.
*Government or financial institutions should
sponsor setting up of field laboratories for
assessing and monitoring fish seed quality.
*Greater support (technical as well as
financial) from government agencies
needed for sustainable fish seed
production

26. *.