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Applications of plant tissue culture

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Applications of plant tissue culture

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Applications of plant tissue culture

  1. 1. Summary :Plant Tissue Culture Advancement in plant tissue culture brings a revolutionary stage in field of Agriculture /Plant biotechnology. Plant tissue culture serve as back bone in such scientific revolution by introducing genetically transformed (transgenic) products. Plant regeneration by such process is quiet easy and feasible rather than cloning in animals. To get success in culture of plant cells, tissues /organs we have to follow precise methods and proper sterilized use of materials and nutritional medium such as growth regulators for the specimen (explant) according to its requirement. Within favorable conditions the plant cells can multiply and even can generate whole plant. Regeneration of plant is important factor for quantitative and qualitative production of genetic desired characteristic. A (totipotent cell) a cell having ability to regenerate is used for mass production and this phenomenon is termed as Clonal propagation. Types of Tissue culture According to the type of explant selection and growth medium, different methods are generated and applicable to such technology which are as follows. Callus Culture If we use a culture where we generate Calluses, such type of culture is known as callus culture. Mass of undifferentiated cells is generate from cell/tissue mostly uses meristematic cells (Callus formation). Organogenesis is enhance by taking a specific portion from callus by providing precise requirements. Cell Suspension Culture. It includes liquid medium where we grow single specific cell or small group of cells. In medium cell growth is enhanced by continuous aeration and chemical exchange. In this method we can generate specific desired traits via a process known as Somatic cell embryogenesis. Protoplast Isolation and Culture Plant cell without cell wall is termed as protoplast which is isolate by enzymatic action. Protoplasts are used to form hybrids resulting genetically transformed embryos. Callus is produced in such protoplasts and induces organogenesis or embryogenesis. Pollen /Anther Culture. During development of flowers the anther or pollen is use in culture. As it is haploid so during culture chromosomal duplication is induced by chemicals e.g. colchicine. In such culture we can produce recessive traits Plant organ culture Plant organs can easily grow in artificial sterile medium from a cell r tissue of respective organ or totipotent cells.
  2. 2. Application of plant tissue culture Some of the application of plant tissue culture are as follows, ͙ Plant tissue culture play important role in biotechnology for the service of mankind. ͙ Cell suspension culture is main factory for production of secondary metabolites ͙ Horticulture uses and propagation of plants is applicable for mass production. ͙ Soma clonal variation bring different diverse products (species) which favors environmental requirements. ͙ Better, quality and more food production is now possible by this technology. ͙ Production of medicines, vaccine ͙ Biological hazards can controlled by using this technology via Phytoremediation. The End

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