The growth and development of plants is regulated by a number of chemical substances which together exert a complex interaction to meet the needs of the plant. Five groups of plant hormones are well established; they are the auxins, gibberellins, cytokinins, abscisic acid and its derivatives, and ethylene. These substances are of wide distribution and may, in fact, occur in all higher plants. They are specific in their action, are active in very low concentrations, and regulate cell enlargement, cell division, cell differentiation, organogenesis, senescence and dormancy. Their action is probably sequential. Other hormones concerned with flower formation and reproduction, but as yet uncharacterized, have also been envisaged. The essential role of these substances is illustrated by cell and tissue cultures; without the addition of suitable hormones no development or cell division occurs. The effects of these very active substances on the production of secondary metabolites, particularly with a view to producing plants containing an enhanced proportion of active constituent, are of interest to pharmacognosists. In such studies the manner in which the results are recorded is all-important, particularly as the treatment may also influence the size of the test plant compared with the controls. For commercial purposes yield per hectare is an obvious criterion, whereas for biosynthetic studies yield per plant or per cent fresh weight may be of more significance. For final drug evaluation per cent dry weight is the most likely requirement.