Here is a presentation on different types of centrifuges that we also use.
also i have mentioned about different types of rotors.
william and walker gives a very nice information about centrifugation.
also there are many videos available on youtube. a pratical knowledge of this would work better.
Happy Studying :)
4. Small bench top centrifuges
• Maximum speed 4000 to 6000 rpm
• RCF is 3000 g force
• Can operate at ambient temperature
• Rotors used
5. Low speed centrifugation
• Refrigerated centrifuge
• Maximum speed of 6000 rev.per minute
• RCF is 6500g force
• Large carrying capacity
• Interchangeable rotors
• Accurate balance needs to be done
• For RBCs, nuclei and chloroplasts
6.
7. High speed centrifuge
• Very high speed
• They can go upto 25,000 rev perminute
• RCF is 60,000 g force
• Interchangeable rotors
• Used for pelleting micro organisms, cellular
debris and so on
8.
9. Ultracentrifuges
• Very high speed of 70,000 rpm and more
• Rotors are run in high vacuum
• Lot of sophistication is required
• Rotors do not have to be aerodynamically
designed
• RCF of 500,000 g force
• Can be used for membrane fractionation and
viruses and so on
10. Ultracentrifuges can be of 2 types
• Preparative ultracentrifuge
• Analytical ultracentrifuge
11. Preparative ultracentrifugation
• Maximum speed of 80,000 rpm
• RCF is 600,000 g force
• Sealed evacuated rotor chamber
• Temperature monitoring system
• Electronic circuits that detect rotor imbalance
• Flexible drive shaft
• Protected in armor shield
• Table top centrifuges present
• Used for sub cellular organelles, viruses etc.
12. Analytical ultracentrifuge
• They have a system for detection
• i.e. they have optical systems
• They have 3 types of optical systems
a) Absorbance optical system
b) Alternative schlieron system
c) Rayleigh optical system
16. References
• Principles and Techniques of Biochemistry and
Molecular Biology by wilson and walker
• http://www.thermoscientific.com
• http://www.phys.sinica.edu
