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Laboratory safety
Tapeshwar Yadav
(Lecturer)
BMLT, DNHE,
M.Sc. Medical Biochemistry
Introduction
Laboratory safety rules are a major aspect of every
clinical lab.
Each student in clinical laboratory must follow
specific safety rules and procedures.
Why is Lab Safety Important?
Lab safety rules and
symbols are needed so
that students do not injure
themselves or their
classmates.
Lab Safety Rules
Lab Safety Rules
1-Wear protective clothing .
Protective clothes
o Gloves are essential.
o Lab coats are required.
o Safety glasses
( goggles) may be
required to avoid
splashes.
2-Laboratory personnel should not wear sandals
Lab Safety Rules
Lab Safety Rules
Do NOT Wear:
• Jewelry
• Loose or Baggy clothing
3-Avoid touching objects (e.g., pencils, cell phones, door
handles) while wearing gloves.
Lab Safety Rules
4-Pencils, labels, or any other materials should never be
placed in your mouth.
5-Caution must be taken when using gas burners. Be
sure gas burners are turned off when finished.
6-Long hair must be tied back or covered to minimize fire
hazard or contamination of experiments.
Lab Safety Rules
Lab Safety Rules
7- Do not eat food or drink water in the lab.
do not use lab glassware as food or water
containers.
Lab Safety Rules
8- Protect your hands safety:
- wash hands after every lab.
- Handle glassware, sharp tools and heated containers carefully.
Lab Safety Rules
9- electrical safety:
- unplug electrical equipment after use.
- keep all electrical cords and wires away from water .
Lab Safety Rules
10- Chemical safety:
-never touch, taste or smell a chemical unless instructed to do so.
- never mix chemicals unless instructed to do so.
-keep lids on chemical containers when not in use.
Lab Safety Rules
11-Do not take any cultures out of the lab
for any reason.
All cultures should be handled as
potentially pathogenic.
Liquid cultures must always be kept in a
test tube rack.
Lab Safety Rules
12- Do not engage in practical jokes or horseplay in the
lab.
Lab Safety Rules
13-Keep nonessential books and clothing far away from
your work area.
Lab Safety Rules
14-Wipe the bench tops down with disinfectant both before you
begin your work and after you have completed your work.
Lab Safety Rules
15- Dispose of waste products according to instructions.
16-Report all accidents, no matter how minor, to
your supervisor!!
Lab Safety Rules
Lab Safety Equipment
Lab Safety Equipment
Safety shower
Eye wash
Lab Safety Equipment
Fire Blanket
Fire Extinguisher
Lab Safety Symbols
Lab Safety Symbols
• They alert about the possible dangers in the lab.
Biological Safety Levels
Biological Safety Levels
1. BSL1
2. BSL2
3. BSL3
4. BSL4
BSL-1
– Not known to cause disease in healthy adult
humans
• Safety equipment
– Minimal requirements
• Facilities
– Open bench top
BSL-2
– Associated with mild to moderate disease
in humans
• Safety equipment
– Biological Safety Cabinet and personal
protective equipment as needed.
• Facilities
– BSL-1 plus the availability of a
mechanism for decontamination.
BSL-3
– Associated with serious or potentially
lethal disease in humans
• Safety equipment
– Biological Safety Cabinet and
personal protective equipment
required.
• Facilities
– BSL-2 with self-closing double door
access and single-pass negative
directional airflow.
BSL-4
– Associated with high risk of life-
threatening disease in humans
and/or animals
• Safety equipment
– Biological Safety Cabinet
– Full-body air-supplied, positive
pressure personnel suit
• Facilities
– BSL-3 plus dedicated air and
exhaust, decontamination
procedures for exit, separate
building, etc.

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