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Thermo Fisher Scientific • Street Address • City, ST ZIP Code • thermofisher.com
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NSCLC Tumor NSCLC Tumor NSCLC Tumor NSCLC Tumor NSCLC Tumor NSCLC Tumor Uterus:
Endometrium
Tumor
NSCLC Tumor NSCLC Tumor
Mutations/Mb
TMB Measurements of FFPE Tumor Tissue; Untreated
vs UDG Treated
Mut/Mb; Untreated Rep 1 Mut/Mb; Untreated Rep 2 Mut/Mb; UNG Treated Rep 1
Mut/Mb; UNG Treated Rep 2 Mut/Mb; UNG Treated Rep 3 Mut/Mb; UNG Treated Rep 4
Figure 4. FFPE DNA Repair Reproducibility
Figure 5. UDG Treatment on FFPE Normal Tissue Repairs Deamination
ABSTRACT
Tumor mutational burden (TMB) is a positive predictive factor for response to
immune-checkpoint inhibitors in certain types of cancer. The Oncomine™ Tumor
Mutation Load Assay, a targeted next generation sequencing (NGS) assay,
measures TMB (from 1.2Mb of coding region) and detects mutations in 409 cancer
genes. The TMB values obtained using targeted sequencing are highly correlated
with TMB measured by whole exome sequencing. FFPE preservation methods can
lead to significant cytosine deamination of the isolated DNA, resulting in decreased
sequencing quality. In these samples, uracils are propagated as thymines and result
in false C>T substitutions. Analysis of the Oncomine™ TML Assay using Torrent
Suite and Ion Reporter ™ software uniquely estimates the degree of deamination in
fixed tissues by measuring C:G>T:A variants. This deamination score is used to
assess quality of DNA extracted from FFPE tumor tissue. To minimize the influence
that excess deamination has on TMB results, we have incorporated a repair
treatment to eliminate damaged targets and improve usable TMB values of DNA
from damaged FFPE tumor tissue using Uracil-DNA glycosylase (UDG). The
Oncomine™ TML Assay for TMB on the Ion Gene Studio™ S5 systems in
conjunction with a deamination score is informative and potentially predictive for the
use of checkpoint inhibitors in multiple cancer types.
INTRODUCTION
Multiple FFPE repair kits are commercially available to treat genomic DNA extracted
from FFPE tissues. Treatment typically involves enzymatic incubation and
purification outside of the intended workflow, which can be time consuming and may
result in loss of precious material. The proposed repair with UDG is integrated into
the Oncomine™ TML Assay workflow, consisting of a short incubation with no
purification step. This method allows for minimal intrusion of the original
Oncomine™ TML Assay workflow, with no loss in sample amount.
MATERIALS AND METHODS
Cytosine deamination in FFPE DNA can propagate false C>T mutations, which can
inflate tumor mutational burden results. Experiments were performed to compare
TMB results of UDG treated FFPE DNA to non-treated FFPE DNA. Selection of
FFPE DNA with range of TMB results and deamination scores (assigned by Ion
Reporter ™ TML workflow) were chosen to test the efficacy of UDG treatment. The
Ion Reporter ™ deamination score is reported as the “Estimated SNP proportion
consistent with deamination (mainly FFPE)”.
Libraries from FFPE DNAs, using 20ng input total per sample, were generated with
the Oncomine™ Tumor Mutation Load Assay (A37909) under the standard protocol
(MAN0071042). In parallel, libraries from the same FFPE DNAs were generated
under the standard protocol after UDG treatment. The UDG treatment occurs prior
to the target amplification with the TML assay itself. For each FFPE DNA sample,
the following components are added to a single well of a 96-well PCR plate:
Plate is sealed and incubated in Applied Biosystems Verti ™ 96-well thermal cycler:
37ºC for 2 minutes, 50C for 10 minutes, and 4C hold for no more than 60min.
UDG treated FFPE DNA is now ready to use with the standard TML Assay protocol,
beginning with target amplification. Completed libraries were pooled, templated with
Ion 540 ™ Kit-Chef, and sequenced on Ion Gene Studio ™ S5 Plus System with Ion
540 ™ chips.
Data analysis was completed with Torrent Suite ™ 5.10 and Ion Reporter ™ 5.10.
TMB values (Mutations/Mb) was determined using Oncomine™ Tumor Mutation
Load - w2.0 - DNA - Single sample workflow.
CONCLUSIONS
Incorporation of a repair of DNA extracted from FFPE tissues as part of the Oncomine™
TML Assay workflow show consistent and effective reduction of C>T artifacts reflective of
deamination without affecting true variants. Without UDG treatment, deamination damage
propagates false C>T mutations, thus inflates TMB measurements. UDG treatment
corrects for deamination and provides results of TMB measurements in a biological relevant
range. UDG treatment would be an effective inclusion to the Oncomine™ Tumor Mutation
Load Assay workflow.
REFERENCES
1. Henn B et al., Nature Reviews Genetics 16(6) 333-343 2015 Estimating The Mutation
Load in Human Genomes
ACKNOWLEDGEMENTS
FFPE tissue was purchased from the following vendors: Biochain Institute Inc, BioIVT, and
Discovery Life Sciences.
We acknowledge and thank James Commins and Erin Lagier for sample preparation of
FFPE tissues.
RESULTS
Table 1. Comparison of Tumor Mutational Burden Measurement (Mutations/Mb) between
non-treated and UDG treated FFPE DNA
Initial testing of UDG treatment of FFPE DNA showed reduction of mutations/Mb correspond with decrease in
deamination score. UDG treatment did not affect TMB score of samples with low deamination scores and minimal
damage. Initial UDG treatment experiment shows significant reduction in deamination scores, in turn reducing
Mutations/Mb to a biologically relevant level. However, some samples with very high deamination scores could not
be rescued as FFPE damage is too great to overcome.
Warren Tom, Ruchi Chaudhary, Vinay Mittal, Dinesh Cyanam, Iris Casuga, Elaine Wong-Ho, Rob Bennett, Fiona Hyland, Seth Sadis and Janice Au-Young. Thermo Fisher Scientific, South San
Francisco, CA 94080
IMPROVEMENT OF TUMOR MUTATION BURDEN MEASUREMENT BY REMOVAL OF DEAMINATED
BASES IN FFPE DNA
Component Volume (µL)
20ng FFPE DNA 7.3
UDG, heat-labile 1
Low TE to 8.3
Sample Mutations/Mb, Untreated Deamination Score, Untreated
Mutation/Mb, UDG
Treated Deamination Score, UDG Treated
810145CN 3.38 0 3.4 0
F00092860b 5.87 14 0.84 0
F00092861b 33.84 8 31.45 0
810145CT 60.91 23 60.86 25
810136T 166.52 272 59.75 68
B810137T 305.99 618 28.18 28
B810136N 1661.09 2829 328.24 290
B810135T 5794.99 9018 800.53 610
810145CN – FFPE Colon Normal Tissue.
Overlap analysis of variants called, show
agreement between untreated and UDG treated
DNA. UDG treatment did not affect the calling of
variants.
B810137T – FFPE Lung Tumor Tissue
Overlap analysis shows true variants from the
sample, with untreated(blue) sample showing
large number of variants (627) not consistent
between the two treatments. The 627
variants are likely deaminated bases that
cause FPs contributing to the higher TMB.
The UDG treated sample show a lower
number of variants outside of the overlap,
which correlates to the lower TMB.
TMB measurement comparisons show the effectiveness of UDG across a variety of samples and range TMB
measurements. Samples with TMB measurements above a score of 50 would require additional review to
determine sample quality. With UDG treatment, a majority of the same samples result with Mut/Mb in a relevant
range. Samples with minimal reduction show true Mut/Mb measurement with low or no deamination score.
FFPE Normal Adjacent to Tumor (NAT) Tissues with varying degrees of deamination, resulted in low TMB
scores post UDG treatment. FPs that occur in untreated samples have been repaired and FFPE Normal
Tissue TMB measurements are in-line with normal cell line TMB measurements. Replicate libraries from the
same source show consistent performance of the UDG treatment to reduce deamination of FFPE damage
Normal Tissues.
Duplicate libraries of UDG treated FFPE samples,
generated across two users with different lots of library
and sequencing reagents and different instrumentation,
show high reproducibility (total n=4 per FFPE sample).
Aggregate correlation across users show high correlation
of Mut/Mb results. Study shows consistent performance of
UDG treatment to repair deamination.
Figure 7. Substitution Type and Context of Somatic Mutations – Sample with low
deamination
UDG treated FFPE Tumor Tissue show reduction of Mut/Mb measurement. For some samples, high TMB is
only slightly reduced when associated with low deamination score to begin with (A). Tumor samples with TMB
once considered too high to be relevant, has TMB reduced to a biological relevant level with UDG treatment (B).
Figure 1. Variant Overlap Analysis
Example: FFPE Normal Sample
Figure 2. Variant Overlap Analysis
Example: FFPE Tumor with high
deamination.
Figure 3. Tumor Mutation Load Measurements (Mutations/Mb) Comparison: Untreated vs
UDG Treatment
Figure 6. UDG Treatment on FFPE Tumor Tissue Repairs Deamination
Figure 8. Substitution Type and Context of Somatic Mutations – Sample with high
deamination
A B
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NSCLCNAT
F52023.4Hb
NSCLCNAT
F51986.5Db
NSCLCTumor
F42644.4Jd
NSCLCTumor
F42634.5Ga
NSCLCNormal
F41486.7Md
NSCLCNAT
F41512.2Ib
NSCLCNAT
F41501.2Ib
NSCLCNAT
F41516.Fb
EndometrialCancerNAT
F28291.A8a
NSCLCNormal
F33413.Bb
NSCLCTumor
F41511.2Bd
NSCLCTumor
F42756.3Da
NSCLCTumor
F42738.3Ic
EndometrialCancerTumor
F28290.A9f
Uterus:EndometriumNormal
F28536.A14c
Uterus:EndometriumTumor
F28537.A7c
Uterus:MyometriumNormal
F28216.A7b
NSCLCTumor
F41515.Da
NSCLCTumor
F33414.Lb
NSCLCTumor
F41500.2Ab
Uterus:EndometriumTumor
F28215.A6b
NSCLCTumor
F41484.7C2d
NSCLCNAT
F12567.A2bb
NSCLCTumor
F12566.A4c
NSCLCNAT
F53217.3Hb
NSCLCNAT
F53206.3Bb
DeaminationScore(cutoffat500)
Mutations/Mb(cutoffat100)
Mutations/Mb and Deamination Score Comparison;
FFPE Tumor and FFPE NAT
Mut/Mb Untreated Mut/Mb UDG treated Deam Score; Untreated Deam Score; UDG treated
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NSCLC NAT NSCLC NAT NSCLC NAT NSCLC NAT NSCLC NAT Endometrial
Cancer NAT
NSCLC NAT
Mutations/Mb
TMB Measurements of FFPE Normal Tissue;
Untreated vs UDG Treated
Mut/Mb; Untreated Rep 1 Mut/Mb; Untreated Rep 2 Mut/Mb; UNG Treated Rep 1
Mut/Mb; UNG Treated Rep 2 Mut/Mb; UNG Treated Rep 3 Mut/Mb; UNG Treated Rep 4
A – Untreated Mut/Mb = 49.8;
Deamination Score = 15
A – UDG treated Mut/Mb = 43.42;
Deamination Score = 5
Substitution profile show minimal reduction of C>T events after UDG treatment, reflective of minimal decrease
in Mut/Mb measurement. Other substitution events remained unchanged.
B – Untreated Mut/Mb = 177.52;
Deamination Score = 176
B – UDG treated Mut/Mb = 11.79;
Deamination Score = 21
Substitution profile shows effective reduction of C>T events after UDG treatment, reflective of decrease in
Mut/Mb measurement. Other substitution events remained unchanged.
F00092860b – FFPE Colon Normal Tissue.
Overlap analysis shows untreated sample with
higher discordant variants, corresponding to
higher deamination score. UDG treated sample
generation a zero deamination score, reflective of
absence of deamination.
A
B
1701
R² = 0.9326
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60
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100
0 20 40 60 80
Mutations/MbUser1Replicates
Mutations/Mb User 2 Replicates
Correlation of Mutations/Mb Of UDG Treated
FFPE Sample Replicates
User
1_Rep_1
User
1_Rep_2
User
2_Rep_1
User
2_Rep_2
User
1_Rep_1
1
User
1_Rep_2
0.97 1
User
2_Rep_1
0.98 0.91 1
User
2_Rep_2
0.99 0.98 0.97 1
Table 2. Replicate Correlation
Comparison of individual replicates and users –
Pearson correlation is high across users and
replicates. Workflow for FFPE DNA repair show
robust effect of UDG treatment.
Thermo Fisher Scientific – 180 Oyster Point Blvd – South San Francisco, CA 94080 – thermofisher.com
For Research Use Only. Not for use in diagnostic procedures.

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Improvement of TMB Measurement by removal of Deaminated Bases in FFPE DNA

  • 1. Thermo Fisher Scientific • Street Address • City, ST ZIP Code • thermofisher.com 0 10 20 30 40 50 60 70 80 90 100 NSCLC Tumor NSCLC Tumor NSCLC Tumor NSCLC Tumor NSCLC Tumor NSCLC Tumor Uterus: Endometrium Tumor NSCLC Tumor NSCLC Tumor Mutations/Mb TMB Measurements of FFPE Tumor Tissue; Untreated vs UDG Treated Mut/Mb; Untreated Rep 1 Mut/Mb; Untreated Rep 2 Mut/Mb; UNG Treated Rep 1 Mut/Mb; UNG Treated Rep 2 Mut/Mb; UNG Treated Rep 3 Mut/Mb; UNG Treated Rep 4 Figure 4. FFPE DNA Repair Reproducibility Figure 5. UDG Treatment on FFPE Normal Tissue Repairs Deamination ABSTRACT Tumor mutational burden (TMB) is a positive predictive factor for response to immune-checkpoint inhibitors in certain types of cancer. The Oncomine™ Tumor Mutation Load Assay, a targeted next generation sequencing (NGS) assay, measures TMB (from 1.2Mb of coding region) and detects mutations in 409 cancer genes. The TMB values obtained using targeted sequencing are highly correlated with TMB measured by whole exome sequencing. FFPE preservation methods can lead to significant cytosine deamination of the isolated DNA, resulting in decreased sequencing quality. In these samples, uracils are propagated as thymines and result in false C>T substitutions. Analysis of the Oncomine™ TML Assay using Torrent Suite and Ion Reporter ™ software uniquely estimates the degree of deamination in fixed tissues by measuring C:G>T:A variants. This deamination score is used to assess quality of DNA extracted from FFPE tumor tissue. To minimize the influence that excess deamination has on TMB results, we have incorporated a repair treatment to eliminate damaged targets and improve usable TMB values of DNA from damaged FFPE tumor tissue using Uracil-DNA glycosylase (UDG). The Oncomine™ TML Assay for TMB on the Ion Gene Studio™ S5 systems in conjunction with a deamination score is informative and potentially predictive for the use of checkpoint inhibitors in multiple cancer types. INTRODUCTION Multiple FFPE repair kits are commercially available to treat genomic DNA extracted from FFPE tissues. Treatment typically involves enzymatic incubation and purification outside of the intended workflow, which can be time consuming and may result in loss of precious material. The proposed repair with UDG is integrated into the Oncomine™ TML Assay workflow, consisting of a short incubation with no purification step. This method allows for minimal intrusion of the original Oncomine™ TML Assay workflow, with no loss in sample amount. MATERIALS AND METHODS Cytosine deamination in FFPE DNA can propagate false C>T mutations, which can inflate tumor mutational burden results. Experiments were performed to compare TMB results of UDG treated FFPE DNA to non-treated FFPE DNA. Selection of FFPE DNA with range of TMB results and deamination scores (assigned by Ion Reporter ™ TML workflow) were chosen to test the efficacy of UDG treatment. The Ion Reporter ™ deamination score is reported as the “Estimated SNP proportion consistent with deamination (mainly FFPE)”. Libraries from FFPE DNAs, using 20ng input total per sample, were generated with the Oncomine™ Tumor Mutation Load Assay (A37909) under the standard protocol (MAN0071042). In parallel, libraries from the same FFPE DNAs were generated under the standard protocol after UDG treatment. The UDG treatment occurs prior to the target amplification with the TML assay itself. For each FFPE DNA sample, the following components are added to a single well of a 96-well PCR plate: Plate is sealed and incubated in Applied Biosystems Verti ™ 96-well thermal cycler: 37ºC for 2 minutes, 50C for 10 minutes, and 4C hold for no more than 60min. UDG treated FFPE DNA is now ready to use with the standard TML Assay protocol, beginning with target amplification. Completed libraries were pooled, templated with Ion 540 ™ Kit-Chef, and sequenced on Ion Gene Studio ™ S5 Plus System with Ion 540 ™ chips. Data analysis was completed with Torrent Suite ™ 5.10 and Ion Reporter ™ 5.10. TMB values (Mutations/Mb) was determined using Oncomine™ Tumor Mutation Load - w2.0 - DNA - Single sample workflow. CONCLUSIONS Incorporation of a repair of DNA extracted from FFPE tissues as part of the Oncomine™ TML Assay workflow show consistent and effective reduction of C>T artifacts reflective of deamination without affecting true variants. Without UDG treatment, deamination damage propagates false C>T mutations, thus inflates TMB measurements. UDG treatment corrects for deamination and provides results of TMB measurements in a biological relevant range. UDG treatment would be an effective inclusion to the Oncomine™ Tumor Mutation Load Assay workflow. REFERENCES 1. Henn B et al., Nature Reviews Genetics 16(6) 333-343 2015 Estimating The Mutation Load in Human Genomes ACKNOWLEDGEMENTS FFPE tissue was purchased from the following vendors: Biochain Institute Inc, BioIVT, and Discovery Life Sciences. We acknowledge and thank James Commins and Erin Lagier for sample preparation of FFPE tissues. RESULTS Table 1. Comparison of Tumor Mutational Burden Measurement (Mutations/Mb) between non-treated and UDG treated FFPE DNA Initial testing of UDG treatment of FFPE DNA showed reduction of mutations/Mb correspond with decrease in deamination score. UDG treatment did not affect TMB score of samples with low deamination scores and minimal damage. Initial UDG treatment experiment shows significant reduction in deamination scores, in turn reducing Mutations/Mb to a biologically relevant level. However, some samples with very high deamination scores could not be rescued as FFPE damage is too great to overcome. Warren Tom, Ruchi Chaudhary, Vinay Mittal, Dinesh Cyanam, Iris Casuga, Elaine Wong-Ho, Rob Bennett, Fiona Hyland, Seth Sadis and Janice Au-Young. Thermo Fisher Scientific, South San Francisco, CA 94080 IMPROVEMENT OF TUMOR MUTATION BURDEN MEASUREMENT BY REMOVAL OF DEAMINATED BASES IN FFPE DNA Component Volume (µL) 20ng FFPE DNA 7.3 UDG, heat-labile 1 Low TE to 8.3 Sample Mutations/Mb, Untreated Deamination Score, Untreated Mutation/Mb, UDG Treated Deamination Score, UDG Treated 810145CN 3.38 0 3.4 0 F00092860b 5.87 14 0.84 0 F00092861b 33.84 8 31.45 0 810145CT 60.91 23 60.86 25 810136T 166.52 272 59.75 68 B810137T 305.99 618 28.18 28 B810136N 1661.09 2829 328.24 290 B810135T 5794.99 9018 800.53 610 810145CN – FFPE Colon Normal Tissue. Overlap analysis of variants called, show agreement between untreated and UDG treated DNA. UDG treatment did not affect the calling of variants. B810137T – FFPE Lung Tumor Tissue Overlap analysis shows true variants from the sample, with untreated(blue) sample showing large number of variants (627) not consistent between the two treatments. The 627 variants are likely deaminated bases that cause FPs contributing to the higher TMB. The UDG treated sample show a lower number of variants outside of the overlap, which correlates to the lower TMB. TMB measurement comparisons show the effectiveness of UDG across a variety of samples and range TMB measurements. Samples with TMB measurements above a score of 50 would require additional review to determine sample quality. With UDG treatment, a majority of the same samples result with Mut/Mb in a relevant range. Samples with minimal reduction show true Mut/Mb measurement with low or no deamination score. FFPE Normal Adjacent to Tumor (NAT) Tissues with varying degrees of deamination, resulted in low TMB scores post UDG treatment. FPs that occur in untreated samples have been repaired and FFPE Normal Tissue TMB measurements are in-line with normal cell line TMB measurements. Replicate libraries from the same source show consistent performance of the UDG treatment to reduce deamination of FFPE damage Normal Tissues. Duplicate libraries of UDG treated FFPE samples, generated across two users with different lots of library and sequencing reagents and different instrumentation, show high reproducibility (total n=4 per FFPE sample). Aggregate correlation across users show high correlation of Mut/Mb results. Study shows consistent performance of UDG treatment to repair deamination. Figure 7. Substitution Type and Context of Somatic Mutations – Sample with low deamination UDG treated FFPE Tumor Tissue show reduction of Mut/Mb measurement. For some samples, high TMB is only slightly reduced when associated with low deamination score to begin with (A). Tumor samples with TMB once considered too high to be relevant, has TMB reduced to a biological relevant level with UDG treatment (B). Figure 1. Variant Overlap Analysis Example: FFPE Normal Sample Figure 2. Variant Overlap Analysis Example: FFPE Tumor with high deamination. Figure 3. Tumor Mutation Load Measurements (Mutations/Mb) Comparison: Untreated vs UDG Treatment Figure 6. UDG Treatment on FFPE Tumor Tissue Repairs Deamination Figure 8. Substitution Type and Context of Somatic Mutations – Sample with high deamination A B 0 50 100 150 200 250 300 350 400 450 500 0 10 20 30 40 50 60 70 80 90 100 NSCLCNAT F52023.4Hb NSCLCNAT F51986.5Db NSCLCTumor F42644.4Jd NSCLCTumor F42634.5Ga NSCLCNormal F41486.7Md NSCLCNAT F41512.2Ib NSCLCNAT F41501.2Ib NSCLCNAT F41516.Fb EndometrialCancerNAT F28291.A8a NSCLCNormal F33413.Bb NSCLCTumor F41511.2Bd NSCLCTumor F42756.3Da NSCLCTumor F42738.3Ic EndometrialCancerTumor F28290.A9f Uterus:EndometriumNormal F28536.A14c Uterus:EndometriumTumor F28537.A7c Uterus:MyometriumNormal F28216.A7b NSCLCTumor F41515.Da NSCLCTumor F33414.Lb NSCLCTumor F41500.2Ab Uterus:EndometriumTumor F28215.A6b NSCLCTumor F41484.7C2d NSCLCNAT F12567.A2bb NSCLCTumor F12566.A4c NSCLCNAT F53217.3Hb NSCLCNAT F53206.3Bb DeaminationScore(cutoffat500) Mutations/Mb(cutoffat100) Mutations/Mb and Deamination Score Comparison; FFPE Tumor and FFPE NAT Mut/Mb Untreated Mut/Mb UDG treated Deam Score; Untreated Deam Score; UDG treated 0 10 20 30 40 50 60 70 80 90 NSCLC NAT NSCLC NAT NSCLC NAT NSCLC NAT NSCLC NAT Endometrial Cancer NAT NSCLC NAT Mutations/Mb TMB Measurements of FFPE Normal Tissue; Untreated vs UDG Treated Mut/Mb; Untreated Rep 1 Mut/Mb; Untreated Rep 2 Mut/Mb; UNG Treated Rep 1 Mut/Mb; UNG Treated Rep 2 Mut/Mb; UNG Treated Rep 3 Mut/Mb; UNG Treated Rep 4 A – Untreated Mut/Mb = 49.8; Deamination Score = 15 A – UDG treated Mut/Mb = 43.42; Deamination Score = 5 Substitution profile show minimal reduction of C>T events after UDG treatment, reflective of minimal decrease in Mut/Mb measurement. Other substitution events remained unchanged. B – Untreated Mut/Mb = 177.52; Deamination Score = 176 B – UDG treated Mut/Mb = 11.79; Deamination Score = 21 Substitution profile shows effective reduction of C>T events after UDG treatment, reflective of decrease in Mut/Mb measurement. Other substitution events remained unchanged. F00092860b – FFPE Colon Normal Tissue. Overlap analysis shows untreated sample with higher discordant variants, corresponding to higher deamination score. UDG treated sample generation a zero deamination score, reflective of absence of deamination. A B 1701 R² = 0.9326 0 20 40 60 80 100 0 20 40 60 80 Mutations/MbUser1Replicates Mutations/Mb User 2 Replicates Correlation of Mutations/Mb Of UDG Treated FFPE Sample Replicates User 1_Rep_1 User 1_Rep_2 User 2_Rep_1 User 2_Rep_2 User 1_Rep_1 1 User 1_Rep_2 0.97 1 User 2_Rep_1 0.98 0.91 1 User 2_Rep_2 0.99 0.98 0.97 1 Table 2. Replicate Correlation Comparison of individual replicates and users – Pearson correlation is high across users and replicates. Workflow for FFPE DNA repair show robust effect of UDG treatment. Thermo Fisher Scientific – 180 Oyster Point Blvd – South San Francisco, CA 94080 – thermofisher.com For Research Use Only. Not for use in diagnostic procedures.