This document provides guidelines for laboratory animal care according to the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA). It outlines standards for veterinary care, animal procurement, quarantine, housing and management, transportation, anesthesia, and euthanasia. The goals are to promote humane animal care and avoid unnecessary pain during experiments. Key aspects covered include adequate veterinary services, health screening of procured animals, quarantine periods, physical separation of species, routine health monitoring, hygienic facilities and equipment, controlled environment conditions, and pain management procedures. The guidelines aim to ensure proper care, housing and experimental procedures for laboratory animals.

1. CPCSEA GUIDELINE
FOR LABORATORY
ANIMAL
•
Submitted to
Dr.Nagraj sir
Hod of dept
Pharmaceutical Analysis
Presented by
Abhishek Mondal
1st Mpharm
Dept of
pharmacology

2. Contents:
• INTRODUCTION
• OBJECTIVES
• VETERINARY CARE
• ANIMAL PROCUREMENT
• QURANTINE , STABILIZATION , SEPARATION
• TRANSPORT OF ANIMAL
• LABORATORY ANIMAL HUSBANDARY & MANGE MENT
• ANAESTHESIA AND EUTHANASIA

3. WHAT IS CPCSEA
Committee for the purpose of control and supervision of
experiments on animals.
CORE MEMBER:
Hon. Smt. Maneka Gandhi – drafted under chairperson, CPCSEA
Mr. A.K.Joshi – Member Secretary, CPCSEA
SUBCOMMITTEE MEMBERS:
Dr. Manju Sharma – secretary, department of biotechnology
Members
Dr. vasanth muthuswamy- Sr. DDG, Indian council of medical research
Dr. Lal Krishna – ADG,(AH) ICAR, New Delhi
Dr. S.S. Murugan – SGS India private limited, Chennai

4. FUNCTION OF CPCSEA
 Registration of establishments conducting animal
experimentation or breeding of animals for this purpose.
 Selection and appointment of nominees in the Institutional
Animal Ethics Committees of registered establishments.
 Approval of Animal House Facilities on the basis of reports of
inspections conducted by CPCSEA.
 Permission for conducting experiments involving use of
animals.
 Recommendation for import of animals for use in
experiments.
 Action against establishments in case of violation of any legal
norm/stipulation.

5. WHY ANIMALS ARE USED IN RESEARCH

6. Reasons
 The principle of anatomy and physiology are true for human
and , especially mammals .
 Certain strains or breeds of animals get the same disease or
conditions as humans.
 With animals we can control their environment(temperature ,
humidity, etc) and shield them from disease or condition not
related to the research ( control their health).
 We can use scientifically – valid no of animals.

7. Objectives
• The goal of these guidelines is to promote the human
care of animal used in biomedical and behavioural
research and testing.
• To avoid unnecessary pain before ,during and after
experiment .
• To provide guideline for
-housing ,care ,breeding and maintenance
-source of experimentals animals
-acceptables experimentals procedures for anesthesia
and euthanasia

8. Veterinary care
o Adequate veterinary care must be provided and is the
responsibility of a veterinarian or a person who has
training or experience 'in laboratory animal sciences and
medicine.
o Observed regularly for sign of illness, injury, or
abnormal behavior
o Contagious disease – isolated from healthy animal.

9. Animal procurement
• All animals must be acquired lawfully as per the CPCSEA
guidelines.
• A health surveillance program for screening incoming animals
should be carried out to assess animal quality.
• Methods of transportation should also be taken into account.
• Each consignment of animals should be inspected for
compliance with procurement specifications, and the animals
should be quarantined and stabilized according to procedures
appropriate for the species and circumstances.

10. QURANTINE ,
STABILIZATION
SEPARATION

11. QUARANTINE
• Separation of newly received animals from those already
in the facility until the health and possibly the microbial
status of newly received animal have been determine.
• A minimum duration of quarantine for small animal-1
week and for larger animal-6 week.
• An effective quarantine minimizes the chance for
introduction of pathogens into an established colony.

12. STABILIZATION AND SAPARATION
STABILIZATION-
 Physiologic, psychological and nutritional stabilization
should be given before their use.
 Duration of stabilization will depend on type and
duration of animal transportation , and species of animal.
SEPARATION-
 Physical separation of animal by species is
recommended to prevent interspecies disease
transmission and to eliminate anxiety and possible
physiological and behavioural changes due to
interspecies conflict.
 It shall be acceptable to house different species in the
same room ,e.g. two species have a similar pathogen
status and are behaviourally compatible.

13. SURVELLANCE, DIAGNOSIS, TRETMENT AND CONTROL
DISEASE
• All animal should be observed for signs of illness, injury,
or abnormal behaviour by animal house staff.
• Animals that show signs of a contagious disease should
be isolated from healthy animals in the colony.

14. PERSONAL HYGIENE
• Animal care staff maintain a high standard of personal
cleanliness.
• Clothing suitable for use in the animal facility .
• It acceptable to use disposable gear such as gloves,
masks, head covers, coats, coveralls and shoe covers.
• Person should change clothing as often as is necessary
to maintain personal hygiene.
• Personnel should not be permitted to eat, drink, smoke
or apply cosmetic in animal rooms.

15. TRANSPORT OF LABORATORY ANIMALS
• The main considerations for transport of animals are
• the mode of transport,
• the containers, the animal density in cages, food and
water during transit,
• protection from transit infection, injuries and stress.

17. LABORATORY ANIMAL
• HUSBANDARY & MANAGEMENT

18. PHYSICAL FACILITIES
• BUILDING MATERIALS- moisture-proof, fire-resistant,
seamless materials are most desirable for interior surfaces
including vermin and pest resistance.
• CORRIDOR- wide enough to facilitate the movement of
personnel as well as equipments and should be kept clean.
• UTILITIES- water lines, drain pipes and electrical connection
• ANIMAL ROOM DOORS- rust, vermin and dust proof. it
properly within their frames and provided with an observation
window.
• FLOORS- smooth, moisture proof, non-absorbent, skid-proof,.

19. DRAINS
floor drains are not essential in all rooms used exclusively for
housing rodents.
• WALLS & CEILINGS-
free of cracks, unsealed utility penetrations, or imperfect junction
with doors, ceilings, floors and corners.
• STORAGE AREAS- separate storage areas should be
designed for feed, bedding, cages and materials not in use.
• FACILITIES FOR SANITIZING EQUIPMET AND SUPPLIES-
an area for sanitizing cages and ancillary equipment is
essential with adequate water supply.
• EXPERIMENTAL AREA- should be carried out in a separate
area from the place where animals are housed.

20. ENVIRONMENT
• Air conditioning is for laboratory animals. temperature
with in the range of 180 -290c
relative humidity- 30-70% throughout the year
• for large animal comfortable zone-18-37°˚c
POWER & LIGHTING-
the electrical system should be safe and provide appropriate
lighting and a sufficient no. of power outlets.
A time control light system should be used.
NOISE CONTROL- noise free environment

21. ANIMAL HUSBANDARY
• CAGING & HOUSING SYSTEM-
 Adequate ventilation
 Meet the biological need of animal
 Keep the animal dry and clean
 Cages made of steel or painted steel
 Feeding and watering devices should be easily
accessible for filing, changing, cleaning and servicing.

22. FOOD
• Should be fed palatable, non-contaminated and
nutritionally adequate food.
• Diet should be free from heavy metals.
BEDDING
 Absorbent, free of toxic chemicals or other substances
that could injure animals or personnel
 Should be removed and replaced with fresh materials as
often as necessary to keep animal clean and dry.

23. WATER
• Ordinarily animals should have continuous access to
fresh, potable, uncontaminated
• drinking water, according to their particular requirements.
SANITATION CLEANLIESS
• Sanitation is essential in an animal facility. Animal rooms,
corridors, storage spaces, and
• other areas should be cleaned with appropriate
detergents and disinfectant .

24. Waste disposal
• Wastes should be removed regularly and frequently. All
waste should be collected and
• disposed in a -safe and sanitary manner. The most
preferred method of waste disposal is incineration.
EMERGENCY, WEEKEND AND HOLIDAY CARE
• Animal should be cared for by qualified personnel every
day, including weekends and holidays, to safeguards
their well- being including emergency veterinary care

25. Record keeping
The Animal House should maintain following records:
1. Animal House plans, which includes typical floor plan, all fixtures
etc.
2. Animal House staff record - both technical and non - technical
3. Health record of staff! animals.
4. All SOPs relevant to the animals
5. Breeding, stock, purchase and sales records
6. Minutes of institute Animals Ethics Committee Meetings
7. Records of experiments conducted with the number of animals
used (copy of Form b)
8. Death Record
9. Clinical record of sick animals .
10. Training record of staff involved

27. ANAESTHESIA
EUTHANASIA
ANAESTHESIA
• It must also be ensured that the anesthesia is given for
the full duration of experiment.
• and at no stage the animal is conscious to perceive pain
during the experiment.
• sedatives, analgesics and anaesthetics should be used
to control pain or distress under experiment

29. Euthanasia
(eu =good.thanatos =death)
PURPOSE
• End of experiment , to provide tissue for scientific
purpose.
• Free the animal of pain
• Diseased animal or animal in bad condition

30. Methods
• Physical methods
• electrocution , exanguination , decaptation , cervical
dislocation
• Drug administration
overdose of chloral hydrate, ketamine , chloroform

32. References
• icmr.nic.in/bioethics/final
• cpcsea.nic.in
• www.aaalac.org/resources/SOP
• Google.com

33. Thank you