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The development of sensitive spectrophotometer able to measure a wide range of wavelengths has allowed natural substrates and measurements in the ultraviolet region of the spectrum to be used in clinical tests. Assays using natural substrates and measurements in the ultraviolet region are the basis of most of the commonly used methods to determine enzyme activities in clinical biochemistry. Typically, they continuously measure the absorbances of NAD+ or NADP+ at 340 nm. Figure shows the reactions used to monitor the activities of creatine kinase (CK) in clinical laboratories by measuring changes in the absorbance of NAD+ or NADP+. Clinical investigations using this enzyme can be used to detect muscle damage..
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