Novel Resistance to Rust Pathogens in Bread Wheat

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Borlaug Global Rust Initiative

Li Huang, Montana State University

Technology Health & Medicine

Mutagenesis

Alpowa--Susceptible line Leaf rust screening

Treat seeds with EMS Select for resistance ones

Mutation density: 1/12 kb (Feiz et al., 2009, Genetics)

Al-180 Al-220 Al-268 Al-271 Al-535
Alpowa MNs MNs MNs MNs MNs

APR
Pt S R R R R R
S R R R S S
Pgt
R R R R R S
Pst APR
S R R S S S

Infection types of the mutant and the wild type
with Pt race PBJL

3 dpi 6 dpi
Al-220 Al-220
‘Alpowa’ MNs ‘Alpowa’ MNs

Infection types of the mutant and the wild type
with Pt race PBJL at seedling stage
Al-220
‘Alpowa’ MNs

dpi 12 15 18 21 12 15 18 21

Infection types with Pt race PBJL
at adult stage
Al-220
‘Alpowa’ MNs

dpi 18 21 24 18 21 24

Infection types with Pt race PBJL
at seedling stage
Susceptible Resistance

8 11 14 17 20 8 11 14 17 20

Chinese Spring x MNsAl-220

Infection types with Pgt race TMLK
at seedling stage
Al-220
‘Alpowa’ MNs

dpi 18 21 24 18 21 24

Al-220
Infection types of MNs and ‘Alpowa’ with races
of three rust pathogens
Lines
Pathogens Races Stage Al-220
‘Alpowa’ MNs
PBJL Seedling 3~4 ;~2
Adult S R
Pt SBGD Adult S I
BBBDD Adult S I
78 Seedling 8 7
127 Seedling 8 8
Pst Adult R R
101/102 Adult S R
TLMK Seedling 3-~4 ;1+
QFCS 3~4 ;1+
TTKSK 3+ ;1+
TTKST 3+ ;1+
Pgt TTTSK 3+ ;
TPMK 4 ;
TTTT 4 ;1+
QTHJ 3+ ;1+

Infection types with a local Egt race
at seedling stage
Al-220
‘Alpowa’ MNs

dpi 18 21 24 18 21 24

Seedling stage

PR Genes Enzyme Actvity
PR1
PR2 β-1,3-Glucanase
PR3 Endochitinase
PR4 Endochitinase
PR5 Thaumatin-like
PR9 Lignin-forming Peroxidase
PR10 RNase

Adult stage

Al-220
Genetic analysis of resistance in the MNs

F2 individuals
Expected
Crosses Ratio X2
Resistant Susceptible

Al-220
MNs / ‘Alpowa’ 210 71 3:1 0.67

Al-220
‘Chinese Spring’ / MNs 100 36 3:1 0.69

2BS
cM

Centromere

Genetics map of Lr48 Chinese Spring 2B Bin Map
Bansal et al., 2008, TAG Genetic map of MNsAL220 GrainGenes

Acknowledgement
The Huang Lab
Jackie Campbell
Dr. Hongtao Zhang

Collaborators
Dr. Mike Giroux MSU
Dr. Yue Jin USDA-ARS, Cereal Disease Lab
Dr. Meinan Wang WSU
Dr. Xianming Chen WSU
Dr. Deven See WSU

Funding

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Novel Resistance to Rust Pathogens in Bread Wheat

1. Novel resistance to rust pathogens in bread wheat Li Huang Montana State University

2. Mutagenesis Alpowa--Susceptible line Leaf rust screening Treat seeds with EMS Select for resistance ones Mutation density: 1/12 kb (Feiz et al., 2009, Genetics)

3. Al-180 Al-220 Al-268 Al-271 Al-535 Alpowa MNs MNs MNs MNs MNs APR Pt S R R R R R S R R R S S Pgt R R R R R S Pst APR S R R S S S

6. Al-220 ‘Alpowa’ MNs

8. Infection types of the mutant and the wild type with Pt race PBJL 3 dpi 6 dpi Al-220 Al-220 ‘Alpowa’ MNs ‘Alpowa’ MNs

9. Infection types of the mutant and the wild type with Pt race PBJL at seedling stage Al-220 ‘Alpowa’ MNs dpi 12 15 18 21 12 15 18 21

10. Infection types with Pt race PBJL at adult stage Al-220 ‘Alpowa’ MNs dpi 18 21 24 18 21 24

11. Infection types with Pt race PBJL at seedling stage Susceptible Resistance 8 11 14 17 20 8 11 14 17 20 Chinese Spring x MNsAl-220

12. Infection types with Pgt race TMLK at seedling stage Al-220 ‘Alpowa’ MNs dpi 18 21 24 18 21 24

13. Al-220 Infection types of MNs and ‘Alpowa’ with races of three rust pathogens Lines Pathogens Races Stage Al-220 ‘Alpowa’ MNs PBJL Seedling 3~4 ;~2 Adult S R Pt SBGD Adult S I BBBDD Adult S I 78 Seedling 8 7 127 Seedling 8 8 Pst Adult R R 101/102 Adult S R TLMK Seedling 3-~4 ;1+ QFCS 3~4 ;1+ TTKSK 3+ ;1+ TTKST 3+ ;1+ Pgt TTTSK 3+ ; TPMK 4 ; TTTT 4 ;1+ QTHJ 3+ ;1+

14. Infection types with a local Egt race at seedling stage Al-220 ‘Alpowa’ MNs dpi 18 21 24 18 21 24

15. Seedling stage PR Genes Enzyme Actvity PR1 PR2 β-1,3-Glucanase PR3 Endochitinase PR4 Endochitinase PR5 Thaumatin-like PR9 Lignin-forming Peroxidase PR10 RNase Adult stage

16. Al-220 MNs ‘Alpowa’

17. Al-220 Genetic analysis of resistance in the MNs F2 individuals Expected Crosses Ratio X2 Resistant Susceptible Al-220 MNs / ‘Alpowa’ 210 71 3:1 0.67 Al-220 ‘Chinese Spring’ / MNs 100 36 3:1 0.69

18. 2BS cM Centromere Genetics map of Lr48 Chinese Spring 2B Bin Map Bansal et al., 2008, TAG Genetic map of MNsAL220 GrainGenes

19. Acknowledgement The Huang Lab Jackie Campbell Dr. Hongtao Zhang Collaborators Dr. Mike Giroux MSU Dr. Yue Jin USDA-ARS, Cereal Disease Lab Dr. Meinan Wang WSU Dr. Xianming Chen WSU Dr. Deven See WSU Funding

Novel Resistance to Rust Pathogens in Bread Wheat

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