Metabolomics and Discovery of Drugs

1. Metabolomics and Drug Discovery © All rights reserved P. Paulsharma Chakravarthy, School of Genomics, Loyola College www.milser.co.in [email_address]

8. Study of Metabolome - In terms of sequence of reactions by which a specific nutrients is converted to end products, and the energetics of these reaction. - In terms of the mechanisms by which each intermediate is converted to its successor. Such an analysis requires the isolation and characterization of the specific enzymes that catalyze the reactions - In terms of the control mechanisms that regulate the flow of metabolites through the pathway.

10. Metabolic Pathway Databases

11. Representation of Pathways…

12. Gene Information - KEGG

13. Genome Map Representation - KEGG

18. Isoprenoids

19. Screening for Drug Target Pathways

20. DOXP and Mevalonate Pathway

21. Screening for Drug Target Pathways

22. + + + + + Pseudomonas aeruginosa + + + + + Haemophilus influenzae + + + + + Escherichia coli + + + + + Vibrio cholerae + + + + + Bordetella bronchisceptica + + + + + Bordetella pertussis + + + + + Neisseria meningitidis + + + + + Caulobacter crescentus + + + + + Rhodopseudomonas palustris Ych B Ygb B YgbP DXR DXS Organisms

23. + + + + + Helicobacter pylori + + + + + Campylobacter jejuni + + + + + Erwinia carotovora + + + + + Yersinia pestis + + + + + Pasteurella multocida + + + + + Salmonella typhimurium + + + + + Salmonella typhi -- -- -- + -- Pseudomonas fluorescens + + + + + Pseudomonas putida

24. Thank you… [email_address]