SlideShare a Scribd company logo
1 of 29
Download to read offline
1
IR and FT-IR
Presented By,
Chetan M. Jain
First Year M.Pharm
(Quality Assurance)
Government College of Pharmacy,Amravati.
11/12/16
InFRaRed
SpecTRoScopy
 Intoroduction
2
Provide useful information about structure of molecule.
Absorption of IR radiation by molecule and exhibit absorption
spectra.
Change in dipole moment.
Energy of radiation is directly proportional to wave number and
inversely proportional to wavelength.
Region Wavelength (µ) Wave number (cm-1
)
Near IR 0.75-2.5 1200-4000
Infrared 2.5-16 4000-625
Far IR 16-200 625-10 11/12/16
Chetan Jain
oRIgIn oF IR SpecTRa
 UV- Visible spectrum results in changes of electronic as
well as vibrational and rotational level in molecule.
 IR radiation shows only vibrational and rotational level.
 Vibrational-Rotational spectrum.
3 11/12/16Chetan Jain
4
11/12/16Chetan Jain
InSTRumenTaTIon
The optical components of IR spectrophotometer are
5
Light Source
Monochromator
Sample Holder
Detector
Recorder
11/12/16Chetan Jain
Dispersive instruments
FT-IR instrument
Non-dispersive instruments
IR RadIaTIon SouRce
 Hot bodies continuously emmitting radiation
 Incandescent lamp
 Nernst Glover
6
A closed wound nichrome wire is used.
A black oxide film formed on the coil gives emissivity by heating.
Temp. can be raised to 1100°C.
Used in near IR region and failed in IR region.
In IR region – most common source.
Consists of hollow rod-2mm in dia and 2-5cm in length.
Composed of mixture of oxides like zirconium, yttrium and thorium.
Temp: 1000-1800°C.
11/12/16Chetan Jain
 Advantages of Nernst glower
 Disadvantage
7
It emits IR radiation over wide range of wavelength.
The intensity of radiation maintains steady and constant over long period
of time.
Used in air as it is not oxidized.
Frequent mechanical failure.
11/12/16Chetan Jain
 Globar Source
 Mercury Arc
 Tungsten Filament lamp
8
It is rod of sintered silicon carbide about 5cm in length and 4mm in
diameter
Temp- 1300°C.
 If radiation above 10µ it is used.
Far IR region it is used.
It is encolosed in quartz jacket to reduce loss.
Useful for near IR region only.
11/12/16Chetan Jain
MonochroMators
 Select desired frequencies and reject other frequencies.
 These are of two types
9
1) Prism Monochromator
2) Grating Monochromator
11/12/16Chetan Jain
PrisM
MonochroMator
 Greater range and simplicity.
 Halogens salts are used.
 Crystalline NaCl: 5-15µ
 Crystalline KBr/CBr: 15-40 µ
 LiF : 0.75-2.5
 Two types
10
Single beam monochromator
Double beam Monochromator
11/12/16Chetan Jain
GratinG MonochroMator
 Series of parallel straight lines cut into plane
surface.
 Constructed from glass coated with aluminium.
 Gives radiation into single order.
 Advantages
11
Not attacked by moisture.
Used over considerable wavelength.
Sturdy and long lasting.
11/12/16Chetan Jain
saMPle holders and saMPlin
of substance
 Used for solid, liquid and gas.
 Material containing sample must be transperent to
IR region.
 Sample cell constructed from rock salt.
 Sample must be pure and free from water.
12 11/12/16Chetan Jain
saMPlinG of solids
 Solid dissolved in solvent
 As Solid Film
 Mull technique
13
Carbon tetrachloride,Chloroform,acetone and cyclohexane.
Sample solution is placed on surface of KBr or Nacl and
allowed to evaporate.
Solid sample mixed with nujol to form a paste. This paste is
then sandwiched between two salt plates and used for spectral
measurement.
Nujol has absorption at 2915,1462,1376 and 719cm-1
11/12/16Chetan Jain
 Pressed Pellet Technique(Disk Method)
 Advatages of pellet tech. over mull tech. is
14
The small amount of finally ground solid sample is mixed with
100 times its weight of powdered KBr.
This mixture is pressed under high pressure in IR tablet press
to form pellet.
Eliminates the problem of band
If KBr pellet is preserved can be reused
Resolution of KBr is superior
11/12/16Chetan Jain
saMPlinG of liquids
 For sampling of various cell like sandwitch cell,demountable cell
and cavity cell are used.
 Made up of Nacl, KBr or thallium bromide.
 Demountable cell:
 Cavity cell:
15
It consists of pair of salt plates separeted by gasket made of
metal or teflon and together by an clamp.
Matching a parallel-sided hole in a salt block makes it.
11/12/16Chetan Jain
saMPlinG of Gas
 The dried gases introduced via a stop cock.
 The gas sample is introduced into the gas cell made up of
glass or metal cylinder of about 10cm long.
 The end walls of the gas cell made up of NaCl.
16 11/12/16Chetan Jain
Detectors
 Two types of detectors are used
 Thermal detectors
17
Thermal detectors
Photodetectors
When IR radiation falls on these detectors they cause heating
which give rise to P.D. This P.D. is depends upon amt of
radiation. Thermocouple
Bolometer
Thermister
Golay cell 11/12/16Chetan Jain
 Thermocouple
18
These are dissimilar strips of metal join together at one end.
Two fine wires of metals are welded with blackened gold foil,
which absorbs radiation.
One welded joint(Cold junction) is kept at constant temp.
The other welded joint (Hot junction) is exposed to radiation.
This exposure of hot junction causes rise in its temp, cause the
P.D. proportional to the amt of radiation.
11/12/16Chetan Jain
 Bolometers
19
These are constructed from metals or semiconductors.
When IR radiation falls on bolometer its temp. changes cause
change in the resistence of the conductor.
This change in the resistence of conductor is depends upon amt
of radiation.
This made one arm of Wheistone Bridge.
If IR radiation falls on the bolometer, the bridge becomes
unbalanced due to change in the electrical resistence causes
current flow through galvanometer.this amt of current flow is a
measure of intensity of radiation.
11/12/16Chetan Jain
 Thermister
20
These function similar to bolometer.
These are the resisters made by fusing several metallic
oxides.
11/12/16Chetan Jain
 Golay cell
21
It consists of metal cylinder.
One end of which closed by balanced metal plate and other with
metalised diaphragm.
Cylinder is filled with gas like xenon.
When the radiation falls on blackened metal plate it is heated which
causes expansion of gas, which affect the motion of diaphragm.
11/12/16Chetan Jain
Photon Detectors
 Used in near IR region.
 Consists of semiconductors like lead sulphide, lead
telluride.
 When radiation are fall on these they goes to higher
level and produce signal.
 Photoconductivity cell
22
It consists of thin layer of lead sulphide supported on glass
and enclosed into an evacuated glass envelope.
11/12/16Chetan Jain
Fourier transForm ir
sPectroscoPy (Ftir)
23 11/12/16Chetan Jain
Fourier Transform IR
Spectroscopy (FTIR)
 Advanced technique.
 Interferometer(Michelson Interferometer).
24 11/12/16Chetan Jain
 Advantages
25
Fellgett Advantage
Jacquinot advantage
11/12/16Chetan Jain
InterpretatIon of Ir Spectr
26 11/12/16Chetan Jain
applIcatIonS of Ir and ft
Ir
 Qualitative application
 Quantitative application.
27 11/12/16Chetan Jain
References
 Pharamceutical analysis by A.V. Kasture, S.G. Wadodkar,K.R.
Mahadik, H.N. More, Volume II, Nirali Prakashan, Eleventh
edition (2004), p.no. 207-221.
 Fundamental of analytical chemistry by D.A.Skoog,
D.M.West,F.J.Holler,7th
edition p.no.592-597.
 Organic Spectroscopy by William Kemp, 3rd
edition, Palgrave
Publication, p.no19-56.
 Instrumental Methods of Analysis by H.A.Willard, L.L.Merritt,
J.A.Dean, F.A. Settle, 7th
edition, p.no. 288 to 320.
28 11/12/16Chetan Jain
29 11/12/16Chetan Jain

More Related Content

What's hot

IR Spectroscopy - Sudheerkumar Kamarapu, M. Pharmacy Lecture pdf
IR Spectroscopy - Sudheerkumar Kamarapu, M. Pharmacy Lecture pdfIR Spectroscopy - Sudheerkumar Kamarapu, M. Pharmacy Lecture pdf
IR Spectroscopy - Sudheerkumar Kamarapu, M. Pharmacy Lecture pdfDr. Sudheer Kumar Kamarapu
 
Mass spectrometry and ionization techniques
Mass spectrometry and ionization techniquesMass spectrometry and ionization techniques
Mass spectrometry and ionization techniquesSurbhi Narang
 
Relaxation in NMR
Relaxation in NMR Relaxation in NMR
Relaxation in NMR RM42
 
Instrumentation of IR spectroscopy
Instrumentation of IR spectroscopyInstrumentation of IR spectroscopy
Instrumentation of IR spectroscopyTalha Liaqat
 
Ionizaion Techniques - Mass Spectroscopy
Ionizaion Techniques - Mass SpectroscopyIonizaion Techniques - Mass Spectroscopy
Ionizaion Techniques - Mass SpectroscopySuraj Choudhary
 
Factors and applications of IR Spectroscopy
Factors and applications of IR SpectroscopyFactors and applications of IR Spectroscopy
Factors and applications of IR SpectroscopyKasturi Banerjee
 
Solvents and solvent effect in UV - Vis Spectroscopy, By Dr. Umesh Kumar sh...
Solvents and  solvent effect in UV -  Vis Spectroscopy, By Dr. Umesh Kumar sh...Solvents and  solvent effect in UV -  Vis Spectroscopy, By Dr. Umesh Kumar sh...
Solvents and solvent effect in UV - Vis Spectroscopy, By Dr. Umesh Kumar sh...Dr. UMESH KUMAR SHARMA
 
Factors affecting fluorescence intensity(pharmaceutical analysis)
Factors affecting fluorescence intensity(pharmaceutical analysis)Factors affecting fluorescence intensity(pharmaceutical analysis)
Factors affecting fluorescence intensity(pharmaceutical analysis)MdIrfanUddin2
 
Spin spin coupling and coupling constant
Spin spin coupling and coupling constantSpin spin coupling and coupling constant
Spin spin coupling and coupling constantHimal Barakoti
 
I R spectroscopy & its application
I R spectroscopy & its application I R spectroscopy & its application
I R spectroscopy & its application Amit Agnihotri
 
Instrumentation IR Spectroscopy: Detectors
Instrumentation IR Spectroscopy: DetectorsInstrumentation IR Spectroscopy: Detectors
Instrumentation IR Spectroscopy: DetectorsVrushali Tambe
 
UV-Visible Spectroscopy
UV-Visible SpectroscopyUV-Visible Spectroscopy
UV-Visible SpectroscopySudha Reddy
 
Quadrupole and Time of Flight Mass analysers.
 Quadrupole and Time of Flight Mass analysers. Quadrupole and Time of Flight Mass analysers.
Quadrupole and Time of Flight Mass analysers.Gagangowda58
 

What's hot (20)

Coupling constant
Coupling constantCoupling constant
Coupling constant
 
IR Spectroscopy - Sudheerkumar Kamarapu, M. Pharmacy Lecture pdf
IR Spectroscopy - Sudheerkumar Kamarapu, M. Pharmacy Lecture pdfIR Spectroscopy - Sudheerkumar Kamarapu, M. Pharmacy Lecture pdf
IR Spectroscopy - Sudheerkumar Kamarapu, M. Pharmacy Lecture pdf
 
Mass spectrometry and ionization techniques
Mass spectrometry and ionization techniquesMass spectrometry and ionization techniques
Mass spectrometry and ionization techniques
 
Relaxation in NMR
Relaxation in NMR Relaxation in NMR
Relaxation in NMR
 
Effect of solvent
Effect of solventEffect of solvent
Effect of solvent
 
FTIR spectrophotometer
FTIR spectrophotometerFTIR spectrophotometer
FTIR spectrophotometer
 
Instrumentation of IR spectroscopy
Instrumentation of IR spectroscopyInstrumentation of IR spectroscopy
Instrumentation of IR spectroscopy
 
Ionizaion Techniques - Mass Spectroscopy
Ionizaion Techniques - Mass SpectroscopyIonizaion Techniques - Mass Spectroscopy
Ionizaion Techniques - Mass Spectroscopy
 
Factors and applications of IR Spectroscopy
Factors and applications of IR SpectroscopyFactors and applications of IR Spectroscopy
Factors and applications of IR Spectroscopy
 
Solvents and solvent effect in UV - Vis Spectroscopy, By Dr. Umesh Kumar sh...
Solvents and  solvent effect in UV -  Vis Spectroscopy, By Dr. Umesh Kumar sh...Solvents and  solvent effect in UV -  Vis Spectroscopy, By Dr. Umesh Kumar sh...
Solvents and solvent effect in UV - Vis Spectroscopy, By Dr. Umesh Kumar sh...
 
Factors affecting fluorescence intensity(pharmaceutical analysis)
Factors affecting fluorescence intensity(pharmaceutical analysis)Factors affecting fluorescence intensity(pharmaceutical analysis)
Factors affecting fluorescence intensity(pharmaceutical analysis)
 
Mass spectroscopy
Mass spectroscopyMass spectroscopy
Mass spectroscopy
 
Nmr instrumentation
Nmr  instrumentationNmr  instrumentation
Nmr instrumentation
 
Spin spin coupling and coupling constant
Spin spin coupling and coupling constantSpin spin coupling and coupling constant
Spin spin coupling and coupling constant
 
I R spectroscopy & its application
I R spectroscopy & its application I R spectroscopy & its application
I R spectroscopy & its application
 
Instrumentation IR Spectroscopy: Detectors
Instrumentation IR Spectroscopy: DetectorsInstrumentation IR Spectroscopy: Detectors
Instrumentation IR Spectroscopy: Detectors
 
UV-Visible Spectroscopy
UV-Visible SpectroscopyUV-Visible Spectroscopy
UV-Visible Spectroscopy
 
Quadrupole and Time of Flight Mass analysers.
 Quadrupole and Time of Flight Mass analysers. Quadrupole and Time of Flight Mass analysers.
Quadrupole and Time of Flight Mass analysers.
 
Fluorometry
FluorometryFluorometry
Fluorometry
 
C 13 NMR Spectroscopy
C 13 NMR SpectroscopyC 13 NMR Spectroscopy
C 13 NMR Spectroscopy
 

Viewers also liked

Infrared spectroscopy
Infrared spectroscopyInfrared spectroscopy
Infrared spectroscopyNida Ashraf
 
Applications of IR (Infrared) Spectroscopy in Pharmaceutical Industry
Applications of IR (Infrared) Spectroscopy in Pharmaceutical IndustryApplications of IR (Infrared) Spectroscopy in Pharmaceutical Industry
Applications of IR (Infrared) Spectroscopy in Pharmaceutical Industrywonderingsoul114
 
Basics of infrared spectroscopy
Basics of infrared spectroscopyBasics of infrared spectroscopy
Basics of infrared spectroscopyNijas Mohamed
 
FT-IR spectroscopy Instrumentation and Application, By- Anubhav singh, M.pharm
FT-IR spectroscopy Instrumentation and Application, By- Anubhav singh, M.pharmFT-IR spectroscopy Instrumentation and Application, By- Anubhav singh, M.pharm
FT-IR spectroscopy Instrumentation and Application, By- Anubhav singh, M.pharmAnubhav Singh
 
Introduction and Principle of IR spectroscopy
Introduction and Principle of IR spectroscopyIntroduction and Principle of IR spectroscopy
Introduction and Principle of IR spectroscopyRajaram Kshetri
 
Ultra violet (UV) spectroscopy, introduction,principle instrumentation,differ...
Ultra violet (UV) spectroscopy, introduction,principle instrumentation,differ...Ultra violet (UV) spectroscopy, introduction,principle instrumentation,differ...
Ultra violet (UV) spectroscopy, introduction,principle instrumentation,differ...mariomS7
 
Infrared spectroscopy
Infrared spectroscopyInfrared spectroscopy
Infrared spectroscopyRevti Lohey
 
Introduction and applications of FT- IR spectroscopy
Introduction and applications of FT- IR spectroscopyIntroduction and applications of FT- IR spectroscopy
Introduction and applications of FT- IR spectroscopyKANDASAMY K
 
How to Create a Slideshare Account
How to Create a Slideshare AccountHow to Create a Slideshare Account
How to Create a Slideshare AccountAna Villarmente
 
Theory of IR spectroscopy
Theory of IR spectroscopyTheory of IR spectroscopy
Theory of IR spectroscopychiranjibi68
 

Viewers also liked (20)

IR SPECTROSCOPY
IR SPECTROSCOPYIR SPECTROSCOPY
IR SPECTROSCOPY
 
Infrared spectroscopy
Infrared spectroscopyInfrared spectroscopy
Infrared spectroscopy
 
UV visible spectroscopy
UV visible spectroscopyUV visible spectroscopy
UV visible spectroscopy
 
ir spectroscopy
ir spectroscopyir spectroscopy
ir spectroscopy
 
Applications of IR (Infrared) Spectroscopy in Pharmaceutical Industry
Applications of IR (Infrared) Spectroscopy in Pharmaceutical IndustryApplications of IR (Infrared) Spectroscopy in Pharmaceutical Industry
Applications of IR (Infrared) Spectroscopy in Pharmaceutical Industry
 
Basics of infrared spectroscopy
Basics of infrared spectroscopyBasics of infrared spectroscopy
Basics of infrared spectroscopy
 
FT-IR spectroscopy Instrumentation and Application, By- Anubhav singh, M.pharm
FT-IR spectroscopy Instrumentation and Application, By- Anubhav singh, M.pharmFT-IR spectroscopy Instrumentation and Application, By- Anubhav singh, M.pharm
FT-IR spectroscopy Instrumentation and Application, By- Anubhav singh, M.pharm
 
Introduction and Principle of IR spectroscopy
Introduction and Principle of IR spectroscopyIntroduction and Principle of IR spectroscopy
Introduction and Principle of IR spectroscopy
 
Ultra violet (UV) spectroscopy, introduction,principle instrumentation,differ...
Ultra violet (UV) spectroscopy, introduction,principle instrumentation,differ...Ultra violet (UV) spectroscopy, introduction,principle instrumentation,differ...
Ultra violet (UV) spectroscopy, introduction,principle instrumentation,differ...
 
Infrared absorption spectroscopy
Infrared absorption spectroscopyInfrared absorption spectroscopy
Infrared absorption spectroscopy
 
Ft ir instrument
Ft ir instrumentFt ir instrument
Ft ir instrument
 
Ft ir
Ft irFt ir
Ft ir
 
Infra red spectroscopy.
Infra red spectroscopy.Infra red spectroscopy.
Infra red spectroscopy.
 
Infrared spectroscopy
Infrared spectroscopyInfrared spectroscopy
Infrared spectroscopy
 
Introduction and applications of FT- IR spectroscopy
Introduction and applications of FT- IR spectroscopyIntroduction and applications of FT- IR spectroscopy
Introduction and applications of FT- IR spectroscopy
 
Ir spectroscopy
Ir spectroscopyIr spectroscopy
Ir spectroscopy
 
How to Create a Slideshare Account
How to Create a Slideshare AccountHow to Create a Slideshare Account
How to Create a Slideshare Account
 
Theory of IR spectroscopy
Theory of IR spectroscopyTheory of IR spectroscopy
Theory of IR spectroscopy
 
Hplc review
Hplc reviewHplc review
Hplc review
 
Ir spectroscopy an introduction
Ir spectroscopy   an introductionIr spectroscopy   an introduction
Ir spectroscopy an introduction
 

Similar to IR and FT-IR Spectroscopy

Instrumentation of infrared spectroscopy
Instrumentation of infrared spectroscopyInstrumentation of infrared spectroscopy
Instrumentation of infrared spectroscopyANKITHRAI4
 
Mass spec and thermal methods of analysis - By France Chavangwane
Mass spec and thermal methods of analysis - By France ChavangwaneMass spec and thermal methods of analysis - By France Chavangwane
Mass spec and thermal methods of analysis - By France ChavangwaneFranceChavangwane
 
M.Sc. Part I 1.3 INFRARED SPECTROPHOTOMETRY PPT (1).ppsx
M.Sc. Part I  1.3 INFRARED SPECTROPHOTOMETRY PPT (1).ppsxM.Sc. Part I  1.3 INFRARED SPECTROPHOTOMETRY PPT (1).ppsx
M.Sc. Part I 1.3 INFRARED SPECTROPHOTOMETRY PPT (1).ppsxSIMARANJAISWAL41M3S
 
EVALUATION SEMINAR ON IR INSTRUMENTATION
EVALUATION SEMINAR ON IR INSTRUMENTATIONEVALUATION SEMINAR ON IR INSTRUMENTATION
EVALUATION SEMINAR ON IR INSTRUMENTATIONnivedithag131
 
X - RAY DIFFRACTION TECHNIQUE
X - RAY DIFFRACTION TECHNIQUEX - RAY DIFFRACTION TECHNIQUE
X - RAY DIFFRACTION TECHNIQUEEknath Babu T.B.
 
Material technology Newly develpoed engineering materials
Material technology Newly develpoed engineering materialsMaterial technology Newly develpoed engineering materials
Material technology Newly develpoed engineering materialsMihir Taylor
 
INSTRUMENTATION OF UV-VISIBLE SPECTROSCOPY
INSTRUMENTATION OF UV-VISIBLE SPECTROSCOPYINSTRUMENTATION OF UV-VISIBLE SPECTROSCOPY
INSTRUMENTATION OF UV-VISIBLE SPECTROSCOPYCh. Bhargava krishna
 
xray diffraction instrumentation
xray diffraction instrumentationxray diffraction instrumentation
xray diffraction instrumentationBindu Kshtriya
 
Ir spectroscopy instrumentation, b y -dr. umesh kumar sharma and arathy s a
Ir spectroscopy instrumentation, b y -dr. umesh kumar sharma and arathy s aIr spectroscopy instrumentation, b y -dr. umesh kumar sharma and arathy s a
Ir spectroscopy instrumentation, b y -dr. umesh kumar sharma and arathy s aDr. UMESH KUMAR SHARMA
 
Field Ion - Infrared- Raman Spectroscopy
Field Ion - Infrared- Raman SpectroscopyField Ion - Infrared- Raman Spectroscopy
Field Ion - Infrared- Raman SpectroscopySAMEER VISHWAKARMA
 
UV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptx
UV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptxUV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptx
UV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptxDr. Vijaya Barge
 
Infrared spectroscopy
Infrared spectroscopy Infrared spectroscopy
Infrared spectroscopy PV. Viji
 
instrupres-140329002030-phpapp01 (1).pdf
instrupres-140329002030-phpapp01 (1).pdfinstrupres-140329002030-phpapp01 (1).pdf
instrupres-140329002030-phpapp01 (1).pdfSHREYAL7
 
Chapter 3 detection devices
Chapter 3 detection devicesChapter 3 detection devices
Chapter 3 detection devicesROBERT ESHUN
 

Similar to IR and FT-IR Spectroscopy (20)

Instrumentation of infrared spectroscopy
Instrumentation of infrared spectroscopyInstrumentation of infrared spectroscopy
Instrumentation of infrared spectroscopy
 
Mass spec and thermal methods of analysis - By France Chavangwane
Mass spec and thermal methods of analysis - By France ChavangwaneMass spec and thermal methods of analysis - By France Chavangwane
Mass spec and thermal methods of analysis - By France Chavangwane
 
M.Sc. Part I 1.3 INFRARED SPECTROPHOTOMETRY PPT (1).ppsx
M.Sc. Part I  1.3 INFRARED SPECTROPHOTOMETRY PPT (1).ppsxM.Sc. Part I  1.3 INFRARED SPECTROPHOTOMETRY PPT (1).ppsx
M.Sc. Part I 1.3 INFRARED SPECTROPHOTOMETRY PPT (1).ppsx
 
IR spectroscopy
IR spectroscopyIR spectroscopy
IR spectroscopy
 
EVALUATION SEMINAR ON IR INSTRUMENTATION
EVALUATION SEMINAR ON IR INSTRUMENTATIONEVALUATION SEMINAR ON IR INSTRUMENTATION
EVALUATION SEMINAR ON IR INSTRUMENTATION
 
X - RAY DIFFRACTION TECHNIQUE
X - RAY DIFFRACTION TECHNIQUEX - RAY DIFFRACTION TECHNIQUE
X - RAY DIFFRACTION TECHNIQUE
 
Material technology Newly develpoed engineering materials
Material technology Newly develpoed engineering materialsMaterial technology Newly develpoed engineering materials
Material technology Newly develpoed engineering materials
 
INSTRUMENTATION OF UV-VISIBLE SPECTROSCOPY
INSTRUMENTATION OF UV-VISIBLE SPECTROSCOPYINSTRUMENTATION OF UV-VISIBLE SPECTROSCOPY
INSTRUMENTATION OF UV-VISIBLE SPECTROSCOPY
 
xray diffraction instrumentation
xray diffraction instrumentationxray diffraction instrumentation
xray diffraction instrumentation
 
Ir spectroscopy instrumentation, b y -dr. umesh kumar sharma and arathy s a
Ir spectroscopy instrumentation, b y -dr. umesh kumar sharma and arathy s aIr spectroscopy instrumentation, b y -dr. umesh kumar sharma and arathy s a
Ir spectroscopy instrumentation, b y -dr. umesh kumar sharma and arathy s a
 
Infrared spectroscopy
Infrared spectroscopyInfrared spectroscopy
Infrared spectroscopy
 
Field Ion - Infrared- Raman Spectroscopy
Field Ion - Infrared- Raman SpectroscopyField Ion - Infrared- Raman Spectroscopy
Field Ion - Infrared- Raman Spectroscopy
 
TYBSc :Types of LASER
TYBSc :Types of LASERTYBSc :Types of LASER
TYBSc :Types of LASER
 
REU final poster
REU final posterREU final poster
REU final poster
 
UV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptx
UV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptxUV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptx
UV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptx
 
Infrared spectroscopy
Infrared spectroscopy Infrared spectroscopy
Infrared spectroscopy
 
instrupres-140329002030-phpapp01 (1).pdf
instrupres-140329002030-phpapp01 (1).pdfinstrupres-140329002030-phpapp01 (1).pdf
instrupres-140329002030-phpapp01 (1).pdf
 
Radiopharmaceuticals 2
Radiopharmaceuticals 2Radiopharmaceuticals 2
Radiopharmaceuticals 2
 
Chapter 3 detection devices
Chapter 3 detection devicesChapter 3 detection devices
Chapter 3 detection devices
 
Raman spectroscopy
Raman spectroscopyRaman spectroscopy
Raman spectroscopy
 

Recently uploaded

Contracts with Interdependent Preferences (2)
Contracts with Interdependent Preferences (2)Contracts with Interdependent Preferences (2)
Contracts with Interdependent Preferences (2)GRAPE
 
CW marking grid Analytical BS - M Ahmad.docx
CW  marking grid Analytical BS - M Ahmad.docxCW  marking grid Analytical BS - M Ahmad.docx
CW marking grid Analytical BS - M Ahmad.docxmarwaahmad357
 
Lehninger_Chapter 17_Fatty acid Oxid.ppt
Lehninger_Chapter 17_Fatty acid Oxid.pptLehninger_Chapter 17_Fatty acid Oxid.ppt
Lehninger_Chapter 17_Fatty acid Oxid.pptSachin Teotia
 
Main Exam Applied biochemistry final year
Main Exam Applied biochemistry final yearMain Exam Applied biochemistry final year
Main Exam Applied biochemistry final yearmarwaahmad357
 
biosynthesis of the cell wall and antibiotics
biosynthesis of the cell wall and antibioticsbiosynthesis of the cell wall and antibiotics
biosynthesis of the cell wall and antibioticsSafaFallah
 
3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...
3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...
3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...PirithiRaju
 
Legacy Analysis of Dark Matter Annihilation from the Milky Way Dwarf Spheroid...
Legacy Analysis of Dark Matter Annihilation from the Milky Way Dwarf Spheroid...Legacy Analysis of Dark Matter Annihilation from the Milky Way Dwarf Spheroid...
Legacy Analysis of Dark Matter Annihilation from the Milky Way Dwarf Spheroid...Sérgio Sacani
 
RCPE terms and cycles scenarios as of March 2024
RCPE terms and cycles scenarios as of March 2024RCPE terms and cycles scenarios as of March 2024
RCPE terms and cycles scenarios as of March 2024suelcarter1
 
Alternative system of medicine herbal drug technology syllabus
Alternative system of medicine herbal drug technology syllabusAlternative system of medicine herbal drug technology syllabus
Alternative system of medicine herbal drug technology syllabusPradnya Wadekar
 
geometric quantization on coadjoint orbits
geometric quantization on coadjoint orbitsgeometric quantization on coadjoint orbits
geometric quantization on coadjoint orbitsHassan Jolany
 
Pests of ragi_Identification, Binomics_Dr.UPR
Pests of ragi_Identification, Binomics_Dr.UPRPests of ragi_Identification, Binomics_Dr.UPR
Pests of ragi_Identification, Binomics_Dr.UPRPirithiRaju
 
Krishi Vigyan Kendras - कृषि विज्ञान केंद्र
Krishi Vigyan Kendras - कृषि विज्ञान केंद्रKrishi Vigyan Kendras - कृषि विज्ञान केंद्र
Krishi Vigyan Kendras - कृषि विज्ञान केंद्रKrashi Coaching
 
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdfSUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdfsantiagojoderickdoma
 
TORSION IN GASTROPODS- Anatomical event (Zoology)
TORSION IN GASTROPODS- Anatomical event (Zoology)TORSION IN GASTROPODS- Anatomical event (Zoology)
TORSION IN GASTROPODS- Anatomical event (Zoology)chatterjeesoumili50
 
Pests of Redgram_Identification, Binomics_Dr.UPR
Pests of Redgram_Identification, Binomics_Dr.UPRPests of Redgram_Identification, Binomics_Dr.UPR
Pests of Redgram_Identification, Binomics_Dr.UPRPirithiRaju
 
Bureau of Indian Standards Specification of Shampoo.pptx
Bureau of Indian Standards Specification of Shampoo.pptxBureau of Indian Standards Specification of Shampoo.pptx
Bureau of Indian Standards Specification of Shampoo.pptxkastureyashashree
 
Principles & Formulation of Hair Care Products
Principles & Formulation of Hair Care  ProductsPrinciples & Formulation of Hair Care  Products
Principles & Formulation of Hair Care Productspurwaborkar@gmail.com
 
MARSILEA notes in detail for II year Botany.ppt
MARSILEA  notes in detail for II year Botany.pptMARSILEA  notes in detail for II year Botany.ppt
MARSILEA notes in detail for II year Botany.pptaigil2
 

Recently uploaded (20)

Contracts with Interdependent Preferences (2)
Contracts with Interdependent Preferences (2)Contracts with Interdependent Preferences (2)
Contracts with Interdependent Preferences (2)
 
CW marking grid Analytical BS - M Ahmad.docx
CW  marking grid Analytical BS - M Ahmad.docxCW  marking grid Analytical BS - M Ahmad.docx
CW marking grid Analytical BS - M Ahmad.docx
 
Lehninger_Chapter 17_Fatty acid Oxid.ppt
Lehninger_Chapter 17_Fatty acid Oxid.pptLehninger_Chapter 17_Fatty acid Oxid.ppt
Lehninger_Chapter 17_Fatty acid Oxid.ppt
 
Main Exam Applied biochemistry final year
Main Exam Applied biochemistry final yearMain Exam Applied biochemistry final year
Main Exam Applied biochemistry final year
 
biosynthesis of the cell wall and antibiotics
biosynthesis of the cell wall and antibioticsbiosynthesis of the cell wall and antibiotics
biosynthesis of the cell wall and antibiotics
 
3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...
3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...
3.2 Pests of Sorghum_Identification, Symptoms and nature of damage, Binomics,...
 
Legacy Analysis of Dark Matter Annihilation from the Milky Way Dwarf Spheroid...
Legacy Analysis of Dark Matter Annihilation from the Milky Way Dwarf Spheroid...Legacy Analysis of Dark Matter Annihilation from the Milky Way Dwarf Spheroid...
Legacy Analysis of Dark Matter Annihilation from the Milky Way Dwarf Spheroid...
 
RCPE terms and cycles scenarios as of March 2024
RCPE terms and cycles scenarios as of March 2024RCPE terms and cycles scenarios as of March 2024
RCPE terms and cycles scenarios as of March 2024
 
Alternative system of medicine herbal drug technology syllabus
Alternative system of medicine herbal drug technology syllabusAlternative system of medicine herbal drug technology syllabus
Alternative system of medicine herbal drug technology syllabus
 
geometric quantization on coadjoint orbits
geometric quantization on coadjoint orbitsgeometric quantization on coadjoint orbits
geometric quantization on coadjoint orbits
 
Pests of ragi_Identification, Binomics_Dr.UPR
Pests of ragi_Identification, Binomics_Dr.UPRPests of ragi_Identification, Binomics_Dr.UPR
Pests of ragi_Identification, Binomics_Dr.UPR
 
Cheminformatics tools supporting dissemination of data associated with US EPA...
Cheminformatics tools supporting dissemination of data associated with US EPA...Cheminformatics tools supporting dissemination of data associated with US EPA...
Cheminformatics tools supporting dissemination of data associated with US EPA...
 
Krishi Vigyan Kendras - कृषि विज्ञान केंद्र
Krishi Vigyan Kendras - कृषि विज्ञान केंद्रKrishi Vigyan Kendras - कृषि विज्ञान केंद्र
Krishi Vigyan Kendras - कृषि विज्ञान केंद्र
 
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdfSUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
 
Data delivery from the US-EPA Center for Computational Toxicology and Exposur...
Data delivery from the US-EPA Center for Computational Toxicology and Exposur...Data delivery from the US-EPA Center for Computational Toxicology and Exposur...
Data delivery from the US-EPA Center for Computational Toxicology and Exposur...
 
TORSION IN GASTROPODS- Anatomical event (Zoology)
TORSION IN GASTROPODS- Anatomical event (Zoology)TORSION IN GASTROPODS- Anatomical event (Zoology)
TORSION IN GASTROPODS- Anatomical event (Zoology)
 
Pests of Redgram_Identification, Binomics_Dr.UPR
Pests of Redgram_Identification, Binomics_Dr.UPRPests of Redgram_Identification, Binomics_Dr.UPR
Pests of Redgram_Identification, Binomics_Dr.UPR
 
Bureau of Indian Standards Specification of Shampoo.pptx
Bureau of Indian Standards Specification of Shampoo.pptxBureau of Indian Standards Specification of Shampoo.pptx
Bureau of Indian Standards Specification of Shampoo.pptx
 
Principles & Formulation of Hair Care Products
Principles & Formulation of Hair Care  ProductsPrinciples & Formulation of Hair Care  Products
Principles & Formulation of Hair Care Products
 
MARSILEA notes in detail for II year Botany.ppt
MARSILEA  notes in detail for II year Botany.pptMARSILEA  notes in detail for II year Botany.ppt
MARSILEA notes in detail for II year Botany.ppt
 

IR and FT-IR Spectroscopy

  • 1. 1 IR and FT-IR Presented By, Chetan M. Jain First Year M.Pharm (Quality Assurance) Government College of Pharmacy,Amravati. 11/12/16
  • 2. InFRaRed SpecTRoScopy  Intoroduction 2 Provide useful information about structure of molecule. Absorption of IR radiation by molecule and exhibit absorption spectra. Change in dipole moment. Energy of radiation is directly proportional to wave number and inversely proportional to wavelength. Region Wavelength (µ) Wave number (cm-1 ) Near IR 0.75-2.5 1200-4000 Infrared 2.5-16 4000-625 Far IR 16-200 625-10 11/12/16 Chetan Jain
  • 3. oRIgIn oF IR SpecTRa  UV- Visible spectrum results in changes of electronic as well as vibrational and rotational level in molecule.  IR radiation shows only vibrational and rotational level.  Vibrational-Rotational spectrum. 3 11/12/16Chetan Jain
  • 5. InSTRumenTaTIon The optical components of IR spectrophotometer are 5 Light Source Monochromator Sample Holder Detector Recorder 11/12/16Chetan Jain Dispersive instruments FT-IR instrument Non-dispersive instruments
  • 6. IR RadIaTIon SouRce  Hot bodies continuously emmitting radiation  Incandescent lamp  Nernst Glover 6 A closed wound nichrome wire is used. A black oxide film formed on the coil gives emissivity by heating. Temp. can be raised to 1100°C. Used in near IR region and failed in IR region. In IR region – most common source. Consists of hollow rod-2mm in dia and 2-5cm in length. Composed of mixture of oxides like zirconium, yttrium and thorium. Temp: 1000-1800°C. 11/12/16Chetan Jain
  • 7.  Advantages of Nernst glower  Disadvantage 7 It emits IR radiation over wide range of wavelength. The intensity of radiation maintains steady and constant over long period of time. Used in air as it is not oxidized. Frequent mechanical failure. 11/12/16Chetan Jain
  • 8.  Globar Source  Mercury Arc  Tungsten Filament lamp 8 It is rod of sintered silicon carbide about 5cm in length and 4mm in diameter Temp- 1300°C.  If radiation above 10µ it is used. Far IR region it is used. It is encolosed in quartz jacket to reduce loss. Useful for near IR region only. 11/12/16Chetan Jain
  • 9. MonochroMators  Select desired frequencies and reject other frequencies.  These are of two types 9 1) Prism Monochromator 2) Grating Monochromator 11/12/16Chetan Jain
  • 10. PrisM MonochroMator  Greater range and simplicity.  Halogens salts are used.  Crystalline NaCl: 5-15µ  Crystalline KBr/CBr: 15-40 µ  LiF : 0.75-2.5  Two types 10 Single beam monochromator Double beam Monochromator 11/12/16Chetan Jain
  • 11. GratinG MonochroMator  Series of parallel straight lines cut into plane surface.  Constructed from glass coated with aluminium.  Gives radiation into single order.  Advantages 11 Not attacked by moisture. Used over considerable wavelength. Sturdy and long lasting. 11/12/16Chetan Jain
  • 12. saMPle holders and saMPlin of substance  Used for solid, liquid and gas.  Material containing sample must be transperent to IR region.  Sample cell constructed from rock salt.  Sample must be pure and free from water. 12 11/12/16Chetan Jain
  • 13. saMPlinG of solids  Solid dissolved in solvent  As Solid Film  Mull technique 13 Carbon tetrachloride,Chloroform,acetone and cyclohexane. Sample solution is placed on surface of KBr or Nacl and allowed to evaporate. Solid sample mixed with nujol to form a paste. This paste is then sandwiched between two salt plates and used for spectral measurement. Nujol has absorption at 2915,1462,1376 and 719cm-1 11/12/16Chetan Jain
  • 14.  Pressed Pellet Technique(Disk Method)  Advatages of pellet tech. over mull tech. is 14 The small amount of finally ground solid sample is mixed with 100 times its weight of powdered KBr. This mixture is pressed under high pressure in IR tablet press to form pellet. Eliminates the problem of band If KBr pellet is preserved can be reused Resolution of KBr is superior 11/12/16Chetan Jain
  • 15. saMPlinG of liquids  For sampling of various cell like sandwitch cell,demountable cell and cavity cell are used.  Made up of Nacl, KBr or thallium bromide.  Demountable cell:  Cavity cell: 15 It consists of pair of salt plates separeted by gasket made of metal or teflon and together by an clamp. Matching a parallel-sided hole in a salt block makes it. 11/12/16Chetan Jain
  • 16. saMPlinG of Gas  The dried gases introduced via a stop cock.  The gas sample is introduced into the gas cell made up of glass or metal cylinder of about 10cm long.  The end walls of the gas cell made up of NaCl. 16 11/12/16Chetan Jain
  • 17. Detectors  Two types of detectors are used  Thermal detectors 17 Thermal detectors Photodetectors When IR radiation falls on these detectors they cause heating which give rise to P.D. This P.D. is depends upon amt of radiation. Thermocouple Bolometer Thermister Golay cell 11/12/16Chetan Jain
  • 18.  Thermocouple 18 These are dissimilar strips of metal join together at one end. Two fine wires of metals are welded with blackened gold foil, which absorbs radiation. One welded joint(Cold junction) is kept at constant temp. The other welded joint (Hot junction) is exposed to radiation. This exposure of hot junction causes rise in its temp, cause the P.D. proportional to the amt of radiation. 11/12/16Chetan Jain
  • 19.  Bolometers 19 These are constructed from metals or semiconductors. When IR radiation falls on bolometer its temp. changes cause change in the resistence of the conductor. This change in the resistence of conductor is depends upon amt of radiation. This made one arm of Wheistone Bridge. If IR radiation falls on the bolometer, the bridge becomes unbalanced due to change in the electrical resistence causes current flow through galvanometer.this amt of current flow is a measure of intensity of radiation. 11/12/16Chetan Jain
  • 20.  Thermister 20 These function similar to bolometer. These are the resisters made by fusing several metallic oxides. 11/12/16Chetan Jain
  • 21.  Golay cell 21 It consists of metal cylinder. One end of which closed by balanced metal plate and other with metalised diaphragm. Cylinder is filled with gas like xenon. When the radiation falls on blackened metal plate it is heated which causes expansion of gas, which affect the motion of diaphragm. 11/12/16Chetan Jain
  • 22. Photon Detectors  Used in near IR region.  Consists of semiconductors like lead sulphide, lead telluride.  When radiation are fall on these they goes to higher level and produce signal.  Photoconductivity cell 22 It consists of thin layer of lead sulphide supported on glass and enclosed into an evacuated glass envelope. 11/12/16Chetan Jain
  • 23. Fourier transForm ir sPectroscoPy (Ftir) 23 11/12/16Chetan Jain
  • 24. Fourier Transform IR Spectroscopy (FTIR)  Advanced technique.  Interferometer(Michelson Interferometer). 24 11/12/16Chetan Jain
  • 26. InterpretatIon of Ir Spectr 26 11/12/16Chetan Jain
  • 27. applIcatIonS of Ir and ft Ir  Qualitative application  Quantitative application. 27 11/12/16Chetan Jain
  • 28. References  Pharamceutical analysis by A.V. Kasture, S.G. Wadodkar,K.R. Mahadik, H.N. More, Volume II, Nirali Prakashan, Eleventh edition (2004), p.no. 207-221.  Fundamental of analytical chemistry by D.A.Skoog, D.M.West,F.J.Holler,7th edition p.no.592-597.  Organic Spectroscopy by William Kemp, 3rd edition, Palgrave Publication, p.no19-56.  Instrumental Methods of Analysis by H.A.Willard, L.L.Merritt, J.A.Dean, F.A. Settle, 7th edition, p.no. 288 to 320. 28 11/12/16Chetan Jain