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Bacteriological Culture Media
Culture media provide optimal
conditions
for growth & multiplication of bacteria.
BACTERIOLOGICAL LOOP
INOCULATION ON CULTURE PLATES
According to the physical state
media may be:
Fluid Media

Solid Media

Growth is visualized as

Turbidity

Colonies
(macroscopic products of 20-30 cell divisions of
a single bacterium on a solid medium)
Plating-out technique
 Procedure:
Loopful of the specimen

Specimen
Solid media are preferable:
 Isolation in pure culture.

 Identification
(colony morphology,
effect on medium).
Types of culture media:
1) Simple media
2) Enriched media
3) Selective media
4) Enrichment media
5) Indicator media
6) Selective and indicator media
1- SIMPLE MEDIA
1- SIMPLE MEDIA
a- Nutrient broth

Meat
Extract
SEA WEEDS
Sea weeds
AGAR POWDER
1- SIMPLE MEDIA
b- Nutrient agar

Nutrient Broth
+
2% agar-agar
1-SIMPLE MEDIA
Staphylococcus aureus on nutrient agar (golden yellow endopigment)
1- SIMPLE MEDIA
Ps. aeruginosa on nutrient agar (greenish exopigment)
1-SIMPLE MEDIA
Proteus on nutrient agar (swarming growth)
2-ENRICHED MEDIA
a- Blood agar ( also Indicator Medium)

Nutrient agar
+
5-10% Blood
2- ENRICHED MEDIA
Beta hemolysis on blood agar
2- ENRICHED MEDIA
Alpha hemolysis on blood agar
2- ENRICHED MEDIA
Non hemolytic organism on blood agar
2- ENRICHED MEDIA
Blood agar
2- ENRICHED MEDIA
b-Chocolate agar

Heated Blood agar
Haemoglobin
Heat
Haematin
3-SELECTIVE MEDIA
Lowenstein Jensen medium for M. tuberculosis

Beaten Eggs
+
Mineral salts
+
Malachite Green
4- ENRICHMENT MEDIA
Selenite F Broth
5- Differential (indicator) media
Triple Sugar Iron (TSI) agar

TSI agar
6- SELECTIVE INDICATOR (DIFFERENTIAL) MEDIA
MacConkey’s medium

Bile Salts
+
Lactose
+
Neutral Red
6- SELECTIVE INDICATOR (DIFFERENTIAL) MEDIA
MacConkey’s medium
Blood Culture

For isolation of bacteria from
blood samples in cases of
bacteraemia.
Blood culture technique

(2)
Added to
50 – 100 ml
fluid medium
(broth)

(1)
5 – 10 ml blood
(4)
Bacterial
Growth

(3)
Subculture on
Solid medium
Why use Large volume of broth???
1q111
1- Reduction of conc. of antibacterial
constituents in blood
2- Multiplication of bacteria
3- Neutralization of antibiotics
Anaerobic Cultivation

-For obligate anaerobes
Achieved by: - exclusion of oxygen
- addition of reducing substance
ANAEROBIC MEDIA
a- Robertson’s cooked meat medium
ANAEROBIC MEDIA
b- Thioglycollate Broth
GAS PAK SYSTEM FOR ANAEROBIC CULTIVATION
GAS PAK SYSTEM FOR ANAEROBIC CULTIVATION
MCQs
1) MacConkey’s medium:
a) Is an enriched medium.
b) Is an enrichment medium.
c) Contains phenol red as a pH indicator.
d) Gives pink colonies by lactose fermenting
bacteria.
e) Gives green colonies by lactose non-fermenting
bacteria.
2) Blood culture technique is used to
diagnose:
a) Sore throat
b) Conjunctivitis
c) Skin infection
d) Sinusitis
e) Bacteraemia
3) All are correct about enrichment media
except:
a)They are solid media.
b) Blood agar is not considered an enrichment
medium.
c)They have selective properties.
d)They help multiplication of certain species and
suppress others.
e)They include selenite broth.
4) All are correct about chocolate agar
EXCEPT:
a) It is an enrichment medium.
b) It contains heated blood.
c) It is used to grow fastidious organisms.
d) It is an enriched medium.
e) It is brown in colour.
5) One of the following is used to grow strict
anaerobes:
a) Selenite broth
b) MacConkey’s medium
c) Blood agar
d) Lowenstein-Jensen medium
e) Robertson’s cooked meat medium
6) When strict anaerobes are grown in the
Gaspak system:
a)They must be inoculated on cooked meat medium.
b)They can be grown on blood agar plates.
c) 50% carbon dioxide must be added.
d) The hydrogen released acts as a catalyst.
e)The palladium in the system acts as a nutrient.
Thank You For Your Attention

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