revision 2014

Scheme for slides
With background Without background
Brown Blue Red
Organism Gram +ve
cocci
Organism Gram -ve

Schemes for slides
With background Without background
Gram positive cocci Gram positive bacilli
Gram
negative
AND MIXTURES

S. pyogenes
Streptococci in pus

Quellung reaction
(Streptococcus pneumoniae)

Corynebacterium diphtheriae
(Gram stain)

(M.B. stain)

Bacillus anthracis
(Gram stain)

B. anthracis
McFadyean reaction(polychrome MB stain)

Mycobacterium tuberculosis
(Acid Fast bacilli Z-N stain)

Mycobacterium leprae (Acid Fast
bacilli with modified Z-N stain)

Mycobacterium leprae
(Acid Fast bacilli with modified Z-N
stain)

Gram negative bacilli & Staphylococci
Mixture

Spirochaetes (Dark-field microscopy)

Mixture: Candida & Gram –ve bacilli

C. albicans
(Positive Germ Tube test)

Staphylococcus aureus on nutrient agar
( showing golden yellow endopigment )

Pseudomonas aeruginosa on nutrient
agar(showing greenish exopigment)

Proteus on nutrient agar
(showing swarming growth)

Types of haemolysis on Blood agar

Alpha-haemolysis on blood agar

Bacitracin test
Streptococi other than
group A

Bacitracin test
Streptococci
other than group A
(Resistant)
group A streptococci
(Sensitive)

Optochin test
Sensitive
(Pneumococci)
Resistant
(Viridans streptococci)

Optochin test (sensitive)
Pneumococci

Optochin test (resistant)
Viridans streptococci

Corynebacterium diphtheriae on Blood
Tellurite

on Blood Tellurite

Loffler’s serum
for C. diphtheriae

Chocolate agar
for Neisseria and Haemophilus

Haemophilus or Neisseria on
chocolate agar

Haemophilus influenzae on blood agar showing
satellatism
(growth around staphylococcus culture)

Haemophilus influenzae
(growth only around both factors X & V)
xv

Lowenstein Jensen medium
for M. tuberculosis

Mycobacterium tuberculosis on
Lowenstein Jensen medium

M. tuberculosis on L.J. medium

Mycobacterium Growth Indicator Tube
(MGIT) (Positive tubes 1, 5 & 6)
1
2 3 4 5 6

Mycobacterium Growth Indicator
Tube (MGIT)
Positive
fluorescenceNegative
fluorescence

Lactose & Non-lactose fermenter on
MacConkey’s medium

Lactose fermenter on MacConkey’s
medium

Lactose fermenters on

Klebsiella (mucoid & LF) on

Lactose non-fermenter on MacConkey’s
medium

Lactose non-fermenter on

Salmonella on
Salmonella-Shigella (S-S) agar

Blood culture technique
1
2
35 – 10 ml blood
Added to
50 – 100 ml
fluid medium
Subculture on
Solid medium

Cooked meat medium
for anaerobic cultivation

Triple sugar iron( TSI )
TSI
Lactose
Fermenter + gas
e.g. E. coli,
Klebsiella
Glucose
Fermenter
e.g.Shigella,
Salmonella
Glucose
Fermenter
+ H2S
e.g.Salmonella
Non-fermenter
e.g.
Pseudomonas

TSI
Lf +gas
e.g.
E.coli,Kleb
NL
e.g.
Ps
TSI ggGl fer
e.g.Sal,
Shig
gl-ferm+H2S
e.g.
Salmonella

TSI
Non fermenter
e.g.
Pseudomonas
Lactose
ferm + gas
e.g. E. coli,
Klebsiella
Glucose
fermenter
e.g.Shigella,
Salmonella
Glucose
fermenter
+ H2S
e.g.Salmonella
Glucose
Fermenter
+H2S
e.g.Salmonella

Urease test
-ve +ve
e.g. Proteus

Catalase Test
e.g
Staphylococci
e.g.
Streptococci

Coagulase Test
Staphylococcus aureus
Coagulase negative
staphylococci,
e.g. S. epidermidis

Coagulase test
Staphylococcus aureus Coagulase negative staphylococci,
e.g. S. epidermidis

Oxidase test
Oxidase positive
e.g. Neisseria, Pseudomonas

Oxidase test
Oxidase positive
e.g. Neisseria,
Pseudomonas

Oxidase test
+ ve - ve
Oxidase positive
e.g. Neisseria,
Pseudomonas

Indole test
- ve
e.g. Klebsiella
+ ve
e.g. E.coli

MR & VP tests
MR test VP test
-ve +ve

B.R. of E. coli
I M V C + + - - TSI
MR VPIndole Citrate

B.R. of Klebsiella
I M V C - - + + TSI
VP

Nagler’s Reaction
(Clostridium perfringens)
Antitoxin No antitoxin

Nagler’s Reaction
(Clostridium perfringens)

Analytical Profile Index (API)
Biochemical identification system

String Test (Positive in Vibrio cholerae)

Tube agglutination test
(Titre:1/320)
TITRE
1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control

Brucella tube agglutination test
showing prozone phenomenon
(Titre:1/320)
TITRE
1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control

Widal Test (Recent infection with S. typhi)
(Titre:1/320)
O
H
C
T
C
PA
C
PB
C
1/20 1/40 1/80 1/160 1/320 1/640 1/1280
H
H

Widal Test (Old infection with S. typhi)
O
H
C
T
C
PA
C
PB
C
1/20 1/40 1/80 1/160 1/320 1/640 1/1280
H
H

Widal Test (Post vaccination)
O
H
C
T
C
PA
C
PB
C
1/20 1/40 1/80 1/160 1/320 1/640 1/1280
H
H

Latex Agglutination Test (A: Positive)

Latex agglutination Test (2 & 4: Positive )

Elek’s Test
Toxigenic C. diphtheriae Non-toxigenic C. diphtheriae

Tuberculin Skin Test (TST)
Positive

Antibiotic sensitivity (Disc diffusion)
1
2
3
6
5
4
The organism is
sensitive to 4, 5 & 6
Resistant to 1, 2 & 3

Antibiotic sensitivity (Disc diffusion)
Sensitive to…………
Resistant to………..

Phage-typing
1 2
3 4 5 6
7 8 9 10
11 12 13 14
15 16 17 18
19 20
The organism is sensitive to phages 4, 5, 6,……..etc.

Gel Electrophoresis
DNA
Marker
1 2 3 4 5 6

Western Blot
For diagnosis of HIV

Sterile cup
for collection of urine & sputum specimens

Sterile Gloves
For surgery & invasive procedures

Non- Sterile Gloves
For skin protection against blood & body fluids

Heavy-Duty Gloves
For environmental cleaning

Standard mask
Used for protection against droplet infections

N95 mask
Used for protection against airborne infections
e.g, M.tuberculosis

For protection against splashes of blood or body fluids
Aprons and Gowns

Eye Goggles
For protection against splashes of blood or body fluids

Rota virus (wheel-like appearance)

Coronavirus
(crown-like surface projection)

Tissue culture bottle
for cultivation of viruses

Choose and name the test used
to detect microbial antigen.
a
b
c d
a: Slide agglutination test

Choose & name the selective medium used for
isolation of Mycobacterium tuberculosis.
(A)
(B)
(C)
(D)
D: Lowenstein-Jensen medium

Infection control
The most important PPE
when dealing with patients
having diarrhoea is:
173
a.
c.
b.
d.
d: Non-sterile gloves

Final Practical Examination
NO Mobile Phones
are allowed in the department
(waiting rooms & exam. halls)

Practical exam. : Total 43 marks
Composed of 2 parts:
• Part 1: 13 marks
• Part 2: 30 marks

Part 1:
Demonstration:
Data show (total 13 Marks)
Formed of:
 3 different photos (3 marks/ 1 mark each)
ANY photo included in the modified revision CD
(version 2014)
 2 Choose a test
 2 Choose a medium
 1 Infection control
 5 Photos

Part 2:
Demonstrations (total 30 Marks)
Formed of 15 different stations (2 marks each)
In case of
a) microscopical slides: mention organism + stain
b) plain culture media: mention name + use
c) inoculated culture media: mention name +
the organism/effect
d) biochemical reactions: mention result + organism
e) serological test: mention name + result/titer
f) others: mention name + reading (e.g. phage-typing)

Name: Date:
No:
Microbiology Practical Exam
• 1)………………………………………………………………………………………………………………………..
• 2)………………………………………………………………………………………………………………………..
• 3)………………………………………………………………………………………………………………………..
• 4)………………………………………………………………………………………………………………………..
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• 6)………………………………………………………………………………………………………………………..
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• 12)………………………………………………………………………………………………………………………
• 13)………………………………………………………………………………………………………………………
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…………………………………………………………………………………………………………………………..
30 Marks (2 marks each)

revision 2014

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