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MYCOBACTERIUM Fungus Like Bacteria
[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],M.tuberculosis
Niacin test and nitrate reduction test -ve Niacin test &Nitrate reduction test &PO+ve no effect 0.5%glycerol-improves growth Dysgonic-sparsely Eugonic-grows luxuriantly microaerophilic primary isolation,aerobic on subculture obligate aerobe M.bovis M.tuberculosis
In liquid media-growth begins at bottom,creeps up the sides,forms prominent surface pellicle Flat smooth,white,moist,breakup easily when touched Solid medium-dry,rough,raised,irregular,wrinkled colonies-creamy white to yelow on incubation inc growth Sodium pyruvate-inc growth
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
ZN smear evln. & AFB report 4 + 1 F >= 10 3 + 1 F 1-9 2 + 10 F 1-9 1 + 100 F 1-9 Repeat smear 300 F 1-2 AFB not seen 300 F 0 report OIL imm.field No. of AFB
[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object]
Prophylaxis  ,[object Object],[object Object],[object Object]

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Mycobacterium

  • 2.
  • 3.
  • 4.
  • 5. Niacin test and nitrate reduction test -ve Niacin test &Nitrate reduction test &PO+ve no effect 0.5%glycerol-improves growth Dysgonic-sparsely Eugonic-grows luxuriantly microaerophilic primary isolation,aerobic on subculture obligate aerobe M.bovis M.tuberculosis
  • 6. In liquid media-growth begins at bottom,creeps up the sides,forms prominent surface pellicle Flat smooth,white,moist,breakup easily when touched Solid medium-dry,rough,raised,irregular,wrinkled colonies-creamy white to yelow on incubation inc growth Sodium pyruvate-inc growth
  • 7.
  • 8.
  • 9.
  • 10.
  • 11.
  • 12.  
  • 13.
  • 14.  
  • 15.
  • 16.
  • 17. ZN smear evln. & AFB report 4 + 1 F >= 10 3 + 1 F 1-9 2 + 10 F 1-9 1 + 100 F 1-9 Repeat smear 300 F 1-2 AFB not seen 300 F 0 report OIL imm.field No. of AFB
  • 18.
  • 19.
  • 20.
  • 21.
  • 22.
  • 23.
  • 24.