InVitria\'s Zap-CHO is a robust CHO cell culture media supplement designed to maximize CHO cell growth and productivity.

1. Zap-CHO Media Supplement Maximizes the Growth and Productivity of CHO Cells Steve Pettit, Ph.D Director of Cell Culture Development InVitria www.InVitria.com Info@Invitria.com (800) 916-8311

2. What is Zap-CHO? Zap-CHO is: A combination of defined recombinant proteins that maximize CHO growth and productivity Well defined and consistent from lot-to-lot Cost effective A FBS replacement for performance - without the animal source ZAP-CHO is NOT: A hydrolysate

3. Zap-CHO is Effective Across Media Types Relative Increase in Productivity 820 810 Protein Free Serum Free Chemically Defined Serum Free Protein Free Chemically Defined

4. Improved Growth Kinetics of CHO with Zap-CHO 10% FBS No Additive

5. Average Increase in Growth and Productivity *InVitria tests comparing the performance of Zap-CHO vs. hydrolysates and IGF1 show that Zap-CHO outperforms both of these supplements.

6. Zap-CHO Proteins are Removed by Protein A Reducing SDS-PAGE 75 KDa - Heavy Chain 50 Kda - Light Chain 25 Kda -

8. Higher peak cell density

9. Reduced apoptosis

10. Increased viability

11. Maximized productivity

13. Seed train expansion – Shorten time from cell bank to bioreactor

14. Cell adaptation– Shorten timelines for media adaptation

16. By Email: info@invitria.com

17. By Phone: 1-800-916-8311