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High Performance Liquid Chromatography FEROSEKHAN S   FNB-41 CIFE MUMBAI
Liquid Chromatography
Elution chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Outline ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
What is HPLC? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
HPLC is…. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
History ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Advantages to HPLC ,[object Object],[object Object],[object Object],[object Object],[object Object]
Advantages to HPLC ,[object Object],[object Object],[object Object]
Instrumentation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Schematic of liquid chromatograph
LC column LC injector
Partition Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Partition Chromatography  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Partition Chromatography  ,[object Object],[object Object],[object Object],[object Object],[object Object]
 
Adsorption Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object]
What is Ion Chromatography? ,[object Object],[object Object],[object Object],[object Object]
 
Size Exclusion Chromatography(SEC) ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],SEC(continued)
SEC Column Packing ,[object Object],[object Object],[object Object],[object Object]
 
The Mobile Phases are... ,[object Object],[object Object],[object Object],[object Object],[object Object]
Properties of the Mobile Phase   ,[object Object],[object Object],[object Object],[object Object]
Mobile phase reservoir ,[object Object],[object Object],[object Object],[object Object]
Elution methods ,[object Object],[object Object],[object Object],[object Object]
 
Sample Injection Systems ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Types of Detector ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Detectors ,[object Object],[object Object],[object Object],[object Object]
Refractive Index  ,[object Object]
Refractive Index ,[object Object],[object Object],[object Object],[object Object],[object Object]
Refractive Index ,[object Object],[object Object],[object Object],[object Object],[object Object]
Detectors ,[object Object],[object Object],[object Object]
UV/Visible  ,[object Object],[object Object]
UV/Visible ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Fluorescence ,[object Object],[object Object],[object Object]
Fluorescence ,[object Object],[object Object],[object Object],[object Object],[object Object]
Conductivity ,[object Object],[object Object],[object Object],[object Object]
Data System ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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