Poster – Development and automation of 3D innovative hiPSC-based liver organoids including the microenvironment for phenotypic screening – Application on metabolic diseases - version DHU2020

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Poster – Development and automation of 3D innovative hiPSC-based liver organoids including the microenvironment for phenotypic screening – Application on metabolic diseases - version DHU2020

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BIOMIMESYS® Adipose tissue represents a new generation of mimetic Hydroscaffold™ for 3D culture of adipocyte and adipocyte-like cells. Available in a ready-to-use format, it enables the culture of adipocytes and adipocyte-like cells under physiological conditions that are representative of the microenvironment found in adipose tissue. The highly porous nature of the scaffold allows the rapid uptake of nutrients, oxygen, etc. into the cells to create a reproducible study model for all downstream analyses used with 3D adipocyte cultures.

BIOMIMESYS® Adipose tissue

HCS Pharma

IOMIMESYS® Oncology is a new generation of mimetic Hydroscaffolds™ for 3D cell culture. Available in a ready-to-use format, it enables the culture of cells under physiological conditions that are representative of the microenvironment found in whole tissues. The highly porous structure of BIOMIMESYS® allows cells to diffuse into the 3D matrix, where they can fix and begin to develop. The structure and conformation of cells cultured in BIOMIMESYS®, such as cancer cells that spontaneously form spheroids, strongly resemble the structures formed in vivo. Also, the functionality of the BIOMIMESYS® matrix is proved by the expression of genes and proteins at levels that are again more similar to those found in vivo. Cells cultured in BIOMIMESYS® give results that are more physiological than those grown in 2D culture and are closer to those obtained in vivo.

BIOMIMESYS® Oncology

HCS Pharma

There is a 90% failure in clinical trials, due to efficacy and safety issues, which frequently concerns the central nervous system. That points a lack of relevance of preclinical models used upstream. In this frame, the aim of this study was to develop more relevant cerebral in vitro models, by including the matricial and cellular microenvironments, for drug discovery in Parkinson's disease. HD available on https://hcs-pharma.com/poster---importance-of-matricial-and-cellular-microenvironments-in-in-vitro-models-for-drug-discovery-in-parkinsons-disease/

Importance of matricial and cellular microenvironments in in vitro models for...

HCS Pharma

Growing interest in phenotypic screening, together with evidence that the drug response of cells grown in three-dimensional (3D) structures more closely resembles in vivo activity, has made high throughput, 3D fluorescence imaging an attractive screening option for drug discovery. However, creating 3D spheroids compatible with high content screening can be a difficult and expensive process. BIOMIMESYS® is a range of patented hyaluronic acid scaffolds for 3D cell culture. They are made of RGDS- and galactosaminegrafted hyaluronic acid, using an adipic acid dihydrazide crosslinker and extracellular matrix proteins (eg. type I, IV or VI collagen) to accurately mimic the in vivo extracellular environment. BIOMIMESYS® is suitable for automated testing thanks to the uniform thickness of the scaffold – around 650 μm – with an average porosity of 100 to 200 μm This application note describes a straightforward workflow for 3D cell seeding and spheroid formation using HCS Pharma’s BIOMIMESYS® plates in combination with INTEGRA’s VIAFLO 96/384 pipetting system. This process ensures rapid, controllable and reproducible 3D cell cultures, providing researchers with a highly efficient method to produce physiologically-relevant cellular models in a high throughput format

Simplifying 3d cell culture generation for high content screening with BIOMIM...

HCS Pharma

BIOMIMESYS® range are hyaluronan based hydroscaffold developed to overcome the 2D flat culture limitations by recreating an in vivo-like physiology within the in vitro environment. BIOMIMESYS®Liver hydroscaffold is made of RGDS and galactosamine-grafted Hyaluronic acid, Adipic acid dihydrazide crosslinker and extracellular matrix (ECM) proteins (collagen type I and collagen type IV) to mimic liver ECM composition.

BIOMIMESYS®Liver, a 3D cell culture model for maintaining and promoting hepat...

HCS Pharma

Abstract : a single procedure to generate functional hi ps cs-derived liver o...

HCS Pharma

Poster – Development and automation of 3D innovative hiPSC-based liver organo...

HCS Pharma

Development of a new liver-on-chip including BIOMIMESYS® technology for mimic...

HCS Pharma

HCS Pharma complète sa gamme de culture cellulaire en 3D BIOMIMESYS® avec BIO...

HCS Pharma

HCS Pharma extends its 3D cell culture range BIOMIMESYS® with BIOMIMESYS® Brain

HCS Pharma

A groundbreaking 3D cell culture technology for HCS: BIOMIMESYS hydroscaffold

HCS Pharma

We previously showed that human pluripotent stem cells (hiPSCs) provide a suitable model to study metabolic diseases upon hepatocyte-like cell (HLC) differentiation. In particular, HLCs have been used to model cholesterol metabolism regulation, by mimicking the main disease features in vitro. Human iPSCs can be generated from urine samples of patients with a well-described phenotype and carrying specific genotypes. This non-invasive approach allowed the study of LDLR- and PCSK9-mediated autosomal dominant hypercholesterolemia (ADH) as well as PCSK9-mediated familial hypobetalipoproteinemia (FHBL). While the direct link between hiPSCs and patients, as well as the abundance of HLCs provide promising advantages of such strategy, it is impaired mainly by the neonatal characteristic of HLCs as well as the difficulty to perform high throughput studies for pharmacological investigations. Therefore, to overcome these burdens, we choose to 1. Differentiate hiPSCs into HLCs in a 3D environment instead of the classical 2D culture systems to enhance their maturation; 2. Adapt our 3D differentiation process to a 96 wells format to make it compatible for drug screening. To reach our goals, we established a partnership with HCS Pharma, which has an expertise in high content phenotypic screening and produces an innovative 3D scaffold, BiomimesysTM. This scaffold is composed of hyaluronic acid that can be functionalized with extra cellular matric derivatives, with adjustable stiffness and porosity. We setup conditions for hiPSCs seeding and differentiation to reach a new protocol adapted to a 3D environment. Our preliminary data indicate that our procedure enhanced expression of hepatic markers such as transcription factors (FOXA2, FOXA3, HNF1a, HNF1b, HNF4a), cytochrome P450 (CYP450) family members (CYP3A4, CYP2A6, CYP7A1) or cholesterol metabolism regulators (PCSK9, Lipoprotein(a)). During our presentation, we will discuss our data hiPSCs differentiation in 3D, CYP450 activities and induction, as well as their application for the study of metabolic diseases.

3D innovative hiPSC-based models including the microenvironment for phenotyp...

HCS Pharma

As shown by AstraZeneca in Nature reviews*, one third of the safety failures is linked to CNS toxicity during the clinical trials of drugs. Therefore, relevant in vitro human model is needed to detect early neurotoxicity of drug candidates. In this study, sensitivity of iPSC-derived neuronal cells to 32 compounds is compared to the sensitivity of SH-SY5Y cells. Two types of iPS-derived cells are tested: central nervous system cells (CNS.4U™ cells) and peripheral nervous system cells (PERI.4U™ cells) from Ncardia. Toxic effects are then measured by HCS cell imaging.

Neurotoxicity assessment: Comparison between SH-SY5Y and iPSC-derived cells

HCS Pharma

BIOMIMESYS® range are hyaluronan based scaffolds developed to overcome the 2D flat culture limitations by recreating an in vivo physiology within the in vitro environment. BIOMIMESYS® Adipocyte scaffold is made of RGDS-grafted Hyaluronic acid (1.6 MDa), Adipic acid dihydrazide crosslinker and extracellular matrix (ECM) proteins (collagen type I and collagen type VI) to mimic fat tissue-ECM composition.

BIOMIMESYS® Adipose tissue, a relevant in vitro adipocyte 3D model

HCS Pharma

BIOMIMESYS® range are hyaluronan based hydroscaffold developed to overcome the 2D flat culture limitations by recreating an in vivo-like physiology within the in vitro environment. BIOMIMESYS®Liver scaffold is made of RGDS and galactosamine-grafted Hyaluronic acid, Adipic acid dihydrazide crosslinker and extracellular matrix (ECM) proteins (collagen type I and collagen type IV) to mimic liver-ECM composition.

BIOMIMESYS® Liver, a 3D cell culture model for maintaining and promoting hep...

HCS Pharma

BIOMIMESYS® Biofunctionalized hydroscaffold for 3D cell culture

HCS Pharma

HCS for brain disorders / HCS Pharma at B4B mars 2018

HCS Pharma

La société HCS Pharma, spécialiste de l’imagerie cellulaire à haut débit et du développement de nouveaux modèles cellulaires, est née à Rennes (35) en août 2014. Le 6 octobre dernier, elle a inauguré de nouvelles installations à Lille (59), au cœur du parc Eurasanté, et y a mis à l’honneur notamment sa toute nouvelle plate-forme robotique baptisée HAPIx, HCS Automation Platform for Imaging. Nathalie MAUBON, Présidente de l’entreprise, nous présente HCS Pharma et ses nouvelles ambitions portées par cette implantation lilloise, au plus proche des chercheurs et cliniciens en santé humaine...

Présentation de HCS Pharma dans la gazette du laboratoire - 2017

HCS Pharma

Human induced pluripotent stem cells (hiPSC) are becoming a relevant model for the study of liver metabolic diseases once differentiated into hepatocyte-like cells (HLC), and it has been shown that they can faithfully recapitulate autosomal dominant hypercholesterolemia (ADH). PCSK9 is a critical modulator of cholesterol homeostasis, and quickly became a hot target for ADH pharmacological treatment strategies. However, current cellular models to further decipher the role of PCSK9 in ADH are limited, especially to study the PCSK9 gain of function mutation S127R, which seems to interfere with LDL cholesterol homeostasis intracellularly by still unknown mechanisms.

Urine sample-derived human induced pluripotent stem cells as a model to study...

HCS Pharma

It was a real pleasure to be in the « High-Content and Phenotypic Screening » meeting in Cambridge. We were invited by our partner Molecular Devices to give a talk during the "User meeting Molecular Devices" about our vision of 3D culture in phenotypic screening and the impact of new technologies. We also presented a poster about "Neurotoxicity assay on 2D and 3D culture using High Content Screening technology".

3D culture in phenotypic screening : advantages, process changes and new tech...

HCS Pharma

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