Shared Industrial-scale Low-density SNP Genotyping for CGIAR and Partner Breeding

1. Rajaguru Bohar
HTPG – Project Coordinator
R.Bohar@cgiar.org ; HTPG@cgiar.org
HTPG (2016-2020) - Project Updates
RP Genetic Gains meeting with DDG- R
Jan 5-6, 2021
Shared Industrial-scale Low-density SNP
Genotyping for CGIAR and Partner Breeding
Programs Serving SSA and SA

2. Low Density (HTPG) Platform
• Shared genotyping platform serving all CGIAR centers
and their partner programs
• Low Density KASP based SNPs Genotyping(1-200 SNPs)
• $2-$2.5 per sample(10 SNPs Genotyping, DNA extraction & QC)
• Trait (100+) & QC genotyping – 1500+ SNPs
• Centers under the agreement:
• 38+ CGIAR and NARs users to date (28 countries),
• Private partners,
• USDA, Embrapa, CIRAD, CSIRO,….
• Target Crops:
• Wheat, Maize, Rice, Groundnut, Chickpea, Pearl Millet, Potato, Cassava,
Sorghum, Cowpea, Common Bean, Soybean, Pigeon Pea, Finger Millet, Yam,
Banana, Tilapia,...
• Tomato, Cocoa, Coffee (soon)

3. Capacity Development and Training

4. Crop wise data points
Updated as on: 2016-Q3 2020

5. Data Point and Business Volume - Summary
Total Business Volume: Surpassed $2,737,224
(Circa. $1 Mio from the project)
HTPG Data Points
Total Data Points: Surpassed 11,244,352
Updated as on: 2016-Q3 2020

6. INDIA - NARS
Indira Gandhi Krishi Vishwavidyalaya (IGKV), RAIPUR
Regional Agricultural Research Station (RARS),MARUTERU
Agricultural Research Station (ARS) - Bapatla
ICAR- National Rice Research Institute (NRRI), Cuttack
Assam Agricultural University (AAU), Jorhat
ICAR- Indian Institute of Rice Research (IIRR), Hyderabad
The Indian Agricultural Research Institute, New Delhi
Professor Jayashankar Telangana State Agricultural University
(PJTSAU), Telangana
IRRI -
RICE
HTPG
Bangladesh - NARS
Bangladesh Rice Research
Institute (BRRI)
Philippines - NARS
PhilRice
INDIA - NARS
ICAR-Indian Institute of Wheat & Barley Research, Karnal
The Indian Agricultural Research Institute, New Delhi
University Agricultural Science, Dharwad
Nepal - NARS
Agriculture and Forestry University, Rampur, Chitwan
CIMMYT -
Wheat
CIP -
Potato
INDIA - NARS
ICAR-Central Potato Research Institute, ShimlaPublic - Private - Partnership
Mahindra Seeds (Hyderabad)
circa 1.0 Million
Data pointsICRISAT
Crops
INDIA >13 NARS/SAU/ARS
Asia CG / NARS Partnership

7. Opportunities Challenges
 Implementing common SOP
 Different breeding programs/
partners at different stage
 Genotyping Core Breeding budget
 Resource limitation especially for
NARS & SAU
 Requires robust training
 Backstopping & follow up
 Diverse crop/region
 Each crop requires unique strategies
 Ploidy etc.
 Logistics

8. Our Team & Contributors

9. Acknowledgments