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Cytotoxicity of silicone materials used in maxillofacial prosthesis / dental implant courses by Indian dental academy 


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Cytotoxicity of silicone materials used in maxillofacial prosthesis / dental implant courses by Indian dental academy 

  1. 1. Cytotoxicity ofCytotoxicity of Commercially AvailableCommercially Available Silicone Material UsedSilicone Material Used for Maxillofacialfor Maxillofacial ProsthesisProsthesis INDIAN DENTAL ACADEMY Leader in continuing Dental Education
  2. 2. IntroductionIntroduction
  3. 3. Purpose of the StudyPurpose of the Study  The purpose of this study was to qualitatively assess the potential cytotoxicity of various (Biomed and Realistic) commercially available silicon materials used vastly for maxillofacial prosthesis in prosthetic dentistry.
  4. 4. Materials & MethodMaterials & Method A metallic die with diameter -28mm and depth - 2mm was fabricated to standardize the samples. Realistic and Biomed silicone materials. Two commercially available materials Biomed and Realistic were used to evaluate cytotoxicity.
  5. 5. Realistic groupBiomed group Five samples for each group were fabricated.
  6. 6. Pressure gauge indicating the pressure applied Autoclave unit All the samples of both groups were sterilized in autoclave at 121°C at 10 pounds of pressure for 30 minutes. The autoclave has an outer jet and an inner jet. First the pressure is created in the outer jet and then transferred in the inner jet.
  7. 7. Cells and MediumCells and Medium Syrian hamster; kidney cells in fetal bovine serum Eagle’s Minimum Essential Medium (MEM) MEM is a complex media which contains a large number of amino acids and vitamins and is often supplemented with extra metabolites and minerals
  8. 8. Selection of medium for serumSelection of medium for serum  There are few good guidelines for selection of appropriate medium for a given cell type, but it is based on:- 1) Literature or source of cells. 2) Otherwise choice is either empirical or by comparative testing of several media.
  9. 9. Cells and MediumCells and Medium  Syrian hamster; kidney cells were used. They were grown in Eagle’s Minimum Essential Medium (MEM) with 2mM L-glutamine and Earle’s BSS adjusted to contain 1.5g/L Na bicarbonate, 0.1mM non-essential amino acids, and 1.0mM Na pyruvate, 90% fetal calf serum.
  10. 10. Procedure to measure cellProcedure to measure cell cytotoxicitycytotoxicity  These samples were divided into three groups: 1. Control group. 2. Realistic group. 3. Biomed group.
  11. 11.  Control group – In this only the MEM (Eagle’s medium) and hamster kidney cells were taken Petri dish showing the control group
  12. 12. Biomed group Realistic group.  The silicon material samples were placed in the culture media. Then the media was removed by aspiration and placed in the vials. The cells were transferred to the vials.
  13. 13.  The LAMINAR FLOWHOOD works on a function of HEPA (High efficiency particulate air) to make the air sterile.  The microbiologist is working under LAMINAR FLOWHOOD which is a sterilized chamber to maintain the non microbial environment.  Here the microbiologist is transferring the Syrian hamster; kidney cell line to the culture media.
  14. 14.  Incubation vials with the samples after transferring the cell lines
  15. 15. Incubator with the vials  Samples were innoculated with cell lines and were incubated for 3 days at 37°C and 5% carbon dioxide atmosphere.
  16. 16.  The optimal temperature for the incubation is dependent on three factors:- 1) Body temperature of the animal from which the cells are obtained. 2) Any regional variation eg. cells from the skin. 3) Incorporation of safety factor to allow minor errors in incubator regulation.
  17. 17.  Cytotoxicity was observed by recording any change in the morphologies of the syrian hamster kidney cells (with the help of the OLYMPUS phase contrast microscope) as compared with the controls and the results from these 3 groups were recorded and scored for each sample on a 0 - 4+T scale:  0  no cytotoxicity  1+T  1-25% effect  2+T  25%-50% effect  3+T  50%-75% effect on cells  4+T  75%-100% destruction of the cell
  18. 18.  OLYMPUS phase contrast microscope
  19. 19. ObservationsObservations  Whirlpool pattern arrangement of syrian kidney cells can be seen in the control group under the phase contrast microscope appearance Without filter With
  20. 20. Micrographic view of Biomed group after incubation shows morphological damage of culture cells
  21. 21. Micrographic view of Realistic group after incubation shows morphological damage of culture cells
  22. 22. ResultsResults GROUPGROUP CYTOTOXICITYCYTOTOXICITY ControlControl 00 RealisticRealistic 11 BiomedBiomed 11
  23. 23. Clinical ImplicationsClinical Implications  The probability of allergic or toxic reactions from silicon materials or their components is small but the following test results show that the potential exists.  The perceptive practitioner will be alert to the subtle signs of tissue response that may manifest itself with the use of silicon materials.
  24. 24. DiscussionDiscussion DiscussionDiscussion
  25. 25. LimitationsLimitations  The sample size was less.  It was the qualitative not the quantitative test.  Only one method to evaluate the cytotoxicity was done.  Other methods to evaluate cytotoxicity such as cell viability, cell culture agarose overlay tests can be done to correlate the results.
  26. 26.