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Photosynthesis
Autotrophs vs. Heterotrophs 
Autotroph 
 Make their own 
food 
Example: Plants 
Heterotroph 
 Obtain energy from 
foods they eat 
Example: Animals, 
fungi
Autotrophs 
Photosynthesis occurs within: 
Some bacteria 
Algae 
Within leaves of plants 
does NOT happen in ALL cells
Chemical Energy 
Energy – the ability to do work 
Adenosine Triphosphate (ATP) 
Adenine 
Ribose 
3 phosphate groups 
ADP + P 
Adenosine diphosphate + phosphate
Chemical Energy 
= 
Adenosine 
Triphosphate 
(ATP)
Photosynthesis 
The process of creating sugar 
from CO2. 
Consumes H2O and is powered 
by light.
Photosynthesis Equation 
Light 
CO2 + H2O  C6H12O6 + O2 
carbon dioxide + water  sugars + oxygen 
6 6 6
Electromagnetic Spectrum
Absorption of Light by 
Chlorophyll a and Chlorophyll b
Light and Pigment 
Plants gather the Sun’s energy 
with light absorbing molecules 
called pigments 
Chlorophyll (Green) 
chlorophyll a and chlorophyll b
Chloroplast 
Stroma - Liquid 
Granum – stack 
of thylakoids 
Thylakoid – contains chlorophyll
Photo Synthesis 
Light To make or create
Photosynthesis Part I: 
Light Dependent Reactions 
Location: Thylakoid Membrane
Light-Dependent Reactions
Light-Dependent Reactions 
Location: Thylakoid Membrane 
1. Photosystem II 
 Light energy absorbed by electrons 
and increases their energy level 
 Enzymes break up water molecules 
into: 
 2 electrons 
 2 hydrogen ions 
 1 oxygen atom
Light-Dependent Reactions 
Location: Thylakoid Membrane 
2. Electron Transport Chain 
 High-energy electrons move 
hydrogen ions from the stroma 
into the inner thylakoid space
Light-Dependent Reactions 
Location: Thylakoid Membrane 
3. Photosystem I 
Uses energy from light to re-energize 
the electrons. 
NADP+ picks up electron and 2 H+ 
ions 
Produces NADPH
Light-Dependent Reactions 
Location: Thylakoid Membrane 
4. Hydrogen Ion Movement 
 Hydrogen ions move 
across the thylakoid 
membrane making the 
inside positively charged
Light-Dependent Reactions 
Location: Moves from Thylakoid to Stroma 
5. ATP Formation 
Hydrogen ions pass through ATP 
synthase (protein) 
Energy is converted from ADP 
to ATP
Photosynthesis Part II: 
Light Independent Reactions 
a.k.a. Calvin Cycle 
Location: Stroma
Calvin Cycle 
Uses CO2, ATP and 
NADPH to produce 
high energy sugars
Calvin Cycle 
Location: Stroma 
1. Carbon Fixation 
6 CO2 combines with a 
five-carbon sugar 
Enzyme breaks down to 
12 three-carbon molecules
Calvin Cycle 
Location: Stroma 
2. Energy Input 
ATP is broken down giving each 
three-carbon molecule a 
phosphate 
NADPH gives up electrons to 
provide more energy
Calvin Cycle 
Location: Stroma 
3. Sugar Production 
2 three-carbon molecules exit 
cycle to be used by the plant 
10 three-carbon molecules are 
recycled
Calvin Cycle 
Location: Stroma 
4.5-Carbon Molecule Regeneration 
10 three-carbon molecules are 
converted back into 6 5-carbon 
molecules to be used in the next 
cycle 
6 ATP molecules are used to complete 
this process
Factors Affecting Photosynthesis 
Water 
Plants in dry conditions have a waxy coating 
on the leaves that reduce water loss 
 Temperature 
Photosynthesis depend on enzymes that 
function best between 0oC and 35oC 
 Light Intensity 
Increasing light intensity increases the rate 
of photosynthesis 
However, a plant has a maximum rate of 
photosynthesis that can be reached

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Photosynthesis Notes

  • 2. Autotrophs vs. Heterotrophs Autotroph  Make their own food Example: Plants Heterotroph  Obtain energy from foods they eat Example: Animals, fungi
  • 3. Autotrophs Photosynthesis occurs within: Some bacteria Algae Within leaves of plants does NOT happen in ALL cells
  • 4. Chemical Energy Energy – the ability to do work Adenosine Triphosphate (ATP) Adenine Ribose 3 phosphate groups ADP + P Adenosine diphosphate + phosphate
  • 5. Chemical Energy = Adenosine Triphosphate (ATP)
  • 6. Photosynthesis The process of creating sugar from CO2. Consumes H2O and is powered by light.
  • 7. Photosynthesis Equation Light CO2 + H2O  C6H12O6 + O2 carbon dioxide + water  sugars + oxygen 6 6 6
  • 9. Absorption of Light by Chlorophyll a and Chlorophyll b
  • 10. Light and Pigment Plants gather the Sun’s energy with light absorbing molecules called pigments Chlorophyll (Green) chlorophyll a and chlorophyll b
  • 11. Chloroplast Stroma - Liquid Granum – stack of thylakoids Thylakoid – contains chlorophyll
  • 12. Photo Synthesis Light To make or create
  • 13. Photosynthesis Part I: Light Dependent Reactions Location: Thylakoid Membrane
  • 15. Light-Dependent Reactions Location: Thylakoid Membrane 1. Photosystem II  Light energy absorbed by electrons and increases their energy level  Enzymes break up water molecules into:  2 electrons  2 hydrogen ions  1 oxygen atom
  • 16. Light-Dependent Reactions Location: Thylakoid Membrane 2. Electron Transport Chain  High-energy electrons move hydrogen ions from the stroma into the inner thylakoid space
  • 17. Light-Dependent Reactions Location: Thylakoid Membrane 3. Photosystem I Uses energy from light to re-energize the electrons. NADP+ picks up electron and 2 H+ ions Produces NADPH
  • 18. Light-Dependent Reactions Location: Thylakoid Membrane 4. Hydrogen Ion Movement  Hydrogen ions move across the thylakoid membrane making the inside positively charged
  • 19. Light-Dependent Reactions Location: Moves from Thylakoid to Stroma 5. ATP Formation Hydrogen ions pass through ATP synthase (protein) Energy is converted from ADP to ATP
  • 20.
  • 21. Photosynthesis Part II: Light Independent Reactions a.k.a. Calvin Cycle Location: Stroma
  • 22. Calvin Cycle Uses CO2, ATP and NADPH to produce high energy sugars
  • 23. Calvin Cycle Location: Stroma 1. Carbon Fixation 6 CO2 combines with a five-carbon sugar Enzyme breaks down to 12 three-carbon molecules
  • 24. Calvin Cycle Location: Stroma 2. Energy Input ATP is broken down giving each three-carbon molecule a phosphate NADPH gives up electrons to provide more energy
  • 25. Calvin Cycle Location: Stroma 3. Sugar Production 2 three-carbon molecules exit cycle to be used by the plant 10 three-carbon molecules are recycled
  • 26. Calvin Cycle Location: Stroma 4.5-Carbon Molecule Regeneration 10 three-carbon molecules are converted back into 6 5-carbon molecules to be used in the next cycle 6 ATP molecules are used to complete this process
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  • 29. Factors Affecting Photosynthesis Water Plants in dry conditions have a waxy coating on the leaves that reduce water loss  Temperature Photosynthesis depend on enzymes that function best between 0oC and 35oC  Light Intensity Increasing light intensity increases the rate of photosynthesis However, a plant has a maximum rate of photosynthesis that can be reached