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Diffusion Tensor Imaging Analysis-3749
1. Diffusion Tensor Imaging Sonia Pujol, Ph.D. Randy Gollub, M.D., Ph.D. National Alliance for Medical Image Computing
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4. Goal of this tutorial Guiding you step-by-step through the DWI data analysis capabilities of Slicer, including generation of tensors, calculation of scalar metrics and tractography tools. Final result of the tutorial
8. Diffusion Weighted Imaging (DWI) Example: Correlation between the orientation of the 11 th gradient and the signal intensity in the Splenium of the Corpus Callosum
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12. Loading the DWI Training Dataset 1 Click on Add Volume to load the Dicom-DWI training dataset
13. Loading DWI data Select Nrrd Reader in the Properties field The Props Panel of the module Volumes appears.
14. Loading DWI data Click on Apply Click on Browse and load the file namic01-dwi.nhdr in the directory Dwi-dicom Check that the path to the file is correct. If needed, manually enter it
19. Loading DWI data The sagittal and coronal viewers display the 14 MR volumes: 2 baselines and 12 diffusion weighted volumes
20. Loading DWI data Left-Click on the V button to display the axial and sagittal slices inside the viewer. Use the axial slider to slice trough the baselines and diffusion weighted MR volumes.
21. DT-MRI Module Select Modules in the Main Menu Select Visualisation DTMRI
22. DT-MRI Module The panel Input of the DTMRI module appears Click on the tab Conv
23. DT-MRI Module The panel Conv of the DTMRI module appears
24. Converting DWI data to tensors Select the Input Volume namic01-dwi.nhdr and click on ConvertVolume
25. Converting DWI data to tensors (Stejskal and Tanner 1965, Basser 1994 ) {Si} represent the signal intensities in presence of the gradients gi Slicer computes the symmetric 3x3 tensor matrix D for each voxel zz zy zx yz yy yx xz xy xx D D D D D D D D D
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27. Converting DWI data to tensors Slicer displays the anatomical views of the average of all 12 diffusion weighted images (average over all gradient directions)
29. Adjusting Window Level Select the Active Volume namic01-dwi-nhdr_AvGradient Use the sliders Win and Lev to adjust the Window level
30. Adjusting Window Level Slicer displays the anatomical views of the average of all 12 diffusion weighted images (average over all gradient directions) Inspect the anatomy using the axial, sagittal and coronal sliders.
31. Converting DWI data to tensors Left-click on Bg and select the volume namic01-dwi nhdr _Baseline
32. Converting DWI data to tensors Browse the baseline images to check if the anatomy is correct Slicer displays the baseline (T2) images.
33. Converting DWI data to tensors Click on the module Data Slicer displays the list of DTI volumes
34. Converting DWI data to tensors Select File Close in the Main Menu to clear the scene
36. Loading Slicer Sample DWI data Select ImageHeaders: Auto Click Apply Select the Props Panel Use the Basic Reader Click on Browse Navigate to the folder containing the tutorial data Select the first file D.001 Click Open
39. Loading Slicer Sample DWI data A sequence of white stripes appears in the diffusion weighted images. They correspond to intersections with the baseline images in Slicer Axial/Sagittal/Coronal (AxiSagCor) slice mode.
40. Loading Slicer Sample DWI data Inferior Superior In Axial/Sagittal/Coronal mode the slices planes, which are aligned with the RAS coordinates, are cutting through the DWI volume
41. Loading Slicer Sample DWI data Left click on Or and select the orientation Slices in the Menu
49. Loading Slicer Sample DWI data Notice that the image intensity for each of the six gradient orientations is much lower than the S 0 image.
50. DT-MRI Module Select Modules in the Main Menu Select Visualization DTMRI
51. DT-MRI Module Select the Conversion Panel: Conv Select the module DTMRI
52. Convert DWI data to tensors Select InputVolume D Select Protocol BWH_6g.1bSlice Click on Prop to display the parameters of the acquisition protocol
60. Computing Fractional Anisotropy Select the panel Scalars Browse the menu Create Volume to see the list of calculations that Slicer can perform on the D_Tensor dataset. Select Fractional Anisotropy
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62. Computing Fractional Anisotropy Click on Apply Select the Region of Interest ROI:Mask The Scale Factor is set by default to 1000, because the standard range of FA values (0.0 to 1.0) is not compatible with Slicer The Fractional Anisotropy Panel appears
63. Computing Fractional Anisotropy The Viewer displays the FA volume. Move the mouse in the slices to see FA values for each voxel.
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66. ROI Drawing Select the Editor module in the main Menu. Select the Volumes panel. Select the Original Grayscale FractionalAnisotropy_D Select the Working Labelmap NEW and keep the Default Descriptive Name Working. Click on Start Editing
67. ROI Drawing Select the Effects panel Left click on Draw in the Effects Menu
68. ROI Drawing The Draw Panel of the Editor Module appears Left-click on Output, and select the color label #2 (pink)
69. ROI Drawing Draw the contour of the Corpus Callosum with the mouse in the sagittal slice
71. Measure FA Statistics in ROI Select Modules Measurement VolumeMath in the Main Menu
72. MaskStat Select MaskStat The MaskStat functionality uses the labelmap as a mask over the FA volume, and calculates “ stats ” on the region contained under the labelmap.
73. MaskStat Set Volume to Mask to FractionalAnisotropy_D_Tensor Set LabelMap to Working Set Masked Output to Create New
74. MaskStat Click on Run Click on Mask , select the same color as your labelmap Click on Browse to select a directory to place the output text file and enter the file name FractionalAnisotropy_D_Tensor_hist.txt
75. MaskStat Result A window shows the statistics (multiplied by the Scale Factor): minimal, maximal, mean and standard deviation of the FA values. The results have been saved in the file FractionalAnisotropy_D_Tensor_hist.txt written on the disk.
76. Save FA volume and ROI Select the module Editor Select the panel Volumes and click on Save Enter a FilenamePrefix and select the format NRRD(.nhdr) Click on Save
77. Save FA volume and ROI Slicer generates a Nrrd header file (FA.nhdr), and a raw compressed file (FA.raw.gz). Save the Working volume containing the label map using the same process. (Slicer Training #7: Saving data.)
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79. Tractography Panel Select the DTMRI module and click on the Panel More Select the Panel Tracts inside the DTMRI module
81. Tractography Panel A Color selection panel appears Select a new color for the tracts
82. Create a single tract Position the mouse on a point inside the Corpus Callosum, and hit the s key.
83. Create a single tract A tract appears in the 3D Viewer. Drag right mouse button down in the 3D Viewer to zoom in.
84. Create a single tract Click on the V buttons The 3D window shows a closer view of the tract.
85. Create a single tract Slicer displays the slices in the 3D window. Drag left mouse button in the 3D Viewer to rotate the volume, Drag right mouse button to zoom in, until you get to a convenient view.
86. Generate Multiple Tracts Position the mouse on different points in the corpus callosum and hit the s key.
87. Generate Multiple Tracts The tracts that correspond to the visited points appear in the 3D Viewer.
91. ROI Drawing Select the Editor module in the main Menu. Select the Volumes panel and click Setup Select the Original Grayscale FractionalAnisotropy_D_Tensor Select the Labelmap Working. Click on Start Editing
92. ROI Drawing Select the Effects panel Left click on Draw in the Effects Menu
94. ROI1: Temporal stem Draw a region of interest in the Temporal stem (slice #156) Select View 1x512 COR in the Main Menu Click on Apply in the module Editor
96. ROI2: Posterior temporal lobe Draw a region of interest in the Posterior Temporal Lobe (slice #128) Select View 1x512 COR in the Main Menu. Click on Apply in the module Editor
98. ROI3: Splenium Draw a region of interest in the Splenium of the Corpus Callosum (slice #131) Select View 1x512 SAG in the Main Menu. Click on Apply in the module Editor.
99. ROI Seeding Come back to the DTMRI module and select the panel Tracts . Click on the tab Seed and select the SeedROI Working Select the color label #7 corresponding to the ROI1
100. ROI Seeding Click on Seed Tracts A warning message appears, Click Yes if you are ready to process the data.
102. ROI Seeding Select the color label #10 corresponding to the ROI2 Click on Seed Tracts A warning message appears, Click Yes if you are ready to process the data.
104. ROI Seeding Select the color label #5 corresponding to the ROI3 Click on Seed Tracts A warning message appears, Click Yes if you are ready to process the data.
111. Selective Seeding Click on the tab Select in the Panel Tract Select the ROI Labelmap Working Enter the labels of the ROI1 (label #7) and ROI2 (label #10) in the list of labels called AND .
112. Selective Seeding Click on Find Tracts through ‘ROI’ Enter the label of the ROI3 (label #5) in the list of labels called NOT.
113. Selective Seeding Slicer displays the resulting tracts of the Inferior Occipito-frontal Fasciculus in red.
119. ROI Seeding Select the ROI Working Click on Seed Tracts Select the color label of the ROI (#2) A warning message appears, Click Yes if you are ready to process the data.
125. Clustering Method* Cluster-colored tracts (*) White Matter Tract Clustering and Correspondence in Populations O’Donnel L, Westin C-F Medical Image Computing and Computer-Assisted Interventions (MICCAI2005)
126. Tract Clustering Algorithm Click on More and select the panel TC In this tutorial example, we cluster the fiber tracts generated from the ROI in the Corpus Callosum