1. PLANT TISSUE CULTURE
PRESENTED BY: INSTITUTE OF
LOK HITH KUMAR PAHARAMCEUTICAL
B.PHARMA RESEARCH
FINAL YEAR. GLA UNIVERSIYTY.
2. Introduction
• Tissue culture is a special type of asexual
propagation where a very small piece of
tissue (shoot apex or even individual cell)
is placed in sterile culture in a test tube.
• It was Ist done in1885 by Wilhelm roux he
extracted small part of medullary
plate embryo of chicken.
3. Defination of PTC
• PTC is a collection of technique used to
maintain or grow plant cells, tissue or organ,
Under sterile condition on a nutrient culture
medium of known composition.
4. Principles
• Selection of explants.
• Sterilization of explant.
• Plant transferred to solid culture
media.
• Addition of hormone.
• Placed in another media.
• Transplantation.
5. Selection of explant
• The explant is selected
it is either haploid or
diploid explant
• The plant growth can be
achieved in two ways:
1.shoots directly by
appropriate media
2.By somatic
embryogenesis
6. Sterilization
• Sterilization of
explant placed in
solid media is
done by mercuric
chloride.
7. Culturing
• Culturing media
contains gelling
1) Essential elements or
agents
mineral ions, supplied as a
complex mixture in liquid media with
of salts. proper mixture of
(2) An organic supplement nutrients sugar,
supplying vitamins and vitamins and
amino acids, and
hormones.
(3)A source of fixed carbon,
usually supplied as the sugar
sucrose.
8. Hormone for Ptc
• Auxin and cytokinin are
require for plant
growth.
• Excess of auxin results
in proliferation of roots.
• Excess of cytokinin
results into proliferation
of shoots.
9. Multipilcation media
• It make a big blob of
tissue called callus.
• It may directly lead to
formation of new
shoots from explant.
• The callus start‘s
formation of new
plantlets.
10. Removing into new culture
• The growing plant is
transferred to new
culture media so it
can multiply their &
produce more shoot
with out deprivation
of nutreints.
11. Transplantation
• When plantlets
become larger they
are transfered to
the pots.
• The young plants
are grown in green
house.
12. Applications
• Can conserve
endangered species.
• Can obtain viral free
plant.
• Hybrids can be
regenerated by
protoplasm fusion.
• Plant can be grown
in less time.
13. Difference in PTC& Animal culture
• It grow on at specific temperature i.e. normal
temperature for human is 37°c.
• Carbon dioxide is also require.
• It needed proper change in media otherwise
cells will not grow properly.
• Animal cells needed protein and hormones for
proper development.
14. Advantage
• Exact copy of the plant
is obtained.
• Production of multiple
plant without pollinator.
• Whole plant can be
genetically modified.
• Totipotency can be used
i.e. whole plant can
grown by single cell.
