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Developmental Biology: From Embryology to Molecular Techniques
- 2. Developmental Biology vs Embryology Embryology – observational biology – experimental manipulations Developmental Biol – expands embryological studies using molecular techniques – used genetic approaches to study model systems
- 3. Developmental Biology Approaches in Other Fields Cancer Biology Neuroscience The answers to questions in these fields require knowing when during development particular events occur
- 4. Developmental Biology is Defined by the Questions Asked Differentiation Morphogenesis Growth Reproduction Evolution Environmental Integration
- 5. What goes on as an embryo develops from a single, pluripotent cell into a complete organism? Majority of dev systems are multicellular Single-celled systems used to study cellular differentiation Differentiation Morphogenesis Growth Reproduction Evolution Environmental Integration Developmental Biology is Defined by the Questions Asked
- 6. Cell movements & tissue reorganizations How do differentiated cells move and reorganize during development to generate functional organisms? Major point of study for classical embryology Developmental Biology is Defined by the Questions Asked Differentiation Morphogenesis Growth Reproduction Evolution Environmental Integration
- 7. What are the limiting factors for cell division Allometric growth - varying rates of mitosis in regions of the embryo Isometric growth- equal rates of mitosis throughout embryo Developmental Biology is Defined by the Questions Asked Differentiation Morphogenesis Growth Reproduction Evolution Environmental Integration
- 8. The germ plasm Gametogenesis - formation and maintenance of pluripotent, haploid germ cells (gametes) Recognition & interaction of gametes at fertilization Developmental Biology is Defined by the Questions Asked Differentiation Morphogenesis Growth Reproduction Evolution Environmental Integration
- 9. Survivability of evolutionary mutations is limited by restraints of embryogenesis Evolutionary changes must be small changes for the embryo What are the evolutionary relationships between groups of organisms Developmental Biology is Defined by the Questions Asked Differentiation Morphogenesis Growth Reproduction Evolution Environmental Integration
- 10. Early development of many organisms is influenced by environmental cues Examples: color/shape of butterfly larvae (caterpillars) diapause in invertebrates in vernal pools Developmental Biology is Defined by the Questions Asked Differentiation Morphogenesis Growth Reproduction Evolution Environmental Integration
- 11. Aristotle (300BC) – credited with 1st notions on embryology William Harvey (mid 1600s) – hypothesized that all animals originate from an egg Marconi Malpighi (late 1600s) – drew 1st micrographs of developing chick embryos – ignited debate between preformationist and epigenesists Early “Embryologists”
- 12. Preformation – Embryonic structures are preformed within the gamete (either egg or sperm) Epigenesis – the embryonic structures arise anew from the interaction of substances within the gametes Preformation vs Epigenesis
- 13. Malpighi found embryonic structures in “unincubated” eggs – these eggs were left in the warm sun of southern Italy during the summer months – So although they were unincubated, they were not unfertilized and some embryonic development occurred Malpighi’s observation spurned the preformationist Preformation vs Epigenesis
- 14. Preformationist vs Epigenesis Unusual Hypotheses of Preformationists – a tiny preformed being present in the gamete • This being would also have even tinier beings within it’s embryonic germ cells – and those tinier beings would have tinier still beings within their gametes » and so on and so on » and so on and so on » and so on and so on » and so on and so on » and so on and so on
- 15. Pervasiveness of preformationist ideas – Fertilization had never been observed – There was as yet (1600s) no cell theory to give a lower limit to the size a cell could have Preformation vs Epigenesis
- 16. Christian Pander – visualized epigenesis of embryonic germ layers Karl Ernst von Baer – described notochord, discovered mammalian egg, proposed 4 laws of development Heinrich Rathke – described pharyngeal arches, skull formation The First Embryologists ca1820
- 17. First Embryologists Germ Layers (Pander)
- 18. 1. general characters of a [genus] appear earlier during development than characters of [species] 2. more specific characters derive from more general characters 3. the embryo of a given species diverges form the adult form of lower animals rather than passing through the adult forms of lower animals 4. early embryos of higher animals never looks like a lower animal, but does resemble the embryo of lower animals Ernst von Baer’s Laws
- 19. First Embryologists Rathke’s Skull Studies
- 20. Lineage Tracing Observations of pigmented cells in living Tunicate embryos (E.G. Conklin, 1905)
- 21. Conklin’s Drawings of Tunicate Embryogenesis: Tracing Muscle Lineage
- 22. Lineage Tracing in Amphibian Embryos with Vital Dyes
- 23. Lineage Tracing in Zebrafish with Fluorescent Dyes
- 24. Lineage Tracing in Xenopus with Fluorescent Dyes