1.
Age Reversal Update
Healthy Masters 2019
Portugal
William Faloon

2.
age-reversal.net
This entire presentation and info
about our research available at:

3.
“A triple drug
combination has been
used to extend the
lifespan of fruit flies by
48% in a new study.”
https://www.nextbigfuture.com/2019/10/rapamycin-and-two-other-drugs-extends-lifespan-twice-as-much-as-any-other-drug-combo.html
Rapamycin and Two Other Drugs Extends Lifespan
Twice As Much As Any Other Drug Combo

4.
“Previousstudiesinfruitflieshave
achievedlifespan extensions ofabout
5-20%
…sowefounditwasquiteremarkable
thatthisdrugcombinationenabled
themtolive
Castillo-Quan, Jorge Iván, Luke S. Tain, Kerri J. Kinghorn, Li Li, Sebastian Grönke, Yvonne
Hinze, T. Keith Blackwell, Ivana Bjedov, and Linda Partridge. "A triple drug combination
targeting components of the nutrient-sensing network maximizes longevity."
Proceedings of the National Academy of Sciences 116, no. 42 (2019): 20817-20819.
September 30, 2019
Combined Interventions More Effective than SingleAgent Therapy

5.
PNAS October 15, 2019 116 (42) 20817-20819; first published September 30, 2019
https://doi.org/10.1073/pnas.1913212116
Rapamycin Lithium Dasatinib-like Drug+ +
Fruit Fly Lifespan Extension by 48%
=
“…a combination of drugs already approved for
human use is a viable strategy to maximize animal
longevity…”
This recent study supports the stairstep approach advocated to enable us to live significantly longer
September 30, 2019

6.
Fruit flies received drugs separately and/or in combination.
 Each drug individually extended lifespan by 11%
 Pairing two drugs extended lifespan roughly 30%
 Three drugs combined extended lifespan by 48%
Castillo-Quan, Jorge Iván, Luke S. Tain, Kerri J. Kinghorn, Li Li, Sebastian Grönke, Yvonne Hinze, T. Keith Blackwell, Ivana
Bjedov, and Linda Partridge. "A triple drug combination targeting components of the nutrient-sensing network
maximizes longevity." Proceedings of the National Academy of Sciences 116, no. 42 (2019): 20817-20819.
Combination Treatments are Essential
September 30, 2019

7.
Interventions that may
improve your odds of
living to year 2030

8.
…they are optimistic that a
person’s biological age can
be reversed.”
Age markers reversed by 2.5 years!
“ScientistsatInterveneImmuneandStanford
MedicalCenterhaveproventhat‘epigenetic
agingcanbereversedinhumans’...
Cocktail Of Drugs Gives First Hope
That ‘Biological Age’ Can Be Reversed
https://forbes.com/sites/robinseatonjefferson/2019/09/09/cocktail-of-drugs-gives-first-hope-that-biological-age-can-be-reversed/

9.
“Reversal of epigenetic aging and immunosenescent trends in humans.” Aging Cell; Sept 2019
Combination Therapy also Effective in Humans
1) Human growth hormone
2) DHEA
3) Metformin
Study conducted by Dr. Greg Fahy in collaboration
with researchers from Stanford University and
UCLA consisted of individualized doses of:
Study subjects also provided with daily
vitamin D3 and zinc.

10.
https://www.dailymail.co.uk/health/article-7435427/Aging-
REVERSED-small-group-men-study-reveals.htmll
Oct.22,2019Turning Back Time!
“Aging is REVERSED in
men using a cocktail of
growth hormones and
diabetes drugs in study
that saw test group shed
2.5 biological years.”

11.
Type II diabetics taking metformin:
United Kingdom Diabetes Study
►32% reduced risk of diabetic complications
►42% reduced risk for diabetes-related death
►36% reduced all-cause mortality risk
Study Results Found in January 1995
British Medical Journal; Jan 14, 1995;” United Kingdom Prospective Diabetes Study (UKPDS)

12.
Study Results Found on March 21st 2017
Journal of the American Medical Association
JAMA. Published online March 21, 2017. doi:10.1001/jama.2016.17844
Metformin studied in prediabetic patients. Compared
to placebo 850 mg of metformin two times a day:
Reduced type II diabetes risk 31%
In patients under age 60: metformin reduced diabetic risk by 58%.
“Metformin can cause weight loss…”Quote:

13.
Sept. 2017
Metformin Demonstrates Therapeutic
Effects Against Colon Cancer
Am J Med Sci. 2017 Sep;354(3):246-251. doi: 10.1016/j.amjms.2017.05.006. Epub 2017 May 19.
Metformin Has Positive Therapeutic Effects in Colon Cancer and Lung Cancer.
Colon Cancer Metformin Patients Non-Metformin Patients
Recurrences 4% 19%
Metastases 23% 46%
5-Year Survival 57% 37%
Deaths 48% 76%
(Metformin boosts AMPK activity and indirectly inhibits mTOR)
Conclusion: “Metformin therapy is associated with significantly better prognosis in patients with colon cancer”
Caveat: Some diabetics in control group treated with insulin or sulfonylurea drugs that promote tumor cell propagation.
Diabetics on metformin showed these benefits compared to diabetics not prescribed metformin:

14.
Diabetics taking metformin
have lower cancer rates
Metformin Prevents Cancer
MD Anderson Cancer Center found
risk of pancreatic cancer was 62%
lower in diabetics using metformin.
Li D, Yeung SC, Hassan MM, Konopleva M, Abbruzzese JL. Antidiabetic therapies
affect risk of pancreatic cancer. Gastroenterology. 2009 Aug;137(2):482-8.

15.
Nov 29. 2015

16.
Metformin Anti-aging Trial Gets Green-light
https://www.longevity.technology/worlds-first-anti-aging-trial-gets-green-light/
Sept 4, 2019
1958 - Metformin approved as anti-diabetic drug in England
1995 - Life Extension® lists metformin as anti-aging drug
2015 - FDA approves first anti-aging study of a drug (metformin)
2019 - Metformin study receives $75 million funding
($40 million from anonymous donor)

17.
Life Extension recommended
metformin 24 years ago…
the FDA went berserk!
March 1995

18.
Catastrophic Loss of Life
A 2019 study tabulated reductions in cardiovascular mortality in
type II diabetics using metformin.1
Life Extension® then calculated how many cardiovascular deaths
may have occurred in response to metformin’s 37-year delay.
Almost 4 million American diabetics may have died because of
the delay in this one drug (metformin) becoming available.
This exceeds the death toll of all wars America has ever fought.
1. Association of Treatment With Metformin vs Sulfonylurea With Major Adverse Cardiovascular
Events Among Patients With Diabetes and Reduced Kidney Function. JAMA. 2019 September 19:1-11.

19.
We Don’t Have Time for
Delayed Age Reversal

20.
Statistically Significant + Meaningful
Age Reversal in 12 Months
Outcome Objectives:
$550,000 + donated/discounted services
Human Age Reversal Project
Vitality in Aging Interventions Trial
Study Cost:
Major Funding Provided by:

21.
Fund research with tax deductible donations:
Human Age Reversal Project
Make checks payable to:
Human Age Reversal Project
300 NE 20th St. #409
Boca Raton, FL 33431
Or donate online:
age-reversal.net/donate

22.
Age Reversal Interventions
1)Target 5 calorie restriction pathways
2) Reduce/resolve inflammation
3) Telomere lengthening
4) Selective remove senescent cells
5) Restore youthful methylation
age-reversal.net

23.
VIA Interventions Trial
Price per Study
Subject:
ZERO
Estimated Value
per Study Subject:
>$10,000

24.
Age Reversal
Interventions
Autophagy Resolve inflammation
Remove
senescent cells
Restore
youthful methylation
Lengthen
telomeres
AMPK/
mTOR
Sirtuins -
NAD+
Exosomes

25.
Trial Parameters
> Open label (no placebo group)
> 50 healthy people aged 50-75 years
Initial Phase
Recruitment/Baseline Testing:
October 3rd 2019 – December 15th 2019
Vitality in Aging Intervention Trial

26.
Vitality in Aging Interventions Trial
Timeline

27.
Vitality in Aging Interventions Trial
Intervention #1
Calorie Restriction Mimetics

28.
12% Calorie Reduction Improves Biological Age Scores by 1.5 Years
2018 Published Human study measures 8 Aging Biomarkers
Change in the Rate of Biological Aging in Response to Caloric Restriction: CALERIE Biobank Analysis, J Gerontol A Biol Sci Med Sci. 2018 Jan; 73(1): 4-10.

29.
Intervention:1 Targeting Calorie Restriction Pathways
Metformin
Metformin
Rapamycin, metformin
Pterostilbene, nicotinamide
Nicotinamide riboside
Rapamycin, glucosamine
Beta-lapachone
↓Insulin/IGF-1
↑AMPK
↓mTOR
↑Sirtuins
↑ NAD+
↑Autophagy
↑NAD/NADH ratio

30.
VIA Interventions Trial
Increase
AMPK
Autophagy
Sirtuins/NAD+
Decrease
mTOR
Insulin
IGF-1 signals
Targets Calorie Restriction Pathways

31.
Vitality in Aging Interventions Trial
Senolytics
Selective removal of senescent cells
Intervention #2

32.
July 9, 2018
Magazine
“It’slooking like veryoldmice are able to substantially improve their health
span, reduce or delay age-related diseases and increase their survival.”
They calculated that if only one in 7,000 to 15,000 cells is senescent, then age-related
problems in physical function started to appear in the mice.
Like a contagion, senescent cells seem to pass on their accelerated aging abilities to healthy
cells by releasing a number of factors that can cause tissues like muscle to deteriorate.
Mice given senescent cells and the senolytic compounds lived 36% longer than animals
with senescent cell transplants who were not given the drugs.
How Scientists Are Testing
Cancer Drugs to Slow Down Aging

33.
Drug to clear 'zombie cells' from body could be first
anti-aging treatment after ‘impressive’ human trial
https://www.telegraph.co.uk/science/2019/01/08/drug-clear-zombie-cells-body-could-first-anti-ageing-treatment/
Jan. 8, 2019
“A drug to fight aging may finally
be on the horizon after the
first trial in humans showed
‘impressive’ results.”
“The treatment clears out dead
cells even when the immune
system no longer can.”

34.
This drug cocktail reduced signs of age-related
diseases and extended life in mice and human cells
Senolytic Delay May = Death
“Group led by Mayo Clinic anti-aging researcher James Kirkland not only offers a clear look at
the power of senescent cells to drive the aging process, but also a pharmaceutical cocktail that,
in mice at least, can slow and even reverse it.
Compared to mice who aged normally, those who started getting the dasatinib-quercetin cocktail at
an age equivalent to 75 to 90 years in humans ended up living roughly 36% longer, and with better
physical function.
In human cells in a test tube and in mice bearing human senescent cells, the dasatinib-quercetin
cocktail showed equally promising results, targeting senescent cells while leaving other cells intact.
Aging…is beginning to look more and more like a disease — and a treatable one at that.
ScientistsAre Testing
CancerDrugstoSlowDownAging July 10, 2019

35.
SEPTEMBER 17, 2018
Scientific Discovery and the Future of Medicine
Journal of the American Medical Association
“…many human pathologic conditions are associated with the presence of
senescent cells.”
“Interventions aimed at eliminating those senescent cells, commonly called
senolytic, have also been shown to improve health and extend life in various
mouse disease models.
“If senolytics are shown to be safe and effective in humans, they could transform
care of older adults and patients with multiple chronic diseases.”
Reference: “Aging, Cell Senescence, and Chronic Disease:Emerging Therapeutic Strategies.”;
Journal of the American Medical Association (JAMA) September 17th 2018.

36.
“Moreover, tremendous advances are being made in our
understanding of the science of aging, yielding exciting potential
treatments that may alter the course of this inevitable process.5 One
aspect of this work relates to “senolytics,” the study of ways to
either eliminate senescent cells that may have deleterious effects or
slow or reverse the aging process by restoring cells’ lost function.
“Enabling Healthful Aging for All —
The National Academy of Medicine Grand Challenge in Healthy Longevity”
https://www.nejm.org/doi/full/10.1056/NEJMp1912298?query=TOC
October 31, 2019
Purging failed brain insulation cells lessened damage,
improved cognition in Alzheimer's mouse model

37.
Selective removal of senescent cells - Age-related accumulation
of senescent cells contributes to atherosclerosis, aging of the
eye (cataracts, AMD), osteoporosis, skin and brain aging,
Kirkland and colleagues at Mayo Clinic show that senescent cells
are selectively eliminated by “senolytic” drugs.
Senolytic Cocktail of:
Senolytics
1) Dasatinib
2) Quercetin
3) Fisetin

38.
Suppress Chronic
Inflammation
Intervention #3:

39.
1) Fish oil: Boost omega-3 index > 10%
Reduce and Resolve Chronic Inflammation
2) Resolvins: Return tissues to health
Inflammaging major cause of aging and contributes to the global
loss of Sirt1 function. We intend to use omega-3 fatty acids and
other fatty acids to increase resolvins, protectins, and maresins
with a target of a minimum of 10% omega-3 index.

40.
Telomere
Elongation
Intervention #4:

41.
Telomereshortening normally
occurs atarateof0.9%/year.
Telomere Lengthening
1) Exercise
2) Stress Control
3) Healthy Diet
4) Omega-3 fatty acids
Onesmallprospectivecontrolledstudyinolder
menshowedthattelomerescouldbelengthened
by2%peryearwithacombinationof:

42.
Restoring
Youthful
Methylation
Intervention #5

43.
“TheroleofDNA methylationin epigeneticsofaging”
 Epigenetics, especially DNA methylation,
plays a mechanistic role in aging.
 Epigenetic clocks are best biomarkers for
predicting human mortality.
 DNAmethylationinfluencedbymetformin,caloric
restriction and rapamycin treatment in mice.
 This may reverse/prevent 20% to 40% of the
age-related changes in DNA methylation.
Pharmacol Ther. 2019 March; 195:172-185. doi:10.1016/j.pharmthera.2018.11.001

44.
Methods of Balancing Methylation
Pharmacol Ther. 2019 March; 195:172-185. doi:10.1016/j.pharmthera.2018.11.001
Metformin
CalorieRestrictionMimetics
Rapamycin

45.
LS/tandemmassspectrometrybasedtesting
Quantitativeb-galactosidasestaining,p16INK4a,etc)
Neurovision – Mayo Clinic CD38
Johns Hopkins
Aging Biomarker Tests
Vitality in Aging Interventions Trial
More Accurate FlowFISH-
Based Telomere Length Tests
Newer DNA
Methylation Age Tests
NAD+, NAD/NADH ratio,
and NAD+ metabolite tests
Cell Senescence Tests
Univ of British Columbia/UCLA Galactosidasestaining,p16INK4a,skinpunch
NanoSomiX
Exosome tests for predicting
Alzheimer’s disease 10 years
prior to onset of memory loss
Age Management Panel
Comprehensive blood
biomarkers with Levine
Biological Age Calculator

46.
Healthy Subjects in order to Measure Meaningful Age Reversal in 12 Months
Strict Inclusion Criteria for Study Subjects
Vitality in Aging Interventions Trial
Exercise
(10,000 step equivalent each day)
Healthy diet patterns
(no junk foods)
Restful sleep patterns
(no benzodiazepines)
Body Mass Index: 18-24
(no significant central fat mass)
No serious
preexisting disorders
No prescription narcotics
or recreational drugs
Target LDL below 80 mg/dl
Omega-3 Index > 8%
(achievablewithhighdosefishoil)

47.
Long Term Funding Sources Will Be Needed
Human Age Reversal Project
Bill Faloon
Donations of Physicians’ Time
Funding Need: > $550,000
Donations Received to Date: >$625,000
20
Vitality In Aging Interventions Trial
SOURCES OF FUNDING:

48.
New charity to fund research with tax deductible donations:
Human Age Reversal Project
Make checks payable to:
Human Age Reversal Project
300 NE 20th St. #409
Boca Raton, FL 33431
Or donate online:
age-reversal.net/donate

49.
Omega-3 IndexLiver-Kidney Function
Cardiovascular
Inflammatory
Hormones
Lipids (full-spectrum)
NAD+
Glycemic Markers Blood Cell Counts
Vitamin D
Insulin Resistance
Growth Factors

50.
NicotinamideFull ADPR
NAAD
ADPR
NAMN
NAD + metabolites PADPH
NAM
MeNAM
NADP+

51.
age-reversal.net
This entire presentation available at:

52.
Interventions that may
improve your odds of
living to year 2030

53.
Lifespans
not limited by
senescence
Neocortex Connects-
Converts to the Cloud
Years 2030 2050
Stair Step Approach to Infinite Longevity
Singularity + A.I. enables
limitless self-improvement.

54.
Hotel magnate Barron Hilton died
of natural causes at the age of 91.
He left about 97% of his estate to
Conrad N. Hilton Foundation, which
expects to grow its endowment
from $2.9 billion to $6.3 billion
www.foxbusiness.com/business-leaders/barron-hilton-fortune-estate-foundation
Who missed the longevity boat?
Barron Hilton (1927-2019)
Expiration date: Sept. 19, 2019
Net worth > $2.9 billion
Paris Hilton Barron Hilton

55.
Too Wealthy to Die at Age 65
Paul Allen, Chairman of the Seattle
Seahawks co-founded Microsoft in 1975.
Diagnosed and treated for non-Hodgkin
lymphoma in 2009. Cancer returned in
2018. He died from septic shock.
Paul Allen was philanthropist and
donated to medical research…
but did NOT make it his priority!
https://www.nytimes.com/2018/10/15/obituaries/paul-allen-dead.html
Paul Allen (1953-2018)
Net worth > $26.1 billion
Expiration date: Oct. 15, 2018

56.
Too Wealthy to Die at Age 58
Blake Nordstrom, company co-president,
dies unexpectedly at 58 from lymphoma.
At the time, he described his
condition as "treatable" and said he
planned to "continue to work
throughout this process as normal."
https://www.cnn.com/2019/01/02/business/blake-nordstrom-obit/
Blake Nordstrom(1961-2019)
Net worth > $1.5 billion
Expiration date: Jan. 2, 2019

57.
Who Missed the Longevity Boat
Robert "Bob" McNair, billionaire
founder and owner of the
Houston Texans died at age 81.
He battled squamous cell
skin cancer and then
leukemia for about 20 years.
https://www.forbes.com/profile/robert-mcnair/#130c78d9251a
RobertMcNair (1937-2018)
Net worth ~ $3.8 billion
Expiration date: Nov. 23, 2018
Wealthy And Famous
That Expired In 2018

58.
Who Missed the Longevity Boat
Herb Kelleher, the eccentric
founder of Southwest Airlines
who helped revolutionize
low-cost air travel in 1971.
Died at age 87.
https://www.cnn.com/2019/01/03/business/southwest-airlines-founder-herb-kelleher-obit
Herb Kelleher(1931-2019)
Net worth ~ $2.5 billion
Expiration date: Jan. 3, 2019
Wealthy And Famous
That Expired In 2019

59.
“Just a year ago I felt
immortal, wearing my
age with pride, even
joking about it.”
https://www.nytimes.com/2019/09/11/business/boone-pickens-dead.html
Who missed the longevity boat?
T. Boone Pickens (1928-2018)
Expiration date: Sept. 11, 2019
Net worth > $950 million
In 2016 T. Boone Pickens wrote:
One year later suffered series of
strokes and a fall. Dead at age 91.

60.
T. Boone Pickens Largely Funded $260 Million
Oklahoma State University Stadium
(He made many other charitable donations to biomedical research)

61.
Fund research with tax deductible donations:
Human Age Reversal Project
Make checks payable to:
Human Age Reversal Project
300 NE 20th St. #409
Boca Raton, FL 33431
Or donate online:
age-reversal.net/donate

62.
November,2009
No Human Age-Reversal
Intervention Identified
We Were Doomed 10 Years Ago

63.
Huge Advances in Only 10 Years
No Way to Reverse Aging
Manhattan Beach Summit Attendees
November 2009
Many Potential Methods
2009 2019

64.
2009-2019
Senolytics NAD+ Sirtuins mTOR-inhibition
AMPK
Activation
Umbilical Cord
Plasma/Blood
Exosomes from
Young Cells
Mesenchymal
Stem Cells
Immune
Restoration
Mitochondria
Restoration
Multiple Regenerative Interventions

65.
age-reversal.net
This entire presentation available at:

66.
Fund research with tax deductible donations:
Human Age Reversal Project
Make checks payable to:
Human Age Reversal Project
300 NE 20th St. #409
Boca Raton, FL 33431
Or donate online:
age-reversal.net/donate

67.
http://med.miami.edu/news/interdisciplinary-stem-cell-institute-at-the-university-of-miami-receives-2
Interdisciplinary Stem Cell Institute
at the University of Miami Receives $25 Million Donation
“The Interdisciplinary Stem Cell Institute at
the University of Miami Miller School of
Medicine has received a $25 million
donation from the Soffer Family Foundation.”
“The funding will allow the institute to
continue its groundbreaking stem cell
research… in fields of cardiology, sports
medicine, and aging-related conditions.”
October 8th 2019

68.
Unparalleled Track Record
of Biomedical Innovation
Established 1977

69.
age-reversal.net
This entire presentation and info
about our research available at:

70.
• END