Age Reversal Update from Bill Faloon on December 15th 2020

1. These slides can be viewed at: www.age-reversal.net AgeReversalUpdate William Faloon December 15th 2020

2. December 1, 2020 “Drug reverses age-related cognitive decline within days” DOI: 10.7554/eLife.62048 UC San Francisco scientists show a few doses of new drug:  Rapid restoration of cognitive function in aged mice  Reverse cognitive impairments in Down Syndrome  Restore memory function after traumatic brain injury  Prevent noise-related hearing loss  Immune cell rejuvenation  Enhanced cognition in healthy mice https://medicalxpress.com/news/2020-12-drug-reverses-age-related-cognitive-decline.html “Just a few doses of an experimental drug can reverse age- related declines in memory and mental flexibility in mice”

3. November 15, 2020 “Plasma dilution improves cognition and attenuates neuroinflammation in old mice” GeroScience Official Journal of the American Aging Association (AGE) https://link.springer.com/article/10.1007/s11357-020-00297-8 “Explaining the mechanism of the positive effects of neutral blood exchange on the brain, our comparative proteomics analysis demonstrated that dilution of old blood plasma yields an increase in the determinants of brain maintenance and repair in mice and in people. These findings confirm the paradigm of rejuvenation through dilution of age-elevated systemic factors and extrapolate it to brain health and function.”

4. Nature 588, 34-36 (2020doi: https://doi.org/10.1038/d41586-020-03119-1) Restoring Vision in Mice via Axon Regeneration natur e December 2 2020 Restoring vision in mice. Retinal ganglion cells (RGCs) transmit visual information from the eye to the brain along projections called axons. Damage to the RGC axons prevents transmission of this information, leading to sight loss. Lu et al.2 report that treatment of damaged RGCs with a transcription-factor cocktail called OSK restores the cells to a youthful state, leading to axon regeneration and restoration of sight in mice.

5. Nature 588, 34-36 (2020doi: https://doi.org/10.1038/d41586-020-03119-1) “Sight restored by turning back the epigenetic clo natur e December 2 2020  Genetically engineered viruses induce expression of three of the ‘Yamanaka factors’ that trigger mature cells to adopt an immature state.  Yamanaka expressed viruses triggered regeneration and long-distance axon extension following damage to the optic nerve.  Regenerative effects mediated by resetting DNA-methylation patterns.

6. Sept 30, 2020 British billionaire Jim Mellon plans to take his life extension start-up public in six to 12 months Juvenescence has 29 employees in London, has raised about $170 million privately and still has a “fair amount of money left in the bank.” Other billionaires, including Mike Cannon-Brookes, the co-founder of Australian software firm Atlassian, and NEX Group founder Michael Spencer, have invested in the company. Mellon isn’t the only billionaire who wants to live beyond a hundred. Michael Bloomberg, told Business Insider in 2017 he wants to live until he’s 125. https://www.cnbc.com/2020/09/29/billionaire-jim-mellon-plans-to-take-life-extension-start-up-public.html#:~:text=LONDON%20%E2%80%93%20British%20billionaire%20Jim%20Mellon,to%20extend%20the%20human%20life. Jim Mellon Born: February 1957

7. https://medicalxpress.com/news/2020-11-hyperbaric-oxygen-treatments-reverse-aging.html “Study finds hyperbaric oxygen treatments reverse aging process” A new study from Tel Aviv University and the Shamir Medical Center in Israel indicates that hyperbaric oxygen treatments in healthy aging adults can stop the aging of blood cells and reverse the aging process. In the biological sense, the adults' blood cells actually grow younger as the treatments progress. November 20, 2020 v Tel Aviv University

8. Research Paper Hyperbaric oxygen therapy increases telomere length and decreases immunosenescence in isolated blood cells : a prospective trial Yafit Hachmo1, * , Amir Hadanny2,3,4, * , Ramzia Abu Hamed1 , Malka Daniel- Kotovsky2 , Merav Catalogna2 , Gregory Fishlev2 , Erez Lang2 , Nir Polak2 , Keren Doenyas2 , Mony Fri edman2 , Yonatan Zemel2 , Yair Bechor2 , Shai Efrati1,2,3,5 •1 Research and Development Unit, Shamir Medical Center, Zerifin, Israel •2 The Sagol Center for Hyperbaric Medicine and Research, Shamir (Assaf-Harofeh) Medical Center, Zerifin, Israel •3 Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel •4 Bar Ilan University, Ramat-Gan, Israel •5 Sagol School of Neuroscience, Tel-Aviv University, Tel-Aviv, Israel * Equal contribution https://www.aging-us.com/article/202188/text AgING November 18, 2020

9. “Human ageing process biologically reversed in world first” 1, https://www.telegraph.co.uk/news/2020/11/18/human-ageing-process-biologically-reversed-world- first/#:~:text=The%20ageing%20process%20has%20been,that%20comes%20with%20growing%20older November 18, 2020 “The aging process has been biologically reversed for the first time by giving humans oxygen therapy in a pressurized chamber.”

10. November 19, 2020 “It Sure Looks Like Humans Have Found a Way to Reverse Aging” https://www.popularmechanics.com/science/health/a34730692/study-reverse-aging-in-humans/ “A landmark study shows the reversal of biological aging in humans.” “The researchers used oxygen therapy in a pressurized chamber to reverse aging in two key biological clocks.” “The study showed lengthening in the telomeres of chromosomes and a decrease in (senescent) cells known to cause aging.”

11. Novel Hyperbaric Oxygen Protocol Extends Telomere Length and Improves Immune Markers Some immune cell telomeres elongated by 20% Some senescent immune cells reduced by 37% Improved immune markers. https://www.aging-us.com/article/202188/text

12. Improved Immune Cell Telomere Lengths in Response to Hyperbaric Therapy

13. Decrease in Senescent Immune Cells in Response to Hyperbaric Therapy

14. “Human ageing process biologically reversed in world first” 1, https://www.telegraph.co.uk/news/2020/11/18/human-ageing-process-biologically-reversed-world- first/#:~:text=The%20ageing%20process%20has%20been,that%20comes%20with%20growing%20older November 18, 2020 “Scientists said the growth may mean that the telomeres of trial participants were now as long as they had been 25 years earlier.”

15. Hyperbaric Treatment Protocol  Five daily sessions a week for 3 months  Each session involved breathing 100% oxygen by mask for 90 minutes with 5- minute air breaks every 20 minutes.  Each total session time about 2 hours.  Commercial price for 60 sessions: $18,000 https://www.aging-us.com/article/202188/text

16. Authors of Hyperbaric Study Describe Other Methods Shown to Preserve Telomere Length High consumption of omega-3s Diet rich in walnuts Calorie restriction https://www.aging-us.com/article/202188/text Healthy lifestyles High intensity interval training Antioxidants

17. Anti-Aging Clinical Trials Building 200, Suite 205 13940 US Hwy 441 The Villages, FL 32159 Betterhumans.org

18. June 2020 First Hyperbaric Chamber Clinic of its type opens in The Villages in North-Central Florida. https://www.83degreesmedia.com/innovationnews/Aviv-clinics-presents-HBOT-at-innovation-fustion-event-in-tampa-092120.aspx

19. “Overhyping the Effects of Hyperbaric Oxygen Treatment on Aging” November 20th, 2020 https://www.fightaging.org/archives/2020/11/overhyping-the-effects-of-hyperbaric-oxygen-treatment-on-aging/ FIGHT AGING! “Hence there are media articles out there at the moment breathlessly telling us that hyperbaric oxygen treatment reverses aging. This is ridiculous, and only makes it harder for the better end of the industry to make progress.”

20. December 17, 1903 First flight: 120 feet/20 feet off ground

21. December 20, 1957 Intercontinental Air Travel 54 Years after Wright Brothers first-in flight

22. Level 1 is essential to progress to Level 2. Optimal Health Level 2 Level 1 - Hormones - Renal function - LDL/HDL - Inflamation - Blood Pressure - Glucose - C-reactive protein - Homocysteine - Insulin - Coagulation Correct Your Underlying Disease Risk Factors Age Reversal Interventions

23. Time Restricted Eating (Intermittent Fasting) Update

24. https://www.nejm.org/doi/full/10.1056/NEJMra1905136 Dec. 26, 2019 “Evidence is accumulating that eating in a 6-hour period and fasting for 18 hours can trigger a metabolic switch from glucose-based to ketone- based energy, with increased stress resistance, increased longevity, and a decreased incidence of diseases, including cancer and obesity.” EffectsofIntermittentFastingonHealth,Aging,andDisease

25. https://www.nejm.org/doi/full/10.1056/NEJMra1905136 EffectsofIntermittentFastingonHealth,Aging,andDisease Suppresses inflammation Reduces obesity and type II diabetes Protects against cardiovascular disease Lowers risk of neurodegenerative disorders Reduces cancer incidence Animal models show consistent robust benefits of intermittent fasting: Dec. 26, 2019

26. https://www.nejm.org/doi/full/10.1056/NEJMra1905136 Human studies show intermittent fasting ameliorates: Dec. 26, 2019  Obesity  Dyslipidemia  Hypertension  Inflammation  Insulin resistance One study found a 57% decrease in fasting insulin levels Effects of Intermittent Fasting on Health, Aging, and Disease

27. SEPTEMBER 28, 2020 JAMA Study Shows Little Intermittent Fasting Benefit Journal of the American Medical Association Robust benefits not found in a JAMA Internal Medicine Study designed to examine effects of intermittent fasting on weight loss and metabolic risk markers. Time-restricted eating (intermittent fasting) group lost a little weight over 12 weeks compared to control group. No significant effect on metabolic risk markers. We’ve long known about time-restricted eating does not induce much weight loss in those who do not also reduce their overall calorie intake. Preponderance of published evidence supports benefits of intermittent fasting. “Effects of Time-Restricted Eating on Weight Loss and Other Metabolic Parameters in Women and Men With Overweight and Obesity: The TREAT Randomized Clinical Trial.” JAMA Intern Med. 2020 Sep 28.

28. natureresearch October 27, 2020 Scientific Reports | (2020) 10:18341 | https://doi.org/10.1038/s41598-020-73767-w “Intermittent fasting from dawn to sunset for 4 consecutive weeks induces anticancer serum proteome response & improves metabolic syndrome” No eating/drinking between dawn and dusk…14-15 hours each day: • 7.25 pounds of weight loss • 8.8 mmHg reduction in blood pressure • Significant increase in tumor suppressor/anticancer proteins • Significant decrease in several tumor promoter/pro-cancer proteins • Increasing a protein called calreticulin (by around 16 times) • Calreticulin enhances IgG response to a SARS-CoV spike protein

29. Magazine September 22, 2020 s Aging a Disease You Can Reverse? A Look at the Science Behind the Longevity Movement “Scientists are saying aging is a disease to be targeted, treated, and perhaps even reversed. Longevity is the wellness world’s latest buzzword. The longevity sector is on track to be a multi- trillion-dollar industry.” https://www.vogue.com/article/how-to-live-longer-longevity?fbclid=IwAR3Pj2yYRqLwIwtm3LuboTfkRf2fubjLuybGiz9MpogJKO9UunyWDWiTZCU

30. December 4, 2015

31. May 2017

32. Blood from young animals can revitalize old ones July 15th, 2017

33. September 15th, 2017

34. https://www.pressreader.com/usa/popular-science/20180505/281672550562011 “Bill Faloon has pursued immortality for decades. Now he’s got lots of company. What does science have to say?” “The FOREVER Man” May 5, 2018

35. April 27, 2019 https://www.newscientist.com/issue/3227/

36. “The Science of Living Longer” “No longer is it (aging) being treated as a dreadful inevitability but instead as a puzzle that can be solved---as a disease with a cure.” TIME-Special Edition - Fall 2019

37. DEC. 8, 2019 “A Harvard geneticist's goal: to protect humans from viruses, genetic diseases, and aging” George Church's lab at Harvard Medical School is working to make humans immune to all viruses, eliminate genetic diseases and reverse the aging process…

38. October 17th 2020 “This Japanese Engineer Created the Robots That Make Your Created $40 Billion Company Dies in 2020 at age 95 Mr. Inaba read a 1952 article in Scientific American describing rudimentary computer program that told a machine how to carve up a piece of metal. He advanced this early MIT technology into “programmable machine tools” that enable today’s computer-driven assembly lines. https://www.wsj.com/articles/this-japanese-engineer-created-the-robots-that-make-your-cars-11602856803 Genesis was the Scientific American article!

39. https://www.technologyreview.com/magazine/2019/09 September 2019 “Later this year, at a conference of longevity enthusiasts called RAADfest, to be held in Las Vegas, Faloon plans to take the strategy one step further with what he has been calling the “Perpetual Clinical Trial.” “OLD AGE IS OVER!”

40. Targeting only one pathway of aging in humans will not produce a meaningful change in healthspan or longevity. Today’s Dilemma

41. Intervene with a cocktail of treatments to improve health as measured by markers of biological age. SOLUTION:

42. Age Reversal Interventions Autophagy Resolve inflammation Remove senescent cells Restore youthful methylation Lengthen telomeres AMPK/ mTOR Sirtuins - NAD+ Exosomes

43. VIA Interventions Trial Human Age Reversal Project pays lab tests + interventions. (50 people in this study) VIA Longitudinal Study You pay (discounted prices) for lab tests and interventions. (Everyone should enroll) To apply or enroll in VIA studies: VitalityInAging.org/

44. Human Age Reversal Project Goal: Statistically significant + real world age reversal in 12-15 months. Vitality in Aging Interventions Trial

45. Vitality in Aging Interventions Trial Objective: Evaluate a combination of compounds that individually have been shown to increase healthspan and/or lifespan in model organisms. To apply: VitalityInAging.org/

46. Reverse Aging with Combined Interventions Senolytics NAD+ Sirtuins mTOR AMPK Young Plasma Factors Stem Cells Restore Immune Function MitochondriaExosomes

47. (Need website for donations) Human Age Reversal Project $914,000 + $100,000 = $1,014,000 Donated + Pledged Funding for VIA Intervention Trial: Goal: Statistically Significant + Meaningful Age Reversal > One Million Funding by January 2021

48. Fund Research with Tax-Deductible Donations Human Age Reversal Project Make checks payable to: Human Age Reversal Project 300 NE 20th St. #409 Boca Raton, FL 33431 Or donate online: age-reversal.net/donate

49. VIA Interventions Trial Human Age Reversal Project pays lab tests + interventions. (50 people in this study) VIA Longitudinal Study You pay (discounted prices) for lab tests and interventions. (Everyone should enroll) To apply or enroll in VIA studies: VitalityInAging.org/

50. Vitality in Aging Longitudinal Study Join a team effort to defeat aging! Identify “risk factors” causing you to age Access biomarker tests at subsidized prices Access interventions at discount prices Test safety & efficacy of self-experimentation Accelerate science of human age reversal

51. Step 1 - Enroll in VIA Longitudinal Study - $495 Step 2 - Includes Age Management Blood Test Panel Step 3 - Use test results to adjust your longevity program Step 4 - Retest to measure age reversal efficacy Step 5 - Adjust interventions based on retest results Modeled after Framingham Heart Study, i.e. long term and ongoing… ToenrollinVIALongitudinal Study: VitalityInAging.org

52. Vitality in Aging Longitudinal Study 1) Age Management Blood Test Panel on an annual basis. ($495) 2) Completion of online surveys. 3) Mimic VIA Interventions Trial and/or choose your own protocol. 4) Additional surveys sent on specific topics. 5) This is a self-funded study with subsidized prices. To enroll: VitalityInAging.org/

53. Established 1977 to Needlessly Die” “No One is Allowed

54. Heart attack & stroke deaths increase relative to cancer in elderly persons. Reference: Heron, M. Deaths: Leading Causes for 2017. National Vital Statistics Reports. June 24, 2019; Volume 68, Number 6. https://www.cdc.gov/nchs/data/nvsr/nvsr68/nvsr68_06-508.pdf Heart Disease Still #1 Killer

55. Level 1 essential to sustain life Optimal Health Level 2 Level 1 - Hormones - Renal function - LDL/HDL - Vitamin D - Blood Pressure - Glucose - C-reactive protein - Homocysteine - Insulin - Coagulation Some Longevity Enthusiasts Fail To Correct Risk Factors Death = Failure Harsh Reality!

56. House of Optimal Health - Hormones - Kidney function - LDL/HDL - Vitamin D - Blood Pressure - Glucose - C-reactive protein - Homocysteine - Insulin - Coagulation Level 2 Level 1 - Senolytics - NAD+ Restoration - mTOR-inhibition - Umbilical Cord Blood/Plasma - Exosomes - Mesenchymal Stem Cells - Mitochondria Transfer - Young Plasma Failure to optimize Level 1, preclude benefits of Level 2. Age Reversal Interventions Healthy Aging

57. We Don’t Have Time to Delay Age Reversal

58. https://en.wikipedia.org/wiki/Sumner_Redstone Redstone built multi-billion-dollar empire. Donated >$216 million to charities. Boasted he would live forever. Dead at age 97 from dementia complications. Sumner Redstone 1923-2020 Expiration date: Aug. 11, 2020 Net worth > $3-4 billion

59. Risk of Delay: Chronic Diseases May Create Side Effects

60. Reverse Aging with Combined Interventions Senolytics NAD+ Sirtuins mTOR AMPK Young Plasma Factors Stem Cells Restore Immune Function MitochondriaExosomes

62. Maximum natural lifespan of bowhead whales is 268 years. DNA Methylation Test Reveals Bowhead Whales Longevity https://cosmosmagazine.com/biology/life-clock-ticking

63. Sequencing the genome of the bowhead whale was done to identify candidate genes to explain their long lifespan and resistance to diseases. This project helped elucidate genes conferring longevity and disease resistance that may to improve human health. Genomic Research Funding Supported by grants from Life Extension® and the Methuselah Foundation.

64. October 24, 2019 “GettingComfortablewithRisk” “...without daring innovators, disruptive thinkers, and voluntary patients, little medical progress could be made” https://www.nejm.org/doi/full/10.1056/NEJMp1906872

65. Unparalleled Track Record of Biomedical Innovation Established 1977

66. age-reversal.net This entire presentation available at:

67. https://www.youtube.com/watch?v=bP-FFQPlaT4 First Hyperbaric Chamber Clinic of its type opens in The Villages in North-Central Florida June 2020

Age Reversal Update from Bill Faloon on December 15th 2020

Max Peto

Age Reversal Update from Bill Faloon on December 15th 2020