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Specimen receiving
- 1. Biochemistry Lab Mr. MOHAMED A. ADAM Medical Lab Technologist KCCC
- 3. Samples Received with requests Centrifugation of samples Data are entered in the LIS to get barcode Barcodes are stick on the tubes then distributed to machine Sample Processing Results are Reported and Released Clean your bench with fresh actichlor
- 4. WHAT WE ARE RECEIVE? •Specimen type • Whole Blood 1. Plasma 2. Serum • Body Fluid 1. Cerebro-spinal Fluid CSF Cerebrospinal fluid (CSF) is a fluid that surrounds the brain and spinal cord. CSF acts as a cushion, protecting the brain and spine from injury. The fluid is normally clear same as water.
- 6. Phlebotomist draws sample 1.Gentlly invert five times To mix dot activator with blood 2. Allow blood to clot in a vertical position Observe a dense clot 3.Centrifuge for 10 min. at 3000 rpm
- 7. SEPARATION OF SAMPLES (SERUM) PRE-ANALYTICAL 3000 rpm 10 min Should be balanced
- 8. WHAT IS THE DIFFERENCE BETWEEN SERUM AND PLASMA
- 9. VACUTAINER BLOOD COLLECTION TUBES Range Color coding Additive Clinical Use for Whole blood EDTA Hematology test Special biochemistry (HbA1c) Immunochemistry (Cyclosporin and Tacrolimus) Plasma Lithium Heparin Cardiac Markers Sodium Fluoride + Potassium Oxalate Glucose Test (FBS free blood sugar) Sodium Citrate Coagulation test Serum Plain (NA additive) Clot activator Serum biochemistry and immunology test Tumor Markers Hormones Vitamin D (should be covered) Oncology RFT – LFT – Lipid profileGel + Clot activator
- 10. SAMPLE TRANSPORT AND STORAGE • Transport must be save Biohazardous material • 4 HOURS Stable at 15-25 ˚c Close to avoid evaporation • Stable at 2-8 ˚c Dry Ice – Cool packs – refrigerator .. etc 24 hours only (for some biochemistry analyte such as K, )
- 11. LIST OF TESTS IN OUR LAB TUMOR MARKERS Test Diagnosis CEA (No Spicificity) Colorectal, gastrointestinal, lung and breast cancers CA-15.3 Breast Cancer CA-125 Ovarian Cancer CA-19.9 Colorectal and Pancreatic cancer B2Microglobulin Blood cell cancer Test Diagnosis PSA Total and Free Prostate Cancer AFP Liver Cancer Cyfra 21-1 Lung Cancer TG Thyroid Cancer ATG B-HCG Chorion Cancer “Pregnant test”
- 12. LIST OF TESTS IN OUR LAB BIOCHEMISTRY Biochemistry Profiles Oncology (biochemistry _ Full Profile) Liver Electrolyte Lipid Amylase Renal Iron HbA1c Cardiac Markers CK – CKMB - Troponin Thyroid Function Test (TFT) TSH – FT4 Other Tests Vitamin D PCT Cyclosporin Tacrolimus
- 13. EACH SPECIMEN SHOULD BE LABELED WITH PATIENT`S INFORMATION • Full Name • Age • Gender • File number or Civil ID • Doctor`s Stamp and signature • Clear and Specific test should be written • Cashier Stamp (If needed) for Vitamin D , Hormones and TFT The personal information such (Name, file # or civil ID) MUST MATCH the same on the request