SlideShare a Scribd company logo
1 of 20
Download to read offline
BIOTIN (B7]
Gandham. Rajeev
Department of Biochemistry,
Akash Institute of Medical Sciences
& Research Centre,
Devanahalli, Bangalore,
Karnataka, India.
E-Mail: gandhamrajeev33@gmail.com
BIOTIN (B7)
BIOTIN (B7)
 Biotin is formerly known as anti-egg white injury
factor or vitamin H
 It is water soluble sulfur containing B-complex
vitamin
 Biotin mainly participates in the carboxylation
reactions
 Biotin (B7) is a heterocyclic sulfur containing
monocarboxylic acid
 Biotin is imidazole derivative
 It is formed by fusion of imidazole & thiophene
rings with a valeric acid side chain
 Biotin covalently bound to ε – amino group of
lysine to form biocytin
 Biocytin is the coenzyme form of Biotin
 Biotin is a prosthetic group of carboxylase
 Biotin is required for carboxylation reactions (CO2
fixation reaction)
 Biotin is required for the enzymes
 Pyruvate carboxylase
 Acetyl CoA carboxylase
 Propionyl carboxylase
 β - Methyl crotonyl CoA carboxylase
Pyruvate carboxylase
 Pyruvate carboxylase catalyzes the conversion of
pyruvate to oxaloacetate
Pyruvate
Oxaloacetate
CO2, ATP Pyruvate
carboxylase
BiotinADP+Pi
Mg++/Mn
Acetyl CoA carboxylase
 Acetyl CoA carboxylase catalyzes the formation of
malonyl CoA from acetyl CoA
 The reaction provides acetate molecule for
fattyacid synthesis
AcetylCoA
Malonyl CoA
CO2, ATP Acetyl CoA
carboxylase
BiotinADP+Pi
Mg++/Mn
Propionyl CoA Carboxylase
 Propionyl CoA carboxylase catalyzes the formation
of D – Methyl malonyl CoA from propionyl
CoA(from odd chain FA & methionine)
 It required for entry of Propionyl CoA to TCA cycle
via succinyl CoA
Propionyl CoA
D – Methyl malonyl CoA
CO2, ATP Propionyl CoA
carboxylase
BiotinADP+PiMg++/Mn
β - Methyl crotonyl CoA carboxylase
 β - Methyl crotonyl CoA carboxylase catalyzes the
formation of β – Methylglutaconyl CoA from β -
Methyl crotonyl CoA
 It essential for leucine catabolism
β - Methyl crotonyl CoA
β – Methylglutaconyl CoA
CO2, ATP β - Methyl crotonylCoA
carboxylase
BiotinADP+PiMg++/Mn
 All the carboxylation reactions in the biological
system are not dependent on Biotin
 Few carboxylation reactions which do not require
biotin
 Formation of carbamoyl phosphate in urea cycle
 Incorporation of CO2 in purine synthesis
 Rich sources are eggs, liver, kidney, & yeast,
pulses, nuts, vegetables
 Poor sources are cereals & dairy products
RDA
Adults - 200 – 300 mg/day
 Biotin deficiency is generally not seen in man
because of
 Its wide distribution in foods
 Synthesis of vitamin by the bacterial flora in the
gut
 Clinical features
 Severe dermatitis, weakness, & nausea
 In animals muscle weakness, dermatitis & loss of
hair around the eye
BIOTIN (B7)
BIOTIN (B7)
 Avidin ( Raw egg white injury factor )
 Raw egg white injury factor is a heat labile protein
known as avidin & is present in raw egg white
 Avidin binds to biotin & makes its unavailable for
absorption
 Avidin is inactivated by boiling the eggs & biotin is
readily absorbed when boiled eggs are used in the
diet
 One molecule of avidin can combine with four
molecules of biotin
 Egg white contains Avidin & egg yolk contains
biotin
 The affinity of Avidin to biotin is greater than most
of the usual antigen-antibody reactions
 Avidin-biotin system is commonly utilized for
detection of pathogenesis in ELISA test
 DNA is generally labelled by radioactive
nucleotides
 Recently, biotin labelling of DNA is becoming more
popular
 Biotin is added to nucleotides, which will be
incorporated into the newly synthesized DNA
 The fixed biotin can be identified by reaction with
Avidin
 Intake of 20 raw eggs/day will produce Biotin
deficiency in humans
 Prolonged use of antibacterial drugs such as
sulfonamides
References
 Harper’s Biochemistry 25th Edition.
 Fundamentals of Clinical Chemistry by Tietz.
 Text Book of Medical Biochemistry-A R Aroor.
 Text Book of Biochemistry-DM Vasudevan
 Text Book of Biochemistry-MN Chatterjea
 Text Book of Biochemistry-Dr.U.Satyanarana

More Related Content

What's hot (20)

Folic acid (B9)
Folic acid (B9)Folic acid (B9)
Folic acid (B9)
 
Vitamin E
Vitamin EVitamin E
Vitamin E
 
VITAMIN C
VITAMIN CVITAMIN C
VITAMIN C
 
Vitamin b9
Vitamin b9Vitamin b9
Vitamin b9
 
BIOTIN (B7)
BIOTIN (B7)BIOTIN (B7)
BIOTIN (B7)
 
Vitamin E
Vitamin E Vitamin E
Vitamin E
 
VITAMIN B6 PYRIDOXINE
VITAMIN B6  PYRIDOXINEVITAMIN B6  PYRIDOXINE
VITAMIN B6 PYRIDOXINE
 
Vitamin b1
Vitamin b1Vitamin b1
Vitamin b1
 
Vitamin b5, pantothenic acid
Vitamin b5, pantothenic acidVitamin b5, pantothenic acid
Vitamin b5, pantothenic acid
 
Vitamin B12
Vitamin B12Vitamin B12
Vitamin B12
 
Biotin ppt.
Biotin ppt.Biotin ppt.
Biotin ppt.
 
Biotin
BiotinBiotin
Biotin
 
Vitamin b6
Vitamin b6Vitamin b6
Vitamin b6
 
Vitamin b7 (biotin)
Vitamin b7 (biotin)Vitamin b7 (biotin)
Vitamin b7 (biotin)
 
VITAMIN A
VITAMIN AVITAMIN A
VITAMIN A
 
Biotin
BiotinBiotin
Biotin
 
Vitamin b7 or biotin
Vitamin b7 or biotinVitamin b7 or biotin
Vitamin b7 or biotin
 
Folic acid
Folic acid Folic acid
Folic acid
 
Biotin (vitamin b7) biological functions, clinical indications and its techn...
Biotin (vitamin b7)  biological functions, clinical indications and its techn...Biotin (vitamin b7)  biological functions, clinical indications and its techn...
Biotin (vitamin b7) biological functions, clinical indications and its techn...
 
Riboflavin ( B2) MUHAMMAD MUSTANSAR
Riboflavin ( B2) MUHAMMAD MUSTANSARRiboflavin ( B2) MUHAMMAD MUSTANSAR
Riboflavin ( B2) MUHAMMAD MUSTANSAR
 

Viewers also liked

Biotin ( vitamin B7) Egg white injury, Leiner's disease
Biotin ( vitamin B7) Egg white injury, Leiner's diseaseBiotin ( vitamin B7) Egg white injury, Leiner's disease
Biotin ( vitamin B7) Egg white injury, Leiner's diseaseIrma Suntoo
 
A presentation on pantothenic acid or b5
A presentation on pantothenic acid or b5A presentation on pantothenic acid or b5
A presentation on pantothenic acid or b5jainsaketjain
 
Decolourization of textile dye effluents
Decolourization of textile dye effluentsDecolourization of textile dye effluents
Decolourization of textile dye effluentsShameem_Byadgi
 
textile biodegradation
textile biodegradationtextile biodegradation
textile biodegradationfaysalgcuf
 
Cell to cell communication
Cell to cell communicationCell to cell communication
Cell to cell communicationLawrence James
 
autonomic nervous system Ppt
autonomic nervous system Pptautonomic nervous system Ppt
autonomic nervous system Pptdrbskamble
 

Viewers also liked (9)

Biotin ( vitamin B7) Egg white injury, Leiner's disease
Biotin ( vitamin B7) Egg white injury, Leiner's diseaseBiotin ( vitamin B7) Egg white injury, Leiner's disease
Biotin ( vitamin B7) Egg white injury, Leiner's disease
 
A presentation on pantothenic acid or b5
A presentation on pantothenic acid or b5A presentation on pantothenic acid or b5
A presentation on pantothenic acid or b5
 
Vitamina B7
Vitamina B7Vitamina B7
Vitamina B7
 
Xenobiotic metabolism
Xenobiotic metabolismXenobiotic metabolism
Xenobiotic metabolism
 
Decolourization of textile dye effluents
Decolourization of textile dye effluentsDecolourization of textile dye effluents
Decolourization of textile dye effluents
 
textile biodegradation
textile biodegradationtextile biodegradation
textile biodegradation
 
Cell to cell communication
Cell to cell communicationCell to cell communication
Cell to cell communication
 
autonomic nervous system Ppt
autonomic nervous system Pptautonomic nervous system Ppt
autonomic nervous system Ppt
 
Xenobiotics
XenobioticsXenobiotics
Xenobiotics
 

Similar to BIOTIN (B7) (20)

Biotin.pptxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Biotin.pptxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxBiotin.pptxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Biotin.pptxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
 
VITAMIN B8 BIOTIN MUHAMMAD MUSTANSAR
VITAMIN B8  BIOTIN   MUHAMMAD MUSTANSARVITAMIN B8  BIOTIN   MUHAMMAD MUSTANSAR
VITAMIN B8 BIOTIN MUHAMMAD MUSTANSAR
 
BIOTIN - UG CLASS 2018.pptx
BIOTIN - UG CLASS 2018.pptxBIOTIN - UG CLASS 2018.pptx
BIOTIN - UG CLASS 2018.pptx
 
Vitamin (overview of a,b & c)
Vitamin (overview of a,b & c)Vitamin (overview of a,b & c)
Vitamin (overview of a,b & c)
 
Vitamins
VitaminsVitamins
Vitamins
 
Vitamins
VitaminsVitamins
Vitamins
 
vitamins.pdf
vitamins.pdfvitamins.pdf
vitamins.pdf
 
Mel niacin (vitamin b3)
Mel niacin (vitamin b3)Mel niacin (vitamin b3)
Mel niacin (vitamin b3)
 
Water soluble vitamins
Water soluble vitaminsWater soluble vitamins
Water soluble vitamins
 
Bio water soluble-vitamins_by_dr_shivani_pandey-16-12-14
Bio water soluble-vitamins_by_dr_shivani_pandey-16-12-14Bio water soluble-vitamins_by_dr_shivani_pandey-16-12-14
Bio water soluble-vitamins_by_dr_shivani_pandey-16-12-14
 
water soluble vitamins..
water soluble vitamins..water soluble vitamins..
water soluble vitamins..
 
6. Vitamins.pptx
6. Vitamins.pptx6. Vitamins.pptx
6. Vitamins.pptx
 
Vitamins and coenymes
Vitamins and coenymesVitamins and coenymes
Vitamins and coenymes
 
Power Point Presentation on Vitamines
Power Point Presentation on VitaminesPower Point Presentation on Vitamines
Power Point Presentation on Vitamines
 
Water soluble vitamins.pptx
Water soluble vitamins.pptxWater soluble vitamins.pptx
Water soluble vitamins.pptx
 
Vitamins
VitaminsVitamins
Vitamins
 
FOOD SCIENCE AND NUTRITION
FOOD SCIENCE AND NUTRITIONFOOD SCIENCE AND NUTRITION
FOOD SCIENCE AND NUTRITION
 
Vitamins- steps to healthier you
Vitamins- steps to healthier youVitamins- steps to healthier you
Vitamins- steps to healthier you
 
unit12.2web.ppt
unit12.2web.pptunit12.2web.ppt
unit12.2web.ppt
 
Vitamins.pptx
Vitamins.pptxVitamins.pptx
Vitamins.pptx
 

More from YESANNA

PERICARDIAL FLUID
PERICARDIAL FLUIDPERICARDIAL FLUID
PERICARDIAL FLUIDYESANNA
 
SYNOVIAL FLUID
SYNOVIAL FLUID SYNOVIAL FLUID
SYNOVIAL FLUID YESANNA
 
ASCITIC FLUID ANALYSIS
ASCITIC FLUID ANALYSISASCITIC FLUID ANALYSIS
ASCITIC FLUID ANALYSISYESANNA
 
CEREBROSPINAL FLUID (CSF)
CEREBROSPINAL FLUID (CSF)CEREBROSPINAL FLUID (CSF)
CEREBROSPINAL FLUID (CSF)YESANNA
 
Oxidative Stress in Preeclampsia
Oxidative Stress in Preeclampsia Oxidative Stress in Preeclampsia
Oxidative Stress in Preeclampsia YESANNA
 
GANDHAM RAJEEV-BIOCHEMISTRY IMPORTANT QUESTIONS-RGUHS-2017
GANDHAM RAJEEV-BIOCHEMISTRY IMPORTANT QUESTIONS-RGUHS-2017GANDHAM RAJEEV-BIOCHEMISTRY IMPORTANT QUESTIONS-RGUHS-2017
GANDHAM RAJEEV-BIOCHEMISTRY IMPORTANT QUESTIONS-RGUHS-2017YESANNA
 
MINERALS-REVISION - 27-05-2017
MINERALS-REVISION - 27-05-2017MINERALS-REVISION - 27-05-2017
MINERALS-REVISION - 27-05-2017YESANNA
 
IRON METABOLISM
IRON METABOLISMIRON METABOLISM
IRON METABOLISMYESANNA
 
METABOLISM OF ZINC, MAGNESIUM & ELECTROLYTES
METABOLISM OF ZINC, MAGNESIUM & ELECTROLYTESMETABOLISM OF ZINC, MAGNESIUM & ELECTROLYTES
METABOLISM OF ZINC, MAGNESIUM & ELECTROLYTESYESANNA
 
METABOLISM OF SULFUR, IODINE, MANGANESE,FLUORINE & SELENIUM
METABOLISM OF SULFUR, IODINE, MANGANESE,FLUORINE & SELENIUMMETABOLISM OF SULFUR, IODINE, MANGANESE,FLUORINE & SELENIUM
METABOLISM OF SULFUR, IODINE, MANGANESE,FLUORINE & SELENIUMYESANNA
 
COPPER METABOLISM
COPPER METABOLISMCOPPER METABOLISM
COPPER METABOLISMYESANNA
 
MATABOLISM OF CALCIUM & PHOSPHOROUS
MATABOLISM OF CALCIUM & PHOSPHOROUSMATABOLISM OF CALCIUM & PHOSPHOROUS
MATABOLISM OF CALCIUM & PHOSPHOROUSYESANNA
 
VITAMIN LIKE COMPOUNDS
VITAMIN LIKE COMPOUNDS VITAMIN LIKE COMPOUNDS
VITAMIN LIKE COMPOUNDS YESANNA
 
COBALAMINE (12)
COBALAMINE (12) COBALAMINE (12)
COBALAMINE (12) YESANNA
 
FOLIC ACID (B9)
FOLIC ACID (B9)FOLIC ACID (B9)
FOLIC ACID (B9)YESANNA
 
PANTOTHENIC ACID (B5)
PANTOTHENIC ACID (B5)PANTOTHENIC ACID (B5)
PANTOTHENIC ACID (B5)YESANNA
 
VITAMIN K
VITAMIN KVITAMIN K
VITAMIN KYESANNA
 
VITAMIN D
VITAMIN D VITAMIN D
VITAMIN D YESANNA
 
VITAMIN E
VITAMIN EVITAMIN E
VITAMIN EYESANNA
 
Identification of unknown carbohydrate solution
Identification of unknown carbohydrate solutionIdentification of unknown carbohydrate solution
Identification of unknown carbohydrate solutionYESANNA
 

More from YESANNA (20)

PERICARDIAL FLUID
PERICARDIAL FLUIDPERICARDIAL FLUID
PERICARDIAL FLUID
 
SYNOVIAL FLUID
SYNOVIAL FLUID SYNOVIAL FLUID
SYNOVIAL FLUID
 
ASCITIC FLUID ANALYSIS
ASCITIC FLUID ANALYSISASCITIC FLUID ANALYSIS
ASCITIC FLUID ANALYSIS
 
CEREBROSPINAL FLUID (CSF)
CEREBROSPINAL FLUID (CSF)CEREBROSPINAL FLUID (CSF)
CEREBROSPINAL FLUID (CSF)
 
Oxidative Stress in Preeclampsia
Oxidative Stress in Preeclampsia Oxidative Stress in Preeclampsia
Oxidative Stress in Preeclampsia
 
GANDHAM RAJEEV-BIOCHEMISTRY IMPORTANT QUESTIONS-RGUHS-2017
GANDHAM RAJEEV-BIOCHEMISTRY IMPORTANT QUESTIONS-RGUHS-2017GANDHAM RAJEEV-BIOCHEMISTRY IMPORTANT QUESTIONS-RGUHS-2017
GANDHAM RAJEEV-BIOCHEMISTRY IMPORTANT QUESTIONS-RGUHS-2017
 
MINERALS-REVISION - 27-05-2017
MINERALS-REVISION - 27-05-2017MINERALS-REVISION - 27-05-2017
MINERALS-REVISION - 27-05-2017
 
IRON METABOLISM
IRON METABOLISMIRON METABOLISM
IRON METABOLISM
 
METABOLISM OF ZINC, MAGNESIUM & ELECTROLYTES
METABOLISM OF ZINC, MAGNESIUM & ELECTROLYTESMETABOLISM OF ZINC, MAGNESIUM & ELECTROLYTES
METABOLISM OF ZINC, MAGNESIUM & ELECTROLYTES
 
METABOLISM OF SULFUR, IODINE, MANGANESE,FLUORINE & SELENIUM
METABOLISM OF SULFUR, IODINE, MANGANESE,FLUORINE & SELENIUMMETABOLISM OF SULFUR, IODINE, MANGANESE,FLUORINE & SELENIUM
METABOLISM OF SULFUR, IODINE, MANGANESE,FLUORINE & SELENIUM
 
COPPER METABOLISM
COPPER METABOLISMCOPPER METABOLISM
COPPER METABOLISM
 
MATABOLISM OF CALCIUM & PHOSPHOROUS
MATABOLISM OF CALCIUM & PHOSPHOROUSMATABOLISM OF CALCIUM & PHOSPHOROUS
MATABOLISM OF CALCIUM & PHOSPHOROUS
 
VITAMIN LIKE COMPOUNDS
VITAMIN LIKE COMPOUNDS VITAMIN LIKE COMPOUNDS
VITAMIN LIKE COMPOUNDS
 
COBALAMINE (12)
COBALAMINE (12) COBALAMINE (12)
COBALAMINE (12)
 
FOLIC ACID (B9)
FOLIC ACID (B9)FOLIC ACID (B9)
FOLIC ACID (B9)
 
PANTOTHENIC ACID (B5)
PANTOTHENIC ACID (B5)PANTOTHENIC ACID (B5)
PANTOTHENIC ACID (B5)
 
VITAMIN K
VITAMIN KVITAMIN K
VITAMIN K
 
VITAMIN D
VITAMIN D VITAMIN D
VITAMIN D
 
VITAMIN E
VITAMIN EVITAMIN E
VITAMIN E
 
Identification of unknown carbohydrate solution
Identification of unknown carbohydrate solutionIdentification of unknown carbohydrate solution
Identification of unknown carbohydrate solution
 

Recently uploaded

Adenomyosis or Fibroid- making right diagnosis
Adenomyosis or Fibroid- making right diagnosisAdenomyosis or Fibroid- making right diagnosis
Adenomyosis or Fibroid- making right diagnosisSujoy Dasgupta
 
blood bank management system project report
blood bank management system project reportblood bank management system project report
blood bank management system project reportNARMADAPETROLEUMGAS
 
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA .pdf
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA    .pdfSGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA    .pdf
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA .pdfHongBiThi1
 
CONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdf
CONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdfCONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdf
CONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdfDolisha Warbi
 
ANATOMICAL FAETURES OF BONES FOR NURSING STUDENTS .pptx
ANATOMICAL FAETURES OF BONES  FOR NURSING STUDENTS .pptxANATOMICAL FAETURES OF BONES  FOR NURSING STUDENTS .pptx
ANATOMICAL FAETURES OF BONES FOR NURSING STUDENTS .pptxWINCY THIRUMURUGAN
 
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdfSGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdfHongBiThi1
 
ORAL HYPOGLYCAEMIC AGENTS - PART 2.pptx
ORAL HYPOGLYCAEMIC AGENTS  - PART 2.pptxORAL HYPOGLYCAEMIC AGENTS  - PART 2.pptx
ORAL HYPOGLYCAEMIC AGENTS - PART 2.pptxNIKITA BHUTE
 
pA2 value, Schild plot and pD2 values- applications in pharmacology
pA2 value, Schild plot and pD2 values- applications in pharmacologypA2 value, Schild plot and pD2 values- applications in pharmacology
pA2 value, Schild plot and pD2 values- applications in pharmacologyDeepakDaniel9
 
How to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturallyHow to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturallyZurück zum Ursprung
 
EXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionEXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionkrishnareddy157915
 
Unit I herbs as raw materials, biodynamic agriculture.ppt
Unit I herbs as raw materials, biodynamic agriculture.pptUnit I herbs as raw materials, biodynamic agriculture.ppt
Unit I herbs as raw materials, biodynamic agriculture.pptPradnya Wadekar
 
Male Infertility Panel Discussion by Dr Sujoy Dasgupta
Male Infertility Panel Discussion by Dr Sujoy DasguptaMale Infertility Panel Discussion by Dr Sujoy Dasgupta
Male Infertility Panel Discussion by Dr Sujoy DasguptaSujoy Dasgupta
 
Red Blood Cells_anemia & polycythemia.pdf
Red Blood Cells_anemia & polycythemia.pdfRed Blood Cells_anemia & polycythemia.pdf
Red Blood Cells_anemia & polycythemia.pdfMedicoseAcademics
 
Bulimia nervosa ( Eating Disorders) Mental Health Nursing.
Bulimia nervosa ( Eating Disorders) Mental Health Nursing.Bulimia nervosa ( Eating Disorders) Mental Health Nursing.
Bulimia nervosa ( Eating Disorders) Mental Health Nursing.aarjukhadka22
 
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdfPAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdfDolisha Warbi
 
AORTIC DISSECTION and management of aortic dissection
AORTIC DISSECTION and management of aortic dissectionAORTIC DISSECTION and management of aortic dissection
AORTIC DISSECTION and management of aortic dissectiondrhanifmohdali
 
Male Infertility, Antioxidants and Beyond
Male Infertility, Antioxidants and BeyondMale Infertility, Antioxidants and Beyond
Male Infertility, Antioxidants and BeyondSujoy Dasgupta
 
MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.whalesdesign
 

Recently uploaded (20)

How to master Steroid (glucocorticoids) prescription, different scenarios, ca...
How to master Steroid (glucocorticoids) prescription, different scenarios, ca...How to master Steroid (glucocorticoids) prescription, different scenarios, ca...
How to master Steroid (glucocorticoids) prescription, different scenarios, ca...
 
Adenomyosis or Fibroid- making right diagnosis
Adenomyosis or Fibroid- making right diagnosisAdenomyosis or Fibroid- making right diagnosis
Adenomyosis or Fibroid- making right diagnosis
 
blood bank management system project report
blood bank management system project reportblood bank management system project report
blood bank management system project report
 
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA .pdf
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA    .pdfSGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA    .pdf
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA .pdf
 
Biologic therapy ice breaking in rheumatology, Case based approach with appli...
Biologic therapy ice breaking in rheumatology, Case based approach with appli...Biologic therapy ice breaking in rheumatology, Case based approach with appli...
Biologic therapy ice breaking in rheumatology, Case based approach with appli...
 
CONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdf
CONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdfCONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdf
CONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdf
 
ANATOMICAL FAETURES OF BONES FOR NURSING STUDENTS .pptx
ANATOMICAL FAETURES OF BONES  FOR NURSING STUDENTS .pptxANATOMICAL FAETURES OF BONES  FOR NURSING STUDENTS .pptx
ANATOMICAL FAETURES OF BONES FOR NURSING STUDENTS .pptx
 
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdfSGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
 
ORAL HYPOGLYCAEMIC AGENTS - PART 2.pptx
ORAL HYPOGLYCAEMIC AGENTS  - PART 2.pptxORAL HYPOGLYCAEMIC AGENTS  - PART 2.pptx
ORAL HYPOGLYCAEMIC AGENTS - PART 2.pptx
 
pA2 value, Schild plot and pD2 values- applications in pharmacology
pA2 value, Schild plot and pD2 values- applications in pharmacologypA2 value, Schild plot and pD2 values- applications in pharmacology
pA2 value, Schild plot and pD2 values- applications in pharmacology
 
How to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturallyHow to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturally
 
EXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionEXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung function
 
Unit I herbs as raw materials, biodynamic agriculture.ppt
Unit I herbs as raw materials, biodynamic agriculture.pptUnit I herbs as raw materials, biodynamic agriculture.ppt
Unit I herbs as raw materials, biodynamic agriculture.ppt
 
Male Infertility Panel Discussion by Dr Sujoy Dasgupta
Male Infertility Panel Discussion by Dr Sujoy DasguptaMale Infertility Panel Discussion by Dr Sujoy Dasgupta
Male Infertility Panel Discussion by Dr Sujoy Dasgupta
 
Red Blood Cells_anemia & polycythemia.pdf
Red Blood Cells_anemia & polycythemia.pdfRed Blood Cells_anemia & polycythemia.pdf
Red Blood Cells_anemia & polycythemia.pdf
 
Bulimia nervosa ( Eating Disorders) Mental Health Nursing.
Bulimia nervosa ( Eating Disorders) Mental Health Nursing.Bulimia nervosa ( Eating Disorders) Mental Health Nursing.
Bulimia nervosa ( Eating Disorders) Mental Health Nursing.
 
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdfPAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
 
AORTIC DISSECTION and management of aortic dissection
AORTIC DISSECTION and management of aortic dissectionAORTIC DISSECTION and management of aortic dissection
AORTIC DISSECTION and management of aortic dissection
 
Male Infertility, Antioxidants and Beyond
Male Infertility, Antioxidants and BeyondMale Infertility, Antioxidants and Beyond
Male Infertility, Antioxidants and Beyond
 
MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.
 

BIOTIN (B7)

  • 1. BIOTIN (B7] Gandham. Rajeev Department of Biochemistry, Akash Institute of Medical Sciences & Research Centre, Devanahalli, Bangalore, Karnataka, India. E-Mail: gandhamrajeev33@gmail.com
  • 4.  Biotin is formerly known as anti-egg white injury factor or vitamin H  It is water soluble sulfur containing B-complex vitamin  Biotin mainly participates in the carboxylation reactions
  • 5.  Biotin (B7) is a heterocyclic sulfur containing monocarboxylic acid  Biotin is imidazole derivative  It is formed by fusion of imidazole & thiophene rings with a valeric acid side chain  Biotin covalently bound to ε – amino group of lysine to form biocytin
  • 6.  Biocytin is the coenzyme form of Biotin  Biotin is a prosthetic group of carboxylase
  • 7.  Biotin is required for carboxylation reactions (CO2 fixation reaction)  Biotin is required for the enzymes  Pyruvate carboxylase  Acetyl CoA carboxylase  Propionyl carboxylase  β - Methyl crotonyl CoA carboxylase
  • 8. Pyruvate carboxylase  Pyruvate carboxylase catalyzes the conversion of pyruvate to oxaloacetate Pyruvate Oxaloacetate CO2, ATP Pyruvate carboxylase BiotinADP+Pi Mg++/Mn
  • 9. Acetyl CoA carboxylase  Acetyl CoA carboxylase catalyzes the formation of malonyl CoA from acetyl CoA  The reaction provides acetate molecule for fattyacid synthesis AcetylCoA Malonyl CoA CO2, ATP Acetyl CoA carboxylase BiotinADP+Pi Mg++/Mn
  • 10. Propionyl CoA Carboxylase  Propionyl CoA carboxylase catalyzes the formation of D – Methyl malonyl CoA from propionyl CoA(from odd chain FA & methionine)  It required for entry of Propionyl CoA to TCA cycle via succinyl CoA Propionyl CoA D – Methyl malonyl CoA CO2, ATP Propionyl CoA carboxylase BiotinADP+PiMg++/Mn
  • 11. β - Methyl crotonyl CoA carboxylase  β - Methyl crotonyl CoA carboxylase catalyzes the formation of β – Methylglutaconyl CoA from β - Methyl crotonyl CoA  It essential for leucine catabolism β - Methyl crotonyl CoA β – Methylglutaconyl CoA CO2, ATP β - Methyl crotonylCoA carboxylase BiotinADP+PiMg++/Mn
  • 12.  All the carboxylation reactions in the biological system are not dependent on Biotin  Few carboxylation reactions which do not require biotin  Formation of carbamoyl phosphate in urea cycle  Incorporation of CO2 in purine synthesis
  • 13.  Rich sources are eggs, liver, kidney, & yeast, pulses, nuts, vegetables  Poor sources are cereals & dairy products RDA Adults - 200 – 300 mg/day
  • 14.  Biotin deficiency is generally not seen in man because of  Its wide distribution in foods  Synthesis of vitamin by the bacterial flora in the gut  Clinical features  Severe dermatitis, weakness, & nausea  In animals muscle weakness, dermatitis & loss of hair around the eye
  • 17.  Avidin ( Raw egg white injury factor )  Raw egg white injury factor is a heat labile protein known as avidin & is present in raw egg white  Avidin binds to biotin & makes its unavailable for absorption  Avidin is inactivated by boiling the eggs & biotin is readily absorbed when boiled eggs are used in the diet
  • 18.  One molecule of avidin can combine with four molecules of biotin  Egg white contains Avidin & egg yolk contains biotin  The affinity of Avidin to biotin is greater than most of the usual antigen-antibody reactions  Avidin-biotin system is commonly utilized for detection of pathogenesis in ELISA test  DNA is generally labelled by radioactive nucleotides
  • 19.  Recently, biotin labelling of DNA is becoming more popular  Biotin is added to nucleotides, which will be incorporated into the newly synthesized DNA  The fixed biotin can be identified by reaction with Avidin  Intake of 20 raw eggs/day will produce Biotin deficiency in humans  Prolonged use of antibacterial drugs such as sulfonamides
  • 20. References  Harper’s Biochemistry 25th Edition.  Fundamentals of Clinical Chemistry by Tietz.  Text Book of Medical Biochemistry-A R Aroor.  Text Book of Biochemistry-DM Vasudevan  Text Book of Biochemistry-MN Chatterjea  Text Book of Biochemistry-Dr.U.Satyanarana