Lactobacillus Pro-t-action is a revolutionary innovation of the German BASF Future Business GmbH company, developed from probiotic cultures of the L. paracasei bacteria. The special characteristic of Pro-t-action is that it reacts only with the cavity-causing bacteria, and it does not affect the number of other bacteria in the mouth. According to the recent studies presented at international conferences, Lactobacillus pro-t-action reduces the number of S. mutans bacteria, the most responsible pathogens in forming cavities, to even up to 50% if used daily. The new toothpaste Plidenta Pro-t-action was developed by the experts in Croatian based company Neva, an Atlantic Group member, as the first toothpaste in the world to contain this probiotic culture. The clinical testing conducted by doc.dr.sc. Dubravka Negovetić Vranić and prof.dr.sc. Martina Majstorović was the first such testing of a toothpaste with the probiotic Lactobacillus paracasei bacteria in the world. The Ministry of Health and Social Welfare of the Republic of Croatia listed Plidenta Pro-t-action toothpaste as a special use cosmetics.

1. PLIDENTA PRO-T-ACTION
Bite down on cavities!
NEW GENERATION ANTI-CAVITY TOOTHPASTES

2. CARIES EPIDEMIOLOGY &
MICROBIOLOGICAL SITUATION
Caries is the most common infectious disease
More than 5 bn. people suffer from tooth decay
Epidemiological studies1 show an alarming global
increase in caries prevalence
S. mutans
Mutan
Caries is very strongly associated with colonization
of the tooth surface biofilm by mutan-producing
streptococci.
Agglutinin
Tooth surface
1: The
Most common: Streptococcus mutans
global increase in dental caries. A pending public health crisis; Am.J.Dent. 22, 2009, 3-8

3. WHAT IS L. PRO-T-ACTION?
Lactobacillus Pro-t-action is a specifically acting
probiotic (Lacobacillus paracasei) which coaggregates mutans Streptococci and thus reduces
caries-causing bacteria in the mouth.
Scanning electron microscope,
4000 x magnification
Lactobacillus Pro-t-action is a trademark of BASF
Future Business GmbH, Germany
It is scent-free and taste-free in the toothpaste, it
does not irritate the mucous membrane and it
contributes to the maintenance of healthy and
useful microflora in the mouth with regular daily
use.
Scanning electron microscope,
21000 x magnification
Plidenta Pro-t-action is the first toothpaste in the
world to contain this probiotic culture.

4. HOW IT WORKS IN TOOTHPASTE?
Acid eats away at tooth
enemal and caause cavities
Caries is very strongly associated with colonization of
the tooth surface biofilm by mutan-producing
streptococci. Most common: Streptococcus mutans
Pro-t-action collects bad bacteria
(S. Mutans)
By teeth washing the bacteria are
flushed out of the mouth
Lactobacillus Pro-t-action selectively gathers and
groups Streptococcus mutans bacteria
Sodium Fluoride strengthens tooth enamel

5. HOW IT LOOKS?
S. mutans
Microscopy: phase contrast and fluorescent labelling
LB pro-t-action®
Aggregates

6. PLIDENTA PRO-T-ACTION
Prevents plaque
Contributes to the removal of the Streptococcus
mutans bacteria, the most common cause of cavities
Creates an environment for the development of
good bacteria
Reacts exclusively with cavity-causing bacteria, but
does not affect other bacteria in the mouth
Acts already after 15 seconds of brushing
According to scientific research presented at
international professional conferences, Lactobacillus
Pro-t-action decreases the number of S. mutans
bacteria, which is considered to be the most
responsible for cavities, by up to 50% with regular
daily use.
Prevents the accumulation of plaque
Strengthens tooth enamel

7. CLINICAL RESEARCH
MARTINA MAJSTOROVIC, Associate Professor,
DUBRAVKA NEGOVETIC VRANIC, Assistant Professor
School of Dental Medicine, University of Zagreb,
Department of Paediatric Dentistry
Efficacy of probiotic toothpaste (Plidenta Pro-taction, Neva, Croatia) related to the presence of
cariogenic bacteria in the oral cavity
There was a statistically significant decrease in the
number of participants with a very high number of
streptococcus (78.4% vs.26.5%) and lactobacillus (52.9%
vs. 26.5%) after 4 weeks of using Plidenta toothpaste. As
to gender, no statisticallly significant difference was
found neither with respect to the number of Sreptococci
and Lactobacilli, or the pH status. Conclusion: Regular
usage of tested toothpaste reduces the number of
cariogenic bacteria in the saliva.

9. REF. JOURNAL PUBLICATIONS
LANG C, BÖTTNER M, HOLZ C, VEEN M, RYSER M,
REINDL A, POMPEJUS M and TANZER JM.
Specific Lactobacillus/Mutans Streptococcus coaggregation. (2010) J Dent Res. 89(2): 175-9
TANZER JM, THOMPSON A, LANG C, COOPER B,
HARENG L, GAMER A, REINDL A, and POMPEJUS M.
Caries inhibition by and safety of Lactobacillus
paracasei DSMZ16671. (2010) J Dent Res. 89(9):
921-6.

10. REF. CONFERENCE PUBLICATIONS
C. LANG, M. BOETTNER, J. TANZER, A. REINDL, and M. POMPEJUS
Lactobacillus paracasei DSM16671 Specifically Co-aggregates with Mutans Streptococci
(2009) J Dent Res 88 (Spec Iss A): 143
J. TANZER, A. THOMPSON, C. LANG, M. BOETTNER, A. REINDL, and M. POMPEJUS
Pasteurized Probiotic Lactobacillus paracasei Inhibits Streptococcus mutans
Colonization, and Caries (2009) J Dent Res 88 (Spec Iss A):144
M. POMPEJUS, B. COOPER, C. LANG, J. TANZER, A. REINDL, and L. HARENG
Streptococcus mutans aggregating Lactobacillus paracasei DSM16671 is safe to use
(2009) J Dent Res 88 (Spec Iss A):1189
M. CHAPAT, M. HANNIG, S. RUPF, M. HENKES and M. POMPEJUS
Influence of heat-killed Lactobacillus paracasei on biofilm formation in situ
(2011) J Dent Res 90 (Spec Iss A): 2659
M. POMPEJUS, C. ALEXANDER, C. HOLZ, C. BALCKE, L. WONG and C. LANG
Reduction of salivary mutans streptococci levels by Lactobacillus paracasei DSMZ16671
(2011) J Dent Res 90 (Spec Iss A): 2762

11. REF. CLINICAL RESEARCH
MARTINA MAJSTOROVIC, Associate Professor,
DUBRAVKA NEGOVETIC VRANIC, Assistant Professor
School of Dental Medicine, University of Zagreb,
Department of Paediatric Dentistry
Efficacy of probiotic toothpaste (Plidenta Pro-taction, Neva, Croatia) related to the presence of
cariogenic bacteria in the oral cavity
Objective: The aim of this study was to evaluate potential benefits from using a probiotic toothpaste (Plidenta Pro-taction, Neva, Croatia) in decreasing the number of cariogenic bacteria (Streptococcus mutans and Lactobacillus)
that are normally present in the mouth. The purpose was also to evaluate a potential influence of toothbrushing on
the saliva buffer capacity. Materials and methods: This research study was conducted on a sample of 50 patients
over a 4 week time frame interval. CRT tests were individually applied to each participant included in the study at
the beginning as well as at the 2 and 4 week intervals during which the patients were instructed to brush their teeth
by using Plidenta toothpaste. The number of Streptococcus mutans and Lactobacillus was taken from saliva and
counted after 48 hours. Buffer capacity of saliva was measured for each patient at the 2 and 4 week check-ups.
Patients were provided with the toothbrushes of medium hardness and were instructed to brush their teeth
thouroughly 2 times a day by using Plidenta probiotic toothpaste. Patients were in the age group between 20 and
24 years of age equally divided by genders. Results:There was a statistically significant decrease in the number of
participants with a very high number of streptococcus (78.4% vs.26.5%) and lactobacillus (52.9% vs. 26.5%) after 4
weeks of using Plidenta toothpaste. As to gender, no statisticallly significant difference was found neither with
respect to the number of Sreptococci and Lactobacilli, or the pH status. Conclusion: Regular usage of tested
toothpaste reduces the number of cariogenic bacteria in the saliva.

12. M. CHAPAT, M. HANNIG, S. RUPF, M. HENKES and M.
POMPEJUS
Influence of heat-killed Lactobacillus paracasei on
biofilm formation in situ
(2011) J Dent Res 90 (Spec Iss A): 2659
Objectives: Mutans streptococci are important cariogenic pathogens. Lactobacillus paracasei DSMZ16671
specifically co-aggregates with mutans streptococci. The aim of this in situ study was to investigate 1) the adherence
of heat-killed L. paracasei DSMZ16671 on pellicle covered enamel surfaces, 2) their adherence on matured biofilms
and, 3) the influence on the amount of Streptococcus mutans in saliva. Methods: Enamel slices of bovine incisors
(5x5x2 mm) were exposed intraorally (6 participants) for 3 min up to 48 h for pellicle and for biofilm formation.
Participants rinsed with L. paracasei mouthrinse (107/ml) or placebo mouthrinse for 30 s every 12 h. Paraffin
stimulated saliva samples were taken after 24 h and after 48 h before rinsing. The vitality of the biofilms was
compared by live/dead staining after rinsing with L. paracasei mouthrinse or placebo mouthrinse. Biofilm
morphology was visualized by scanning-electron-microscopy (SEM) assessing the occurrence of rod-shaped
bacteria. Saliva samples were plated on MSB agar with bacitracin and mutans streptococci amounts were
determined. All experiments were carried out in triplicates. Results: Biofilm amounts and viability of biofilms were
comparable for L. paracasei mouthrinse and placebo mouthrinse. However, in SEM the ratio of rod-shaped bacteria
was slightly increased in biofilms exposed to L. paracasei mouthrinse. The amount of mutans streptococci in saliva
samples was reduced by 50 percent after using L. paracasei mouthrinse. Conclusions: The results indicate reduction
of salivary levels of mutans streptococci and no adverse effects in dental biofilms under the influence of
mouthrinses with heat-killed L. paracasei. The application of this heat killed probiotic strain could enable new
strategies for caries control by reduction of mutans streptococci in the oral cavity

13. M. POMPEJUS, C. ALEXANDER, C. HOLZ, C. BALCKE, L.
WONG and C. LANG
Reduction of salivary mutans streptococci levels by
Lactobacillus paracasei DSMZ16671 (2011) J Dent Res 90
(Spec Iss A): 2762
Objectives: One important strategy to prevent tooth decay can be the reduction of mutans streptococci levels in the
oral cavity. Lactobacillus paracasei DSMZ16671 has been shown to specifically co-aggregate mutans streptococci invitro and to inhibit caries in rats. Purpose of this study is to evaluate the functionality of L. paracasei DSMZ16671 in
the oral cavity of healthy humans using sugar-free candies as delivery vehicle. Methods: A double-blind,
randomized, placebo-controlled clinical trial with 60 healthy adults was set-up. 2 different concentrations of L.
paracasei DSMZ16671 or placebo were delivered in sugar-free hard candies. Saliva samples were collected
immediately before and after consumption of a single sugar-free candy. Mutans streptococci levels were
determined by cultivation on selective growth media. Results: The group consuming placebo candies showed no
changes in the salivary level of mutans streptococci. In contrast, both test groups consuming candies containing L.
paracasei DSMZ16671 showed reduction of the salivary level of mutans streptococci in a dose-dependent manner.
Conclusions: The data provide evidence that L. paracasei DSMZ16671 delivered in sugar-free candies is effectively
reducing salivary mutans streptococci levels. Use of L. paracasei DSMZ16671 in sugar-free candies is suggested as an
attractive way of daily oral hygiene.

14. CREDENTIALS
Prof. Dr. Ch. Lang - Presentations on the 8th AsiaPacific Weeks (APW) in Berlin

15. CREDENTIALS
Datamonitor Product Launch Analitics by Symrise
Quarterly Report, New Launch Alert , July 2011

16. CREDENTIALS
Declaration of Ministry of Health and Welfare of the
Republic of Croatian as COSMETIC WITH SPECIAL
EFFECTS (PURPOSES)
“HELPS TO PREVENT TOOTH DECAY CAUSED BY
BACTERIA S- MUTANS AND THE CREATION OF
DENTAL PLAQUE AS WELL AS STRENGHTENES OF
TOOTH ENAMEL”

17. CREDENTIALS
Declaration of Ministry of Health B&H as COSMETIC
WITH SPECIAL EFFECTS (PURPOSES)
“HELPS TO PREVENT TOOTH DECAY CAUSED BY
BACTERIA S- MUTANS AND THE CREATION OF
DENTAL PLAQUE AS WELL AS STRENGHTENES OF
TOOTH ENAMEL”

18. CREDENTIALS
TECHNOLOGY REVIEW

19. CREDENTIALS
Martina Majstorovic, Associate Professor, Dubravka Negovetic Vranic, Assistant Professor
School of Dental Medicine, University of Zagreb, Department of Paediatric Dentistry, Authors Plidenta Pro-t-action
Clinical Study
“The clinical research results conducted on this sample have suggested that regular daily use of Plidenta’s new
toothpaste with probiotic bacteria, whose mechanism is based on selective removal of cariogenic
bacteria, contributes to reducing the risk of caries. So this toothpaste represents the most innovative product
currently available on our toothpaste market, whose regular use can help achieve a significant effect in the
prevention of cavities. It acts as an excellent therapeutic medium which leads to the optimal ratio of good and bad
bacteria in the saliva which represents a significant innovation in preventive dentistry, especially in patients at high
risk for cavities and with a large number of potentially pathogenic bacteria in their mouths,”
Nutraingredients.com http://www.nutraingredients.com/content/view/print/317675
Tooth-friendly probiotic products are on horizon, BASF “… Dr Christine Lang from OrganoBalance told delegates at the
International Probiotics Association's (IPA) congress in Miami this year that the concept of probiotics for oral health
centred on S.mutans as the main bacteria associated with tooth decay. Lang explained that the bacteria bind to teeth
forming dental plaque, which then converts sugar into acid that attacks tooth enamel. Lang and her team screened
over 700 Lactobacillus strains, and found that Lactobacillus paracasei aggregated best with S.mutans, clumping
bacteria together and allowing easy removal from the mouth via swallowing or rinsing.

20. CREDENTIALS
Prof. Dr. Jagoda Šuškovid, Food Technology and Biotechnology, University of Zagreb, Laboratory for
antibiotics, enzymes, probiotics and starter cultures
“One way of combating cavities and application of probiotic strains that can interfere with the bacterium
Streptococcus mutans adhesion prevention and the creation of dental plaque biofilms. Based on this mechanism of
action has recently marketed toothpaste Plidenta Pro-t-action containing the probiotic bacterium effective to the
pathogenic bacterium Streptococcus mutans. This product is a result of the world's scientific knowledge (company
BASF - Future Business GmbH, Deutschland) and our local knowledge (of Neva and the Laboratory for
antibiotics, enzymes, probiotics and starter cultures, Food Technology and Biotechnology, University of Zagreb).”
Prof. Dr. J. Tanzer, The University of Connecticut Health Center
“The laboratory tests conducted at the University of Connecticut Health Center demonstrated the ability of the
L. paracasei used in pro-t-action™ to significantly reduce the levels of decay. “These findings, which are the logical
consequence of the ability of selected L. paracasei to specifically clump Streptococcus mutans, present an intriguing
new approach to controlling this miserable, prevalent, and costly disease, tooth decay.”

21. CONTACT
Neva Marketing
Atlantic Grupa
HealthCare Division
Miramarska 23
10000 Zagreb
Croatia
E-mail: branimir.zoraja@atlanticgrupa.com
Phone: + 385 91 2413 731
www.plidenta.com/en
www.facebook.com/plidenta.hr
www.youtube.com/ThePlidenta