Hiv diagnosis

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OmEr HaXhme

how to diagnose hiv in elderly patient.. stepwise guide,, very precise

Health & Medicine

HOW TO DETECT
•

HIV

SCREENING TESTS
CONFIRMATORY TESTS

•
•

MOLECULAR TESTS

SCREENING TESTS
Rapid kit test
ELISA (enzyme linked immunosorbant essay)
• Most reliable,

has 99.9 % sensitivity

• Third and fourth generations are being used, detect all antibodies (IgM)

CONFIRMATORY TESTS
WESTERN blot
Specificity 99.9%

• Line immunoassay (LIA)
• Immunofluorescence assay (IFA)

• MOLECULAR MARKERS
•

Polymerase chain reaction (PCR) RNA
Nucliec acid sequence amplification (NASBA)

•

•

P24 CORE ANTIGEN

• CD4 CELLS
•

Risk of infection increses when count less then 200*10^6/L

•

Help staging disease and monitring therapy

CONTROL PROGRAM | HEALTH DEPARTMENTHIV AND AIDS: DIAGNOSIS & TESTS
HEALTH.PUNJAB.GOV.PK

OXFOR HANDBOOK OF CLINICAL MEDICINE EIGHTH EDITION . MURAY LORANG. PAGE 408

en.wikipedia.org

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Hiv diagnosis

1. DIAGNOSIS • OMER BASHIR HASHMI

2. HOW TO DETECT • HIV SCREENING TESTS CONFIRMATORY TESTS • • MOLECULAR TESTS

3. SCREENING TESTS Rapid kit test ELISA (enzyme linked immunosorbant essay) • Most reliable, has 99.9 % sensitivity • Third and fourth generations are being used, detect all antibodies (IgM) CONFIRMATORY TESTS WESTERN blot Specificity 99.9%

4. • Line immunoassay (LIA) • Immunofluorescence assay (IFA) • MOLECULAR MARKERS • Polymerase chain reaction (PCR) RNA Nucliec acid sequence amplification (NASBA) • • P24 CORE ANTIGEN • CD4 CELLS • Risk of infection increses when count less then 200*10^6/L • Help staging disease and monitring therapy

5. CONTROL PROGRAM | HEALTH DEPARTMENTHIV AND AIDS: DIAGNOSIS & TESTS HEALTH.PUNJAB.GOV.PK OXFOR HANDBOOK OF CLINICAL MEDICINE EIGHTH EDITION . MURAY LORANG. PAGE 408 en.wikipedia.org

Hiv diagnosis

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