The document discusses vitamin K2 and its role in bone and heart health. It provides information on osteoporosis prevalence and fractures. It explains how vitamin K2 activates proteins involved in calcium binding and bone mineralization, as well as inhibits arterial calcification. Data is presented showing risks of cardiovascular events and mortality are reduced with higher vitamin K2 intake. Sources of K1 and different forms of K2 are outlined, with MK-7 highlighted as having the best bioavailability and potential for protein activation. Quality aspects of MK-7 supplements are also covered.

1. Vitamin K2MK7
connecting bone & heart health
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2. 1/3 women over 50
will experience osteoporotic fractures
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3. 3 million UK
residentes with osteoporosis
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4. 190,000
osteoporosis-related fractures
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5. Calcium
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6. © ppm.Ingredients KG, 2014

7. Calcium in the body
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8. Calcium in the body
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9. Do you know
Vitamin K2 ?
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10. © ppm.Ingredients KG, 2014

11. How K2 works
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12. Ca
Ca
Ca
Ca
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13. Osteoblast
Osteoclast
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14. Osteoblast
under-carboxylated
Osteocalcin
(inactive)
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15. Osteoblast
carboxylation
(activation of OC)
C
C C
Gamma-glutamyl carboxylase
+ Vitamin K2
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16. Ca
carboxylatied OC
binds Calcium
Ca
Ca
C
C
C
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17. Osteoblast
Osteoclast
Ca
Ca
Ca
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18. ucOC
(inactive)
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
Ca
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19. Bone Health
Vitamin K2
Uptake of Ca2+
Production of OC
Vit D
Uptake of Ca2+
Intestine
Ca2+
Activation of OC
Vit K2
Bone mineralisation
cOC
Ca2+
Ca2+
Ca2+
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20. e risk of cardiovascular events is caused by missing
Vitamin K – not by Calcium supplementation
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21. Titel
The
Secrets
of
Ac1vator
X
K2
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22. Matrix Gla-protein (MGP)
Calcium ions binding to activated MGP
MGP is an important inhibitor of calcification in soft tissue
carboxylated MGP
(active)
Ca
Ca
Ca
Ca
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23. Activated MGP reduces
arterial calcification
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24. 0
0,2
0,4
0,6
0,8
1
1,2
K2<21
K2 21-32
K2>32
Severe calcification
Incidence of CHD
CHD mortality
All cause mortality
e Rotterdam study
RelativeRiskHazardRatio
4807 Dutch people followed 10 years
Dietary vitamin K2 intake (µg/day)
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25. Titel
The
Secrets
of
Ac1vator
X
K2
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26. Where do we get
Vitamin K from?
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27. Vitamin K1
Phylloquinone
• Made by plants and algae
• Bound to chloroplasts
• Only 5-10% reaches circulation
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28. Vitamin K2 MK-4
Menaquinone-4
• Synthetic feed grade
• Pharmacokinetics like K1
• Drug in Japan: 45 mg/day
• Several older studies
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29. Vitamin K2 MK-7
Menaquinone-7
• Made by Bacillus subtilis natto
• Found in certain fermented foods
• Nearly 100% absorbable
• Not available in Western diet
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30. Vitamin K co-factor for
Activation of Gla-proteins
Clotting factors
activated by K1 and K2
Osteocalcin
ativated by K1 and K2
Matrix Gla-protein
activated mainly by K2
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31. Bioavailability K1 vs K2 MK-7
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32. Vitamin K2 MK-7
Menaquinone-7
• Best absorption
• Longest half-life
• Non toxic
• Highest overall potential for Gla activation
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33. Quality Aspects
VitaminK2
Menaquinone-7
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34. 1 – 2 – 3 – 4 – 5 – 6 – 7
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35. cis isomer is bio-inactive!
trans bond
cis bond
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36. stable in tablets and alkali environment
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37. and other allergens and free of residual solvents
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38. and reference standards available
DETERMINATION OF MK-7 (VITAMIN I(r) IN K2VITAL MCT-OtrL
Synthetica AS A_HPLC_SYN_OO6.03 Page I of5
1. INTRODUCTION
This method is used for assay determination of MK-7(vitamin K2) in K2VITAL MCT- OIL
product and quantification of impurities in the product. The MCT-oil product is diluted with
ethyl acetate and the amount of MK-7 is determined by HPLC with UV detection.
2. ABBREVIATIONS
MK.7:
CV:
DAD:
HPLC:
nm:
RSD:
RT:
RRT:
SD:
UV:
pL:
mL:
pm:
w/w:
vlv:
EtOAc:
Synthetic vitamin K2, menaquinone-7
Coeffi cient of Variation
Diode Array Detector
High Performance Liquid Chromatography
Nanometer
Relative Standard Deviation
Retention Time
Relative Retention Time
Standard Deviation
Ultraviolet
Microliter
Milliliter
Micron (Micrometer)
weight/weight
volume/volume
Ethyl acetate
3. APPARATUS
HPLC-DAD high pressure system: Agilent, LC system I100 series (or equivalent).
Analyical balance (Uncertainty + 0.05 mg) (or equivalent).
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39. © ppm.Ingredients KG, 2014

40. © ppm.Ingredients KG, 2014
Manufacturer
Kappa Bioscience AS
www.k2vital.com
www.kappabio.com
Sales & Distribution
ppm.Ingredients KG
Jörg Büttinghaus
k2vital@ppm-ingredients.de
++49-40-6094087-0