SlideShare a Scribd company logo
1 of 15
Download to read offline
PRESENTED BY: 
STUTI TRIPATHI 
M.PHARM 
2nd SEM.
Cellular carriers ppt
Resealed erythrocytes are prepared by collecting blood 
samples from the organism of interest and separating 
erythrocytes from plasma. 
Resealed erythrocytes are biocompatible, 
biodegradable, having long circulation half-life and can 
be loaded with variety of active substances.
By using various methods (discuss later),cells are broken. 
Drug is entrapped into erythrocytes, and then they are 
resealed. 
Resultant carriers are termed as “Resealed erythrocytes”.
PROPERTIES OF RESEALED 
ERYTHROCYTES: 
The drug should be released at target site in a controlled manner. 
It should be appropriate size, shape and should permit the 
passage through capillaries and minimum leakage of drug should 
take place. 
It should be biocompatible and have minimum toxic effect. 
The carrier system should have an appreciable stability during 
storage. 
It should have the ability to carry a broad spectrum of drug. 
The degradation product of the carrier system, after release of the 
drug at selected site should be biocomaptible.
HYPO-OSMOTIC LYSIS METHOD: 
Hypotonic lysis of cells in a solution containing the drug 
to be entrapped followed by restoration of tonicity and 
reseal them. 
The ghost population so obtained are heterogeneous. 
Three types of ghosts can be distinguished : 
a. Type 1:ghosts which reseal immediately after 
haemolysis, 
b. Type 2:ghosts which reseal after reversal of haemolysis 
by addition of alkali ions, and 
c. Type 3:ghosts which remain leaky under different 
experimental conditions.
Sr. 
No. 
Species Washing buffer Centrifugal 
force(g) 
1. Rabbit 10mmol Phosphate buffer 500-1000 
2. Dog 15mmol Phosphate buffer 500-1000 
3. Human 154mmol NaCl <500 
4. Cow 10-15mmol Phosphate buffer 1000 
5. Mouse 10mmol Phosphate buffer 100-500 
6. Horse 2mmol Magnesium 
chloride,10mmol glucose 
1000 
7. Sheep 10mmol Phosphate buffer 500-1000 
8. Pig 10mmol Phosphate buffer 500-1000
CHEMICAL PERTURBATION OF THE 
MEMBRANE: 
This method is based on the increase in membrane 
permeability of erythrocytes when the cells are 
exposed to certain chemicals. 
This method was used successfully by Kitao and 
Hattori to entrap the antineoplastic drug “daunomycin” 
in human and mouse erythrocytes.
ELECTRO-INSERTION OR 
ELECTROCAPSULATION: 
This method is also known as electroporation, based 
on the observation that electrical shock brings about 
irreversible changes in an erythrocyte membrane. 
The erythrocyte membrane is opened by a dielectric 
breakdown. Subsequently, the pores can be resealed 
by incubation at 37⁰c in an isotonic medium.
CHARACTERIZATION OF RESEALED 
ERYTHROCYTES: 
DRUG-CONTENT DETERMINATION: Packed loaded 
cells are deprotenized with acetonitrile after 
centrifugation at 300 rpm for a fixed time interval. The 
clear supernatant liquid is assayed for drug content. 
IN-VITRO DRUG RELEASE AND Hb CONTENT: The 
cell suspensions are stored at 4⁰c in ambered colored 
glass container. Periodically clear supernatant are 
drawn using a hypodermic syringe, filter and 
deproteined with methanol and then estimated for drug 
content. 
%Hb release= A540 of sample – A540 of background
% CELL RECOVERY: May be determined by counting 
the no. of intact cells per cubic mm of packed 
erythrocytes before and after loading the drug. 
ERYTHROCYTE SEDIMENTATION RATE: It is an 
estimate of the suspension stability of RBC in plasma 
and is related to the no. and size of the red cells and to 
relative concentration of plasma protein, especially 
fibrinogen and alpha and beta globulins. This test is 
performed by determining the rate of sedimentation of 
blood cells in a standard tube. 
NORMAL BLOOD ESR=0-15 mm/hr.
APPLICATIONS: 
Targeting of bioactive agents to RE system 
Targeting to sites other than RES-rich organs 
Erythrocytes as circulating bioreactors 
Erythrocytes as carriers for enzymes
APPLICATION DRUG/ENZYME/MACROMOLECULE 
Enzyme deficiency replacement 
therapy 
ẞ- galactosidase , ẞ- 
fructofuronodase,urease,glucose-6- 
phosphatedehydrogenase 
Thrombolytic activity Brinase , aspirin , heparin 
Iron overload Desferroxamine 
Immunotherapy Human recombinant interlukin-2
REFERNCES 
Jain S.K. and Vyas S.P., “Magnetically Responsive 
Diclofenac Sodium- Loaded Erythrocytes: Preparation 
and In-Vitro Characterization, CBS Publishers and 
Distributors, pg. no.-141-51. 
Vyas S.P. and Dixit V.K., Pharmaceutical Biotechnology 
, CBS Publishers & Distributors, New Delhi, pg. no. 655
THANK YOU

More Related Content

What's hot

RESEALED ERYTHROCYTES
RESEALED ERYTHROCYTESRESEALED ERYTHROCYTES
RESEALED ERYTHROCYTESSuresh Rewar
 
Resealed erythrocytes as a novel delivery carrier
Resealed erythrocytes as a novel  delivery carrierResealed erythrocytes as a novel  delivery carrier
Resealed erythrocytes as a novel delivery carrierMariamZewail
 
resealed erythrocytes
resealed erythrocytes resealed erythrocytes
resealed erythrocytes Sonali Pimple
 
Resealed erythrocyte
Resealed erythrocyteResealed erythrocyte
Resealed erythrocytesuhasini
 
Evaluation n application of resealed erythrocytes
Evaluation n application of resealed erythrocytesEvaluation n application of resealed erythrocytes
Evaluation n application of resealed erythrocytesbiniyapatel
 
RESEALED ERYTHROCYTES AS DRUG DELIVERY SYSTEM
RESEALED ERYTHROCYTES AS DRUG DELIVERY SYSTEMRESEALED ERYTHROCYTES AS DRUG DELIVERY SYSTEM
RESEALED ERYTHROCYTES AS DRUG DELIVERY SYSTEMVikas Soni
 
Resealed erythrocytes
Resealed erythrocytes Resealed erythrocytes
Resealed erythrocytes Sagar Savale
 
Resealed erythrocytes in drug delivery
Resealed erythrocytes in drug deliveryResealed erythrocytes in drug delivery
Resealed erythrocytes in drug deliveryBiplajit Das
 
Resealed erythrocytes
Resealed erythrocytes Resealed erythrocytes
Resealed erythrocytes DasaraThanmayi
 
Resealed erythrocytes
Resealed erythrocytesResealed erythrocytes
Resealed erythrocytesVinay Kumar
 
Resealed erythrocytes as a novel delivery carrier
Resealed erythrocytes as a novel  delivery carrierResealed erythrocytes as a novel  delivery carrier
Resealed erythrocytes as a novel delivery carrierMariamZewail
 
Presentation1 riya pfd 1q
Presentation1 riya pfd 1qPresentation1 riya pfd 1q
Presentation1 riya pfd 1qRIYA PATEL
 
resealed erythrocytes
resealed erythrocytes resealed erythrocytes
resealed erythrocytes bss3118
 
Targeted drug delivery system
Targeted drug delivery systemTargeted drug delivery system
Targeted drug delivery systemJyotsana Bhatt
 

What's hot (20)

RESEALED ERYTHROCYTES
RESEALED ERYTHROCYTESRESEALED ERYTHROCYTES
RESEALED ERYTHROCYTES
 
reseald erythrocyte
reseald erythrocytereseald erythrocyte
reseald erythrocyte
 
Resealed erythrocytes
Resealed erythrocytesResealed erythrocytes
Resealed erythrocytes
 
Resealed erythrocytes as a novel delivery carrier
Resealed erythrocytes as a novel  delivery carrierResealed erythrocytes as a novel  delivery carrier
Resealed erythrocytes as a novel delivery carrier
 
resealed erythrocytes
resealed erythrocytes resealed erythrocytes
resealed erythrocytes
 
Resealed erythrocyte
Resealed erythrocyteResealed erythrocyte
Resealed erythrocyte
 
Resealed Erythrocytes
Resealed ErythrocytesResealed Erythrocytes
Resealed Erythrocytes
 
Evaluation n application of resealed erythrocytes
Evaluation n application of resealed erythrocytesEvaluation n application of resealed erythrocytes
Evaluation n application of resealed erythrocytes
 
RESEALED ERYTHROCYTES AS DRUG DELIVERY SYSTEM
RESEALED ERYTHROCYTES AS DRUG DELIVERY SYSTEMRESEALED ERYTHROCYTES AS DRUG DELIVERY SYSTEM
RESEALED ERYTHROCYTES AS DRUG DELIVERY SYSTEM
 
Resealed erythrocytes
Resealed erythrocytes Resealed erythrocytes
Resealed erythrocytes
 
Resealed erythrocytes in drug delivery
Resealed erythrocytes in drug deliveryResealed erythrocytes in drug delivery
Resealed erythrocytes in drug delivery
 
Resealed erythrocytes
Resealed erythrocytes Resealed erythrocytes
Resealed erythrocytes
 
Resealed erythrocytes
Resealed erythrocytesResealed erythrocytes
Resealed erythrocytes
 
Resealed erythrocytes as a novel delivery carrier
Resealed erythrocytes as a novel  delivery carrierResealed erythrocytes as a novel  delivery carrier
Resealed erythrocytes as a novel delivery carrier
 
Resealed erythrocyte- sunil kokate
Resealed  erythrocyte- sunil kokateResealed  erythrocyte- sunil kokate
Resealed erythrocyte- sunil kokate
 
Presentation1 riya pfd 1q
Presentation1 riya pfd 1qPresentation1 riya pfd 1q
Presentation1 riya pfd 1q
 
Resealed erythrocytes
Resealed erythrocytesResealed erythrocytes
Resealed erythrocytes
 
Nano particles and released erythrocytes
Nano particles and released erythrocytesNano particles and released erythrocytes
Nano particles and released erythrocytes
 
resealed erythrocytes
resealed erythrocytes resealed erythrocytes
resealed erythrocytes
 
Targeted drug delivery system
Targeted drug delivery systemTargeted drug delivery system
Targeted drug delivery system
 

Viewers also liked

Cellular network presentation
Cellular network presentationCellular network presentation
Cellular network presentationAditya Pandey
 
Introduction To Cellular Networks
Introduction To Cellular NetworksIntroduction To Cellular Networks
Introduction To Cellular NetworksYoram Orzach
 
Presentation on 1G/2G/3G/4G/5G/Cellular & Wireless Technologies
Presentation on 1G/2G/3G/4G/5G/Cellular & Wireless TechnologiesPresentation on 1G/2G/3G/4G/5G/Cellular & Wireless Technologies
Presentation on 1G/2G/3G/4G/5G/Cellular & Wireless TechnologiesKaushal Kaith
 

Viewers also liked (6)

Cellular network presentation
Cellular network presentationCellular network presentation
Cellular network presentation
 
Resealed erythrocyte-sunil kokate
Resealed  erythrocyte-sunil kokateResealed  erythrocyte-sunil kokate
Resealed erythrocyte-sunil kokate
 
Digital in 2016 Executive Summary
Digital in 2016 Executive SummaryDigital in 2016 Executive Summary
Digital in 2016 Executive Summary
 
Introduction To Cellular Networks
Introduction To Cellular NetworksIntroduction To Cellular Networks
Introduction To Cellular Networks
 
Idea ppt
Idea pptIdea ppt
Idea ppt
 
Presentation on 1G/2G/3G/4G/5G/Cellular & Wireless Technologies
Presentation on 1G/2G/3G/4G/5G/Cellular & Wireless TechnologiesPresentation on 1G/2G/3G/4G/5G/Cellular & Wireless Technologies
Presentation on 1G/2G/3G/4G/5G/Cellular & Wireless Technologies
 

Similar to Cellular carriers ppt

Erythrocytes2 090814024428-phpapp02
Erythrocytes2 090814024428-phpapp02Erythrocytes2 090814024428-phpapp02
Erythrocytes2 090814024428-phpapp02Ashish Garg
 
Encapsulated erythrocytes
Encapsulated erythrocytesEncapsulated erythrocytes
Encapsulated erythrocytesSujeetSingh212
 
Jeevani ( Role of clinical biochemistry in laboratory)
Jeevani ( Role of clinical biochemistry in laboratory)Jeevani ( Role of clinical biochemistry in laboratory)
Jeevani ( Role of clinical biochemistry in laboratory)Potu Jeevani
 
resealederythrocytes as drug carrier .pptx
resealederythrocytes as drug carrier .pptxresealederythrocytes as drug carrier .pptx
resealederythrocytes as drug carrier .pptxHarshadaa bafna
 
In-vitro and in-vivo methods of diuretics & antihypertensive final.pptx
In-vitro and in-vivo methods of diuretics & antihypertensive final.pptxIn-vitro and in-vivo methods of diuretics & antihypertensive final.pptx
In-vitro and in-vivo methods of diuretics & antihypertensive final.pptxAishwaryaPatil697206
 
Industrial production of recombinant Erythropoietin
Industrial production of recombinant Erythropoietin Industrial production of recombinant Erythropoietin
Industrial production of recombinant Erythropoietin ShreyasGowda87
 
The Importance Of Animal Uses In Animals
The Importance Of Animal Uses In AnimalsThe Importance Of Animal Uses In Animals
The Importance Of Animal Uses In AnimalsJessica Lopez
 
ANTIHYPERLIPIDEMIC screening models
ANTIHYPERLIPIDEMIC screening modelsANTIHYPERLIPIDEMIC screening models
ANTIHYPERLIPIDEMIC screening modelsAnmolkanda06
 
methods of determining drug absorption ppt
methods of determining drug absorption pptmethods of determining drug absorption ppt
methods of determining drug absorption ppttenzin jangchuk
 
drug product performance invivo
 drug product performance invivo drug product performance invivo
drug product performance invivoSUJITHA MARY
 
Liposomal Drug Delivery
Liposomal Drug DeliveryLiposomal Drug Delivery
Liposomal Drug DeliveryAlexa Jacob
 
Invitro invivo insitu method
Invitro invivo insitu methodInvitro invivo insitu method
Invitro invivo insitu methodGURUPRASAD S
 

Similar to Cellular carriers ppt (16)

Erythrocytes2 090814024428-phpapp02
Erythrocytes2 090814024428-phpapp02Erythrocytes2 090814024428-phpapp02
Erythrocytes2 090814024428-phpapp02
 
Encapsulated erythrocytes
Encapsulated erythrocytesEncapsulated erythrocytes
Encapsulated erythrocytes
 
Erythrocytes
ErythrocytesErythrocytes
Erythrocytes
 
Ezhil1
Ezhil1Ezhil1
Ezhil1
 
Jeevani ( Role of clinical biochemistry in laboratory)
Jeevani ( Role of clinical biochemistry in laboratory)Jeevani ( Role of clinical biochemistry in laboratory)
Jeevani ( Role of clinical biochemistry in laboratory)
 
resealederythrocytes as drug carrier .pptx
resealederythrocytes as drug carrier .pptxresealederythrocytes as drug carrier .pptx
resealederythrocytes as drug carrier .pptx
 
In-vitro and in-vivo methods of diuretics & antihypertensive final.pptx
In-vitro and in-vivo methods of diuretics & antihypertensive final.pptxIn-vitro and in-vivo methods of diuretics & antihypertensive final.pptx
In-vitro and in-vivo methods of diuretics & antihypertensive final.pptx
 
Industrial production of recombinant Erythropoietin
Industrial production of recombinant Erythropoietin Industrial production of recombinant Erythropoietin
Industrial production of recombinant Erythropoietin
 
Ezhil2.Ppt
Ezhil2.PptEzhil2.Ppt
Ezhil2.Ppt
 
The Importance Of Animal Uses In Animals
The Importance Of Animal Uses In AnimalsThe Importance Of Animal Uses In Animals
The Importance Of Animal Uses In Animals
 
ANTIHYPERLIPIDEMIC screening models
ANTIHYPERLIPIDEMIC screening modelsANTIHYPERLIPIDEMIC screening models
ANTIHYPERLIPIDEMIC screening models
 
methods of determining drug absorption ppt
methods of determining drug absorption pptmethods of determining drug absorption ppt
methods of determining drug absorption ppt
 
drug product performance invivo
 drug product performance invivo drug product performance invivo
drug product performance invivo
 
Liposomal Drug Delivery
Liposomal Drug DeliveryLiposomal Drug Delivery
Liposomal Drug Delivery
 
Infliximab
InfliximabInfliximab
Infliximab
 
Invitro invivo insitu method
Invitro invivo insitu methodInvitro invivo insitu method
Invitro invivo insitu method
 

Recently uploaded

Red Blood Cells_anemia & polycythemia.pdf
Red Blood Cells_anemia & polycythemia.pdfRed Blood Cells_anemia & polycythemia.pdf
Red Blood Cells_anemia & polycythemia.pdfMedicoseAcademics
 
Role of Soap based and synthetic or syndets bar
Role of  Soap based and synthetic or syndets barRole of  Soap based and synthetic or syndets bar
Role of Soap based and synthetic or syndets barmohitRahangdale
 
CPR.nursingoutlook.pdf , Bsc nursing student
CPR.nursingoutlook.pdf , Bsc nursing studentCPR.nursingoutlook.pdf , Bsc nursing student
CPR.nursingoutlook.pdf , Bsc nursing studentsaileshpanda05
 
Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024EwoutSteyerberg1
 
ayurvedic formulations herbal drug technologyppt
ayurvedic formulations herbal drug technologypptayurvedic formulations herbal drug technologyppt
ayurvedic formulations herbal drug technologypptPradnya Wadekar
 
AUTONOMIC NERVOUS SYSTEM organization and functions
AUTONOMIC NERVOUS SYSTEM organization and functionsAUTONOMIC NERVOUS SYSTEM organization and functions
AUTONOMIC NERVOUS SYSTEM organization and functionsMedicoseAcademics
 
Male Infertility Panel Discussion by Dr Sujoy Dasgupta
Male Infertility Panel Discussion by Dr Sujoy DasguptaMale Infertility Panel Discussion by Dr Sujoy Dasgupta
Male Infertility Panel Discussion by Dr Sujoy DasguptaSujoy Dasgupta
 
SGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdf
SGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdfSGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdf
SGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdfHongBiThi1
 
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdfPAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdfDolisha Warbi
 
Basic structure of hair and hair growth cycle.pptx
Basic structure of hair and hair growth cycle.pptxBasic structure of hair and hair growth cycle.pptx
Basic structure of hair and hair growth cycle.pptxkomalt2001
 
historyofpsychiatryinindia. Senthil Thirusangu
historyofpsychiatryinindia. Senthil Thirusanguhistoryofpsychiatryinindia. Senthil Thirusangu
historyofpsychiatryinindia. Senthil Thirusangu Medical University
 
Mental health Team. Dr Senthil Thirusangu
Mental health Team. Dr Senthil ThirusanguMental health Team. Dr Senthil Thirusangu
Mental health Team. Dr Senthil Thirusangu Medical University
 
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptxDNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptxMAsifAhmad
 
EXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionEXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionkrishnareddy157915
 
power point presentation of Clinical evaluation of strabismus
power point presentation of Clinical evaluation  of strabismuspower point presentation of Clinical evaluation  of strabismus
power point presentation of Clinical evaluation of strabismusChandrasekar Reddy
 

Recently uploaded (20)

Immune labs basics part 1 acute phase reactants ESR, CRP Ahmed Yehia Ismaeel,...
Immune labs basics part 1 acute phase reactants ESR, CRP Ahmed Yehia Ismaeel,...Immune labs basics part 1 acute phase reactants ESR, CRP Ahmed Yehia Ismaeel,...
Immune labs basics part 1 acute phase reactants ESR, CRP Ahmed Yehia Ismaeel,...
 
Red Blood Cells_anemia & polycythemia.pdf
Red Blood Cells_anemia & polycythemia.pdfRed Blood Cells_anemia & polycythemia.pdf
Red Blood Cells_anemia & polycythemia.pdf
 
Role of Soap based and synthetic or syndets bar
Role of  Soap based and synthetic or syndets barRole of  Soap based and synthetic or syndets bar
Role of Soap based and synthetic or syndets bar
 
American College of physicians ACP high value care recommendations in rheumat...
American College of physicians ACP high value care recommendations in rheumat...American College of physicians ACP high value care recommendations in rheumat...
American College of physicians ACP high value care recommendations in rheumat...
 
CPR.nursingoutlook.pdf , Bsc nursing student
CPR.nursingoutlook.pdf , Bsc nursing studentCPR.nursingoutlook.pdf , Bsc nursing student
CPR.nursingoutlook.pdf , Bsc nursing student
 
Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024
 
ayurvedic formulations herbal drug technologyppt
ayurvedic formulations herbal drug technologypptayurvedic formulations herbal drug technologyppt
ayurvedic formulations herbal drug technologyppt
 
AUTONOMIC NERVOUS SYSTEM organization and functions
AUTONOMIC NERVOUS SYSTEM organization and functionsAUTONOMIC NERVOUS SYSTEM organization and functions
AUTONOMIC NERVOUS SYSTEM organization and functions
 
Male Infertility Panel Discussion by Dr Sujoy Dasgupta
Male Infertility Panel Discussion by Dr Sujoy DasguptaMale Infertility Panel Discussion by Dr Sujoy Dasgupta
Male Infertility Panel Discussion by Dr Sujoy Dasgupta
 
SGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdf
SGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdfSGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdf
SGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdf
 
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdfPAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
 
Rheumatoid arthritis Part 1, case based approach with application of the late...
Rheumatoid arthritis Part 1, case based approach with application of the late...Rheumatoid arthritis Part 1, case based approach with application of the late...
Rheumatoid arthritis Part 1, case based approach with application of the late...
 
Basic structure of hair and hair growth cycle.pptx
Basic structure of hair and hair growth cycle.pptxBasic structure of hair and hair growth cycle.pptx
Basic structure of hair and hair growth cycle.pptx
 
How to master Steroid (glucocorticoids) prescription, different scenarios, ca...
How to master Steroid (glucocorticoids) prescription, different scenarios, ca...How to master Steroid (glucocorticoids) prescription, different scenarios, ca...
How to master Steroid (glucocorticoids) prescription, different scenarios, ca...
 
Biologic therapy ice breaking in rheumatology, Case based approach with appli...
Biologic therapy ice breaking in rheumatology, Case based approach with appli...Biologic therapy ice breaking in rheumatology, Case based approach with appli...
Biologic therapy ice breaking in rheumatology, Case based approach with appli...
 
historyofpsychiatryinindia. Senthil Thirusangu
historyofpsychiatryinindia. Senthil Thirusanguhistoryofpsychiatryinindia. Senthil Thirusangu
historyofpsychiatryinindia. Senthil Thirusangu
 
Mental health Team. Dr Senthil Thirusangu
Mental health Team. Dr Senthil ThirusanguMental health Team. Dr Senthil Thirusangu
Mental health Team. Dr Senthil Thirusangu
 
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptxDNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
 
EXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionEXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung function
 
power point presentation of Clinical evaluation of strabismus
power point presentation of Clinical evaluation  of strabismuspower point presentation of Clinical evaluation  of strabismus
power point presentation of Clinical evaluation of strabismus
 

Cellular carriers ppt

  • 1. PRESENTED BY: STUTI TRIPATHI M.PHARM 2nd SEM.
  • 3. Resealed erythrocytes are prepared by collecting blood samples from the organism of interest and separating erythrocytes from plasma. Resealed erythrocytes are biocompatible, biodegradable, having long circulation half-life and can be loaded with variety of active substances.
  • 4. By using various methods (discuss later),cells are broken. Drug is entrapped into erythrocytes, and then they are resealed. Resultant carriers are termed as “Resealed erythrocytes”.
  • 5. PROPERTIES OF RESEALED ERYTHROCYTES: The drug should be released at target site in a controlled manner. It should be appropriate size, shape and should permit the passage through capillaries and minimum leakage of drug should take place. It should be biocompatible and have minimum toxic effect. The carrier system should have an appreciable stability during storage. It should have the ability to carry a broad spectrum of drug. The degradation product of the carrier system, after release of the drug at selected site should be biocomaptible.
  • 6. HYPO-OSMOTIC LYSIS METHOD: Hypotonic lysis of cells in a solution containing the drug to be entrapped followed by restoration of tonicity and reseal them. The ghost population so obtained are heterogeneous. Three types of ghosts can be distinguished : a. Type 1:ghosts which reseal immediately after haemolysis, b. Type 2:ghosts which reseal after reversal of haemolysis by addition of alkali ions, and c. Type 3:ghosts which remain leaky under different experimental conditions.
  • 7. Sr. No. Species Washing buffer Centrifugal force(g) 1. Rabbit 10mmol Phosphate buffer 500-1000 2. Dog 15mmol Phosphate buffer 500-1000 3. Human 154mmol NaCl <500 4. Cow 10-15mmol Phosphate buffer 1000 5. Mouse 10mmol Phosphate buffer 100-500 6. Horse 2mmol Magnesium chloride,10mmol glucose 1000 7. Sheep 10mmol Phosphate buffer 500-1000 8. Pig 10mmol Phosphate buffer 500-1000
  • 8. CHEMICAL PERTURBATION OF THE MEMBRANE: This method is based on the increase in membrane permeability of erythrocytes when the cells are exposed to certain chemicals. This method was used successfully by Kitao and Hattori to entrap the antineoplastic drug “daunomycin” in human and mouse erythrocytes.
  • 9. ELECTRO-INSERTION OR ELECTROCAPSULATION: This method is also known as electroporation, based on the observation that electrical shock brings about irreversible changes in an erythrocyte membrane. The erythrocyte membrane is opened by a dielectric breakdown. Subsequently, the pores can be resealed by incubation at 37⁰c in an isotonic medium.
  • 10. CHARACTERIZATION OF RESEALED ERYTHROCYTES: DRUG-CONTENT DETERMINATION: Packed loaded cells are deprotenized with acetonitrile after centrifugation at 300 rpm for a fixed time interval. The clear supernatant liquid is assayed for drug content. IN-VITRO DRUG RELEASE AND Hb CONTENT: The cell suspensions are stored at 4⁰c in ambered colored glass container. Periodically clear supernatant are drawn using a hypodermic syringe, filter and deproteined with methanol and then estimated for drug content. %Hb release= A540 of sample – A540 of background
  • 11. % CELL RECOVERY: May be determined by counting the no. of intact cells per cubic mm of packed erythrocytes before and after loading the drug. ERYTHROCYTE SEDIMENTATION RATE: It is an estimate of the suspension stability of RBC in plasma and is related to the no. and size of the red cells and to relative concentration of plasma protein, especially fibrinogen and alpha and beta globulins. This test is performed by determining the rate of sedimentation of blood cells in a standard tube. NORMAL BLOOD ESR=0-15 mm/hr.
  • 12. APPLICATIONS: Targeting of bioactive agents to RE system Targeting to sites other than RES-rich organs Erythrocytes as circulating bioreactors Erythrocytes as carriers for enzymes
  • 13. APPLICATION DRUG/ENZYME/MACROMOLECULE Enzyme deficiency replacement therapy ẞ- galactosidase , ẞ- fructofuronodase,urease,glucose-6- phosphatedehydrogenase Thrombolytic activity Brinase , aspirin , heparin Iron overload Desferroxamine Immunotherapy Human recombinant interlukin-2
  • 14. REFERNCES Jain S.K. and Vyas S.P., “Magnetically Responsive Diclofenac Sodium- Loaded Erythrocytes: Preparation and In-Vitro Characterization, CBS Publishers and Distributors, pg. no.-141-51. Vyas S.P. and Dixit V.K., Pharmaceutical Biotechnology , CBS Publishers & Distributors, New Delhi, pg. no. 655