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Poster presentations Unni Sep292011


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Poster presentations Unni Sep292011

  1. 1. Elizabeth Unni Sep 29, 2011
  2. 2. Why?• Opportunity to exchange ideas Clinical problems Research outcomes Research ideas (Work in progress)Where?• Conferences (Eg: Diabetes Summit)• Professional Association Annual Meetings National – APhA Local – UPhA• Capitol Hill Policy making E. Unni 9/29/2011 2
  3. 3. TitleAbstract (Optional) It is not necessary toIntroduction/Background have all theseGoals/Objectives components in a posterMethodsResultsDiscussion & LimitationsClinical ImplicationsConclusionAcknowledgementsReferences E. Unni 9/29/2011 3
  4. 4. Use the 10–10 rule• Attendees spend only 10 seconds scanning posters as they stroll by from a distance of 10 feet• Essential that the poster capture their attentionBlend between manuscript and oralpresentation• Use content judiciously E. Unni 9/29/2011 4
  5. 5. Clarity & Simplicity• Concise and neat layoutKeep the words to a minimum• Busy attendees do not have time to read a lotUse pictures, graphs, and diagrams• But not in a distractive wayMain message should be clear and visible• Resist the urge to fill voids with clutter that might discourage attendees from approaching your poster E. Unni 9/29/2011 5
  6. 6. Use phrases, not sentencesUse bullet points, not paragraphsAvoid jargon and acronymsUse consistent wording, especiallybetween text and visualsEnsure that the content is self-explanatory E. Unni 9/29/2011 6
  7. 7. Ensure that the text is legible andappealing• Use the same type-font throughout the poster Times New Roman or Arial is usually used• Distinguish between headers and text Size of font Headers can be read from 5m and text can be read from 2m. Type of font Universal for headers• Top to bottom & left to right reading Logical sequence of presentation• Softer colors (pastel or gray) as background E. Unni 9/29/2011 7
  8. 8. Suggested font sizes:• Title: 96 pt• Authors: 72 pt• Affiliations: 36-48 pt• Section headings: 36 pt• Text: 24 pt• Acknowledgements: 18 pt E. Unni 9/29/2011 8
  9. 9. Editing• Proofread Colleagues People familiar with your topic People not so familiar with your topic• Spell check Very very.. important• Get feedback E. Unni 9/29/2011 9
  10. 10. Components• Title Interesting – to capture attention of the audience If too long, shorten it; do not reduce font size• Authors If too many authors, use last and first name Omit middle initials & titles Include academic affiliation Omit city and state if there are too many authors & gives a crowded look E. Unni 9/29/2011 10
  11. 11. Components• Introduction/background Research gap What is the research question? Why is this research question important? Should lead to the goals/objectives• Methods What was the strategy used? Why was this strategy used? E. Unni 9/29/2011 11
  12. 12. Components• Results The most important part of a poster Do not assume everyone is an expert in your field What are the results? Use graphs, tables, and diagrams Include only the most important & unique results• Discussion/conclusion Summary of findings in a sentence or two How does your results compare with existing research? What is the next step of the study? E. Unni 9/29/2011 12
  13. 13. Components• References Omit if possible Include it in the handout• Acknowledgements Research partners, funding source If space allows, provide your email ID or QRC code, or a website where they can access the poster E. Unni 9/29/2011 13
  14. 14. Usually 3 to 4 columnsEither• Equal distribution of columns• Middle column wider than the other two columns Depending on content• Equal spacing between columns• All the top headers on the same level E. Unni 9/29/2011 14
  15. 15. Know your poster and contents well• Should be able to explain the complete poster in 3 to 5 minutes Practice it and time it!• Have a good opening sentence Explains why your poster is important Focus on the major question you are answering with the study• Explain your results & conclusions well and its importance What is the implication of your results? In clinical application In scientific world E. Unni 9/29/2011 15
  16. 16. Presentation style• Dress business casual/formals• Eye contact• Posture• Speak slowly and clearly Avoid jargons and acronyms Remember, not everyone is an expert in your field E. Unni 9/29/2011 16
  17. 17. Frame possible questions• prepare answersListen to the question• Wait until they finish the questionAnswer the question• Ask whether you answered their questionHave handouts with contact information E. Unni 9/29/2011 17
  18. 18. Budget it! Posters are expensive! E. Unni 9/29/2011 18
  19. 19. E. Unni 9/29/2011 19
  20. 20. Thermal and Mechanical Responses in TRPV1-/- Mice with Site Specific Expression of TRPV1 Graduate Program in Physical Therapy & Rehabilitation Science Introduction Methods Results NPG (Control) NPGTRPV1 Re-expression of TRPV1 in the skin of TRPV1-/- mice restoresTRPV1+/+-like Transient receptor potential vanilloid 1 (TRPV1) is a mechanical sensitivity TK (UL23) hCMV EGFP PATK (UL23) Ca2+ permeable nonselective cation channel that is TK (UL23) hCMV EGFP PATK (UL23) 8 Mechanical Response 8 Re-expression TRPV1 8 Re-expression TRPV1 8 Re-expression TRPV1 into into Skin into Muscle Muscle and Skin activated by both exogenous and endogenous TRPV1+/+ TRPV1-/-+TRPV1* TRPV1-/-+TRPV1 TRPV1-/-+TRPV1 TRPV1-/- stimuli, including heat, low pH, anandamide, and ΔγL3 ΔγL3 6 6 TRPV1-/-+Control 6 TRPV1-/-+Control 6 TRPV1-/-+Control capsaicin. TRPV1 channels are found in the central 4.5 4.5 Responses (0-10) Responses (0-10) Response (1-10) Response (0-10) ΔγL3 ΔγL3 4.5 4.5 IR-L IR-L IR-S IR-S and the peripheral nervous system and are involved IR-L IR-L IR-S IR-S 4 4 4 4 in the transmission and modulation of pain, as well 10 10 10 as the integration of diverse painful stimuli. TRPV1 10 2 2 2 2 Force (mN) Force (mN) antagonists have shown efficacy in reducing Force (mN) Force (mN) 1 1 UL36 hCMV TRPV1 WPRE UL37 1 1 nociception from inflammatory and neuropathic Viral Constructs: NPG (Control) and NPGTRPV1 (TRPV1 re-expressing virus). These vectors do not express 0 0.1 0 0.1 0 0 0.1 animal models of pain. 0.1TRPV1 consists of 6 transmembrane segments ICP34.5 (deletion) or thymidine kinase (TK, UL23, insertional inactivation). Expression of enhanced green fluorescent 0.4 0.7 1.6 0.4 0.7 1.6 0.4 0.7 1.6 0.4 0.7 1.6and intracellular NH2- and COOH- termini. The protein (EGFP) is driven by the hCMV immediate-early enhancer-promoter. PA, polyadenylation signal; IR, internal log Force (mN) log Force (mN) log Force (mN) log Force (mN)channel is organized as a tetramer of homo or repeat; L, long; S, short. NPGTRPV1 is similar to NPG and a cassette for expression of rat TRPV1 is inserted betweenheteromeric subunits. Re-expression of TRPV1 in the skin of TRPV1-/- mice results in a significant decrease in the number of withdrawals the UL36 and UL37 genes. This transcription cassette contains a woodchuck hepatitis virus element (WPRE) to enhance similar to that observed in naïve TRPV1+/+ mice, *, p<0.05, repeated ANOVA. Re-expression in the muscle or both RNA stability and the hCMV enhancer-promoter. the skin and muscle has no effect on the number of withdrawals. Re-expression of TRPV1: Re-expression of TRPV1 in muscle and skin in TRPV1-/- mice restores heat sensitivity Purpose and Experimental Plan HSV viruses (NPG or NPGTRPV1) were injected in either A) Skin, B) Muscle, or C) Skin and Re-expression of TRPV1 Re-expression of TRPV1 Re-expression of TRPV1 Muscle. In sets A and C, each mouse was injected intradermally into the left paw with 20µl of 25 Heat Stimulus TRPV1+/+ (n=6) 25 into Skin 25 into Muscle 25 Into Muscle and Skin virus (107PFU/µl). In sets B and C, each mouse was injected with 20µl of virus (107PFU/µl) in TRPV1-/- (n=6) TRPV1-/-+TRPV1 (n=5) TRPV1-/-+Control (n=7) TRPV1-/-+TRPV1 (n=5) TRPV1-/-+TRPV1 (n=5)In the present study, we examined if TRPV1 was a peripheral initiator of muscle inflammatory TRPV1-/-+Control (n=7) TRPV1-/-+Control (n=7) 20 20 20 20 the left gastrocnemius muscle. Animals were allowed to recover for 3-4 weeks before behaviorhyperalgesia. We hypothesized that removal of TRPV1 would have an effect on thermal but not experiments were performed. 15 * 15 15 15 PWL (s) PWL (s) PWL (s) PWL (s)mechanical hyperalgesia after the development of muscle inflammation. To test if TRPV1 in skin * *or muscle was necessary for the development of thermal hyperalgesia, we re-introduced the Results 10 10 10 10expression of TRPV1 by injecting recombinant HSV expressing TRPV1 into the skin, muscle, 5 5 5 5and both skin and muscle of TRPV1-/- mice. Increased mechanical sensitivity and decreased heat sensitivity in TRPV1-/- Mice 8 0 0 0 0 Mechanical Response 115V 125V 135V 115V 125V 135V 115V 125V 135V 115V 125V 135V TRPV1+/+ (n=15) 25 Heat Stimulus Methods 6 TRPV1-/- (n=15) TRPV1+/+ (n=6) TRPV1-/- (n=6) The number of withdrawals to repeated mechanical Re-expression of TRPV1 in both the muscle and skin in TRPV1-/- mice results in a significant decrease in the withdrawal latency to high intensity heat stimuli *, p=0.04, one-way ANOVA. Re-expression singly in either the skin or 20 stimulation was significantly increased in TRPV1-/- mice the muscle has no effect on the withdrawal latency. Response (1-10) compared to TRPV1+/+ mice. However, mechanicalAnimals: thresholds were similar between groups (inset). The latency Re-expression of TRPV1 in muscle and skin in TRPVI-/- mice restores 15 * PWL (s) 4 to high intensity thermal stimuli was increased in TRPV1-/-All animal experiments were approved by the University of Iowa Animal Care and Use * heat hyperalgesia after muscle inflammation 10 mice when compared to TRPV1+/+ mice, *, p<0.05. 10Committee and were conducted in accordance with National Institutes of Health guidelines. 120 TRPV1-/-+TRPV1 2 TRPV1-/-+Control Force (mN)Congenic TRPV1-/- on a C57Bl/6 background and C57Bl/6 (WT) mice were bred at the 1 5 110University of Iowa Animal Care Facility. Male mice, 6-10 weeks of age, were used in these Re-expression of TRPV1 in both the muscle and skin in 100 Percent Baseline TRPV1-/- mice restores the heat hyperalgesia (125V)studies. 0 0.1 0 90 induced by carrageenan-induced muscle inflammation. Re- 0.4 0.7 1.6 115V 125V 135V expression singly in either the skin or the muscle has noInduction of Inflammation: log Force (mN) 80 * effect on restoring the decreased withdrawal latency Loss of heat, but not mechanical hyperalgesia in TRPV1-/- mice with muscle inflammation 70 produced by carrageenan-induced muscle inflammation. *,Mice were briefly anesthetized with 4% isofluorane and the left gastrocnemius muscle was 60 p=0.01, one-way ANOVA.injected with 20 μl of 3% carrageenan. Behavior measurements were made before and 24, 48, TRPV1+/+, Ipsilateral (n=15) 18 TRPV1+/+,Ipsilateral (n=15) TRPV1+/+,Contralateral (n=15) 5072 hours, 1 week and 2 weeks after carrageenan injection. In the re-expresssion experiments, 5 TRPV1+/+, Contralateral (n=15) TRPV1-/-, Ipsilateral (n=15) Skin Muscle Muscle TRPV1-/-, Ipsilateral (n=15) TRPV1-/-, Contralateral (n=15) andbehavior measurements were made before and 24 hours after carrageenan injection. TRPV1-/-, Contralateral (n=15) 16 Skin 4 *** * * 14 Summary and Conclusions Responses (0-5) PWL (s)Behavioral Testing: 3 * * * * 12Mechanical sensitivity was tested bilaterally by assessing the number of responses to 2 * ** * * * 10 • TRPV1-/- mice show a higher level of baseline mechanical sensitivity of the paw thanrepeated application of von Frey filaments (0.4mN, 0.7mN, and 1.6mN) to the plantar surface of ** * * WT mice; re-expression of TRPV1 in the skin restores normal mechanical sensitivity. 1the paw. The number of withdrawals out of 5 was assessed in 10 trials and an average of all 10 8 * * * • TRPV1-/- mice show a lower level of baseline heat sensitivity than WT mice; re-expressiontrials was determined for each time period. An increase in the number of responses was 0 6 *interpreted as increased mechanical sensitivity of the paw. Base 72h 1wk 2wk Base 72h 1wk 2wk of TRPV1 in the muscle and the skin restores normal heat sensitivity. The number of withdrawals to repeated mechanical stimulation (0.4 mN) was significantly increased after carrageenan-Thermal sensitivity was tested bilaterally by exposing the plantar surface of the paw to radiant induced muscle inflammation in both TRPV1-/- mice and TRPV1+/+ mice, *, p<0.05, repeated ANOVA. However, theheat with thermal intensities corresponding to 125, 135, and 145V and recording the time in sec latency to heat (125V) was unchanged after muscle inflammation in TRPV1-/- mice compared to decreases observed in • WT and TRPV1-/- mice develop a similar increase in mechanical sensitivity of the paw after TRPV1+/+ mice, *, p<0.01, paired t-test from baseline. muscle inflammation.until withdrawal. Withdrawal times are expressed as an average of 3 trials per paw. Increased expressions of TRPV1 mRNA in DRGs of TRPV1-/- mice injected withQuantitative RT-PCR: HSV-1 expressing TRPV1 in skin, muscle, or skin and muscle • TRPV1-/- mice do not develop heat hyperalgesia after muscle inflammation; re-expression of 500 TRPV1 in muscle and skin restores the heat hyperalgesia induced by muscle inflammation. TRPV1 expression (arbitrary units)RNA was purified from ipsilateral and contralateral L4, L5, and L6 DRGs using the Trizolreagent (Invitrogen, Carlsbad, CA). First strand cDNA was synthesized from 0.2-1µg of each 400 Skin Muscle Quantitative PCR shows that injection of HSV-1 expressing • TRPV1 expression in the skin and muscle play distinct roles in the development ofRNA sample using VILO reverse transcriptase (Invitrogen, Carlsbad, CA). Taqman PCR was 300 Skin + Muscle TRPV1 into TRPV1-/- mice results in expression of rat TRPV1 mechanical and heat sensitivity and response to inflammatory muscle hyperalgesia.carried out using an ABI prism 7900 sequence detector on cDNA samples (University of Iowa, mRNA in DRG 4 weeks after injection. The increases areDNA Facility, Iowa City, IA). Reactions were carried out for 40 cycles in triplicate. Rat TRPV1 200 observed only on the side of injection and not on the contralateral side, Indicating new expression of TRPV1 in • TRPV1 plays a role in the responsiveness of cutaneous nociceptors to mechanical(Rn01460299_m1), and the mouse control assay for glyceraldehyde-3-P-dehydrogenase TRPV1-/- mice. Data represent means ± SEM; n=5-7 for all stimulation as well as to high intensity heat stimuli. 100(GAPDH) were obtained from Applied Biosystems, Inc. (Foster City, CA). Quantitative RT-PCR were normalized with GAPDH mRNA levels. 0 • TRPV1 expression in the skin and muscle is necessary for the restoration of heat sensitivity in TRPV1-/- mice. This suggests that TRPV1 is both a pH sensor in the ra si a ra si si ntr ip ip ip nt nt co co co M M S muscle and a heat sensor in the skin. S+ M S M S+ Supported by National Institutes of Health AR053509 and AR053509-S1. Too much text and too many results! E. Unni 9/29/2011 20
  21. 21. Professional?Interest capturing – Yes!! Strike a balance! E. Unni 9/29/2011 21
  22. 22. A poster with inappropriate proportions, spacing, & colors E. Unni 9/29/2011 22
  23. 23. Background is distracting! E. Unni 9/29/2011 23
  24. 24. Look at the column breaks… E. Unni 9/29/2011 24
  25. 25. Different background, but still soft.. Logical flow of contentJust enough pictures to keep it attractive E. Unni 9/29/2011 25
  26. 26. Graph and table colors not matching with theremaining text (Copy pasted without editing?) Last minute work? E. Unni 9/29/2011 26
  27. 27. Too much text!Will you stand there and read this complete? E. Unni 9/29/2011 27
  28. 28. Good pictures..BTW, what is the message? E. Unni 9/29/2011 28
  29. 29. THE β4 NICOTINIC RECEPTOR SUBUNIT MODULATES THE ANTIDEPRESSANT ACTION OF BUPROPION 1Radhakrishnan, R., 2DeBiasi, M., and 3Arias, H.R 1College of Pharmacy, University of Southern Nevada, South Jordan, UT., 2Baylor College of Medicine, Houston, TX., 3College of Pharmacy, Midwestern University, Glendale, AZ. Background Results Results Bupropion (BP) is an antidepressant drug used also as a smoking * * cessation agent. * Bupropion * * * BP is a noncompetitive inhibitor of several nicotinic acetylcholine receptors (AChRs). * * AChR inhibition could be involved in the antidepressant and anti-nicotinic activity of BP. Aims To determine the acute and chronic antidepressant effects of BP, and the withdrawal effects after chronic administration in male and female wild-type (β4+/+) and knockout (β4-/-) mice using the forced swim test. * * Methods * * β4 subunit of AChR is absent in * * * (β4-/-) mice and hence the receptor is assumed to be * dysfunctional. Wild-type (β4+/+) and KO (β4-/-) mice were Bullet separated by sex, and then injected (i.p.) with saline (0.9%points in NaCl; control) or BP (40 mg/kg) i) Acute treatment increased the antidepressant effect of every day for two weeks. Tomethods determine the acute and chronic Ref: Cryan et al., Trends Pharmacol Sci.,.23 (5),2002. effects of BP, forced swimming BP in all mice types. However the kinetics in β4-/- mice was faster than in β4+/+ mice. section tests were performed on the 1st day at 0, 15, 30, 45, and 60 min after the ii) The antidepressant effect after chronic treatment was seen only in the male and female β4+/+ mice, not in β4-/- Conclusions mice . The β4 subunit plays a modulatory role in: injection, and after two weeks of treatment, • the acute antidepressant effect of BP respectively. To determine the withdrawal effect, iii) Only male β4+/+ mice showed significant antidepressant • the chronic antidepressant effect of BP • the residual effect after withdrawal of chronic BP forced swimming tests were performed on the 1st effect compared to control mice after 1 week withdrawal. and the 2nd weeks after chronic treatment. BP may affect males and females differently. A good example of a basic science research poster E. Unni 9/29/2011 29
  30. 30. A good example of a clinical poster E. Unni 9/29/2011 30
  31. 31. Not too fancy, but professional! E. Unni 9/29/2011 31
  32. 32. Poster presented by P3 students for the Capitol Hill Pharmacy Day presentation E. Unni 9/29/2011 32
  33. 33. If you are on a budget issue, this is an option.. E. Unni 9/29/2011 33
  34. 34. Questions?E. Unni 9/29/2011 34