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Bacillus anthracis

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Hari, Thoothukudi Govt. Medical College, Thoothukudi
Hari, Thoothukudi Govt. Medical College, Thoothukudi

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BACILLUS ANTHRACIS G.HARIPRASAD M.Sc.,(Med micro), M.phil., Lecturer in Microbiology Department of Microbiology Thoothukudi Govt. Medical College Thoothukudi
[object Object],[object Object],[object Object],[object Object],[object Object],Cause of anthrax BACILLUS ANTHRACIS (GRAM STAIN)
Resistance ,[object Object],[object Object],[object Object],[object Object],[object Object]
ANTIGENS ,[object Object],[object Object],Other antigens: Cell wall polysaccharide antigens Somatic protein antigens
1. Polypeptide capsule – antiphagocytic 2. Anthrax toxin Multicomponent anthrax toxin Factor I – Edema factor Factor II – Protective factor Factor III – Lethal factor Toxin binds with susceptible cells through factor II Factor I & III enters the cell Factor I – causes edema by rising cAMP levels. Factor III – Kills the cell finally.
[object Object],[object Object],[object Object],[object Object]
HUMAN ANTHRAX ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
CUTANEOUS ANTHRAX ,[object Object],[object Object],[object Object],[object Object]
 
PULMONARY ANTHRAX ,[object Object],[object Object]
INTESTINAL ANTHRAX ,[object Object],[object Object],All clinical conditions lead to septicaemic anthrax if not treated early – Highly fatal!!!
Laboratory diagnosis ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],Gram stain McFadyaen’s reaction Spore stain
SELECTIVE MEDIA – PLET MEDIUM –POLYMYXIN, LYSOZYME, EDTA & THALLOUS ACETATE Culture
Culture ,[object Object],[object Object],[object Object]
Gelatin liquefaction Maximum liquefaction on the surface than at the bottom INVERTED FIR TREE APPEARANCE
Nitrate reduction test Nitrate negative Nitrate positive
[object Object],[object Object],[object Object],[object Object]
TREATMENT ,[object Object],[object Object],[object Object],ANIMAL VACCINE – Sterne vaccine HUMAN VACCINE – Alum Precipitated Toxoid – 3 doses intramuscularly at intervals of 6 weeks and 6 months Booster dose after one year.

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Bacillus anthracis

  • 1. BACILLUS ANTHRACIS G.HARIPRASAD M.Sc.,(Med micro), M.phil., Lecturer in Microbiology Department of Microbiology Thoothukudi Govt. Medical College Thoothukudi
  • 2.
  • 3.
  • 4.
  • 5. 1. Polypeptide capsule – antiphagocytic 2. Anthrax toxin Multicomponent anthrax toxin Factor I – Edema factor Factor II – Protective factor Factor III – Lethal factor Toxin binds with susceptible cells through factor II Factor I & III enters the cell Factor I – causes edema by rising cAMP levels. Factor III – Kills the cell finally.
  • 6.
  • 7.
  • 8.
  • 9.  
  • 10.
  • 11.
  • 12.
  • 13.
  • 14. SELECTIVE MEDIA – PLET MEDIUM –POLYMYXIN, LYSOZYME, EDTA & THALLOUS ACETATE Culture
  • 15.
  • 16. Gelatin liquefaction Maximum liquefaction on the surface than at the bottom INVERTED FIR TREE APPEARANCE
  • 17. Nitrate reduction test Nitrate negative Nitrate positive
  • 18.
  • 19.