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BACTERIAL STAINING G.HARIPRASAD M.Sc.,(Med micro),M.phil., Lecturer in Microbiology  Department of Microbiology  Thoothukudi Govt. Medical College  Thoothukudi
[object Object],[object Object],[object Object],INTRODUCTION:
[object Object],[object Object],[object Object],[object Object],[object Object],INTRODUCTION - STAINS
[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Principle of staining:
[object Object],[object Object],[object Object],[object Object],[object Object],Basic requirements for staining:
[object Object],[object Object],Basic initial steps before staining:
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],SIMPLE STAINING:
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Procedure:
GRAM STAINING : ,[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],REQUIREMENTS – STAINING REAGENTS:
[object Object],[object Object],[object Object],[object Object],PRINCIPLE:
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],RESULT:
GRAM POSITIVE COCCI IN CLUSTERS
GRAM POSITIVE COCCI IN CHAINS
GRAM NEGATIVE BACILLI
GRAM POSITIVE BACILLI
GRAM NEGATIVE COCCI
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],PROCEDURE:
Gram stain (Mixed)
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],EXAMPLES:
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],ACID-FAST STAINING: (Ziehl-Neelsen stain)
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Staining reagents:
[object Object],[object Object],[object Object],[object Object],Procedure:
[object Object],[object Object],[object Object],[object Object],[object Object],Result:
Acid fast stain:
[object Object],SPECIAL STAIN:
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
CAPSULE STAIN BY NIGROSIN INK (BLACK)
CAPSULE STAIN- INDIAN INK(BLUE)
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Spore stain
[object Object],FLAGELLAR STAIN – SILVER STAIN:
[object Object],[object Object],[object Object],[object Object]

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Bacterial staining